Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021353
         (698 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC132.02 |hst2||Sir2 family histone deacetylase Hst2|Schizosac...   111   9e-26
SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo...    70   3e-13
SPAC1783.04c |hst4||Sir2 family histone deacetylase Hst4|Schizos...    43   5e-05
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy...    29   0.48 
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc...    29   0.48 
SPCC962.05 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||...    29   0.85 
SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase Rqh1|...    28   1.5  
SPBC3B9.14c |mrpl3||mitochondrial ribosomal protein subunit L3|S...    27   2.6  
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p...    27   2.6  
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi...    26   6.0  
SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces p...    26   6.0  
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p...    25   7.9  

>SPCC132.02 |hst2||Sir2 family histone deacetylase
           Hst2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 332

 Score =  111 bits (267), Expect = 9e-26
 Identities = 45/79 (56%), Positives = 64/79 (81%)
 Frame = +2

Query: 272 IKSDRCKKIITLSGAGISTSAGIPDFRSPETGLYHNLQKYELPQPQAIFEINFFRQNPKP 451
           IK  + KKI  + GAGIST+AGIPDFRSPETG+Y+NLQ++ LP  +A+F++++FR+NP+P
Sbjct: 22  IKEGKVKKICVMVGAGISTAAGIPDFRSPETGIYNNLQRFNLPYAEAVFDLSYFRKNPRP 81

Query: 452 FFTLAKELFPGSFKPTISH 508
           F+ LA EL P  ++PT +H
Sbjct: 82  FYELAHELMPEKYRPTYTH 100



 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 28/39 (71%), Positives = 34/39 (87%)
 Frame = +1

Query: 508 YFIRLLHEKGLLLRHYTQNIDTLERGAGIPEEKLVEAHG 624
           YFIRLLH+K LL + YTQNIDTLER AG+P++ L+EAHG
Sbjct: 101 YFIRLLHDKRLLQKCYTQNIDTLERLAGVPDKALIEAHG 139


>SPBC16D10.07c |sir2||Sir2 family histone deacetylase
           Sir2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 70.1 bits (164), Expect = 3e-13
 Identities = 33/75 (44%), Positives = 50/75 (66%)
 Frame = +2

Query: 284 RCKKIITLSGAGISTSAGIPDFRSPETGLYHNLQKYELPQPQAIFEINFFRQNPKPFFTL 463
           + K ++ L GAGISTS GI DFRS + G Y  L ++ L +P  +F+I+ FR+NP+ F+T 
Sbjct: 155 KAKNVVVLVGAGISTSLGILDFRS-DNGFYARLARHGLSEPSEMFDIHTFRENPEIFYTF 213

Query: 464 AKELFPGSFKPTISH 508
           A++L P +   + SH
Sbjct: 214 ARDLLPETNHYSPSH 228



 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 18/38 (47%), Positives = 26/38 (68%)
 Frame = +1

Query: 511 FIRLLHEKGLLLRHYTQNIDTLERGAGIPEEKLVEAHG 624
           FIRLL +K  L   +TQNID LE+  G+ + K+++ HG
Sbjct: 230 FIRLLEKKNKLSTLFTQNIDNLEKKTGLSDNKIIQCHG 267


>SPAC1783.04c |hst4||Sir2 family histone deacetylase
           Hst4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 415

 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 21/39 (53%), Positives = 31/39 (79%), Gaps = 1/39 (2%)
 Frame = +2

Query: 284 RCKKIITLSGAGISTSAGIPDFRSPETGLYHNLQ-KYEL 397
           + K+I+ ++GAGIS  AGIPDFRS E GL+ +L+ +Y+L
Sbjct: 56  KAKRIVVVTGAGISCDAGIPDFRSSE-GLFSSLRAEYKL 93



 Score = 29.1 bits (62), Expect = 0.64
 Identities = 14/27 (51%), Positives = 18/27 (66%)
 Frame = +1

Query: 499 NFTYFIRLLHEKGLLLRHYTQNIDTLE 579
           +F  F+  L ++  LLR YTQNID LE
Sbjct: 133 DFHLFLSQLAQESKLLRLYTQNIDFLE 159


>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 4924

 Score = 29.5 bits (63), Expect = 0.48
 Identities = 15/63 (23%), Positives = 33/63 (52%)
 Frame = +1

Query: 379  LTEVRTTSTTGDIRNKFLQAKSQTFLHISKGIISRKL*AYNFTYFIRLLHEKGLLLRHYT 558
            LT +      GD+ N+ LQ   + F +++KG   +    +NF+ F++   +  + L+ + 
Sbjct: 4237 LTSLDLARVDGDVANQLLQTIDKIFSNLTKGSFEKL--TFNFSNFVK-YRQYQINLKDFR 4293

Query: 559  QNI 567
            +N+
Sbjct: 4294 ENL 4296


>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 587

 Score = 29.5 bits (63), Expect = 0.48
 Identities = 12/27 (44%), Positives = 15/27 (55%)
 Frame = +3

Query: 36  ANSPPGKSGGHETVDESPQNVPPTTSM 116
           A +PPG  G + T + S  N PP  SM
Sbjct: 554 ATAPPGAPGSYNTSESSNLNAPPGVSM 580



 Score = 26.2 bits (55), Expect = 4.5
 Identities = 11/35 (31%), Positives = 16/35 (45%)
 Frame = +3

Query: 63  GHETVDESPQNVPPTTSMESLRNMFRDLDVDDVRM 167
           G    DE P ++PP      + N +RD    + RM
Sbjct: 18  GRRRFDEKPDSLPPLPDANGMSNGYRDSYKSNSRM 52


>SPCC962.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 536

 Score = 28.7 bits (61), Expect = 0.85
 Identities = 21/68 (30%), Positives = 37/68 (54%), Gaps = 4/68 (5%)
 Frame = +3

Query: 69  ETVDE--SPQNVPPTTSMESLRNMFRDLDVDDVRMYLALKLGLF--SPQDLEPAEPPEKV 236
           ET+D   S  N+P    + +LR M ++L+      +L + + LF  SP +L+P EP ++ 
Sbjct: 370 ETLDSKFSNWNLPHRLVLWTLRCM-KNLNNITATSFLLMHVSLFLGSPMNLQPVEPTQED 428

Query: 237 LDEVSLMV 260
           +  VS  +
Sbjct: 429 IASVSRFI 436


>SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase
            Rqh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1328

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 28/117 (23%), Positives = 47/117 (40%), Gaps = 2/117 (1%)
 Frame = +3

Query: 75   VDESPQNVPPTTSMESLRNMFRDLDVDDVRMYLALKLGLFSPQD--LEPAEPPEKVLDEV 248
            +DE  QN+  T    SL+++  D  +D   + L  + G     D   EP+ P E+  +EV
Sbjct: 1189 IDEKEQNLEGTELDPSLQSLDTDYPIDTNALSLDHEQGFSDDSDSVYEPSSPIEEGDEEV 1248

Query: 249  SLMVLSGGSRAIGARRS*HFQELVFLPLLEFQTSVVPKQDYIITYRSTNYLNHRRYS 419
                       +    S    +   +P  +  +   P + Y     ST+Y   R+YS
Sbjct: 1249 D----GQRKDILNFMNSQSLTQTGSVPKRKSTSYTRPSKSYRHKRGSTSYSRKRKYS 1301


>SPBC3B9.14c |mrpl3||mitochondrial ribosomal protein subunit
           L3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 326

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 11/34 (32%), Positives = 18/34 (52%), Gaps = 2/34 (5%)
 Frame = +1

Query: 22  YVKCRQIRLPEKV--AVMKPWMSHRRMSRQQLRW 117
           Y+KC+  RLPE+    +   WM  + +S+    W
Sbjct: 100 YIKCKYPRLPEEAVHGMQNGWMGSKALSQLATIW 133


>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 2280

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 18/52 (34%), Positives = 26/52 (50%)
 Frame = +2

Query: 299 ITLSGAGISTSAGIPDFRSPETGLYHNLQKYELPQPQAIFEINFFRQNPKPF 454
           + L  A +  + G+P  R P     H  + Y LP+     EI+FF QNP+ F
Sbjct: 407 VNLPAAQLQVAMGLPLSRIP-----HIRELYGLPR-DGDSEIDFFFQNPESF 452


>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 865

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 23/81 (28%), Positives = 39/81 (48%), Gaps = 1/81 (1%)
 Frame = +3

Query: 87  PQNVPPTTSMESLR-NMFRDLDVDDVRMYLALKLGLFSPQDLEPAEPPEKVLDEVSLMVL 263
           P+  P   + + L+ N   +  V +    L L +GL +P     AEP E  +DE++    
Sbjct: 625 PRTTPYQNAEQKLKANKHVEKRVQESNELLDL-IGLTTPS---VAEPLETPVDEMTQSPQ 680

Query: 264 SGGSRAIGARRS*HFQELVFL 326
           S  SRA    +  HF++++ L
Sbjct: 681 SSLSRAPSTSKKSHFEDILGL 701


>SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 704

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +3

Query: 84  SPQNVPPTTSMESLRNMFRDLDVDDVRMYLAL 179
           S  N  PT +     N+F DL V+++ +Y AL
Sbjct: 646 SSSNKRPTAAQLLTSNLFHDLVVNELHVYQAL 677


>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1323

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/28 (46%), Positives = 16/28 (57%), Gaps = 1/28 (3%)
 Frame = +2

Query: 338 IPDFRSPETGLYHNLQKYELPQP-QAIF 418
           +PDFR  + GL H  Q   LP+   AIF
Sbjct: 809 MPDFRGGDRGLSHKHQNIPLPKTCAAIF 836


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,174,143
Number of Sequences: 5004
Number of extensions: 70048
Number of successful extensions: 185
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)