Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021295
(731 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R... 117 9e-27
At4g28470.1 68417.m04073 26S proteasome regulatory subunit, puta... 107 7e-24
At1g04810.1 68414.m00477 26S proteasome regulatory subunit, puta... 36 0.021
At4g17565.1 68417.m02626 F-box family protein contains F-box dom... 31 0.79
At2g18940.1 68415.m02211 pentatricopeptide (PPR) repeat-containi... 29 4.2
At5g35370.1 68418.m04204 lectin protein kinase family protein co... 28 5.6
At3g58930.1 68416.m06567 F-box family protein contains F-box dom... 28 5.6
At2g39870.1 68415.m04899 expressed protein 28 5.6
At1g58470.1 68414.m06651 RNA-binding protein (XF41) identical to... 28 7.3
At2g32730.1 68415.m04005 26S proteasome regulatory subunit, puta... 27 9.7
>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
(RPN1) contains an APC-complex (cyclosome) and
proteasome component repeat ( PS50248)
Length = 891
Score = 117 bits (281), Expect = 9e-27
Identities = 57/88 (64%), Positives = 67/88 (76%), Gaps = 6/88 (6%)
Frame = +1
Query: 10 VDSARQNLSATFVNAFVNAGFGRDKLVT------TDDGNKWMYKNKDHGMLSAAASLGMI 171
VDSARQNL+ATFVNAFVNAGFG+DKL+T T W++KNK+HG SAAASLGMI
Sbjct: 363 VDSARQNLAATFVNAFVNAGFGQDKLMTVPSDSTTGSSGNWLFKNKEHGKTSAAASLGMI 422
Query: 172 HLWDVDGGLTPIDKYLYTADEHIKAGAL 255
LWDVD GL+ +DKY ++ D I AGAL
Sbjct: 423 QLWDVDSGLSQLDKYFHSNDNPIIAGAL 450
Score = 107 bits (257), Expect = 7e-24
Identities = 44/84 (52%), Positives = 66/84 (78%)
Frame = +3
Query: 255 LALGLVNCGVRNECDPALALLSDYVLHSSSNLRIGSVLGLGIAYAGTQREDVLSHLLPVL 434
L +G+VNCG++N+CDPALALL DY+ S++RIG+++GLGI+YAG+Q + + + L P+L
Sbjct: 451 LGVGIVNCGIKNDCDPALALLGDYIDKEDSSVRIGAIMGLGISYAGSQNDQIRNKLSPIL 510
Query: 435 NDTTAPAEICALAAISCGLIAVGS 506
ND AP ++ A A++S G+I VGS
Sbjct: 511 NDAKAPLDVIAFASLSLGMIYVGS 534
Score = 52.4 bits (120), Expect = 3e-07
Identities = 29/74 (39%), Positives = 39/74 (52%)
Frame = +2
Query: 509 NGDVSCAIIQRLIDDNKDLHSSTYARFLHLGLGLCFLSCKERTEATMAALEVLPEPQQSL 688
N +V+ +II L+D ++ RFL LGLGL +L +E EAT + E +
Sbjct: 536 NEEVAQSIIFALMDRSEAELGDALTRFLPLGLGLLYLGKQESVEATAEVSKTFNEKIRKY 595
Query: 689 CQTTLSMCAYAGNG 730
C TL CAYAG G
Sbjct: 596 CDMTLLSCAYAGTG 609
Score = 33.9 bits (74), Expect = 0.11
Identities = 25/90 (27%), Positives = 38/90 (42%)
Frame = +3
Query: 237 HQGWCPLALGLVNCGVRNECDPALALLSDYVLHSSSNLRIGSVLGLGIAYAGTQREDVLS 416
HQG L L +V D + L + + N+R L LG+ + V+
Sbjct: 631 HQGPAVLGLAMVAMSEELGVDMEIRSLERMLQYGEQNIRRAVPLALGLLCISNPKVTVMD 690
Query: 417 HLLPVLNDTTAPAEICALAAISCGLIAVGS 506
L + +DT +E+ A IS GLI G+
Sbjct: 691 TLSRLSHDT--DSEVAMSAIISLGLIGAGT 718
>At4g28470.1 68417.m04073 26S proteasome regulatory subunit,
putative contains Pfam domain PF01851:
Proteasome/cyclosome repeat
Length = 1103
Score = 107 bits (257), Expect = 7e-24
Identities = 45/84 (53%), Positives = 65/84 (77%)
Frame = +3
Query: 255 LALGLVNCGVRNECDPALALLSDYVLHSSSNLRIGSVLGLGIAYAGTQREDVLSHLLPVL 434
L +G+VNCG++N+CDPA ALLS Y+ + S++RIG+++GLGIAYAG+Q + + L P+L
