Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021343
(661 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 155 4e-39
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 108 8e-25
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 108 8e-25
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 96 3e-21
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 78 1e-15
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 49 5e-07
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 40 4e-04
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 31 0.15
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.78
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 26 4.2
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 5.5
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 7.3
SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomy... 25 7.3
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 9.7
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 9.7
SPAC6F6.10c |arc2|arc34|ARP2/3 actin-organizing complex subunit ... 25 9.7
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 155 bits (377), Expect = 4e-39
Identities = 85/191 (44%), Positives = 118/191 (61%), Gaps = 1/191 (0%)
Frame = +1
Query: 91 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAITP-TT 267
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P T
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 268 QYSMPNVSSDVSSKMLLCKPT*STGLSRLSXXRQT*DQGSI*G*RQNLFPRGSQFHGAYE 447
+ + + + + K S ++ + + ++ G ++ P +
Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSK 157
Query: 448 NEGNCRSLSRQNCAECSYHGSAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 627
+ + + AYFND+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLD
Sbjct: 158 MKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLD 217
Query: 628 KKGTGERNVLI 660
K T E+++++
Sbjct: 218 KTDT-EKHIVV 227
Score = 64.1 bits (149), Expect = 2e-11
Identities = 28/48 (58%), Positives = 39/48 (81%)
Frame = +2
Query: 368 KPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 511
+P ++V G+ K F PEE+S+M+L+KMK+TAEAYLGK V +AV+TVP
Sbjct: 131 RPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVP 178
Score = 55.2 bits (127), Expect = 8e-09
Identities = 27/65 (41%), Positives = 38/65 (58%)
Frame = +3
Query: 255 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVXXEANLRSR*HIRVKTKPFSPRKSVP 434
NP NTIFD KRLIGRKF++ T+ D+K +PF +V + ++ K K F+P +
Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152
Query: 435 WCLRK 449
L K
Sbjct: 153 MILSK 157
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 108 bits (259), Expect = 8e-25
Identities = 49/58 (84%), Positives = 54/58 (93%)
Frame = +1
Query: 88 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAITP 261
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA+ P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
Score = 87.0 bits (206), Expect = 2e-18
Identities = 42/50 (84%), Positives = 44/50 (88%)
Frame = +1
Query: 511 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLI 660
AYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLI
Sbjct: 146 AYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLI 195
Score = 73.3 bits (172), Expect = 3e-14
Identities = 34/56 (60%), Positives = 44/56 (78%)
Frame = +2
Query: 344 FRGCQXGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 511
F+ + GKP I+V +KGE KTF PEE+SSMVL KM+E+AEA+LG V +AV+TVP
Sbjct: 90 FKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVP 145
Score = 71.7 bits (168), Expect = 9e-14
Identities = 33/68 (48%), Positives = 45/68 (66%)
Frame = +3
Query: 255 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVXXEANLRSR*HIRVKTKPFSPRKSVP 434
NP+NTIFDAKRLIGR+F D VQ+DMKHWPF+V+ + + + +TK F+P +
Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119
Query: 435 WCLRK*RK 458
L K R+
Sbjct: 120 MVLLKMRE 127
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 108 bits (259), Expect = 8e-25
Identities = 49/58 (84%), Positives = 54/58 (93%)
Frame = +1
Query: 88 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAITP 261
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA+ P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
Score = 90.2 bits (214), Expect = 2e-19
Identities = 43/50 (86%), Positives = 45/50 (90%)
Frame = +1
Query: 511 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLI 660
AYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLI
Sbjct: 146 AYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLI 195
Score = 76.6 bits (180), Expect = 3e-15
Identities = 36/56 (64%), Positives = 43/56 (76%)
Frame = +2
Query: 344 FRGCQXGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 511
F+ GKP ++V YKGE KTF PEE+SSMVL KM+ETAEAYLG V +AV+TVP
Sbjct: 90 FKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVP 145
Score = 73.3 bits (172), Expect = 3e-14
Identities = 34/68 (50%), Positives = 46/68 (67%)
Frame = +3
Query: 255 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVXXEANLRSR*HIRVKTKPFSPRKSVP 434
NP+NTIFDAKRLIGRKF+D VQ+DMKHWPF+V+ + + + +TK F+P +
Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119
Query: 435 WCLRK*RK 458
L K R+
Sbjct: 120 MVLMKMRE 127
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 96.3 bits (229), Expect = 3e-21
Identities = 43/58 (74%), Positives = 50/58 (86%)
Frame = +1
Query: 88 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAITP 261
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A+ P
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65
Score = 82.6 bits (195), Expect = 5e-17
Identities = 40/50 (80%), Positives = 43/50 (86%)
Frame = +1
Query: 511 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLI 660
AYF+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLI
Sbjct: 150 AYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLI 199
Score = 61.7 bits (143), Expect = 9e-11
Identities = 31/56 (55%), Positives = 38/56 (67%)
Frame = +2
Query: 344 FRGCQXGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 511
F+ G P I+V Y GE K F +E+S+MVLTKMKE +EA L K V+ AVITVP
