BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021688 (685 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces... 77 2e-15 SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 27 1.9 SPCC61.03 |||conserved protein|Schizosaccharomyces pombe|chr 3||... 25 7.7 >SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 796 Score = 77.0 bits (181), Expect = 2e-15 Identities = 31/88 (35%), Positives = 56/88 (63%) Frame = +3 Query: 243 VFGYDETYFILKYNAAVVTRARETNTDITEDGIEDAFEVVGEVNETVKTGIWVGDCFIYT 422 + D+ +++L +NA + A E+ T E+G+ D+FE + +V+E+V G WV + FIYT Sbjct: 478 ILACDDDFYLLGFNAEMFYSAVESGTADEEEGVADSFEALADVSESVVNGKWVAETFIYT 537 Query: 423 NTLNRINYYVGGEIVTVAHLDHTMYILG 506 T R+NY +G + +A+++ + Y+LG Sbjct: 538 TTAARLNYLIGDQTYKIANVESSFYLLG 565 Score = 71.3 bits (167), Expect = 1e-13 Identities = 32/84 (38%), Positives = 50/84 (59%) Frame = +1 Query: 1 DSSEYATLENSSSVKVFKNFKERKSFKPEYGAEGIFGGYMLGVKSISGVAFSFYDWEHLE 180 DS+ Y + + S+ + KNFKE Y + IFGG++LGV + FYDW+ Sbjct: 399 DSNVYGSRTSDRSIVIHKNFKESNRLDLSYSCDKIFGGFLLGV--VGSDFICFYDWDTGI 456 Query: 181 LVRRIEIQPRHVFWSESGNLVCLA 252 LVR+I+++P+ V+W++ G V LA Sbjct: 457 LVRKIDVKPKGVYWNDDGRFVILA 480 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/54 (31%), Positives = 30/54 (55%) Frame = +2 Query: 503 GIVAKENRLYLNDKELNIXXXXXXXXXXEYQTAVMRGDFETADRVLPTIPTEHR 664 G + +++R+YL D+++N+ EYQ+ V++GD E A +L I R Sbjct: 565 GYIPRDDRIYLTDRDMNVVSYSFNLAIIEYQSLVLKGDLEAAQGLLEQISETDR 618 >SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr 1|||Manual Length = 1854 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = -2 Query: 168 PIVERECYSADRLHAEHVASEYTFSTVLGLEA 73 PI ER+ D+ HA V +E F+T+ +EA Sbjct: 964 PIYERDHLLNDKQHAWFVWTEVAFATIFTIEA 995 >SPCC61.03 |||conserved protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 327 Score = 25.4 bits (53), Expect = 7.7 Identities = 20/74 (27%), Positives = 35/74 (47%), Gaps = 7/74 (9%) Frame = +3 Query: 162 RLGTFGTCQTY*D----SASSRVLVRKREPCVFGYDETYFILK-YNAAVVTR--ARETNT 320 R+G FG CQ Y S+ S +L + +F E ++K Y+ ++ RE + Sbjct: 33 RVGVFGGCQHYTGAPYYSSMSSMLFGSDQSHIFCEKEAANVIKSYSPDLIVHPFLREKDK 92 Query: 321 DITEDGIEDAFEVV 362 ED ++ FE++ Sbjct: 93 AGPEDSVDKCFELI 106 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,857,729 Number of Sequences: 5004 Number of extensions: 59265 Number of successful extensions: 143 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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