BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021673 (654 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g19150.1 68415.m02235 pectinesterase family protein contains ... 31 0.88 At3g56510.1 68416.m06284 TBP-binding protein, putative similar t... 29 2.0 At5g42370.1 68418.m05159 expressed protein 29 3.6 At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identic... 29 3.6 At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 4.7 At4g39050.1 68417.m05531 kinesin-related protein (MKRP2) kinesin... 28 6.2 At1g48960.1 68414.m05487 universal stress protein (USP) family p... 28 6.2 At5g22390.1 68418.m02612 expressed protein 27 8.2 At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 27 8.2 >At2g19150.1 68415.m02235 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 339 Score = 30.7 bits (66), Expect = 0.88 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +2 Query: 497 RRAPICGGVRILSADLENSGEGCTWRCRAGSYPYPQQV 610 +R I GG+ + ++ EGCT + R G YP P +V Sbjct: 181 KRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYP-PNEV 217 >At3g56510.1 68416.m06284 TBP-binding protein, putative similar to TBP-binding protein ABT1 GI:6518527 from [Mus musculus] Length = 257 Score = 29.5 bits (63), Expect = 2.0 Identities = 19/79 (24%), Positives = 32/79 (40%) Frame = -2 Query: 539 PQRVSGHRRKSVLFAFRTISPFYRIPWNSNAQAEKKTLPGPLGGVFRPLWVTPSNTRF*R 360 P + R + +L + + Y P +S AQ +K G G F WV + + Sbjct: 58 PPHMDHVRLRHILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAK 117 Query: 359 RGNDY*NGSAAGFRNRNRI 303 R D NG G + ++ + Sbjct: 118 RVADMLNGEQIGGKKKSSV 136 >At5g42370.1 68418.m05159 expressed protein Length = 447 Score = 28.7 bits (61), Expect = 3.6 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -2 Query: 479 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSNTR 369 P Y + + Q+ +K +P PL + R L W TPS R Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTMR 322 >At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identical to low affinity calcium antiporter CAX2 (GI:1488267) [Arabidopsis thaliana]; Ca2+:Cation Antiporter (CaCA) Family member PMID:11500563 Length = 441 Score = 28.7 bits (61), Expect = 3.6 Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%) Frame = +3 Query: 342 SIIVPSSLKTSVRRGNPKWPEDAAERSGKSFLFC--LSVRVPWNPI 473 S++ SL TS + PK P+++ S K +FC L++ +P+ P+ Sbjct: 41 SLMEQGSLSTSFPQHTPKAPKNSVLNSIKIVIFCNKLNLLLPFGPL 86 >At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein similar to SP|Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 676 Score = 28.3 bits (60), Expect = 4.7 Identities = 12/35 (34%), Positives = 20/35 (57%) Frame = -3 Query: 535 REYPDTAANRCSSRSEPYLPSIGFHGTRTLRQKRK 431 R Y + + R+ PY+P + F G RTL+ +R+ Sbjct: 275 RSYDMVSKDIIQRRTFPYVPDMKFSGNRTLKLERQ 309 >At4g39050.1 68417.m05531 kinesin-related protein (MKRP2) kinesin motor protein - Ustilago maydis, PID:g2062750; identical to cDNA MKRP2 mRNA for kinesin-related protein GI:16902293, kinesin-related protein [Arabidopsis thaliana] GI:16902294 Length = 1055 Score = 27.9 bits (59), Expect = 6.2 Identities = 18/47 (38%), Positives = 23/47 (48%) Frame = -1 Query: 183 TRNPSPRQSSRASLEYLLLPPRSAPTEASKRLTPRPFCALRRARPTR 43 +R PSP S+ +S L PRS T AS +T A R P+R Sbjct: 11 SRPPSPASSTSSSHLSNRLIPRSNSTSASSLITSAAGIASRSMTPSR 57 >At1g48960.1 68414.m05487 universal stress protein (USP) family protein contains Pfam PF00582: universal stress protein family; similar to hypothetical protein GI:7770340 from [Arabidopsis thaliana] Length = 219 Score = 27.9 bits (59), Expect = 6.2 Identities = 14/38 (36%), Positives = 18/38 (47%) Frame = -1 Query: 156 SRASLEYLLLPPRSAPTEASKRLTPRPFCALRRARPTR 43 S+ + L +P PT+ RL P P L R RP R Sbjct: 172 SQIEISGLQVPEIPTPTKVPYRLCPSPHAILWRTRPRR 209 >At5g22390.1 68418.m02612 expressed protein Length = 202 Score = 27.5 bits (58), Expect = 8.2 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = +3 Query: 414 ERSGKSFLFCLSVRVPWNPIEG 479 + S KSFL LS PWNP +G Sbjct: 17 DNSPKSFLDTLSSSSPWNPSKG 38 >At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140 [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis thaliana] Length = 926 Score = 27.5 bits (58), Expect = 8.2 Identities = 27/84 (32%), Positives = 36/84 (42%), Gaps = 5/84 (5%) Frame = -3 Query: 523 DTAANRCSSRSEPYLPSIGFHGTRTLRQKRKLFPDLSAASSGHFGLPRRTL---VFKDEG 353 D + R ++PYLPS G RTLR+K +L G L R V+ D G Sbjct: 219 DHPSKRILFTNQPYLPSETPSGLRTLREKE--LENLRGNGKGERKLSDRIYDYDVYNDIG 276 Query: 352 T--IIETVPLPGSGIGTGFPFAQR 287 I + P G G FP+ +R Sbjct: 277 NPDISRELARPTLG-GREFPYPRR 299 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,753,180 Number of Sequences: 28952 Number of extensions: 333116 Number of successful extensions: 931 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 904 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 931 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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