Sbjct: 94 FKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVP 149
Score = 61.3 bits (142), Expect = 1e-10
Identities = 23/34 (67%), Positives = 29/34 (85%)
Frame = +3
Query: 255 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVV 356
NP NT+FDAKRLIGR++ED Q D+KHWPF+V+
Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVI 97
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 78.2 bits (184), Expect = 1e-15
Identities = 35/41 (85%), Positives = 39/41 (95%)
Frame = +1
Query: 511 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 633
AYFNDSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK
Sbjct: 192 AYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK 232
Score = 72.1 bits (169), Expect = 6e-14
Identities = 34/66 (51%), Positives = 44/66 (66%), Gaps = 1/66 (1%)
Frame = +1
Query: 79 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAI 255
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q I
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 256 TPTTQY 273
P +
Sbjct: 109 NPENTF 114
Score = 50.8 bits (116), Expect = 2e-07
Identities = 20/36 (55%), Positives = 28/36 (77%)
Frame = +2
Query: 404 KTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 511
KT+ P ++ +L+KM+ETA YLGK V+NAV+TVP
Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVP 191
Score = 44.8 bits (101), Expect = 1e-05
Identities = 18/34 (52%), Positives = 25/34 (73%)
Frame = +3
Query: 255 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVV 356
NP NT F KRLIGR+F++ VQ D+K P+++V
Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIV 142
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 49.2 bits (112), Expect = 5e-07
Identities = 20/59 (33%), Positives = 38/59 (64%)
Frame = +1
Query: 76 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 252
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61
Score = 46.0 bits (104), Expect = 5e-06
Identities = 20/40 (50%), Positives = 29/40 (72%)
Frame = +1
Query: 511 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDK 630
A+F D QR+A +A I+GLN LR++N+ AAA+ YG+ K
Sbjct: 150 AWFTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITK 189
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 39.5 bits (88), Expect = 4e-04
Identities = 16/53 (30%), Positives = 30/53 (56%)
Frame = +1
Query: 91 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQV 249
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQL 79
Score = 36.7 bits (81), Expect = 0.003
Identities = 17/56 (30%), Positives = 32/56 (57%)
Frame = +1
Query: 493 CSYHGSAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLI 660
C YF D+QR+A + A +GL VL++I++P A +A ++ ++ V++
Sbjct: 166 CVMSVPVYFTDAQRKALESAANEAGLPVLQLIHDPAAVILALMYSEEVLIDKTVVV 221
Score = 27.5 bits (58), Expect = 1.8
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +2
Query: 398 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 511
++K E S L ++ E+AE +LG V V++VP
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVP 171
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 31.1 bits (67), Expect = 0.15
Identities = 43/186 (23%), Positives = 73/186 (39%), Gaps = 6/186 (3%)
Frame = +1
Query: 91 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAITPTT 267
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83
Query: 268 QYSMPNVSS--DVS--SKMLLCKPT*STGLSRLSXXRQT*DQGSI*G*RQNLFPRGSQFH 435
+S+ NV D + +L+ K S +L +T S + +
Sbjct: 84 -HSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVISDEENYSLEEIIA 142
Query: 436 GAYENE-GNCRSLSRQNCAECSYHGSAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAI 612
E+ ++ + + +FN+ QR +A I +VL +I++ A AI
Sbjct: 143 MTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARILNKHVLALIDDNVAVAI 202
Query: 613 AYGLDK 630
Y L +
Sbjct: 203 EYSLSR 208
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.78
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 83 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 178
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 26.2 bits (55), Expect = 4.2
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +1
Query: 187 PSYVAFTDTERLIGDAAKNQVAITPTTQYSMPNVSSDVSSKMLLC 321
P +A T ER++ AAK A+ T Y++ ++ D K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.8 bits (54), Expect = 5.5
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = -1
Query: 169 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 11
G W SP S + H ++++ +F +RVL C+ L K +K+A L
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 25.4 bits (53), Expect = 7.3
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +1
Query: 436 GAYENEGNCRSLSRQNCAECSYHGSAYFND 525
GAY N C +S N A CS SA ND
Sbjct: 1689 GAYSNA--CVDISLSNLALCSILNSALIND 1716
>SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 654
Score = 25.4 bits (53), Expect = 7.3
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +3
Query: 228 RCRQEPGGDNPNNTIF 275
RCRQ+ DN N T+F
Sbjct: 397 RCRQQESKDNGNRTVF 412
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.0 bits (52), Expect = 9.7
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +1
Query: 199 AFTDTERLIGDAAKNQVAITPTTQYSMPNVSSDV 300
AF D + G AK A P+T+ + N+S V
Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188
>SPAC2F7.03c |pom1||DYRK family protein kinase
Pom1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1087
Score = 25.0 bits (52), Expect = 9.7
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = +3
Query: 69 QNGKSTRSRNRSGYHVLLRWCLPARE 146
+NGK T +R +S + W P++E
Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE 502
>SPAC6F6.10c |arc2|arc34|ARP2/3 actin-organizing complex subunit
Arc34|Schizosaccharomyces pombe|chr 1|||Manual
Length = 317
Score = 25.0 bits (52), Expect = 9.7
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -1
Query: 541 WLVFESH*STRNRDNCILHSFAEISFGSFLHF 446
+++FE H + +NR++CI H F + LHF
Sbjct: 243 FVLFERHFTPQNREDCISHIQV---FRNTLHF 271
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,891,584
Number of Sequences: 5004
Number of extensions: 62256
Number of successful extensions: 220
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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