Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021284
(469 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g13020.1 68418.m01492 emsy N terminus domain-containing prote... 33 0.096
At3g12140.2 68416.m01511 emsy N terminus domain-containing prote... 31 0.29
At3g12140.1 68416.m01510 emsy N terminus domain-containing prote... 31 0.29
At3g05270.1 68416.m00575 expressed protein similar to endosome-a... 31 0.39
At2g44440.1 68415.m05526 emsy N terminus domain-containing prote... 31 0.39
At1g24560.1 68414.m03090 expressed protein 31 0.39
At1g17070.1 68414.m02077 D111/G-patch domain-containing protein ... 31 0.39
At5g41790.1 68418.m05088 COP1-interactive protein 1 / CIP1 almos... 29 1.2
At5g04990.1 68418.m00528 sad1/unc-84 protein-related contains we... 29 1.2
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 29 1.2
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 29 1.2
At2g46180.1 68415.m05742 intracellular protein transport protein... 29 2.1
At3g05420.2 68416.m00594 acyl-CoA binding family protein similar... 28 2.7
At3g05420.1 68416.m00593 acyl-CoA binding family protein similar... 28 2.7
At1g10220.1 68414.m01152 hypothetical protein 28 2.7
At5g61560.1 68418.m07725 protein kinase family protein contains ... 28 3.6
At5g27030.1 68418.m03224 WD-40 repeat family protein contains 8 ... 27 4.8
At2g44410.1 68415.m05523 expressed protein 27 4.8
At1g66840.1 68414.m07597 expressed protein contains Pfam profile... 27 4.8
At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 27 8.4
At5g56360.1 68418.m07034 calmodulin-binding protein similar to a... 27 8.4
At5g47820.2 68418.m05908 kinesin-like protein (FRA1) identical t... 27 8.4
At5g47820.1 68418.m05907 kinesin-like protein (FRA1) identical t... 27 8.4
At4g27595.1 68417.m03964 protein transport protein-related low s... 27 8.4
At4g27190.1 68417.m03905 disease resistance protein (NBS-LRR cla... 27 8.4
At4g17210.1 68417.m02588 myosin heavy chain-related contains wea... 27 8.4
At3g01015.1 68416.m00001 expressed protein ; expression supporte... 27 8.4
>At5g13020.1 68418.m01492 emsy N terminus domain-containing protein
/ ENT domain-containing protein contains Pfam profile
PF03735: ENT domain
Length = 397
Score = 33.1 bits (72), Expect = 0.096
Identities = 18/60 (30%), Positives = 35/60 (58%)
Frame = +1
Query: 265 DTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNSD 444
+T++ + E Y S A + + EKE+++T+LRK R++ E ++L + R+N+D
Sbjct: 51 ETQIHLIEQEAYSSILRAFKAQSDAITWEKESLITELRKELRVSDEEHREL--LSRVNAD 108
>At3g12140.2 68416.m01511 emsy N terminus domain-containing protein
/ ENT domain-containing protein contains Pfam profile
PF03735: ENT domain
Length = 327
Score = 31.5 bits (68), Expect = 0.29
Identities = 16/60 (26%), Positives = 36/60 (60%)
Frame = +1
Query: 265 DTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNSD 444
+T++ + + E Y + A + ++ EKE+++T+LRK R++ + ++L + R+N D
Sbjct: 2 ETQIHQLEQEAYTAVLRAFKAQSDAISWEKESLITELRKELRVSDDEHREL--LSRVNKD 59
