BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021282 (770 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 87 3e-18 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 69 7e-13 SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc... 41 2e-04 SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 32 0.10 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 31 0.14 SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 28 1.3 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 27 2.2 SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc... 27 3.9 SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 27 3.9 SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces... 26 5.2 SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 5.2 SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm... 26 5.2 SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 26 6.9 SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosacch... 25 9.1 >SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 87.0 bits (206), Expect = 3e-18 Identities = 37/94 (39%), Positives = 65/94 (69%) Frame = +2 Query: 266 SFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLD 445 ++ +I +++L A++K N +A + FL K+ DV YF ++ EE+I++NFVL+YELLD Sbjct: 56 NYIYIHHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLD 115 Query: 446 EILDFGYPQNSDTGVLKTFITSRASSLPPRRNKP 547 EI+DFG+PQ ++T +L+ +IT ++++ P Sbjct: 116 EIMDFGFPQTTETKILQEYITQTSNTVKKHAPPP 149 Score = 46.8 bits (106), Expect = 3e-06 Identities = 19/42 (45%), Positives = 28/42 (66%) Frame = +3 Query: 645 LVDVTASQVLSAHVAGKVVMKSYLSGMPECKFGINDKIVMEA 770 L+ V+ + + GKV +K YLSGMPE + G+NDK++ EA Sbjct: 180 LIAAADGTVIQSEILGKVRLKCYLSGMPELRLGLNDKVLFEA 221 Score = 46.4 bits (105), Expect = 5e-06 Identities = 19/30 (63%), Positives = 24/30 (80%) Frame = +1 Query: 562 VTGQIGWRREGIKYRRNELFLDVLEYVNLL 651 +T I WR EGI YR+NE+FLDV+E VNL+ Sbjct: 152 MTNAISWRSEGIHYRKNEVFLDVIESVNLI 181 Score = 34.3 bits (75), Expect = 0.020 Identities = 16/32 (50%), Positives = 21/32 (65%) Frame = +3 Query: 108 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAF 203 M +FV N KG+V+ISR YR DI + V+ F Sbjct: 1 MASAIFVLNLKGKVIISRDYRADIPMSVVEKF 32 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 68.9 bits (161), Expect = 7e-13 Identities = 29/88 (32%), Positives = 54/88 (61%) Frame = +2 Query: 254 LARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIY 433 + +++ + K ++++ A+TK N N +V EFL +I + YFGK++E +K+N I+ Sbjct: 49 IGSSTYIYTKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIF 108 Query: 434 ELLDEILDFGYPQNSDTGVLKTFITSRA 517 ELLDE++D+G Q ++ L ++ A Sbjct: 109 ELLDEMIDYGIIQTTEPDALARSVSITA 136 Score = 49.6 bits (113), Expect = 5e-07 Identities = 19/46 (41%), Positives = 33/46 (71%) Frame = +1 Query: 520 KSASKEEQAQITSQVTGQIGWRREGIKYRRNELFLDVLEYVNLLMS 657 +S S + +S++ G + WRR GIKYR+N +++D++E +NLL+S Sbjct: 148 RSHSSQLAHTTSSEIPGSVPWRRAGIKYRKNSIYIDIVERMNLLIS 193 Score = 44.4 bits (100), Expect = 2e-05 Identities = 18/34 (52%), Positives = 26/34 (76%) Frame = +3 Query: 657 TASQVLSAHVAGKVVMKSYLSGMPECKFGINDKI 758 + VL + V+G V M++ LSGMPEC+FG+NDK+ Sbjct: 194 STGNVLRSDVSGVVKMRAMLSGMPECQFGLNDKL 227 Score = 42.3 bits (95), Expect = 7e-05 Identities = 17/50 (34%), Positives = 31/50 (62%) Frame = +3 Query: 108 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNL 257 MI GLF++N KG+ LI + +R D+ ++ + FRV ++ R P+ ++ Sbjct: 1 MISGLFIFNLKGDTLICKTFRHDLKKSVTEIFRVAIL-TNTDYRHPIVSI 49 >SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 425 Score = 41.1 bits (92), Expect = 2e-04 Identities = 16/72 (22%), Positives = 39/72 (54%) Frame = +2 Query: 290 NIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYP 469 ++ L T + + + + +I+DV++++FG + ++ N +I +LL E++D+GY Sbjct: 66 DVRLCIPTTCDTEPLYIHDIMRRIVDVVKTFFGGFNASKVEKNVCVIVQLLAEMIDYGYA 125 Query: 470 QNSDTGVLKTFI 505 + L+ + Sbjct: 126 TCMEPNALQDIV 137 Score = 30.3 bits (65), Expect = 0.32 Identities = 14/37 (37%), Positives = 19/37 (51%) Frame = +1 Query: 574 IGWRREGIKYRRNELFLDVLEYVNLLMSPQVKCSLLT 684 + WR KY NE F+ VLE V+ + P K + T Sbjct: 164 VPWRTAKAKYATNEFFIHVLERVSAVYQPNGKLAFGT 200 >SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 