Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021376
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03340.1 68418.m00286 cell division cycle protein 48, putativ... 119 2e-27
At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)... 119 2e-27
At3g53230.1 68416.m05865 cell division cycle protein 48, putativ... 113 8e-26
At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak... 31 0.93
At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containi... 30 1.2
At1g69710.1 68414.m08022 zinc finger protein, putative / regulat... 29 2.8
At3g10160.1 68416.m01218 dihydrofolate synthetase/folylpolygluta... 28 5.0
At4g38750.1 68417.m05488 expressed protein 28 6.6
At5g10520.1 68418.m01218 protein kinase family protein contains ... 27 8.7
At5g05980.1 68418.m00662 dihydrofolate synthetase/folylpolygluta... 27 8.7
At1g49400.1 68414.m05537 ribosomal protein S17 family protein si... 27 8.7
At1g10180.1 68414.m01148 expressed protein 27 8.7
>At5g03340.1 68418.m00286 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain; supporting cDNA
gi|26449351|dbj|AK117125.1|
Length = 810
Score = 119 bits (286), Expect = 2e-27
Identities = 51/99 (51%), Positives = 67/99 (67%)
Frame = +2
Query: 221 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 400
L +G+ + CI L+D+ C + KIRM DV+S+ CP VKYGKRVHIL
Sbjct: 62 LIKGKKRKDTVCIALADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHIL 121
Query: 401 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFMSAG 517
P+DD+VEG+TGNLF+ YLKPYF+EAYRP+ + D F+ G
Sbjct: 122 PVDDTVEGVTGNLFDAYLKPYFLEAYRPVRKGDLFLVRG 160
Score = 93.9 bits (223), Expect = 9e-20
Identities = 42/65 (64%), Positives = 55/65 (84%)
Frame = +3
Query: 60 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 239
+D+K+ D STAIL RK PNRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKTKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPTTMEKLQLFRGDTILIKGKK 67
Query: 240 RKETV 254
RK+TV
Sbjct: 68 RKDTV 72
Score = 92.7 bits (220), Expect = 2e-19
Identities = 39/55 (70%), Positives = 49/55 (89%)
Frame = +1
Query: 508 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGG 672
VRGGMR+VEFKV+ETDP+ +C+VAPDT I C+GEP+KRE+EE L+ VGYDD+GG
Sbjct: 158 VRGGMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVKREDEER-LDEVGYDDVGG 211
>At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)
(CDC48) identical to SP|P54609 Cell division cycle
protein 48 homolog {Arabidopsis thaliana}
Length = 809
Score = 119 bits (286), Expect = 2e-27
Identities = 51/99 (51%), Positives = 67/99 (67%)
Frame = +2
Query: 221 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 400
L +G+ + CI L+D+ C + KIRM DV+S+ CP VKYGKRVHIL
Sbjct: 62 LIKGKKRKDTVCIALADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHIL 121
Query: 401 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFMSAG 517
P+DD+VEG+TGNLF+ YLKPYF+EAYRP+ + D F+ G
Sbjct: 122 PVDDTVEGVTGNLFDAYLKPYFLEAYRPVRKGDLFLVRG 160
Score = 96.7 bits (230), Expect = 1e-20
Identities = 44/65 (67%), Positives = 56/65 (86%)
Frame = +3
Query: 60 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 239
+D+KS D STAIL RK PNRL+V+EA++DDNSVV+L A ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKSKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPATMEKLQLFRGDTILIKGKK 67
Query: 240 RKETV 254
RK+TV
Sbjct: 68 RKDTV 72
Score = 92.3 bits (219), Expect = 3e-19
Identities = 39/55 (70%), Positives = 49/55 (89%)
Frame = +1
Query: 508 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGG 672
VRGGMR+VEFKV+ETDP+ +C+VAPDT I C+GEP+KRE+EE L+ VGYDD+GG
Sbjct: 158 VRGGMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVKREDEER-LDDVGYDDVGG 211
>At3g53230.1 68416.m05865 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain
Length = 815
Score = 113 bits (273), Expect = 8e-26
Identities = 47/99 (47%), Positives = 66/99 (66%)
Frame = +2
Query: 221 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 400
L +G+ + CI L+D+ C + KIRM DV+S+ CP VKYG RVHIL
Sbjct: 63 LIKGKKRKDTVCIALADETCDEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGNRVHIL 122
Query: 401 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFMSAG 517
P+DD++EG++GN+F+ YLKPYF+EAYRP+ + D F+ G
Sbjct: 123 PLDDTIEGVSGNIFDAYLKPYFLEAYRPVRKGDLFLVRG 161
