BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021328 (699 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 134 9e-33 SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 113 2e-26 SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 97 2e-21 SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 95 1e-20 SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 92 8e-20 SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 90 3e-19 SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 78 1e-15 SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 73 3e-14 SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 38 0.001 SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 28 1.1 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 28 1.1 SPAC10F6.12c |mam4||protein-S isoprenylcysteine O-methyltransfer... 27 2.0 SPBC691.02c |||RINT1 family protein|Schizosaccharomyces pombe|ch... 26 4.5 SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 7.9 SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.9 SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co... 25 7.9 >SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit Cct4|Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 134 bits (325), Expect = 9e-33 Identities = 62/84 (73%), Positives = 75/84 (89%) Frame = +2 Query: 254 MDKMIQAANGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGAL 433 MDKMIQ GEV +TNDGATILK +SV+HPAAKMLV+LS AQD+EAGDGTTSVV++AG++ Sbjct: 44 MDKMIQTGKGEVILTNDGATILKHLSVLHPAAKMLVDLSAAQDVEAGDGTTSVVILAGSM 103 Query: 434 LDSAEKLLQKGIHPTVISDGFQKA 505 L AEKLL+KGIHPTVI++ FQ+A Sbjct: 104 LACAEKLLKKGIHPTVIAESFQRA 127 Score = 54.0 bits (124), Expect = 2e-08 Identities = 25/60 (41%), Positives = 42/60 (70%) Frame = +1 Query: 493 FPKSP*LALQVVENMSTPVDLNNEDALLKAAATSLNSKVVSQHSTILAPIAVQAIRAVWD 672 F ++ + ++ + ++L++ ++LL+AA TSLNSK+VSQ+S +LAPIAV A+ V D Sbjct: 124 FQRAAGFTVDCMKENALAIELSDRESLLRAATTSLNSKIVSQYSNLLAPIAVDAVLKVID 183 Score = 52.8 bits (121), Expect = 5e-08 Identities = 22/33 (66%), Positives = 30/33 (90%) Frame = +3 Query: 156 YKDKSKPTDIRLSNINAAKAVADAIRTSLGPRG 254 ++D+ KP ++RLSNI AA++VADAIRTSLGP+G Sbjct: 11 FQDREKPQEVRLSNIMAARSVADAIRTSLGPKG 43 >SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit Cct5|Schizosaccharomyces pombe|chr 1|||Manual Length = 546 Score = 113 bits (273), Expect = 2e-26 Identities = 52/84 (61%), Positives = 69/84 (82%) Frame = +2 Query: 254 MDKMIQAANGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGAL 433 +DK++ + +GE+T+TNDGATIL QM V H AK+LV+LS++QD E GDGTT VVV+AGAL Sbjct: 59 LDKILISPDGEITVTNDGATILDQMEVEHQIAKLLVQLSKSQDDEIGDGTTGVVVLAGAL 118 Query: 434 LDSAEKLLQKGIHPTVISDGFQKA 505 L+ AE L+ KGIHP I+DG++KA Sbjct: 119 LEQAEALIDKGIHPIRIADGYEKA 142 Score = 35.9 bits (79), Expect = 0.006 Identities = 24/63 (38%), Positives = 39/63 (61%), Gaps = 2/63 (3%) Frame = +1 Query: 493 FPKSP*LALQVVENMSTPVDLNNEDA--LLKAAATSLNSKVVSQHSTILAPIAVQAIRAV 666 + K+ +A++ ++ +S VD + E+ L ++A TSL SKVVS+ A IAV A+ +V Sbjct: 139 YEKACQVAVKHLDAISDVVDFSPENTTNLFRSAKTSLGSKVVSKAHDHFANIAVDAVLSV 198 Query: 667 WDL 675 DL Sbjct: 199 ADL 201 Score = 31.5 bits (68), Expect = 0.12 Identities = 14/21 (66%), Positives = 17/21 (80%) Frame = +3 Query: 192 SNINAAKAVADAIRTSLGPRG 254 S+I A K VA+ +RTSLGPRG Sbjct: 38 SHILATKTVANIVRTSLGPRG 58 >SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit Cct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 97.1 bits (231), Expect = 2e-21 Identities = 47/85 (55%), Positives = 62/85 (72%) Frame = +2 Query: 254 MDKMIQAANGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGAL 433 +DKM+ G+VT+TNDGATIL + V HPA K+LVEL++ QD E GDGTTSVV+IA L Sbjct: 46 LDKMLVDDIGDVTVTNDGATILSLLDVEHPAGKVLVELAQQQDKEVGDGTTSVVIIAAEL 105 Query: 434 LDSAEKLLQKGIHPTVISDGFQKAL 508 L