31.9 bits (69), Expect = 0.10 Identities = 13/56 (23%), Positives = 28/56 (50%) Frame = +2 Query: 287 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEIL 454 A ++ N + E + ++++ S+FG + E ++ NF + +LDEI+ Sbjct: 64 AGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEII 119 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 31.5 bits (68), Expect = 0.14 Identities = 17/55 (30%), Positives = 28/55 (50%) Frame = -1 Query: 476 NSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIPWPRSHFAL 312 +S + RS AT +S++S S + S T S+ ++ TQ+ WP +H L Sbjct: 719 DSGSRRVTRSQNATSQSQESGS--EIGSSIAGTAGSYNVGTSNTQLSWPSTHSTL 771 >SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual Length = 483 Score = 28.3 bits (60), Expect = 1.3 Identities = 12/46 (26%), Positives = 29/46 (63%) Frame = +2 Query: 275 HIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIK 412 +I+ N + + +++ + A++ E +++DV QS+ G + EEN++ Sbjct: 414 NIEITNPIIVSPVERSRSQALLNEIQTRVLDVRQSHGGVVLEENVR 459 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 27.5 bits (58), Expect = 2.2 Identities = 14/50 (28%), Positives = 20/50 (40%) Frame = -1 Query: 209 HSECVHSITTNIISVDSRDQHLAFMVINEQAPDHCG*GLTLFQNWFQCNC 60 H +C + T IS S + IN + P H + NW C+C Sbjct: 442 HKKCYPKVVTKCISKSSDSASSEYEKINHRIPHHFESHTNIGANWC-CHC 490 >SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 240 Score = 26.6 bits (56), Expect = 3.9 Identities = 15/71 (21%), Positives = 27/71 (38%) Frame = +2 Query: 293 IWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQ 472 +++ +T N + L + V+ S + E I I+ DE GY Sbjct: 64 LYIVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLGYRD 123 Query: 473 NSDTGVLKTFI 505 N +KT++ Sbjct: 124 NVSLTQIKTYL 134 >SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces pombe|chr 3|||Manual Length = 1364 Score = 26.6 bits (56), Expect = 3.9 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +2 Query: 374 QSYFGKISEENIKNNFVLIYELLDEILDFGYPQNSDTGVLKTFITSRA 517 + + KI EE +K N +L YE D +S ++KTF +A Sbjct: 952 KDFVTKIEEETLKENPLLFYEYRKASSDMRVLMDSQFLMMKTFARLQA 999 >SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 180 Score = 26.2 bits (55), Expect = 5.2 Identities = 11/37 (29%), Positives = 23/37 (62%) Frame = +2 Query: 368 VMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 478 V++++ G+I+++ ++ V+IY + D L FG S Sbjct: 113 VLKAHVGRITDDTPQHQGVVIYSMNDTPLGFGVTARS 149 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 26.2 bits (55), Expect = 5.2 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = +3 Query: 213 VIHARQQVRSPVTNLHAHLSSISSVQIFGWQQSQSK 320 V+ Q + SP T++ +LS ++F W + Q K Sbjct: 3556 VMSIAQSLSSPTTDIKQYLSLYVRDEVFWWSKQQRK 3591 >SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1292 Score = 26.2 bits (55), Expect = 5.2 Identities = 17/54 (31%), Positives = 24/54 (44%) Frame = -1 Query: 497 SSGPQYQNSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIP 336 SS P +SE NQ P S Q +Y+ ++ S + SS+GT P Sbjct: 10 SSRPPSVHSERNQKPSSSQFLGVPSSNYNQRENSSRSGSSTISREPSSSGTMYP 63 >SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 25.8 bits (54), Expect = 6.9 Identities = 16/58 (27%), Positives = 28/58 (48%) Frame = +3 Query: 24 YVQFLKGCNR*IAITLKPILKECEASPTMIGGLFVYNHKGEVLISRVYRDDIGRNAVD 197 +V ++ G R I + L P+L PT+ +NH G++ + R + G N+ D Sbjct: 737 HVSYVTGVIRSIDLQL-PVLLSKFMKPTIFDSYDFFNHWGQMGVEREAQLTFGLNSKD 793 >SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosaccharomyces pombe|chr 1|||Manual Length = 1030 Score = 25.4 bits (53), Expect = 9.1 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = -2 Query: 352 QELKYHGRVHILLCDCCQPNICTLDMEERCACKLVTGD 239 +ELK RV L C N+ +D++ R AC++ +GD Sbjct: 836 RELKIRQRVLRRLGFCTLENV--IDIKGRVACEITSGD 871 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,308,328 Number of Sequences: 5004 Number of extensions: 70194 Number of successful extensions: 205 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 193 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 205 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 371330890 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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