BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021677
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 103 3e-23
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 103 3e-23
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.5
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 4.3
SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 26 5.7
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 26 5.7
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 25 10.0
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 103 bits (246), Expect = 3e-23
Identities = 42/49 (85%), Positives = 45/49 (91%)
Frame = +2
Query: 254 SESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 400
+ESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH
Sbjct: 64 AESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWH 112
Score = 82.6 bits (195), Expect = 5e-17
Identities = 38/64 (59%), Positives = 48/64 (75%)
Frame = +3
Query: 63 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 242
++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AG
Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59
Query: 243 HQTS 254
HQTS
Sbjct: 60 HQTS 63
Score = 58.0 bits (134), Expect = 1e-09
Identities = 28/56 (50%), Positives = 40/56 (71%), Gaps = 1/56 (1%)
Frame = +3
Query: 510 LPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKVNAQ-RRRIQRK 674
+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGK + RR +QR+
Sbjct: 150 VPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRR 205
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/28 (57%), Positives = 22/28 (78%)
Frame = +1
Query: 433 LGGSVAATGVPALVQARGHIIEKIPGFP 516
+ +VAA+GVP+L+ ARGH IE+IP P
Sbjct: 124 IASAVAASGVPSLLLARGHRIEEIPEVP 151
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 103 bits (246), Expect = 3e-23
Identities = 42/49 (85%), Positives = 45/49 (91%)
Frame = +2
Query: 254 SESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 400
+ESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH
Sbjct: 64 AESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWH 112
Score = 83.4 bits (197), Expect = 3e-17
Identities = 39/64 (60%), Positives = 48/64 (75%)
Frame = +3
Query: 63 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 242
++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AG
Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59
Query: 243 HQTS 254
HQTS
Sbjct: 60 HQTS 63
Score = 57.6 bits (133), Expect = 2e-09
Identities = 28/56 (50%), Positives = 40/56 (71%), Gaps = 1/56 (1%)
Frame = +3
Query: 510 LPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKVNAQ-RRRIQRK 674
+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGK + RR +QR+
Sbjct: 150 VPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRR 205
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/28 (57%), Positives = 22/28 (78%)
Frame = +1
Query: 433 LGGSVAATGVPALVQARGHIIEKIPGFP 516
+ +VAA+GVP+L+ ARGH IE+IP P
Sbjct: 124 ISSAVAASGVPSLLLARGHRIEEIPEVP 151
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.1 bits (57), Expect = 2.5
Identities = 12/42 (28%), Positives = 17/42 (40%)
Frame = +1
Query: 364 YVRPHEALAALAPSRQPPTAESGLGGSVAATGVPALVQARGH 489
+ PH L + AP QP E +V P+ +GH
Sbjct: 854 FYEPHSYLESPAPEPQPSYEEESFNATVIHAPTPSTATFQGH 895
>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 606
Score = 26.2 bits (55), Expect = 4.3
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = -1
Query: 124 APCTVSLFSEYTDTKGRATDRLISLL 47
A C V ++SEYT G ++ L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515
>SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1842
Score = 25.8 bits (54), Expect = 5.7
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = +3
Query: 156 PIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSLNHG 266
P + +L+ +V + + +P+ SKEA H+ L HG
Sbjct: 1073 PEKKELLQEVVID---HDLEPFEASKEAAHEFKLRHG 1106
>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
3-phosphatidyltransferase Pis1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 25.8 bits (54), Expect = 5.7
Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%)
Frame = -2
Query: 81 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 4
K+G LP+ SF Y + +PL Y TL+AV+
Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 25.0 bits (52), Expect = 10.0
Identities = 11/48 (22%), Positives = 21/48 (43%)
Frame = +3
Query: 114 VQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSL 257
+QG+ P F + P+ + H ++ PY K+ H+ S+
Sbjct: 709 LQGSDGPRKFTIEKAGEPNKLPKAHTCFNRLDLPPYTSKKDLDHKLSI 756
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,584,706
Number of Sequences: 5004
Number of extensions: 49717
Number of successful extensions: 153
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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