A +L++ IHPT I G++ A+ Sbjct: 106 LRRANELVKNKIHPTTIITGYRLAI 130 Score = 29.9 bits (64), Expect = 0.37 Identities = 12/31 (38%), Positives = 20/31 (64%) Frame = +3 Query: 162 DKSKPTDIRLSNINAAKAVADAIRTSLGPRG 254 +K D+R N+ A A+A+ +++SLGP G Sbjct: 15 EKISGEDVRNQNVLATTAIANVVKSSLGPVG 45 >SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit Cct7|Schizosaccharomyces pombe|chr 2|||Manual Length = 558 Score = 94.7 bits (225), Expect = 1e-20 Identities = 44/83 (53%), Positives = 60/83 (72%) Frame = +2 Query: 257 DKMIQAANGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALL 436 DK++ GEV I+NDGATI+K + ++HPAAK LV+++RAQD E GDGTTSVVV AG LL Sbjct: 50 DKLMVDDRGEVVISNDGATIMKLLDIVHPAAKTLVDIARAQDAEVGDGTTSVVVFAGELL 109 Query: 437 DSAEKLLQKGIHPTVISDGFQKA 505 A ++ G+ +I G++KA Sbjct: 110 REARTFVEDGVSSHLIIRGYRKA 132 Score = 34.7 bits (76), Expect = 0.013 Identities = 18/31 (58%), Positives = 20/31 (64%) Frame = +3 Query: 162 DKSKPTDIRLSNINAAKAVADAIRTSLGPRG 254 D S+ LSNINA AV D IRT+LGP G Sbjct: 18 DDSQGRGQLLSNINACVAVQDTIRTTLGPLG 48 >SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit Cct3|Schizosaccharomyces pombe|chr 2|||Manual Length = 528 Score = 91.9 bits (218), Expect = 8e-20 Identities = 43/85 (50%), Positives = 60/85 (70%) Frame = +2 Query: 254 MDKMIQAANGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGAL 433 M KM+ G V +TNDG IL+++ V HPAAK ++EL+R QD E GDGTTSV+++AG + Sbjct: 44 MLKMLLDPVGSVLLTNDGHAILREIEVAHPAAKSMIELARTQDEEVGDGTTSVIILAGEI 103 Query: 434 LDSAEKLLQKGIHPTVISDGFQKAL 508 L +A LL + IHP V+ F++AL Sbjct: 104 LAAASPLLDRKIHPVVMIRSFKQAL 128 Score = 39.5 bits (88), Expect = 5e-04 Identities = 18/32 (56%), Positives = 23/32 (71%) Frame = +3 Query: 186 RLSNINAAKAVADAIRTSLGPRGWIR*FKQPM 281 ++SNI AAKAVAD IRT LGPR ++ P+ Sbjct: 21 QMSNIQAAKAVADVIRTCLGPRAMLKMLLDPV 52 Score = 34.7 bits (76), Expect = 0.013 Identities = 12/51 (23%), Positives = 34/51 (66%) Frame = +1 Query: 514 ALQVVENMSTPVDLNNEDALLKAAATSLNSKVVSQHSTILAPIAVQAIRAV 666 AL +++ ++ PV++++ + + T + +K+V++ S ++ +A++A+R V Sbjct: 131 ALSIIDEITLPVNVDDNAEMFRLIRTCIGTKLVARWSDLMCHLALRAVRTV 181 >SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit Cct2|Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 89.8 bits (213), Expect = 3e-19 Identities = 44/85 (51%), Positives = 63/85 (74%), Gaps = 1/85 (1%) Frame = +2 Query: 254 MDKMIQA-ANGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGA 430 MDK++Q+ ++G++ +TNDGATILK +++ + AAK+LV +S+ QD E GDGTTSV V A Sbjct: 45 MDKILQSNSSGDIVVTNDGATILKSIALDNAAAKVLVNISKVQDDEVGDGTTSVCVFAAE 104 Query: 431 LLDSAEKLLQKGIHPTVISDGFQKA 505 LL AE ++ IHP VI DG++ A Sbjct: 105 LLRQAEIMVNAKIHPQVIIDGYRIA 129 Score = 27.1 bits (57), Expect = 2.6 Identities = 11/23 (47%), Positives = 17/23 (73%) Frame = +3 Query: 186 RLSNINAAKAVADAIRTSLGPRG 254 RLS+ A AV D ++++LGP+G Sbjct: 22 RLSSFVGAIAVGDLVKSTLGPKG 44 >SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit Cct6|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 78.2 bits (184), Expect = 1e-15 Identities = 35/84 (41%), Positives = 53/84 (63%) Frame = +2 Query: 260 KMIQAANGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLD 439 KM+ G + +T DG +L +M + +P A + + + AQD GDGTTSV ++ G LL Sbjct: 43 KMLVDGAGAIKLTKDGKVLLTEMQIQNPTASCIAKAATAQDDATGDGTTSVCLLVGELLK 102 Query: 440 SAEKLLQKGIHPTVISDGFQKALN 511 AE +++G+HP++ISDGF A N Sbjct: 103 QAELYIREGLHPSLISDGFNLAKN 126 Score = 31.1 bits (67), Expect = 0.16 Identities = 18/51 (35%), Positives = 29/51 (56%) Frame = +1 Query: 514 ALQVVENMSTPVDLNNEDALLKAAATSLNSKVVSQHSTILAPIAVQAIRAV 666 AL +++ T +++ E LL A TSL++K+ S+ LAP V AI + Sbjct: 128 ALTFLDSFKTDFEVDRE-VLLNVAKTSLSTKISSKVVESLAPAVVDAILTI 177 >SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit Cct8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 546 