Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021300
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g29900.1 68414.m03654 carbamoyl-phosphate synthase family pro... 87 8e-18
At1g03090.2 68414.m00284 methylcrotonyl-CoA carboxylase alpha ch... 32 0.40
At1g03090.1 68414.m00283 methylcrotonyl-CoA carboxylase alpha ch... 32 0.40
At2g25280.1 68415.m03024 expressed protein 30 1.2
At1g55350.4 68414.m06326 calpain-type cysteine protease family i... 30 1.6
At1g55350.3 68414.m06325 calpain-type cysteine protease family i... 30 1.6
At1g55350.2 68414.m06324 calpain-type cysteine protease family i... 30 1.6
At1g55350.1 68414.m06323 calpain-type cysteine protease family i... 30 1.6
At2g37690.1 68415.m04622 phosphoribosylaminoimidazole carboxylas... 29 2.8
At2g36580.1 68415.m04486 pyruvate kinase, putative similar to py... 29 3.8
At5g43950.1 68418.m05377 expressed protein 27 8.7
>At1g29900.1 68414.m03654 carbamoyl-phosphate synthase family protein
similar to carbamoylphosphate synthetase GI:6552726 from
[Medicago sativa]; contains Pfam profiles PF02786:
Carbamoyl-phosphate synthase L chain ATP binding domain,
PF00289: Carbamoyl-phosphate synthase L chain N-terminal
domain, PF02787: Carbamoyl-phosphate synthetase large
chain oligomerisation domain
Length = 1187
Score = 87.4 bits (207), Expect = 8e-18
Identities = 56/159 (35%), Positives = 86/159 (54%), Gaps = 3/159 (1%)
Frame = +3
Query: 42 AIGFCEEVGYPCLVRPSYVLSGAAMNVANSHQELETYLKSASQLSKEHPVVISKFIMDAK 221
A+ +EVGYP +VRPSYVL G AM + L TYL++A Q+ E PV++ K++ DA
Sbjct: 804 ALAIAKEVGYPVVVRPSYVLGGRAMEIVYDDSRLITYLENAVQVDPERPVLVDKYLSDAI 863
Query: 222 EIDVDVV--AADG*FIAWLFPNMLKTPVCTLETQP**PRHRILTTKL*KR*EISGIIAET 395
EIDVD + + I + ++ + V + ++ P I + L + +A+
Sbjct: 864 EIDVDTLTDSYGNVVIGGIMEHIEQAGVHSGDSACMLPTQTIPASCLQTIRTWTTKLAKK 923
Query: 396 LDVTGPFNMQ-LIAKDNDLKVIECNVRVSRSFPFVSKTL 509
L+V G N Q I D+ ++E N R SR+ PFVSK +
Sbjct: 924 LNVCGLMNCQYAITTSGDVFLLEANPRASRTVPFVSKAI 962
Score = 56.4 bits (130), Expect = 2e-08
Identities = 48/187 (25%), Positives = 85/187 (45%), Gaps = 6/187 (3%)
Frame = +3
Query: 27 TDLESAIGFCEEVG-YPCLVRPSYVLSGAAMNVANSHQELETYLKSASQLSKEHPVVISK 203
T L+ E++G +P ++RP++ L G +A + +E E+ KS S V++ K
Sbjct: 241 TTLDECFDIAEKIGEFPLIIRPAFTLGGTGGGIAYNKEEFESICKSGLAASATSQVLVEK 300
Query: 204 FIMDAKEIDVDVVA--ADG*FIAWLFPNMLKTPVCTLETQP**PRHRILTTKL*KR*EIS 377
++ KE +++V+ AD I N+ V T ++ P + + + + S
Sbjct: 301 SLLGWKEYELEVMRDLADNVVIICSIENIDPMGVHTGDSITVAPAQTLTDREYQRLRDYS 360
Query: 378 GIIAETLDVT-GPFNMQLIAK--DNDLKVIECNVRVSRSFPFVSKTLIMILWQWPLKLFW 548
I + V G N+Q D ++ +IE N RVSRS SK + + KL
Sbjct: 361 IAIIREIGVECGGSNVQFAVNPVDGEVMIIEMNPRVSRSSALASKATGFPIAKMAAKLSV 420
Query: 549 DYQLNRL 569
Y L+++
Sbjct: 421 GYTLDQI 427
Score = 37.9 bits (84), Expect = 0.006
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = +2
Query: 272 VSEHVENAGVHSGDATLVTPPQDINNETLEKIR 370
+ EH+E AGVHSGD+ + P Q I L+ IR
Sbjct: 882 IMEHIEQAGVHSGDSACMLPTQTIPASCLQTIR 914
Score = 30.3 bits (65), Expect = 1.2
Identities = 10/32 (31%), Positives = 21/32 (65%)
Frame = +2
Query: 278 EHVENAGVHSGDATLVTPPQDINNETLEKIRN 373
E+++ GVH+GD+ V P Q + + +++R+
Sbjct: 327 ENIDPMGVHTGDSITVAPAQTLTDREYQRLRD 358
>At1g03090.2 68414.m00284 methylcrotonyl-CoA carboxylase alpha
chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase
1 (MCCA) nearly identical to SP|Q42523
Methylcrotonyl-CoA carboxylase alpha chain,
mitochondrial precursor (EC 6.4.1.4)
(3-Methylcrotonyl-CoA carboxylase 1) (MCCase alpha
subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase
alpha subunit) {Arabidopsis thaliana}
Length = 734
Score = 31.9 bits (69), Expect = 0.40
Identities = 15/74 (20%), Positives = 40/74 (54%), Gaps = 4/74 (5%)
Frame = +3
Query: 30 DLESAIGFCEEVGYPCLVRPSYVLSGAAMNVANSHQEL-ETYL---KSASQLSKEHPVVI 197
D++ E++GYP +++P++ G M + S ++ +++L + A+ + +++
Sbjct: 176 DIDHMKSEAEKIGYPIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILL 235
Query: 198 SKFIMDAKEIDVDV 239
K+I + I+V +
Sbjct: 236 EKYITRPRHIEVQI 249
>At1g03090.1 68414.m00283 methylcrotonyl-CoA carboxylase alpha
chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase
1 (MCCA) nearly identical to SP|Q42523
Methylcrotonyl-CoA carboxylase alpha chain,
mitochondrial precursor (EC 6.4.1.4)
(3-Methylcrotonyl-CoA carboxylase 1) (MCCase alpha
subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase
alpha subunit) {Arabidopsis thaliana}
Length = 714
Score = 31.9 bits (69), Expect = 0.40
Identities = 15/74 (20%), Positives = 40/74 (54%), Gaps = 4/74 (5%)
Frame = +3
Query: 30 DLESAIGFCEEVGYPCLVRPSYVLSGAAMNVANSHQEL-ETYL---KSASQLSKEHPVVI 197
D++ E++GYP +++P++ G M + S ++ +++L + A+ + +++
Sbjct: 176 DIDHMKSEAEKIGYPIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILL 235
Query: 198 SKFIMDAKEIDVDV 239
K+I + I+V +
Sbjct: 236 EKYITRPRHIEVQI 249
>At2g25280.1 68415.m03024 expressed protein
Length = 291
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/43 (41%), Positives = 22/43 (51%)
Frame = -2
Query: 607 NFYSNLSTSRHHISRFNW*SQNNFSGHCHKIMINVLDTNGKDL 479
NF+S S H SRFN+ +N G HK I LD G D+
Sbjct: 178 NFFSVSSDFCHWGSRFNYMHYDNTHGAIHK-SIEALDKKGMDI 219
>At1g55350.4 68414.m06326 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 29.9 bits (64), Expect = 1.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 281 HVENAGVHSGDATLVTPPQDINNETLEKIRNIWNHS*NFRRNWS 412
HV ++G+ G A V ++++ L +IRN W + + WS
Sbjct: 1909 HVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWS 1952
>At1g55350.3 68414.m06325 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 29.9 bits (64), Expect = 1.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 281 HVENAGVHSGDATLVTPPQDINNETLEKIRNIWNHS*NFRRNWS 412
HV ++G+ G A V ++++ L +IRN W + + WS
Sbjct: 1909 HVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWS 1952
>At1g55350.2 68414.m06324 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 29.9 bits (64), Expect = 1.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 281 HVENAGVHSGDATLVTPPQDINNETLEKIRNIWNHS*NFRRNWS 412
HV ++G+ G A V ++++ L +IRN W + + WS
Sbjct: 1909 HVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWS 1952
>At1g55350.1 68414.m06323 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 29.9 bits (64), Expect = 1.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 281 HVENAGVHSGDATLVTPPQDINNETLEKIRNIWNHS*NFRRNWS 412
HV ++G+ G A V ++++ L +IRN W + + WS
Sbjct: 1909 HVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWS 1952
>At2g37690.1 68415.m04622 phosphoribosylaminoimidazole carboxylase,
putative / AIR carboxylase, putative similar to
SP|P55195 Phosphoribosylaminoimidazole carboxylase,
chloroplast precursor (EC 4.1.1.21) (AIR carboxylase)
(AIRC) {Vigna aconitifolia}; contains Pfam profiles
PF02222: ATP-grasp domain, PF00731: AIR carboxylase
Length = 642
Score = 29.1 bits (62), Expect = 2.8
Identities = 23/88 (26%), Positives = 41/88 (46%), Gaps = 2/88 (2%)
Frame = +3
Query: 12 KWKELTDLESAIGFCEEVGYPCLVRPS-YVLSGAAMNVANSHQELETYLKSASQLSKEHP 188
++ E++D+E A E GYP +++ G VAN+ EL + + + S+
Sbjct: 206 EFMEISDIEGARKAGELFGYPLMIKSKRLAYDGRGNAVANNQDELSSAVTALGGFSR--G 263
Query: 189 VVISKFIMDAKEIDVDVV-AADG*FIAW 269
+ I K+ KE+ V V DG + +
Sbjct: 264 LYIEKWAPFVKELAVIVARGRDGSMVCY 291
>At2g36580.1 68415.m04486 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Glycine max]
SWISS-PROT:Q42806
Length = 527
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 2/38 (5%)
Frame = +2
Query: 518 FVAMATKII--LGLPVEPANVMAGCGKVGVKVPQFSFS 625
F TKI+ LG V+AGC K G+ V +F FS
Sbjct: 25 FFPALTKIVGTLGPKSRSVEVIAGCLKAGMSVARFDFS 62
>At5g43950.1 68418.m05377 expressed protein
Length = 566
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -2
Query: 454 TFRSLSFAISCILNGPVTSKVSAM 383
TF L+F I C NGP T K+ M
Sbjct: 358 TFTDLAFWIFCPFNGPATLKLGLM 381
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,389,392
Number of Sequences: 28952
Number of extensions: 314535
Number of successful extensions: 742
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 742
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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