Sbjct: 494 LGVGIVNCGIKNDCDPAFALLSGYIDNEDSSVRIGAIMGLGIAYAGSQNDQIKIRLSPIL 553
Query: 435 NDTTAPAEICALAAISCGLIAVGS 506
ND AP ++ A AA+S G+I VGS
Sbjct: 554 NDANAPLDVIAFAALSLGMIYVGS 577
Score = 93.9 bits (223), Expect = 1e-19
Identities = 54/105 (51%), Positives = 65/105 (61%), Gaps = 23/105 (21%)
Frame = +1
Query: 10 VDSARQNLSATFVNAFVNAGFGRDKLV------TTDDGNKWMYKNKDHGMLSAAASL--- 162
VDSARQNLSATFVNAFVNAGFG+DKL+ T+ W++KNK+HG SA ASL
Sbjct: 389 VDSARQNLSATFVNAFVNAGFGQDKLMTVPSDSTSGSAGNWLFKNKEHGKTSAVASLVYL 448
Query: 163 --------------GMIHLWDVDGGLTPIDKYLYTADEHIKAGAL 255
GMI LWDV+ GL +DKY ++ D + AGAL
Sbjct: 449 ILLDKLISYVFILEGMIQLWDVETGLGHLDKYFHSNDNPVVAGAL 493
Score = 39.5 bits (88), Expect = 0.002
Identities = 29/87 (33%), Positives = 39/87 (44%), Gaps = 13/87 (14%)
Frame = +2
Query: 509 NGDVSCAIIQRLIDDNKDLHSSTYARFLHLGLGLCFLS-------------CKERTEATM 649
N +V+ +II L+D ++ RFL LGLGL +L +E EAT
Sbjct: 579 NEEVAQSIIFALMDRSEAELGEALTRFLPLGLGLLYLGKQVGSQSYLIIFLVRESVEATA 638
Query: 650 AALEVLPEPQQSLCQTTLSMCAYAGNG 730
+ E + C TL CAYAG G
Sbjct: 639 EVSKTFNEKIRKYCDMTLLSCAYAGTG 665
Score = 33.5 bits (73), Expect = 0.15
Identities = 24/90 (26%), Positives = 38/90 (42%)
Frame = +3
Query: 237 HQGWCPLALGLVNCGVRNECDPALALLSDYVLHSSSNLRIGSVLGLGIAYAGTQREDVLS 416
HQG + L +V D + L + + N+R L LG+ + V+
Sbjct: 703 HQGPAVIGLAMVAMSEELGLDMEIRSLERVLQYGEQNIRRAVPLALGLLCISNPKVTVMD 762
Query: 417 HLLPVLNDTTAPAEICALAAISCGLIAVGS 506
L + +DT +E+ A IS GLI G+
Sbjct: 763 TLSRLSHDT--DSEVAMAAIISLGLIGAGT 790
>At1g04810.1 68414.m00477 26S proteasome regulatory subunit,
putative contains similarity to 26S proteasome
regulatory subunit S1 SP:O88761, GI:3288594 from [Rattus
norvegicus]
Length = 1001
Score = 36.3 bits (80), Expect = 0.021
Identities = 30/90 (33%), Positives = 45/90 (50%), Gaps = 1/90 (1%)
Frame = +3
Query: 240 QGWCPLALGLVNCGVRNECDPALALLSDYVLHSSSN-LRIGSVLGLGIAYAGTQREDVLS 416
+G ALGL++ N + L D + +S ++ G+ LGLG+A GT ED+
Sbjct: 450 EGGALYALGLIHA---NHGEGIKQFLRDSLRSTSVEVIQHGACLGLGLAALGTADEDIYD 506
Query: 417 HLLPVLNDTTAPAEICALAAISCGLIAVGS 506
+ VL +A A A IS GL+ VG+
Sbjct: 507 DIKSVLYTDSAVAG--EAAGISMGLLLVGT 534
Score = 27.5 bits (58), Expect = 9.7
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Frame = +1
Query: 34 SAT-FVNAFVNAGFGRDKLVTTDDGNKWMYKNKDHGMLSAAASLGMIHLWDVDGGLTPID 210
SAT + NA ++AG D + + W+ + + SA A LG+IH + G + +
Sbjct: 376 SATIYANAIMHAGTTVDTFLR--ENLDWLSRATNWAKFSATAGLGVIHRGHLQQGRSLMA 433
Query: 211 KYL 219
YL
Sbjct: 434 PYL 436
>At4g17565.1 68417.m02626 F-box family protein contains F-box domain
Pfam:PF00646
Length = 378
Score = 31.1 bits (67), Expect = 0.79
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +1
Query: 115 MYKNKDHGMLSAAASLGMIHLWDVDGGLTPIDKYL 219
+Y +KDH + A S G I++WD + +DK+L
Sbjct: 203 IYNHKDHKVYQYA-SYGFIYVWDFSSDIPHVDKHL 236
>At2g18940.1 68415.m02211 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 822
Score = 28.7 bits (61), Expect = 4.2
Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 2/75 (2%)
Frame = -1
Query: 629 LCSLRNTARVPNAGTWRK*TSANPCYRQSIAE*LHKIHRHSGSNGDEPAGD--GGECADL 456
