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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021283
         (749 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protei...    81   6e-16
At1g64410.1 68414.m07301 hypothetical protein                          31   1.1  
At1g52960.1 68414.m05990 hypothetical protein very low similarit...    31   1.1  
At4g36980.1 68417.m05240 expressed protein                             29   2.5  
At2g33060.1 68415.m04054 leucine-rich repeat family protein cont...    29   2.5  
At5g61100.1 68418.m07666 hypothetical protein                          29   3.3  
At5g17980.1 68418.m02109 C2 domain-containing protein contains I...    29   4.4  
At1g11780.1 68414.m01352 oxidoreductase, 2OG-Fe(II) oxygenase fa...    29   4.4  
At1g04840.1 68414.m00480 pentatricopeptide (PPR) repeat-containi...    29   4.4  
At3g19670.1 68416.m02492 FF domain-containing protein / WW domai...    28   7.6  
At1g22240.1 68414.m02780 pumilio/Puf RNA-binding domain-containi...    28   7.6  

>At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protein
           similar to SP|P12048 Bifunctional purine biosynthesis
           protein purH [Includes:
           Phosphoribosylaminoimidazolecarboxamide
           formyltransferase (EC 2.1.2.3) (AICAR transformylase);
           IMP cyclohydrolase (EC 3.5.4.10) (Inosinicase) (IMP
           synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam
           profiles PF01808: AICARFT/IMPCHase bienzyme, PF02142:
           MGS-like domain
          Length = 596

 Score = 81.4 bits (192), Expect = 6e-16
 Identities = 40/84 (47%), Positives = 52/84 (61%), Gaps = 1/84 (1%)
 Frame = +1

Query: 4   ECGLQLIASGGTARSLRNAGLTVQDVSDITRAPEMLGGRVKTLHPAVHAGILARLS-DSD 180
           E G  ++++GGTA +L NAG++V  V  +T  PEML GRVKTLHP +H GILAR   +  
Sbjct: 93  ELGYTIVSTGGTASTLENAGVSVTKVEKLTHFPEMLDGRVKTLHPNIHGGILARRDVEHH 152

Query: 181 QEDMKRQKYEMISVVVCNLYPFVQ 252
            E +         VVV NLYPF +
Sbjct: 153 MEALNEHGIGTFDVVVVNLYPFYE 176



 Score = 50.4 bits (115), Expect = 1e-06
 Identities = 31/84 (36%), Positives = 45/84 (53%)
 Frame = +2

Query: 332 AKNHDRVTVVCDPADYDAVVKEIKENKHHQTTLGTRQRLALKAFTHTSDYDLAISDYFRS 511
           AKNH  V +V D  DY AV++ +K  +  Q     R++LA KAF H + YD A+S++   
Sbjct: 205 AKNHKDVLIVVDSGDYQAVLEYLKGGQSDQQ---FRRKLAWKAFQHVAAYDSAVSEWLWK 261

Query: 512 NTRPGKPN*P*DTV*THIRSRPRY 583
            T   +   P  TV   ++S  RY
Sbjct: 262 QTEGKEKFPPSFTVPLVLKSSLRY 285



 Score = 32.7 bits (71), Expect = 0.27
 Identities = 27/80 (33%), Positives = 31/80 (38%), Gaps = 10/80 (12%)
 Frame = +1

Query: 538 TLRYGMNPHQKPAQVFTTRDSLPITTLNGAP----------GFINLCDALNAWQLVKELK 687
           +LRYG NPHQK A  F    SL      G             + N  DA  AW  V E +
Sbjct: 282 SLRYGENPHQKAA--FYVDKSLAEVNAGGIATAIQHHGKEMSYNNYLDADAAWNCVSEFE 339

Query: 688 EALSLPAAASFKHVSPAGAA 747
                P     KH +P G A
Sbjct: 340 N----PTCVVVKHTNPCGVA 355



 Score = 28.3 bits (60), Expect = 5.8
 Identities = 13/31 (41%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
 Frame = +3

Query: 240 PVRPDVSKPD-VTVADAVENIDIGGVTLLRA 329
           P    V+ P  ++  D +ENIDIGG  ++RA
Sbjct: 173 PFYEKVTAPGGISFEDGIENIDIGGPAMIRA 203


>At1g64410.1 68414.m07301 hypothetical protein
          Length = 1231

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 14/28 (50%), Positives = 16/28 (57%)
 Frame = +2

Query: 335 KNHDRVTVVCDPADYDAVVKEIKENKHH 418
           K HDRVTV  +P D D   KE  E K +
Sbjct: 582 KGHDRVTVTVEPNDQDTAKKEKDEVKDY 609


>At1g52960.1 68414.m05990 hypothetical protein very low similarity
           to SP|Q9UUA2 DNA repair and recombination protein pif1,
           mitochondrial precursor {Schizosaccharomyces pombe}
          Length = 996

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 14/28 (50%), Positives = 16/28 (57%)
 Frame = +2

Query: 335 KNHDRVTVVCDPADYDAVVKEIKENKHH 418
           K HDRVTV  +P D D   KE  E K +
Sbjct: 208 KGHDRVTVTVEPNDQDTAKKEKDEVKDY 235


>At4g36980.1 68417.m05240 expressed protein
          Length = 560

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 16/48 (33%), Positives = 19/48 (39%)
 Frame = -2

Query: 235 GYRPPRLSSRTSDVSCLPGQSRIIELRSQHVPLDVKFSPDLRASPVLS 92
           GY PPR     SD+   P   RI+E        D+    D  A P  S
Sbjct: 355 GYSPPRSPPSQSDLLSRPSPGRILEALHVDPASDISLEKDKVAKPAKS 402


>At2g33060.1 68415.m04054 leucine-rich repeat family protein
           contains leucine rich-repeat domains Pfam:PF00560,
           INTERPRO:IPR001611; contains similarity to Hcr2-0B
           [Lycopersicon esculentum] gi|3894387|gb|AAC78593
          Length = 808

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 17/52 (32%), Positives = 28/52 (53%)
 Frame = -1

Query: 704 GRLSASFSSLTSCQAFSASHRLINPGAPFSVVIGKLSLVVNTWAGF*CGFIP 549
           G++ +SFS+L+       SH  +    PF   + KLS++V ++  F  G IP
Sbjct: 136 GQVPSSFSNLSQLNILDLSHNELTGSFPFVQNLTKLSILVLSYNHF-SGTIP 186


>At5g61100.1 68418.m07666 hypothetical protein
          Length = 227

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 14/45 (31%), Positives = 25/45 (55%)
 Frame = +2

Query: 77  MCRTSREHRRCSEVG*KLYIQRYMLGS*LDYPTLTRKT*NVRSTR 211
           MCR +REH  C+ V  +   + ++     D+ ++  KT N +S+R
Sbjct: 51  MCRDTREHTYCARVMFQRVPRLWICEECRDFSSVANKTANAQSSR 95


>At5g17980.1 68418.m02109 C2 domain-containing protein contains
           INTERPRO:IPR000008 C2 domain
          Length = 1049

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 16/55 (29%), Positives = 33/55 (60%), Gaps = 1/55 (1%)
 Frame = +3

Query: 252 DVSKPDVTVADAVENIDIGGVTLLRAQPRTTTGSP-SSVTRPTTML*SKKSKRTN 413
           DVS  + +  D VE +    + +++A+   T+GSP + ++   TM+ SK +++T+
Sbjct: 307 DVSTIERSTFDLVEKMHYVFIRVVKARSLPTSGSPVTKISLSGTMIQSKPARKTS 361


>At1g11780.1 68414.m01352 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein low similarity to alkB proteins from Homo
           sapiens [SP|Q13686], Caulobacter crescentus
           [GI:2055386]; contains Pfam profile PF03171:
           oxidoreductase, 2OG-Fe(II) oxygenase family
          Length = 345

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 13/35 (37%), Positives = 22/35 (62%)
 Frame = +1

Query: 595 DSLPITTLNGAPGFINLCDALNAWQLVKELKEALS 699
           DS P+  ++  PGF  + DAL+  +  K +KE+L+
Sbjct: 74  DSSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLT 108


>At1g04840.1 68414.m00480 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 665

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = +2

Query: 452 LKAFTHTSDYDLAISDYFRSNTRPGKPN 535
           +  F+ T DY+ AIS YF    +  KPN
Sbjct: 265 INGFSQTGDYETAISTYFEMLEKGLKPN 292


>At3g19670.1 68416.m02492 FF domain-containing protein / WW
           domain-containing protein weak similarity to
           huntingtin-interacting protein HYPA/FBP11 [Homo sapiens]
           GI:3341980; contains Pfam profiles PF01846: FF domain,
           PF00397: WW domain
          Length = 960

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 20/99 (20%), Positives = 41/99 (41%), Gaps = 5/99 (5%)
 Frame = +1

Query: 436 KAEISPEGVHSYFG-L*PRHIGLLPQQYSPGQAQLTLRYGMNPHQK----PAQVFTTRDS 600
           K   SP+G   Y+  +  +    +P++    + Q  +     PH +     ++V T  D+
Sbjct: 251 KEHSSPDGRKYYYNKITKQSTWTMPEEMKIVREQAEIASVQGPHAEGIIDASEVLTRSDT 310

Query: 601 LPITTLNGAPGFINLCDALNAWQLVKELKEALSLPAAAS 717
                  G P   +  + +    L  +LK+  S+P ++S
Sbjct: 311 ASTAAPTGLPSQTSTSEGVEKLTLTSDLKQPASVPGSSS 349


>At1g22240.1 68414.m02780 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile: PF00806 pumilio-family
           RNA binding domain
          Length = 515

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
 Frame = +1

Query: 520 PGQA-QLTLR-YGMNPHQKPAQVFTTRDSLPITTLNGAPGFINLCDALNAWQLVKELKEA 693
           PGQ  +++L  YG    Q+  +   TR  + +      PGF+NL   LN   +++   + 
Sbjct: 275 PGQLIRISLNAYGTRVVQRLVESIKTRKQISLVKSALRPGFLNLIRDLNGNHVIQRCLQC 334

Query: 694 LS 699
           LS
Sbjct: 335 LS 336


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,697,867
Number of Sequences: 28952
Number of extensions: 322240
Number of successful extensions: 1069
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1044
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1068
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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