BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021288 (708 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 177 1e-46 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 177 1e-46 AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 177 1e-46 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 173 1e-45 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 35 7e-04 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 8.7 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 177 bits (430), Expect = 1e-46 Identities = 83/90 (92%), Positives = 88/90 (97%) Frame = +2 Query: 254 QYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL 433 +YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL Sbjct: 11 KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL 70 Query: 434 GVKQLIVGVNKMDSTEPPYSEPRFEKSRRK 523 GVKQLIVGVNKMDSTEPPYSE RFE+ +++ Sbjct: 71 GVKQLIVGVNKMDSTEPPYSETRFEEIKKE 100 Score = 138 bits (335), Expect = 4e-35 Identities = 62/67 (92%), Positives = 64/67 (95%) Frame = +1 Query: 508 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 687 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCL Sbjct: 96 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCL 155 Query: 688 IEALDAI 708 IEALDAI Sbjct: 156 IEALDAI 162 Score = 22.2 bits (45), Expect = 5.0 Identities = 8/8 (100%), Positives = 8/8 (100%) Frame = +3 Query: 225 DIALWKFE 248 DIALWKFE Sbjct: 1 DIALWKFE 8 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 177 bits (430), Expect = 1e-46 Identities = 83/90 (92%), Positives = 88/90 (97%) Frame = +2 Query: 254 QYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL 433 +YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL Sbjct: 27 KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL 86 Query: 434 GVKQLIVGVNKMDSTEPPYSEPRFEKSRRK 523 GVKQLIVGVNKMDSTEPPYSE RFE+ +++ Sbjct: 87 GVKQLIVGVNKMDSTEPPYSETRFEEIKKE 116 Score = 138 bits (335), Expect = 4e-35 Identities = 62/67 (92%), Positives = 64/67 (95%) Frame = +1 Query: 508 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 687 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCL Sbjct: 112 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCL 171 Query: 688 IEALDAI 708 IEALDAI Sbjct: 172 IEALDAI 178 Score = 55.2 bits (127), Expect = 6e-10 Identities = 24/24 (100%), Positives = 24/24 (100%) Frame = +3 Query: 177 WVLDKLKAERERGITIDIALWKFE 248 WVLDKLKAERERGITIDIALWKFE Sbjct: 1 WVLDKLKAERERGITIDIALWKFE 24 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 177 bits (430), Expect = 1e-46 Identities = 83/90 (92%), Positives = 88/90 (97%) Frame = +2 Query: 254 QYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL 433 +YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL Sbjct: 84 KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL 143 Query: 434 GVKQLIVGVNKMDSTEPPYSEPRFEKSRRK 523 GVKQLIVGVNKMDSTEPPYSE RFE+ +++ Sbjct: 144 GVKQLIVGVNKMDSTEPPYSETRFEEIKKE 173 Score = 173 bits (421), Expect = 1e-45 Identities = 80/81 (98%), Positives = 80/81 (98%) Frame = +3 Query: 6 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 185 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 186 DKLKAERERGITIDIALWKFE 248 DKLKAERERGITIDIALWKFE Sbjct: 61 DKLKAERERGITIDIALWKFE 81 Score = 138 bits (335), Expect = 4e-35 Identities = 62/67 (92%), Positives = 64/67 (95%) Frame = +1 Query: 508 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 687 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCL Sbjct: 169 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCL 228 Query: 688 IEALDAI 708 IEALDAI Sbjct: 229 IEALDAI 235 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 173 bits (421), Expect = 1e-45 Identities = 80/81 (98%), Positives = 80/81 (98%) Frame = +3 Query: 6 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 185 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 186 DKLKAERERGITIDIALWKFE 248 DKLKAERERGITIDIALWKFE Sbjct: 61 DKLKAERERGITIDIALWKFE 81 Score = 171 bits (416), Expect = 6e-45 Identities = 80/90 (88%), Positives = 86/90 (95%) Frame = +2 Query: 254 QYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTL 433 +YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQTREHALLAFTL Sbjct: 84 KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTL 143 Query: 434 GVKQLIVGVNKMDSTEPPYSEPRFEKSRRK 523 GVKQLIVGVNKMD T+PPYSE RFE+ +++ Sbjct: 144 GVKQLIVGVNKMDMTDPPYSEARFEEIKKE 173 Score = 130 bits (315), Expect = 1e-32 Identities = 59/71 (83%), Positives = 64/71 (90%) Frame = +1 Query: 496 AQI*EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKAD 675 A+ EIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+VERK+G AD Sbjct: 165 ARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNAD 224 Query: 676 GKCLIEALDAI 708 GK LIEALDAI Sbjct: 225 GKTLIEALDAI 235 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 35.1 bits (77), Expect = 7e-04 Identities = 27/85 (31%), Positives = 39/85 (45%) Frame = +2 Query: 263 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 442 VT +D PGH FI G D VL+VAA G E QT + +A V Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246 Query: 443 QLIVGVNKMDSTEPPYSEPRFEKSR 517 +IV +NK+D + ++E ++ Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAK 271 Score = 25.4 bits (53), Expect = 0.53 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +3 Query: 18 KTHINIVVIGHVDSGKST 71 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.2 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +3 Query: 27 INIVVIGHVDSGKST 71 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 21.4 bits (43), Expect = 8.7 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -1 Query: 624 FG*RLQHVVSVPSRNGHESDSSWV 553 FG L H++ V +N + + WV Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 204,756 Number of Sequences: 438 Number of extensions: 4497 Number of successful extensions: 22 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 22 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 21804885 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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