>At3g12140.1 68416.m01510 emsy N terminus domain-containing protein
/ ENT domain-containing protein contains Pfam profile
PF03735: ENT domain
Length = 327
Score = 31.5 bits (68), Expect = 0.29
Identities = 16/60 (26%), Positives = 36/60 (60%)
Frame = +1
Query: 265 DTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNSD 444
+T++ + + E Y + A + ++ EKE+++T+LRK R++ + ++L + R+N D
Sbjct: 2 ETQIHQLEQEAYTAVLRAFKAQSDAISWEKESLITELRKELRVSDDEHREL--LSRVNKD 59
>At3g05270.1 68416.m00575 expressed protein similar to
endosome-associated protein (EEA1) (GI:1016368) [Homo
sapiens]; similar to smooth muscle myosin heavy chain
(GI:4417214) [Homo sapiens; contains Pfam profile
PF05911: Plant protein of unknown function (DUF869)
Length = 615
Score = 31.1 bits (67), Expect = 0.39
Identities = 19/65 (29%), Positives = 34/65 (52%)
Frame = +1
Query: 262 EDTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNS 441
E E+ K QLE+ + + + E+ Q E E L++++K + NQEL L E +
Sbjct: 363 EMVEVEKLQLEMALNGSKEQIEALQSRLKEIEGKLSEMKKLEAENQELELLLGESGKQME 422
Query: 442 DVYQR 456
D+ ++
Sbjct: 423 DLQRQ 427
>At2g44440.1 68415.m05526 emsy N terminus domain-containing protein
/ ENT domain-containing protein contains Pfam profile
PF03735: ENT domain
Length = 429
Score = 31.1 bits (67), Expect = 0.39
Identities = 18/60 (30%), Positives = 35/60 (58%)
Frame = +1
Query: 265 DTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNSD 444
+ ++ + + E Y S A + ++ EKE+V+T+LRK ++ E ++L + R+NSD
Sbjct: 58 EAQIHQIEKEAYISVLRAFKAQGDAISWEKESVITELRKELSLSNEEHREL--LGRVNSD 115
>At1g24560.1 68414.m03090 expressed protein
Length = 678
Score = 31.1 bits (67), Expect = 0.39
Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +1
Query: 277 LKA-QLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRR 432
LKA +E K E++RE R E EKE + +L + E++++LDE R
Sbjct: 68 LKALAVEAIKKRDESKRE-RDEALKEKENLTNELENVNKGKDEMSKKLDEALR 119
>At1g17070.1 68414.m02077 D111/G-patch domain-containing protein
Similar to SP|Q9ERA6 Tuftelin-interacting protein 11
{Mus musculus}; contains Pfam profile PF01585: G-patch
domain
Length = 849
Score = 31.1 bits (67), Expect = 0.39
Identities = 14/55 (25%), Positives = 29/55 (52%)
Frame = +1
Query: 271 ELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRL 435
+L++ +++ D ERES + EKE ++ + K +R + + DE+ R+
Sbjct: 355 DLVEHEIQKIDRDLRNERESALSLQQEKEMLINEEEKQKRHLENMEYIADEISRI 409
>At5g41790.1 68418.m05088 COP1-interactive protein 1 / CIP1 almost
identical to CIP1 (GI:836950) [Arabidopsis thaliana]
Length = 1305
Score = 29.5 bits (63), Expect = 1.2
Identities = 15/59 (25%), Positives = 31/59 (52%)
Frame = +1
Query: 289 LELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNSDVYQRATA 465
L++ KS+ EAE E ++ SE +TD++K + L+E + +++++ A
Sbjct: 996 LQVQKSETEAELEREKQEKSELSNQITDVQKALVEQEAAYNTLEEEHKQINELFKETEA 1054
Score = 27.1 bits (57), Expect = 6.3
Identities = 19/47 (40%), Positives = 25/47 (53%)
Frame = +1
Query: 289 LELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVR 429
LEL +S AE ESR ET +L +TQ M QELT +++
Sbjct: 678 LELSESLKAAEEESRTMSTKISETS-DELERTQIMVQELTADSSKLK 723
>At5g04990.1 68418.m00528 sad1/unc-84 protein-related contains weak
similarity to Sad1/unc-84 protein-like 1
(Swiss-Prot:O94901) [Homo sapiens]
Length = 471
Score = 29.5 bits (63), Expect = 1.2
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +1
Query: 259 KEDTELLKAQLELYKSDFEAERES-RQEMASEKETVLTDLRKTQRMNQELTQQLDEV 426
K T ++ Q+EL E E + RQE+ + ++L+K + + L + +DEV
Sbjct: 166 KATTNSMQVQVELLDKKMEREAKVLRQEIERKASAFQSELKKIESRTESLEKSVDEV 222
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 29.5 bits (63), Expect = 1.2
Identities = 12/50 (24%), Positives = 28/50 (56%)
Frame = +1
Query: 274 LLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDE 423
+L+ ++ + K + E +R R+E+ E T+ + + ++E+ + LDE
Sbjct: 2267 VLENKVNVVKDEAERQRLQREELEMELHTIRQQMESARNADEEMKRILDE 2316
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 29.5 bits (63), Expect = 1.2
Identities = 16/58 (27%), Positives = 32/58 (55%)
Frame = +1
Query: 259 KEDTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRR 432
+E+ E + + E K + EAE+ ++E EKE + R+ +R +E ++++ RR
Sbjct: 494 REEEEAKRREEERKKREEEAEQARKREEEREKEEEMAKKREEERQRKE-REEVERKRR 550
>At2g46180.1 68415.m05742 intracellular protein transport protein
USO1-related similar to Intracellular protein transport
protein USO1 (Swiss-Prot:P25386) [Saccharomyces
cerevisiae]
Length = 725
Score = 28.7 bits (61), Expect = 2.1
Identities = 20/69 (28%), Positives = 42/69 (60%), Gaps = 2/69 (2%)
Frame = +1
Query: 259 KEDTELLKAQLELYKSDFEAERE-SRQEMASEKETVLT-DLRKTQRMNQELTQQLDEVRR 432
KED E +LE + E++ +RQE+ K+ +L + ++++M+++ ++ +DE+R+
Sbjct: 346 KEDMEKSLQRLEKELEEARREKDKARQELKRLKQHLLEKETEESEKMDED-SRLIDELRQ 404
Query: 433 LNSDVYQRA 459
N YQR+
Sbjct: 405 TNE--YQRS 411
>At3g05420.2 68416.m00594 acyl-CoA binding family protein similar to
PIR|S68824|S68824 rngB protein, cytosolic (Dictyostelium
discoideum); contains Pfam profiles PF00887: Acyl CoA
binding protein, PF01344: Kelch motif
Length = 669
Score = 28.3 bits (60), Expect = 2.7
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 346 SEKETVLTDLRKTQRMNQELTQQLDEVRRLNSDVYQ 453
SEKE + L K + +L Q+L E N+D+Y+
Sbjct: 553 SEKEELEASLNKERMQTLQLRQELGEAELRNTDLYK 588
>At3g05420.1 68416.m00593 acyl-CoA binding family protein similar to
PIR|S68824|S68824 rngB protein, cytosolic (Dictyostelium
discoideum); contains Pfam profiles PF00887: Acyl CoA
binding protein, PF01344: Kelch motif
Length = 668
Score = 28.3 bits (60), Expect = 2.7
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 346 SEKETVLTDLRKTQRMNQELTQQLDEVRRLNSDVYQ 453
SEKE + L K + +L Q+L E N+D+Y+
Sbjct: 552 SEKEELEASLNKERMQTLQLRQELGEAELRNTDLYK 587
>At1g10220.1 68414.m01152 hypothetical protein
Length = 267
Score = 28.3 bits (60), Expect = 2.7
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +1
Query: 307 DFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLN 438
DF + ES+ E EKE TDL Q+ + L + L ++ R N
Sbjct: 108 DFNVKTESKTEQ--EKEIKQTDLETEQKQSCVLNENLKKISRPN 149
>At5g61560.1 68418.m07725 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 796
Score = 27.9 bits (59), Expect = 3.6
Identities = 20/74 (27%), Positives = 37/74 (50%)
Frame = +2
Query: 35 LEKQHSAVLSNIGQVRGTLSIFEGIFKDCSNILDSKAKEAKNPGTPNIEMLTSALVARGQ 214
L+ S +L+ G R + S D S+ S+ +EA + T + +S+ + +
Sbjct: 238 LDVDESKLLNQKGFYRTSSSGIGYGGSDISSWRSSQMEEASSSSTYSDPTSSSSQIHKDF 297
Query: 215 ELQMLKVEIDKLKG 256
EL+ LK+E+ +KG
Sbjct: 298 ELEKLKIELRHIKG 311
>At5g27030.1 68418.m03224 WD-40 repeat family protein contains 8
WD-40 repeats (PF00400) (2 weak)
Length = 1108
Score = 27.5 bits (58), Expect = 4.8
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = +1
Query: 277 LKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNS 441
+K E+ K + E RQE A E ++ DLR N+EL +++ ++ L +
Sbjct: 70 MKIFFEIRKQKY-LEALDRQEKAKAVEILVQDLRVFSTFNEELYKEITQLLTLQN 123
>At2g44410.1 68415.m05523 expressed protein
Length = 413
Score = 27.5 bits (58), Expect = 4.8
Identities = 22/59 (37%), Positives = 28/59 (47%)
Frame = +3
Query: 279 ESTTRTVQERLRSGTRVSSRDGQRKGDRAHGPQENSTNEPRADATAGRGTEAELGRLPE 455
ES +QER+R + SR +R+ P S NE R D+TAG E RL E
Sbjct: 40 ESAQERIQERIRQLEVIVSRIREREITTTTTPALVSPNEHR-DSTAGVIHERSRERLVE 97
>At1g66840.1 68414.m07597 expressed protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827);
expression supported by MPSS
Length = 607
Score = 27.5 bits (58), Expect = 4.8
Identities = 20/53 (37%), Positives = 31/53 (58%), Gaps = 3/53 (5%)
Frame = +1
Query: 292 ELYKSDFEAE--RESRQEMASEKETVLTDLRKTQRMNQELTQQLDEV-RRLNS 441
+L+KS E RESR+ S K +L K +++ +ELT +++E RRL S
Sbjct: 35 DLHKSGRELGIYRESRRVAESAKAKAEVELCKAKKIVKELTLRIEESNRRLKS 87
Score = 27.1 bits (57), Expect = 6.3
Identities = 15/56 (26%), Positives = 28/56 (50%), Gaps = 4/56 (7%)
Frame = +1
Query: 277 LKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQR----MNQELTQQLDEVRR 432
L E KSD EA ++ ++ E + +++KT+ +EL +LDE+ +
Sbjct: 361 LTTSFEKLKSDREAAKKEELKLREEARIINNEIQKTETGFDGKEKELLSKLDELEK 416
>At5g67270.1 68418.m08480 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profiles PF00307: Calponin homology (CH)
domain, PF03271: EB1 protein
Length = 329
Score = 26.6 bits (56), Expect = 8.4
Identities = 17/49 (34%), Positives = 20/49 (40%), Gaps = 3/49 (6%)
Frame = +3
Query: 252 RGERGHGAFESTTR---TVQERLRSGTRVSSRDGQRKGDRAHGPQENST 389
R E G E+T R T Q S + R G R H PQ N+T
Sbjct: 129 RREASKGGKEATKRAAATQQSGKSSSSSAPPRPSSSNGTRKHEPQSNNT 177
>At5g56360.1 68418.m07034 calmodulin-binding protein similar to
alpha glucosidase II beta subunit from GI:2104691 [Mus
musculus]
Length = 647
Score = 26.6 bits (56), Expect = 8.4
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = +2
Query: 119 CSNILDSKAKEAKNPGTPNIEMLTSALVARGQELQMLKVEIDK 247
C N K A+ IE LV R QE++ KV ++K
Sbjct: 129 CQNTCWEAGKAARENLKKKIETYNQGLVIRRQEIEQAKVGLEK 171
>At5g47820.2 68418.m05908 kinesin-like protein (FRA1) identical to
kinesin-like protein [Arabidopsis thaliana] GI:27260890;
contains Pfam profile PF00225: Kinesin motor domain
Length = 1035
Score = 26.6 bits (56), Expect = 8.4
Identities = 16/68 (23%), Positives = 32/68 (47%)
Frame = +1
Query: 259 KEDTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLN 438
+++ + +KAQ + + E E ++ + +E L LRK R ++ +L + +
Sbjct: 620 QDEIQSIKAQKVQLQHRMKQEAEQFRQWKASREKELLQLRKEGRKSEYERHKLQALNQRQ 679
Query: 439 SDVYQRAT 462
V QR T
Sbjct: 680 KMVLQRKT 687
>At5g47820.1 68418.m05907 kinesin-like protein (FRA1) identical to
kinesin-like protein [Arabidopsis thaliana] GI:27260890;
contains Pfam profile PF00225: Kinesin motor domain
Length = 1035
Score = 26.6 bits (56), Expect = 8.4
Identities = 16/68 (23%), Positives = 32/68 (47%)
Frame = +1
Query: 259 KEDTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLN 438
+++ + +KAQ + + E E ++ + +E L LRK R ++ +L + +
Sbjct: 620 QDEIQSIKAQKVQLQHRMKQEAEQFRQWKASREKELLQLRKEGRKSEYERHKLQALNQRQ 679
Query: 439 SDVYQRAT 462
V QR T
Sbjct: 680 KMVLQRKT 687
>At4g27595.1 68417.m03964 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 1212
Score = 26.6 bits (56), Expect = 8.4
Identities = 13/58 (22%), Positives = 27/58 (46%)
Frame = +1
Query: 259 KEDTELLKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRR 432
KE+T L+ +E KSD E + + ++T + ++ EL +L+ ++
Sbjct: 390 KEETSKLEKLVESIKSDLETTQGEKVRALLNEKTATSQIQNLLSEKTELATELENCKK 447
>At4g27190.1 68417.m03905 disease resistance protein (NBS-LRR
class), putative domain signature NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 985
Score = 26.6 bits (56), Expect = 8.4
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +1
Query: 286 QLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNSD 444
+L+L + EAE + +E V + RM+++L + LDEV+ L D
Sbjct: 65 RLKLMRWQREAEEVISKARLKLEERVSCGMSLRPRMSRKLVKILDEVKMLEKD 117
>At4g17210.1 68417.m02588 myosin heavy chain-related contains weak
similarity to Swiss-Prot:P14105 myosin heavy chain,
nonmuscle (Cellular myosin heavy chain) (NMMHC) [Gallus
gallus]
Length = 527
Score = 26.6 bits (56), Expect = 8.4
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +1
Query: 313 EAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRR 432
EAE E + + + ++ + KT++ +E+ +DE+RR
Sbjct: 343 EAEEGEWVEASRKVDEIMREAEKTRKEAEEMRMNVDELRR 382
>At3g01015.1 68416.m00001 expressed protein ; expression supported
by MPSS
Length = 488
Score = 26.6 bits (56), Expect = 8.4
Identities = 16/61 (26%), Positives = 29/61 (47%)
Frame = +1
Query: 277 LKAQLELYKSDFEAERESRQEMASEKETVLTDLRKTQRMNQELTQQLDEVRRLNSDVYQR 456
+ L+L+ ER +EK L + KT+R Q+ + +E+RRL ++ +
Sbjct: 370 IPVDLKLHSDIRAVERAEFDYQVTEKIN-LVEQYKTERERQQKLAEEEEIRRLRKELVPK 428
Query: 457 A 459
A
Sbjct: 429 A 429
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,900,851
Number of Sequences: 28952
Number of extensions: 143454
Number of successful extensions: 667
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 649
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 791932800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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