Score = 73.3 bits (172), Expect = 3e-14 Identities = 32/73 (43%), Positives = 51/73 (69%) Frame = +2 Query: 293 ITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQKGIH 472 +TND ATI++++ VIHPAAK++V+ ++ Q+ E GD VVV G LL AE +++ G+ Sbjct: 64 LTNDAATIIRELEVIHPAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLT 123 Query: 473 PTVISDGFQKALN 511 P I+ G++ AL+ Sbjct: 124 PLEIAKGYEMALS 136 Score = 29.5 bits (63), Expect = 0.48 Identities = 16/50 (32%), Positives = 24/50 (48%) Frame = +3 Query: 105 IMAPKAGGDAIKANSSVYKDKSKPTDIRLSNINAAKAVADAIRTSLGPRG 254 + PKA G + Y+ D + N NA + +++ RTSLGP G Sbjct: 3 LRVPKASGPQLFREG--YRIMQGVEDAVIRNCNAIRELSEITRTSLGPNG 50 >SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60|Schizosaccharomyces pombe|chr 1|||Manual Length = 582 Score = 37.9 bits (84), Expect = 0.001 Identities = 22/87 (25%), Positives = 43/87 (49%), Gaps = 4/87 (4%) Frame = +2 Query: 263 MIQAANGEVTITNDGATILKQMSVIHP----AAKMLVELSRAQDIEAGDGTTSVVVIAGA 430 +I G IT DG T+ + +S+ A+++ +++ + AGDGTT+ V+ A Sbjct: 70 LIDQPFGSPKITKDGVTVARSVSLKDKFENLGARLVQDVASKTNEVAGDGTTTATVLTRA 129 Query: 431 LLDSAEKLLQKGIHPTVISDGFQKALN 511 + + + G +P + G Q A++ Sbjct: 130 IFSETVRNVAAGCNPMDLRRGIQLAVD 156 >SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizosaccharomyces pombe|chr 2|||Manual Length = 551 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/26 (53%), Positives = 14/26 (53%) Frame = +3 Query: 585 CNITKFQSSFTTLNYFGTHCSASYSS 662 CNI KF SSF T N F T SS Sbjct: 276 CNIPKFNSSFKTSNDFFTFTKTEESS 301 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 28.3 bits (60), Expect = 1.1 Identities = 27/92 (29%), Positives = 43/92 (46%), Gaps = 1/92 (1%) Frame = +2 Query: 230 PHKLRASWMDKMIQAANGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTS 409 P K+ W + ++AAN T++N K +S +L +L+ A TT Sbjct: 263 PEKILLRWFNYHLKAANWPRTVSN----FSKDVSDGENYTVLLNQLAPELCSRAPLQTTD 318 Query: 410 VVVIAGALLDSAEKL-LQKGIHPTVISDGFQK 502 V+ A +L +AEKL +K + PT + G K Sbjct: 319 VLQRAEQVLQNAEKLDCRKYLTPTAMVAGNPK 350 >SPAC10F6.12c |mam4||protein-S isoprenylcysteine O-methyltransferase Mam4|Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 27.5 bits (58), Expect = 2.0 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +1 Query: 328 ECYSSSCKNVGRIISCSRY*SW*WNNISSCYCWCVVGF 441 EC S K+ ++I+C R+ S+ N I S Y +GF Sbjct: 87 ECLLSGGKSFAKVINCLRFPSFLINFIFSVYQTSALGF 124 >SPBC691.02c |||RINT1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 678 Score = 26.2 bits (55), Expect = 4.5 Identities = 15/27 (55%), Positives = 15/27 (55%) Frame = +2 Query: 611 FHNTQLFWHPLQCKLFEQYGTYC*WCG 691 FH TQ PL LFEQ GTY W G Sbjct: 305 FHYTQ---DPLIIFLFEQNGTYDKWLG 328 >SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 722 Score = 25.4 bits (53), Expect = 7.9 Identities = 14/45 (31%), Positives = 19/45 (42%) Frame = -3 Query: 385 NILSTR*FYQHFCSWMNNTHLFQNCCSIISYCNFSIGCLNHLIHP 251 NI+ + S NNT + N C S N S+ L +HP Sbjct: 497 NIIKPNTYKNTILSNENNTPNYSNVCLSTSLINRSLPSLKSTMHP 541 >SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 542 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -3 Query: 448 LSRIQQRTSNNNY*CCSITSFNI 380 LS Q TS NN+ C SI +F I Sbjct: 177 LSNRQLNTSENNWTCLSIENFGI 199 >SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/39 (33%), Positives = 20/39 (51%), Gaps = 3/39 (7%) Frame = -3 Query: 529 RPPVKPI---KGFLETIRNYSWMYAFLKKFLSRIQQRTS 422 RPP K I L++ NY Y FL + +Q++T+ Sbjct: 496 RPPYKGIILMLNVLDSCTNYVGRYTFLNELFEYLQEKTT 534 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,716,336 Number of Sequences: 5004 Number of extensions: 52235 Number of successful extensions: 160 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 160 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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