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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021676X
         (418 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_UPI00015B551C Cluster: PREDICTED: similar to factor 5; ...    39   0.036
UniRef50_UPI0000D57222 Cluster: PREDICTED: similar to Transcript...    38   0.083
UniRef50_Q05913 Cluster: Transcription initiation factor IIF sub...    35   0.78 
UniRef50_Q4S5H9 Cluster: Chromosome 3 SCAF14730, whole genome sh...    34   1.0  
UniRef50_P35269 Cluster: Transcription initiation factor IIF sub...    33   2.4  
UniRef50_UPI000069FAC5 Cluster: UPI000069FAC5 related cluster; n...    33   3.1  
UniRef50_Q7ZV95 Cluster: General transcription factor IIF, polyp...    32   5.5  
UniRef50_Q17558 Cluster: Putative uncharacterized protein; n=2; ...    31   9.6  

>UniRef50_UPI00015B551C Cluster: PREDICTED: similar to factor 5;
           n=1; Nasonia vitripennis|Rep: PREDICTED: similar to
           factor 5 - Nasonia vitripennis
          Length = 526

 Score = 39.1 bits (87), Expect = 0.036
 Identities = 17/25 (68%), Positives = 19/25 (76%)
 Frame = +3

Query: 342 ASYTECGVTEEXVRRYLARKPMTTT 416
           A   E G+TE+ VRRYL RKPMTTT
Sbjct: 454 AGANESGITEDAVRRYLMRKPMTTT 478


>UniRef50_UPI0000D57222 Cluster: PREDICTED: similar to Transcription
           initiation factor IIF alpha subunit (TFIIF-alpha)
           (Transcription factor 5 large chain) (TF5A); n=1;
           Tribolium castaneum|Rep: PREDICTED: similar to
           Transcription initiation factor IIF alpha subunit
           (TFIIF-alpha) (Transcription factor 5 large chain)
           (TF5A) - Tribolium castaneum
          Length = 513

 Score = 37.9 bits (84), Expect = 0.083
 Identities = 16/29 (55%), Positives = 20/29 (68%)
 Frame = +3

Query: 330 PKLDASYTECGVTEEXVRRYLARKPMTTT 416
           P      +E G+TE+ VR+YL RKPMTTT
Sbjct: 439 PNYIVGSSESGITEDAVRKYLMRKPMTTT 467


>UniRef50_Q05913 Cluster: Transcription initiation factor IIF
           subunit alpha; n=5; Endopterygota|Rep: Transcription
           initiation factor IIF subunit alpha - Drosophila
           melanogaster (Fruit fly)
          Length = 577

 Score = 34.7 bits (76), Expect = 0.78
 Identities = 14/19 (73%), Positives = 16/19 (84%)
 Frame = +3

Query: 360 GVTEEXVRRYLARKPMTTT 416
           G+TEE VRRYL RKP+T T
Sbjct: 514 GITEEAVRRYLKRKPLTAT 532


>UniRef50_Q4S5H9 Cluster: Chromosome 3 SCAF14730, whole genome
           shotgun sequence; n=2; Tetraodontidae|Rep: Chromosome 3
           SCAF14730, whole genome shotgun sequence - Tetraodon
           nigroviridis (Green puffer)
          Length = 547

 Score = 34.3 bits (75), Expect = 1.0
 Identities = 15/26 (57%), Positives = 19/26 (73%)
 Frame = +3

Query: 336 LDASYTECGVTEEXVRRYLARKPMTT 413
           L+ +  +  +TEE VRRYL RKPMTT
Sbjct: 475 LEPTVNDVQLTEEAVRRYLIRKPMTT 500


>UniRef50_P35269 Cluster: Transcription initiation factor IIF
           subunit alpha; n=29; Eumetazoa|Rep: Transcription
           initiation factor IIF subunit alpha - Homo sapiens
           (Human)
          Length = 517

 Score = 33.1 bits (72), Expect = 2.4
 Identities = 14/17 (82%), Positives = 15/17 (88%)
 Frame = +3

Query: 363 VTEEXVRRYLARKPMTT 413
           VTE+ VRRYL RKPMTT
Sbjct: 454 VTEDAVRRYLTRKPMTT 470


>UniRef50_UPI000069FAC5 Cluster: UPI000069FAC5 related cluster; n=1;
           Xenopus tropicalis|Rep: UPI000069FAC5 UniRef100 entry -
           Xenopus tropicalis
          Length = 425

 Score = 32.7 bits (71), Expect = 3.1
 Identities = 15/33 (45%), Positives = 20/33 (60%)
 Frame = +3

Query: 15  TRGRAHEGRRRESQQAHQEKEEN*RN*KRFEQR 113
           TR R HEGR RE++Q   E+EE     +R  +R
Sbjct: 233 TRQRQHEGRERETRQRQHEREERRETRQRQHER 265


>UniRef50_Q7ZV95 Cluster: General transcription factor IIF,
           polypeptide 1; n=3; Danio rerio|Rep: General
           transcription factor IIF, polypeptide 1 - Danio rerio
           (Zebrafish) (Brachydanio rerio)
          Length = 443

 Score = 31.9 bits (69), Expect = 5.5
 Identities = 15/27 (55%), Positives = 19/27 (70%)
 Frame = +3

Query: 333 KLDASYTECGVTEEXVRRYLARKPMTT 413
           K   S ++  +TE+ VRRYL RKPMTT
Sbjct: 370 KSTPSASDVLLTEDAVRRYLIRKPMTT 396


>UniRef50_Q17558 Cluster: Putative uncharacterized protein; n=2;
           Caenorhabditis|Rep: Putative uncharacterized protein -
           Caenorhabditis elegans
          Length = 481

 Score = 31.1 bits (67), Expect = 9.6
 Identities = 16/30 (53%), Positives = 19/30 (63%)
 Frame = +3

Query: 324 KRPKLDASYTECGVTEEXVRRYLARKPMTT 413
           K+ K    + E G+ EE VRRYL RKP TT
Sbjct: 399 KKAKPAVKFEE-GLNEETVRRYLRRKPHTT 427


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 178,565,004
Number of Sequences: 1657284
Number of extensions: 1540059
Number of successful extensions: 5890
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 5453
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5886
length of database: 575,637,011
effective HSP length: 92
effective length of database: 423,166,883
effective search space used: 19465676618
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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