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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021674
         (701 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,...    30   1.7  
At3g52160.1 68416.m05726 beta-ketoacyl-CoA synthase family prote...    29   3.9  
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si...    29   3.9  
At3g62370.1 68416.m07006 expressed protein                             28   6.9  
At4g29310.1 68417.m04190 expressed protein                             27   9.1  
At3g47910.1 68416.m05224 expressed protein low similarity to non...    27   9.1  
At3g06510.1 68416.m00755 glycosyl hydrolase family 1 protein sim...    27   9.1  

>At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,
            putative similar to SP|Q03460 Glutamate synthase [NADH],
            chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT)
            {Medicago sativa}
          Length = 2208

 Score = 29.9 bits (64), Expect = 1.7
 Identities = 22/64 (34%), Positives = 27/64 (42%), Gaps = 1/64 (1%)
 Frame = +1

Query: 160  PRD-PVRGHCQAGSLTGAVHLSKNNAGVLRPAHEDRNLAWSKRAKAGLIQMFSTHRDCES 336
            PRD PV G   +G       L  N   +L   HED N   +K  K  +I    T  DC  
Sbjct: 1946 PRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKGKKVVVIGGGDTGTDCIG 2005

Query: 337  TAYR 348
            T+ R
Sbjct: 2006 TSIR 2009


>At3g52160.1 68416.m05726 beta-ketoacyl-CoA synthase family protein
           beta-ketoacyl-CoA synthase - Simmondsia
           chinensis,PID:g1045614
          Length = 451

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 11/27 (40%), Positives = 18/27 (66%)
 Frame = +1

Query: 283 RAKAGLIQMFSTHRDCESTAYRSFSIK 363
           RAK  L+Q+  TH+  E T+Y+S  ++
Sbjct: 291 RAKYQLMQLVRTHKGMEDTSYKSIELR 317


>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
           similarity to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501
          Length = 545

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
 Frame = -2

Query: 214 VPPQSNSPPGSVLE-PDHAGVLNGDE-RFRHVTTLHAWNET 98
           +P  SNS P +VL   + A  + GD     H TT+H W+ET
Sbjct: 5   LPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSET 45


>At3g62370.1 68416.m07006 expressed protein
          Length = 361

 Score = 27.9 bits (59), Expect = 6.9
 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
 Frame = -2

Query: 199 NSPPGSVL--EPDHAGVLNGDERFRHVTTLHAWN 104
           N+ PG +    P   G  NG +RF H+  ++AWN
Sbjct: 160 NAIPGRLYGGNPIDNGEGNGGDRFGHLVDIYAWN 193


>At4g29310.1 68417.m04190 expressed protein
          Length = 424

 Score = 27.5 bits (58), Expect = 9.1
 Identities = 15/54 (27%), Positives = 27/54 (50%)
 Frame = -1

Query: 458 IEGSKSNVAMNAWLPQASYPCGNFSGTSC*KLFILKDR*AVLSQSLCVLNIWIK 297
           I G K ++ ++ +  +  + CG  SG    K+ +  D  A LS+++   N W K
Sbjct: 85  ISGKKISLRVSVYAGRTGHTCGVASGKLLGKVEVAVDLAAALSRTVAFHNGWKK 138


>At3g47910.1 68416.m05224 expressed protein low similarity to
            nonmuscle myosin heavy chain (NMHC) [Homo sapiens]
            GI:189036; contains Pfam profiles PF04780: Protein of
            unknown function (DUF629), PF04781: Protein of unknown
            function (DUF627)
          Length = 1290

 Score = 27.5 bits (58), Expect = 9.1
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = -3

Query: 114  TLGTKHRAPADIIDRAPLPPNRVSNETMKVVV 19
            T+G+ HR+ AD ++ +PLP   V + +   +V
Sbjct: 972  TIGSDHRSNADSVEHSPLPVAPVGDHSEADIV 1003


>At3g06510.1 68416.m00755 glycosyl hydrolase family 1 protein
           similar to Beta-galactosidase (SP:P22498) [Sulfolobus
           solfataricus}; almost identical to beta-glucosidase
           GB:AAF23823 GI:6685165 from [Arabidopsis thaliana]
          Length = 622

 Score = 27.5 bits (58), Expect = 9.1
 Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
 Frame = +1

Query: 175 RGHCQAGSLTGAVHLSKNNAGVLRPAHEDRNLAWS---KRAKAGLIQMFSTHRDCESTAY 345
           R H  A +L  + HL        +   +DR+LAW+   K AKAG ++ F    D  +  Y
Sbjct: 490 RSHDLARTLRQSYHLFSKIVKSGKVTRKDRSLAWNELQKAAKAGKLRPFYRGVDNHNLMY 549


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,995,839
Number of Sequences: 28952
Number of extensions: 345791
Number of successful extensions: 870
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 846
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 870
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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