Score = 92.7 bits (220), Expect = 2e-19
Identities = 39/55 (70%), Positives = 49/55 (89%)
Frame = +1
Query: 508 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGG 672
VRGGMR++EFKV+ETDP+ +C+VAPDT I C+GEPIKRE+EE L+ VGYDD+GG
Sbjct: 159 VRGGMRSIEFKVIETDPAEYCVVAPDTEIFCEGEPIKREDEER-LDEVGYDDVGG 212
Score = 84.2 bits (199), Expect = 7e-17
Identities = 40/66 (60%), Positives = 54/66 (81%), Gaps = 1/66 (1%)
Frame = +3
Query: 60 ADNK-SPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGK 236
+D+K + D STAIL +K NRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK
Sbjct: 8 SDSKGTKKDFSTAILEKKKAANRLVVDEAINDDNSVVSLHPDTMEKLQLFRGDTILIKGK 67
Query: 237 RRKETV 254
+RK+TV
Sbjct: 68 KRKDTV 73
>At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak
similarity to Pfam domain PF01612: 3'-5' exonuclease
Length = 263
Score = 30.7 bits (66), Expect = 0.93
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -1
Query: 212 TTEELKLLHFGL*KCHD*VVIAD 144
TTEELK+ H+ L KC D +V+A+
Sbjct: 3 TTEELKISHYKLYKCFDFLVVAN 25
>At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 455
Score = 30.3 bits (65), Expect = 1.2
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -2
Query: 367 RTRSDGYHIRKTHTKVVSHNTVHPNFLIRAII 272
RT S+ +++ HTK++ HN + L+R +I
Sbjct: 28 RTCSNFSQLKQIHTKIIKHNLTNDQLLVRQLI 59
>At1g69710.1 68414.m08022 zinc finger protein, putative / regulator
of chromosome condensation (RCC1) family protein similar
to zinc finger protein [Arabidopsis thaliana]
gi|15811367|gb|AAL08940
Length = 1028
Score = 29.1 bits (62), Expect = 2.8
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +3
Query: 462 TSWRLTVRSIVTTPSCPRGHARR 530
+ W+ T++ +T+ CP HARR
Sbjct: 127 SKWKTTIKPEITSAECPTPHARR 149
>At3g10160.1 68416.m01218 dihydrofolate
synthetase/folylpolyglutamate synthetase (DHFS/FPGS3)
nearly identical to gi:17976757
Length = 464
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 540 FELDGAHAPADMKVSSRW 487
F LDGAH+P M+ RW
Sbjct: 250 FYLDGAHSPESMEACGRW 267
>At4g38750.1 68417.m05488 expressed protein
Length = 1073
Score = 27.9 bits (59), Expect = 6.6
Identities = 13/55 (23%), Positives = 30/55 (54%), Gaps = 2/55 (3%)
Frame = +3
Query: 357 LRVLQ*NMENESTYCQLMIQLRVLLAI--YSKYT*SRTSWRLTVRSIVTTPSCPR 515
+++++ +EN+ + ++ L+ +LA Y KY W++T++ I +C R
Sbjct: 1 MQLVEGGLENDVVFALVVFSLQYILASHEYWKYNHGNMRWKVTLKVIELMKTCLR 55
>At5g10520.1 68418.m01218 protein kinase family protein contains
protein kinase domain, INTERPRO:IPR000719
Length = 467
Score = 27.5 bits (58), Expect = 8.7
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +1
Query: 55 KWQIIKALMIYR-PRSSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNF 198
++ +I L Y R ++ R P L+ +SA T +++ +P W NF
Sbjct: 95 RFSVIPLLASYELTRKNLRRKQP--KLTPSESAFTCEAFFMAKPSWRNF 141
>At5g05980.1 68418.m00662 dihydrofolate
synthetase/folylpolyglutamate synthetase (DHFS/FPGS2)
nearly identical to gi:17976705; identical to cDNA
dihydrofolate synthetase/folylpolyglutamate synthetase
(dhfs/fpgs2 gene) GI:17976704
Length = 571
Score = 27.5 bits (58), Expect = 8.7
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -3
Query: 540 FELDGAHAPADMKVSSRW 487
F LDGAH+P M+ ++W
Sbjct: 382 FYLDGAHSPESMEACAKW 399
>At1g49400.1 68414.m05537 ribosomal protein S17 family protein
similar to 40S ribosomal protein S17 GI:1620985 from
[Nicotiana plumbaginifolia]
Length = 116
Score = 27.5 bits (58), Expect = 8.7
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = +1
Query: 34 SNNKF*IKWQIIKALMIYRPRSSVARTDPTVSLSKKQSAMTTQS 165
S NK I +IIK IY P+++ A + S S SA TT S
Sbjct: 64 SKNKHWIVAEIIKKARIYSPKAAAAAVSASASAS---SASTTDS 104
>At1g10180.1 68414.m01148 expressed protein
Length = 769
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/33 (36%), Positives = 22/33 (66%)
Frame = +3
Query: 180 AKMEQLQLFRGDTVLLKGKRRKETVASCSQTII 278
+K++QL + GD +L K K +K +A ++T+I
Sbjct: 576 SKLQQLAIIAGDVLLGKEKLQKILLARLTETVI 608
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,693,584
Number of Sequences: 28952
Number of extensions: 307989
Number of successful extensions: 858
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 834
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 855
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -