Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021353
(698 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g55760.1 68418.m06950 transcriptional regulator Sir2 family p... 41 0.001
At5g09230.5 68418.m01052 transcriptional regulator Sir2 family p... 36 0.020
At5g09230.3 68418.m01049 transcriptional regulator Sir2 family p... 36 0.020
At5g09230.2 68418.m01051 transcriptional regulator Sir2 family p... 36 0.020
At5g09230.1 68418.m01050 transcriptional regulator Sir2 family p... 36 0.020
At5g67020.1 68418.m08449 expressed protein 30 1.7
At5g37750.1 68418.m04544 DNAJ heat shock N-terminal domain-conta... 29 2.2
At3g26410.1 68416.m03293 expressed protein 29 2.2
At1g65320.1 68414.m07407 CBS domain-containing protein contains ... 28 5.2
At1g65130.1 68414.m07384 ubiquitin carboxyl-terminal hydrolase-r... 28 5.2
At5g60710.1 68418.m07618 zinc finger (C3HC4-type RING finger) fa... 27 9.0
At5g44320.1 68418.m05427 eukaryotic translation initiation facto... 27 9.0
At3g50340.1 68416.m05505 expressed protein predicted protein, Ar... 27 9.0
At1g20950.1 68414.m02623 pyrophosphate--fructose-6-phosphate 1-p... 27 9.0
At1g05280.1 68414.m00534 fringe-related protein Similar to hypot... 27 9.0
>At5g55760.1 68418.m06950 transcriptional regulator Sir2 family
protein contains Pfam domain PF02146: transcriptional
regulator, Sir2 family
Length = 473
Score = 40.7 bits (91), Expect = 0.001
Identities = 31/86 (36%), Positives = 43/86 (50%), Gaps = 1/86 (1%)
Frame = +2
Query: 284 RCKKIITLSGAGISTSAGIPDFRSPETGLYHNLQKYELPQPQAIFEINFFRQNPKPFFTL 463
+ K ++ +GAGISTS GIPDFR P+ G++ LQ+ P+A + F R P
Sbjct: 43 KSKHLVVFTGAGISTSCGIPDFRGPK-GIW-TLQREGKDLPKA--SLPFHRAMPSMTHMA 98
Query: 464 AKEL-FPGSFKPTISHIS*DFYMRKG 538
EL G K IS ++R G
Sbjct: 99 LVELERAGILKFVISQNVDGLHLRSG 124
Score = 33.5 bits (73), Expect = 0.14
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = +1
Query: 523 LHEKGLLLRHYTQNIDTLERGAGIPEEKLVEAHG 624
L G+L +QN+D L +GIP EKL E HG
Sbjct: 102 LERAGILKFVISQNVDGLHLRSGIPREKLSELHG 135
>At5g09230.5 68418.m01052 transcriptional regulator Sir2 family
protein contains Pfam domain PF02146: transcriptional
regulator, Sir2 family;
Length = 354
Score = 36.3 bits (80), Expect = 0.020
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +2
Query: 281 DRCKKIITLSGAGISTSAGIPDFRSPETGLY 373
++ ++ L+GAG+ST GIPD+RSP G Y
Sbjct: 71 EQSSRLTILTGAGVSTECGIPDYRSP-NGAY 100
>At5g09230.3 68418.m01049 transcriptional regulator Sir2 family
protein contains Pfam domain PF02146: transcriptional
regulator, Sir2 family;
Length = 324
Score = 36.3 bits (80), Expect = 0.020
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +2
Query: 281 DRCKKIITLSGAGISTSAGIPDFRSPETGLY 373
++ ++ L+GAG+ST GIPD+RSP G Y
Sbjct: 90 EQSSRLTILTGAGVSTECGIPDYRSP-NGAY 119
>At5g09230.2 68418.m01051 transcriptional regulator Sir2 family
protein contains Pfam domain PF02146: transcriptional
regulator, Sir2 family;
Length = 373
Score = 36.3 bits (80), Expect = 0.020
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +2
Query: 281 DRCKKIITLSGAGISTSAGIPDFRSPETGLY 373
++ ++ L+GAG+ST GIPD+RSP G Y
Sbjct: 90 EQSSRLTILTGAGVSTECGIPDYRSP-NGAY 119
>At5g09230.1 68418.m01050 transcriptional regulator Sir2 family
protein contains Pfam domain PF02146: transcriptional
regulator, Sir2 family;
Length = 373
Score = 36.3 bits (80), Expect = 0.020
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +2
Query: 281 DRCKKIITLSGAGISTSAGIPDFRSPETGLY 373
++ ++ L+GAG+ST GIPD+RSP G Y
Sbjct: 90 EQSSRLTILTGAGVSTECGIPDYRSP-NGAY 119
>At5g67020.1 68418.m08449 expressed protein
Length = 394
Score = 29.9 bits (64), Expect = 1.7
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +2
Query: 314 AGISTSAGIPDFRSPETGLYHNLQKYELPQPQAIFEI 424
AG+S AG PD+RSP L H +LP F+I
Sbjct: 89 AGLSVGAGFPDWRSPGARL-HLRAMIDLPVAAVSFQI 124
>At5g37750.1 68418.m04544 DNAJ heat shock N-terminal
domain-containing protein contains Pfam profile PF00226
DnaJ domain
Length = 254
Score = 29.5 bits (63), Expect = 2.2
Identities = 17/63 (26%), Positives = 30/63 (47%)
Frame = +1
Query: 31 CRQIRLPEKVAVMKPWMSHRRMSRQQLRWKASEICFAISTWMTFECTWP*NSDSSALRIL 210
CRQI + ++ +P + ++ S QQ +W A C + + T + + A +IL
Sbjct: 187 CRQIFIATEITTKEPNKTTKKASHQQQQWSARSNCGSSANQSTKRGLDAQETVAPAEKIL 246
Query: 211 NRQ 219
RQ
Sbjct: 247 KRQ 249
>At3g26410.1 68416.m03293 expressed protein
Length = 477
Score = 29.5 bits (63), Expect = 2.2
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +2
Query: 338 IPDFRSPETGLYHNLQKYELPQPQAIFEINFFRQNPKPFFTLAKELF 478
+ D R P+ ++ N ++Y LP P A+ ++ N P+ + KE+F
Sbjct: 253 VRDGRGPDCNVWSNFKQYGLPMPVALLRMD---NNVPPWRSGLKEIF 296
>At1g65320.1 68414.m07407 CBS domain-containing protein contains
Pfam profile PF00571: CBS domain
Length = 425
Score = 28.3 bits (60), Expect = 5.2
Identities = 22/69 (31%), Positives = 32/69 (46%)
Frame = +3
Query: 3 RRNFVFLRKMSANSPPGKSGGHETVDESPQNVPPTTSMESLRNMFRDLDVDDVRMYLALK 182
R + ++ K NS S + D + + PTTSM S R+ F L +DV +L
Sbjct: 146 RFSILYNGKWLKNSENSSSSSGLSADSTNR---PTTSMTSSRDKFCCLSREDVIRFLIGV 202
Query: 183 LGLFSPQDL 209
LG +P L
Sbjct: 203 LGALAPLPL 211
>At1g65130.1 68414.m07384 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of
unknown function (DUF627)
Length = 1086
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +3
Query: 144 LDVDDVRMYLALKLGLFSPQDLEPAEPPEKVLDEVSLMVLSGGSR 278
L V D R Y+ + L+ ++LE A PPE + ++ GS+
Sbjct: 662 LSVFDYRSYILQPVKLYLEEELEDAAPPELLSEKKQEKAKKSGSK 706
>At5g60710.1 68418.m07618 zinc finger (C3HC4-type RING finger)
family protein low similarity to SP|P97280
Inter-alpha-trypsin inhibitor heavy chain H3 precursor
{Mesocricetus auratus}; contains Pfam profiles PF00097:
Zinc finger, C3HC4 type (RING finger), PF00092: von
Willebrand factor type A domain
Length = 704
Score = 27.5 bits (58), Expect = 9.0
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +3
Query: 162 RMYLALKLGLFSPQDLEPAEPPEKVLDEVSL 254
++ L L L L+ P+ LE + PP + D VSL
Sbjct: 9 KVALGLNLCLYVPKTLEDSSPPRRSDDAVSL 39
>At5g44320.1 68418.m05427 eukaryotic translation initiation factor 3
subunit 7, putative / eIF-3 zeta, putative / eIF3d,
putative similar to initiation factor 3d [Arabidopsis
thaliana] GI:12407755, SP|O15371 Eukaryotic translation
initiation factor 3 subunit 7 (eIF-3 zeta) (eIF3 p66)
(eIF3d) {Homo sapiens}; contains Pfam profile PF05091:
Eukaryotic translation initiation factor 3 subunit 7
(eIF-3)
Length = 588
Score = 27.5 bits (58), Expect = 9.0
Identities = 19/52 (36%), Positives = 24/52 (46%)
Frame = +3
Query: 54 KSGGHETVDESPQNVPPTTSMESLRNMFRDLDVDDVRMYLALKLGLFSPQDL 209
K+G ET DE NV S+ +R +DD MYL + L S DL
Sbjct: 349 KNGKRETFDEPIPNVNEGEENASIAYRYRRWKLDD-SMYLVARCELQSTVDL 399
>At3g50340.1 68416.m05505 expressed protein predicted protein,
Arabidopsis thaliana
Length = 403
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/37 (40%), Positives = 19/37 (51%)
Frame = +2
Query: 314 AGISTSAGIPDFRSPETGLYHNLQKYELPQPQAIFEI 424
AG+ AG PD+RSP L H +LP F+I
Sbjct: 89 AGLPVGAGFPDWRSPGARL-HLRAMIDLPIAAVSFQI 124
>At1g20950.1 68414.m02623 pyrophosphate--fructose-6-phosphate
1-phosphotransferase-related / pyrophosphate-dependent
6-phosphofructose-1-kinase-related similar to
pyrophosphate--fructose 6-phosphate 1-phosphotransferase
alpha subunit SP:Q41140 from [Ricinus communis]
Length = 614
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 6/51 (11%)
Frame = +1
Query: 400 STTGDIRNKFLQAK--SQTFLHISKGIISR----KL*AYNFTYFIRLLHEK 534
+T GD++N+F++A T ++ +IS L A + YFIRL+ K
Sbjct: 218 TTNGDLKNQFVEANVGFDTICKVNSQLISNACTDALSAEKYYYFIRLMGRK 268
>At1g05280.1 68414.m00534 fringe-related protein Similar to
hypothetical protein PID|e327464 (gb|Z97338) various
hypothetical proteins from Arabidopsis thaliana strong
similarity to unknown protein (pir||T13026) similarity
to predicted proteins + similar to hypothetical protein
GB:AAC23643 [Arabidopsis thaliana] + weak similarity to
Fringe [Schistocerca gregaria](GI:6573138);Fringe
encodes an extracellular protein that regulates Notch
signalling.
Length = 461
Score = 27.5 bits (58), Expect = 9.0
Identities = 22/81 (27%), Positives = 34/81 (41%), Gaps = 1/81 (1%)
Frame = +2
Query: 212 TGRTTRKSFG*SQPYGI-VRWIKSDRCKKIITLSGAGISTSAGIPDFRSPETGLYHNLQK 388
T R +KS G + Y R I D C++ +T +S+S+ +S Y N
Sbjct: 368 TFRPWQKSGGLASVYTFNTREIHRDPCQRPVTFYMQHVSSSSHDGTIKSVYKQAYENCTY 427
Query: 389 YELPQPQAIFEINFFRQNPKP 451
+ P+ I EI F + P
Sbjct: 428 DPVTSPRKIHEIRVFSRRLDP 448
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,471,287
Number of Sequences: 28952
Number of extensions: 362033
Number of successful extensions: 882
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 855
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 882
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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