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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021282
         (770 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc...    87   3e-18
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac...    69   7e-13
SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc...    41   2e-04
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc...    32   0.10 
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces...    31   0.14 
SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual      28   1.3  
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom...    27   2.2  
SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc...    27   3.9  
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ...    27   3.9  
SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces...    26   5.2  
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ...    26   5.2  
SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm...    26   5.2  
SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc...    26   6.9  
SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosacch...    25   9.1  

>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 426

 Score = 87.0 bits (206), Expect = 3e-18
 Identities = 37/94 (39%), Positives = 65/94 (69%)
 Frame = +2

Query: 266 SFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLD 445
           ++ +I   +++L A++K N +A  +  FL K+ DV   YF ++ EE+I++NFVL+YELLD
Sbjct: 56  NYIYIHHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLD 115

Query: 446 EILDFGYPQNSDTGVLKTFITSRASSLPPRRNKP 547
           EI+DFG+PQ ++T +L+ +IT  ++++      P
Sbjct: 116 EIMDFGFPQTTETKILQEYITQTSNTVKKHAPPP 149



 Score = 46.8 bits (106), Expect = 3e-06
 Identities = 19/42 (45%), Positives = 28/42 (66%)
 Frame = +3

Query: 645 LVDVTASQVLSAHVAGKVVMKSYLSGMPECKFGINDKIVMEA 770
           L+      V+ + + GKV +K YLSGMPE + G+NDK++ EA
Sbjct: 180 LIAAADGTVIQSEILGKVRLKCYLSGMPELRLGLNDKVLFEA 221



 Score = 46.4 bits (105), Expect = 5e-06
 Identities = 19/30 (63%), Positives = 24/30 (80%)
 Frame = +1

Query: 562 VTGQIGWRREGIKYRRNELFLDVLEYVNLL 651
           +T  I WR EGI YR+NE+FLDV+E VNL+
Sbjct: 152 MTNAISWRSEGIHYRKNEVFLDVIESVNLI 181



 Score = 34.3 bits (75), Expect = 0.020
 Identities = 16/32 (50%), Positives = 21/32 (65%)
 Frame = +3

Query: 108 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAF 203
           M   +FV N KG+V+ISR YR DI  + V+ F
Sbjct: 1   MASAIFVLNLKGKVIISRDYRADIPMSVVEKF 32


>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 446

 Score = 68.9 bits (161), Expect = 7e-13
 Identities = 29/88 (32%), Positives = 54/88 (61%)
 Frame = +2

Query: 254 LARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIY 433
           +  +++ + K  ++++ A+TK N N  +V EFL  +I  +  YFGK++E  +K+N   I+
Sbjct: 49  IGSSTYIYTKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIF 108

Query: 434 ELLDEILDFGYPQNSDTGVLKTFITSRA 517
           ELLDE++D+G  Q ++   L   ++  A
Sbjct: 109 ELLDEMIDYGIIQTTEPDALARSVSITA 136



 Score = 49.6 bits (113), Expect = 5e-07
 Identities = 19/46 (41%), Positives = 33/46 (71%)
 Frame = +1

Query: 520 KSASKEEQAQITSQVTGQIGWRREGIKYRRNELFLDVLEYVNLLMS 657
           +S S +     +S++ G + WRR GIKYR+N +++D++E +NLL+S
Sbjct: 148 RSHSSQLAHTTSSEIPGSVPWRRAGIKYRKNSIYIDIVERMNLLIS 193



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 18/34 (52%), Positives = 26/34 (76%)
 Frame = +3

Query: 657 TASQVLSAHVAGKVVMKSYLSGMPECKFGINDKI 758
           +   VL + V+G V M++ LSGMPEC+FG+NDK+
Sbjct: 194 STGNVLRSDVSGVVKMRAMLSGMPECQFGLNDKL 227



 Score = 42.3 bits (95), Expect = 7e-05
 Identities = 17/50 (34%), Positives = 31/50 (62%)
 Frame = +3

Query: 108 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNL 257
           MI GLF++N KG+ LI + +R D+ ++  + FRV ++      R P+ ++
Sbjct: 1   MISGLFIFNLKGDTLICKTFRHDLKKSVTEIFRVAIL-TNTDYRHPIVSI 49


>SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 425

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 16/72 (22%), Positives = 39/72 (54%)
 Frame = +2

Query: 290 NIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYP 469
           ++ L   T  +     + + + +I+DV++++FG  +   ++ N  +I +LL E++D+GY 
Sbjct: 66  DVRLCIPTTCDTEPLYIHDIMRRIVDVVKTFFGGFNASKVEKNVCVIVQLLAEMIDYGYA 125

Query: 470 QNSDTGVLKTFI 505
              +   L+  +
Sbjct: 126 TCMEPNALQDIV 137



 Score = 30.3 bits (65), Expect = 0.32
 Identities = 14/37 (37%), Positives = 19/37 (51%)
 Frame = +1

