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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021280
         (701 letters)

Database: fruitfly 
           53,049 sequences; 24,988,368 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X17298-1|CAA35185.1| 1129|Drosophila melanogaster RNA polymerase...    86   4e-17
AY075569-1|AAL68376.1| 1129|Drosophila melanogaster SD02110p pro...    86   4e-17
AE014134-97|AAF51503.1| 1129|Drosophila melanogaster CG4033-PA p...    86   4e-17
AY070546-1|AAL48017.1|  564|Drosophila melanogaster LD27030p pro...    29   6.1  
AE013599-592|AAF59131.1|  564|Drosophila melanogaster CG2158-PA ...    29   6.1  
M59501-1|AAA28994.1|  462|Drosophila melanogaster tube protein p...    29   8.1  

>X17298-1|CAA35185.1| 1129|Drosophila melanogaster RNA polymerase 3,
           second  largestsubunit protein.
          Length = 1129

 Score = 86.2 bits (204), Expect = 4e-17
 Identities = 64/196 (32%), Positives = 101/196 (51%), Gaps = 9/196 (4%)
 Frame = +2

Query: 140 SHPDYRKPPKIANPYLQSLGAPHIDSFNYMLDDGLKFAKLIYCHPNLSYLVAKRSK*QSM 319
           S P++++ PK  + +L +LG PH+DSF+ ML  GL  +   +  PN  + ++   +  SM
Sbjct: 17  SRPEFKQIPKKLSRHLANLGGPHVDSFDEMLTVGLDNSAK-HMIPN--HWLSPAGEKISM 73

Query: 320 KQHSLNPMFPWI-Q*GLNLRWSSQQNAGRELLHTRES*RSESLCALTEGLS-------PL 475
           K  S+    P + Q  +++R           LH   S     +C++  G S       P+
Sbjct: 74  KVESIWIAKPKVPQDVIDVRTREIYPTDSRQLHVSYS----GMCSVRLGWSVNGVQKTPI 129

Query: 476 RDP*VIYPL*LVQNV-SFG*FIT*GIVEKNEHADEWGGYFVIKGHERLARMLLVTRRNYP 652
                  P+ L     + G      +V+  EH  EWGG FVI+G+E++ RML++TRRN+P
Sbjct: 130 NMDLGEVPIMLRSKACNLGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHP 189

Query: 653 VAIKRSGWKMRGNLFS 700
           + +KRS WK RG  FS
Sbjct: 190 ICVKRSSWKDRGQNFS 205



 Score = 62.5 bits (145), Expect = 5e-10
 Identities = 27/86 (31%), Positives = 50/86 (58%), Gaps = 1/86 (1%)
 Frame = +1

Query: 262 LLPSEFELPSGEKVKVTIDEAAFAKPNVPMDTVGVKSQVVLPTECRQRAATYKGELKIRV 441
           ++P+ +  P+GEK+ + ++    AKP VP D + V+++ + PT+ RQ   +Y G   +R+
Sbjct: 58  MIPNHWLSPAGEKISMKVESIWIAKPKVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRL 117

Query: 442 TLCIDG-RSVTIERSLGYLPIMISPK 516
              ++G +   I   LG +PIM+  K
Sbjct: 118 GWSVNGVQKTPINMDLGEVPIMLRSK 143


>AY075569-1|AAL68376.1| 1129|Drosophila melanogaster SD02110p
           protein.
          Length = 1129

 Score = 86.2 bits (204), Expect = 4e-17
 Identities = 64/196 (32%), Positives = 101/196 (51%), Gaps = 9/196 (4%)
 Frame = +2

Query: 140 SHPDYRKPPKIANPYLQSLGAPHIDSFNYMLDDGLKFAKLIYCHPNLSYLVAKRSK*QSM 319
           S P++++ PK  + +L +LG PH+DSF+ ML  GL  +   +  PN  + ++   +  SM
Sbjct: 17  SRPEFKQIPKKLSRHLANLGGPHVDSFDEMLTVGLDNSAK-HMIPN--HWLSPAGEKISM 73

Query: 320 KQHSLNPMFPWI-Q*GLNLRWSSQQNAGRELLHTRES*RSESLCALTEGLS-------PL 475
           K  S+    P + Q  +++R           LH   S     +C++  G S       P+
Sbjct: 74  KVESIWIAKPKVPQDVIDVRTREIYPTDSRQLHVSYS----GMCSVRLGWSVNGVQKTPI 129

Query: 476 RDP*VIYPL*LVQNV-SFG*FIT*GIVEKNEHADEWGGYFVIKGHERLARMLLVTRRNYP 652
                  P+ L     + G      +V+  EH  EWGG FVI+G+E++ RML++TRRN+P
Sbjct: 130 NMDLGEVPIMLRSKACNLGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHP 189

Query: 653 VAIKRSGWKMRGNLFS 700
           + +KRS WK RG  FS
Sbjct: 190 ICVKRSSWKDRGQNFS 205



 Score = 62.5 bits (145), Expect = 5e-10
 Identities = 27/86 (31%), Positives = 50/86 (58%), Gaps = 1/86 (1%)
 Frame = +1

