BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021690 (673 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g19220.1 68414.m02392 transcriptional factor B3 family protei... 30 1.2 At4g09730.1 68417.m01598 DEAD/DEAH box helicase, putative RNA he... 29 2.8 At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-g... 28 4.9 At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)... 27 8.6 >At1g19220.1 68414.m02392 transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related contains Pfam profile: PF02309 AUX/IAA family Length = 1086 Score = 30.3 bits (65), Expect = 1.2 Identities = 19/75 (25%), Positives = 38/75 (50%) Frame = +3 Query: 105 SLVHQYLEKIDKSLAQTFMKKTKAKPRAKNQQTLLDIIAKFNQANKAKAEVPVTRAKKMP 284 S +H + + ++ Q M++ +KP + +QQ L ++ K Q + ++ PV+ + + Sbjct: 615 SSLHSLQQNLSQNPQQLQMQQQSSKP-SPSQQLQLQLLQKLQQQQQQQSIPPVSSSLQPQ 673 Query: 285 LRNQQFKQMGKFQQL 329 L Q Q + QQL Sbjct: 674 LSALQQTQSHQLQQL 688 >At4g09730.1 68417.m01598 DEAD/DEAH box helicase, putative RNA helicase -Mus musculus,PIR2:I84741 Length = 621 Score = 29.1 bits (62), Expect = 2.8 Identities = 12/41 (29%), Positives = 18/41 (43%) Frame = +2 Query: 293 PTIQTNGKVPATKKKAEXXXXXXXXXEPPKQASKPNQAVKK 415 P T GK P K + PP+++SKP + + K Sbjct: 543 PARSTGGKTPVRKSSSSSFSKPRKASSPPEKSSKPKRKILK 583 >At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-glucan glucanohydrolase, putative similar to SP|P17859 Alpha-amylase precursor (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase) {Vigna mungo}, alpha-amylase [Malus x domestica] GI:7532799; contains Pfam profile PF00128: Alpha amylase, catalytic domain Length = 887 Score = 28.3 bits (60), Expect = 4.9 Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 2/33 (6%) Frame = +2 Query: 527 YVHHKKK--RNHQMTIVVMMNLRKRFHKNQLLH 619 Y+ H K R ++ I + +NLR F N+LLH Sbjct: 14 YLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLH 46 >At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5) identical to heat shock protein 70 [Arabidopsis thaliana] GI:6746590 Length = 682 Score = 27.5 bits (58), Expect = 8.6 Identities = 22/79 (27%), Positives = 35/79 (44%), Gaps = 5/79 (6%) Frame = +3 Query: 36 EIH*ILDKNFKMNLTTEIQAD-----VNSLVHQYLEKIDKSLAQTFMKKTKAKPRAKNQQ 200 E+H DK K + T+ AD + + +Y EKI +A+ A + Sbjct: 569 ELHAQKDKERKELIDTKNTADTTIYSIEKSLGEYREKIPSEIAKEIEDAVADLRSASSGD 628 Query: 201 TLLDIIAKFNQANKAKAEV 257 L +I AK ANKA +++ Sbjct: 629 DLNEIKAKIEAANKAVSKI 647 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,791,443 Number of Sequences: 28952 Number of extensions: 170720 Number of successful extensions: 543 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 534 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 543 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1422784080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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