Query: 574 IGWRREGIKYRRNELFLDVLEYVNLLMSPQVKCSLLT 684
           + WR    KY  NE F+ VLE V+ +  P  K +  T
Sbjct: 164 VPWRTAKAKYATNEFFIHVLERVSAVYQPNGKLAFGT 200


>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 143

 Score = 31.9 bits (69), Expect = 0.10
 Identities = 13/56 (23%), Positives = 28/56 (50%)
 Frame = +2

Query: 287 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEIL 454
           A ++         N   + E +   ++++ S+FG + E ++  NF  +  +LDEI+
Sbjct: 64  AGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEII 119


>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 879

 Score = 31.5 bits (68), Expect = 0.14
 Identities = 17/55 (30%), Positives = 28/55 (50%)
 Frame = -1

Query: 476 NSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIPWPRSHFAL 312
           +S   +  RS  AT +S++S S   +  S   T  S+   ++ TQ+ WP +H  L
Sbjct: 719 DSGSRRVTRSQNATSQSQESGS--EIGSSIAGTAGSYNVGTSNTQLSWPSTHSTL 771


>SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 483

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 12/46 (26%), Positives = 29/46 (63%)
 Frame = +2

Query: 275 HIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIK 412
           +I+  N  + +  +++ + A++ E   +++DV QS+ G + EEN++
Sbjct: 414 NIEITNPIIVSPVERSRSQALLNEIQTRVLDVRQSHGGVVLEENVR 459


>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1016

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 14/50 (28%), Positives = 20/50 (40%)
 Frame = -1

Query: 209 HSECVHSITTNIISVDSRDQHLAFMVINEQAPDHCG*GLTLFQNWFQCNC 60
           H +C   + T  IS  S      +  IN + P H      +  NW  C+C
Sbjct: 442 HKKCYPKVVTKCISKSSDSASSEYEKINHRIPHHFESHTNIGANWC-CHC 490


>SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 240

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 15/71 (21%), Positives = 27/71 (38%)
 Frame = +2

Query: 293 IWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQ 472
           +++  +T    N     + L  +  V+ S    + E  I      I+   DE    GY  
Sbjct: 64  LYIVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLGYRD 123

Query: 473 NSDTGVLKTFI 505
           N     +KT++
Sbjct: 124 NVSLTQIKTYL 134


>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1364

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 15/48 (31%), Positives = 23/48 (47%)
 Frame = +2

Query: 374  QSYFGKISEENIKNNFVLIYELLDEILDFGYPQNSDTGVLKTFITSRA 517
            + +  KI EE +K N +L YE      D     +S   ++KTF   +A
Sbjct: 952  KDFVTKIEEETLKENPLLFYEYRKASSDMRVLMDSQFLMMKTFARLQA 999


>SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 180

 Score = 26.2 bits (55), Expect = 5.2
 Identities = 11/37 (29%), Positives = 23/37 (62%)
 Frame = +2

Query: 368 VMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 478
           V++++ G+I+++  ++  V+IY + D  L FG    S
Sbjct: 113 VLKAHVGRITDDTPQHQGVVIYSMNDTPLGFGVTARS 149


>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 3655

 Score = 26.2 bits (55), Expect = 5.2
 Identities = 11/36 (30%), Positives = 19/36 (52%)
 Frame = +3

Query: 213  VIHARQQVRSPVTNLHAHLSSISSVQIFGWQQSQSK 320
            V+   Q + SP T++  +LS     ++F W + Q K
Sbjct: 3556 VMSIAQSLSSPTTDIKQYLSLYVRDEVFWWSKQQRK 3591


>SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1292

 Score = 26.2 bits (55), Expect = 5.2
 Identities = 17/54 (31%), Positives = 24/54 (44%)
 Frame = -1

Query: 497 SSGPQYQNSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIP 336
           SS P   +SE NQ P S Q       +Y+         ++  S + SS+GT  P
Sbjct: 10  SSRPPSVHSERNQKPSSSQFLGVPSSNYNQRENSSRSGSSTISREPSSSGTMYP 63


>SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 878

 Score = 25.8 bits (54), Expect = 6.9
 Identities = 16/58 (27%), Positives = 28/58 (48%)
 Frame = +3

Query: 24  YVQFLKGCNR*IAITLKPILKECEASPTMIGGLFVYNHKGEVLISRVYRDDIGRNAVD 197
           +V ++ G  R I + L P+L      PT+      +NH G++ + R  +   G N+ D
Sbjct: 737 HVSYVTGVIRSIDLQL-PVLLSKFMKPTIFDSYDFFNHWGQMGVEREAQLTFGLNSKD 793


>SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1030

 Score = 25.4 bits (53), Expect = 9.1
 Identities = 14/38 (36%), Positives = 22/38 (57%)
 Frame = -2

Query: 352 QELKYHGRVHILLCDCCQPNICTLDMEERCACKLVTGD 239
           +ELK   RV   L  C   N+  +D++ R AC++ +GD
Sbjct: 836 RELKIRQRVLRRLGFCTLENV--IDIKGRVACEITSGD 871


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,308,328
Number of Sequences: 5004
Number of extensions: 70194
Number of successful extensions: 205
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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