LC +++ PN TW T C + + + ++++ R S G EP D +
Sbjct: 444 LCDMKSNGCSPNRATWN--TMLALCGNKGMDKFVNRVFREMKSCGFEPDRDTFNTLISAY 501
Query: 455 GRGGRVVEHGKQVGQ 411
GR G V+ K G+
Sbjct: 502 GRCGSEVDASKMYGE 516
>At5g35370.1 68418.m04204 lectin protein kinase family protein
contains Pfam domains, PF01453: Lectin (probable mannose
binding) and PF00069: Protein kinase domain
Length = 870
Score = 28.3 bits (60), Expect = 5.6
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = +3
Query: 366 LGLGIAYAGTQREDVLSHLLPVLNDTTAPAEICALAAISCGLIAVGS*MAMYLVQ 530
LGLG++Y T D + H LP+L A +IC+ G+ + + YLV+
Sbjct: 346 LGLGVSYFSTHFTDPVEHGLPLL----ACHDICSKNCSCLGVFYENTSRSCYLVK 396
>At3g58930.1 68416.m06567 F-box family protein contains F-box domain
Pfam:PF00646
Length = 482
Score = 28.3 bits (60), Expect = 5.6
Identities = 17/74 (22%), Positives = 33/74 (44%)
Frame = -2
Query: 730 PVAGVRAHGQRGLAERLLRLRKHFQRGHRRLRALFAA*ETQPESQMQEPGVSRRVQILVI 551
PV V G RG R + + HF R + L+ + E + + + PG V+ ++
Sbjct: 399 PVKKVEIQGFRGTM-REINMIGHFLRSFKCLKEMGIFPEEEGPTNFENPGAFEYVEKILK 457
Query: 550 VNQSLNDCTRYIAI 509
+ +++C Y +
Sbjct: 458 LYNEISNCDVYFLV 471
>At2g39870.1 68415.m04899 expressed protein
Length = 330
Score = 28.3 bits (60), Expect = 5.6
Identities = 27/89 (30%), Positives = 41/89 (46%), Gaps = 4/89 (4%)
Frame = -2
Query: 688 ERLLRLRK---HFQRGHRRLRALFAA*ETQPESQMQEPGVSRRVQILVIVNQSLNDCTRY 518
E LLR + H + H+RL Q ++ E + RRV + + L + RY
Sbjct: 166 ESLLRRQNAAIHAELQHQRLIEQMWLCSAQSRFKLSENRIPRRV----VNEEGLFENPRY 221
Query: 517 IAIQDPTAMSPQEMAAS-AQISAGAVVSL 434
+ +PT + PQ+ AA + SAG V L
Sbjct: 222 VRRNNPTWLPPQQAAAPLKRPSAGTGVFL 250
>At1g58470.1 68414.m06651 RNA-binding protein (XF41) identical to
RNA binding protein GI:18181938 from (Arabidopsis
thaliana); contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
domain 15450911 gb AY054536.1
Length = 360
Score = 27.9 bits (59), Expect = 7.3
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +2
Query: 500 WILNGDVSCAIIQRLIDDNKDLHSSTYARFLHLGLGL 610
W + GD + A+I + +D D+HS + R +G+
Sbjct: 316 WPVTGDAAGALIHQFVDLKLDVHSQAHQRMNGGNMGI 352
>At2g32730.1 68415.m04005 26S proteasome regulatory subunit,
putative contains similarity to 26S proteasome
regulatory subunit S1 SP:O88761, GI:3288594 from [Rattus
norvegicus]
Length = 1004
Score = 27.5 bits (58), Expect = 9.7
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Frame = +1
Query: 34 SAT-FVNAFVNAGFGRDKLVTTDDGNKWMYKNKDHGMLSAAASLGMIHLWDVDGGLTPID 210
SAT + NA ++AG D + + W+ + + SA A LG+IH + G + +
Sbjct: 376 SATIYANAIMHAGTTVDTFLR--ENLDWLSRATNWAKFSATAGLGVIHRGHLQQGRSLMA 433
Query: 211 KYL 219
YL
Sbjct: 434 PYL 436
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,411,929
Number of Sequences: 28952
Number of extensions: 359814
Number of successful extensions: 1054
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1016
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1050
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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