Query: 262 LLPSEFELPSGEKVKVTIDEAAFAKPNVPMDTVGVKSQVVLPTECRQRAATYKGELKIRV 441
           ++P+ +  P+GEK+ + ++    AKP VP D + V+++ + PT+ RQ   +Y G   +R+
Sbjct: 58  MIPNHWLSPAGEKISMKVESIWIAKPKVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRL 117

Query: 442 TLCIDG-RSVTIERSLGYLPIMISPK 516
              ++G +   I   LG +PIM+  K
Sbjct: 118 GWSVNGVQKTPINMDLGEVPIMLRSK 143


>AE014134-97|AAF51503.1| 1129|Drosophila melanogaster CG4033-PA
           protein.
          Length = 1129

 Score = 86.2 bits (204), Expect = 4e-17
 Identities = 64/196 (32%), Positives = 101/196 (51%), Gaps = 9/196 (4%)
 Frame = +2

Query: 140 SHPDYRKPPKIANPYLQSLGAPHIDSFNYMLDDGLKFAKLIYCHPNLSYLVAKRSK*QSM 319
           S P++++ PK  + +L +LG PH+DSF+ ML  GL  +   +  PN  + ++   +  SM
Sbjct: 17  SRPEFKQIPKKLSRHLANLGGPHVDSFDEMLTVGLDNSAK-HMIPN--HWLSPAGEKISM 73

Query: 320 KQHSLNPMFPWI-Q*GLNLRWSSQQNAGRELLHTRES*RSESLCALTEGLS-------PL 475
           K  S+    P + Q  +++R           LH   S     +C++  G S       P+
Sbjct: 74  KVESIWIAKPKVPQDVIDVRTREIYPTDSRQLHVSYS----GMCSVRLGWSVNGVQKTPI 129

Query: 476 RDP*VIYPL*LVQNV-SFG*FIT*GIVEKNEHADEWGGYFVIKGHERLARMLLVTRRNYP 652
                  P+ L     + G      +V+  EH  EWGG FVI+G+E++ RML++TRRN+P
Sbjct: 130 NMDLGEVPIMLRSKACNLGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHP 189

Query: 653 VAIKRSGWKMRGNLFS 700
           + +KRS WK RG  FS
Sbjct: 190 ICVKRSSWKDRGQNFS 205



 Score = 62.5 bits (145), Expect = 5e-10
 Identities = 27/86 (31%), Positives = 50/86 (58%), Gaps = 1/86 (1%)
 Frame = +1

Query: 262 LLPSEFELPSGEKVKVTIDEAAFAKPNVPMDTVGVKSQVVLPTECRQRAATYKGELKIRV 441
           ++P+ +  P+GEK+ + ++    AKP VP D + V+++ + PT+ RQ   +Y G   +R+
Sbjct: 58  MIPNHWLSPAGEKISMKVESIWIAKPKVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRL 117

Query: 442 TLCIDG-RSVTIERSLGYLPIMISPK 516
              ++G +   I   LG +PIM+  K
Sbjct: 118 GWSVNGVQKTPINMDLGEVPIMLRSK 143


>AY070546-1|AAL48017.1|  564|Drosophila melanogaster LD27030p
           protein.
          Length = 564

 Score = 29.1 bits (62), Expect = 6.1
 Identities = 23/57 (40%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
 Frame = -1

Query: 425 SPLYVAALCR-HSVGRTT*DLTPTVSMGTLGLANA----ASSIVTLTFSPLGNSNSD 270
           SPL     C   S G TT   TP VS G+     A    +SSI +LT  P G + SD
Sbjct: 290 SPLAKKPNCTITSGGTTTTTATPLVSFGSTASFTAPVPSSSSIFSLTAKPTGEAKSD 346


>AE013599-592|AAF59131.1|  564|Drosophila melanogaster CG2158-PA
           protein.
          Length = 564

 Score = 29.1 bits (62), Expect = 6.1
 Identities = 23/57 (40%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
 Frame = -1

Query: 425 SPLYVAALCR-HSVGRTT*DLTPTVSMGTLGLANA----ASSIVTLTFSPLGNSNSD 270
           SPL     C   S G TT   TP VS G+     A    +SSI +LT  P G + SD
Sbjct: 290 SPLAKKPNCTITSGGTTTTTATPLVSFGSTASFTAPVPSSSSIFSLTAKPTGEAKSD 346


>M59501-1|AAA28994.1|  462|Drosophila melanogaster tube protein
           protein.
          Length = 462

 Score = 28.7 bits (61), Expect = 8.1
 Identities = 15/55 (27%), Positives = 29/55 (52%)
 Frame = -1

Query: 488 PKDLSMVTDLPSMHRVTLIFNSPLYVAALCRHSVGRTT*DLTPTVSMGTLGLANA 324
           P + +  TD P++ R+TL+ ++   V +   H+  + +   TPT S   L + +A
Sbjct: 308 PSNRTSSTDPPNIPRITLLIDNSGDVNSRPNHAPAKASTATTPTASSNNLPMISA 362


  Database: fruitfly
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 24,988,368
  Number of sequences in database:  53,049
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 32,598,415
Number of Sequences: 53049
Number of extensions: 697997
Number of successful extensions: 1719
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1648
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1719
length of database: 24,988,368
effective HSP length: 83
effective length of database: 20,585,301
effective search space used: 3087795150
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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