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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021280
         (701 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g29940.1 68414.m03658 DNA-directed RNA polymerase family prot...    40   0.001
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea...    31   0.74 
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl...    28   5.2  
At5g56010.1 68418.m06989 heat shock protein, putative strong sim...    28   5.2  
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl...    28   5.2  
At5g04170.1 68418.m00405 calcium-binding EF hand family protein ...    28   5.2  

>At1g29940.1 68414.m03658 DNA-directed RNA polymerase family protein
           similar to SP|P22138 DNA-directed RNA polymerase I 135
           kDa polypeptide (EC 2.7.7.6) (RNA polymerase I subunit
           2) {Saccharomyces cerevisiae}; contains Pfam profiles
           PF04563; RNA polymerase beta subunit, PF04560: RNA
           polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2
           domain 2, PF04565: RNA polymerase Rpb2 domain 3,
           PF00562: RNA polymerase Rpb2 domain 6
          Length = 1114

 Score = 40.3 bits (90), Expect = 0.001
 Identities = 16/51 (31%), Positives = 32/51 (62%)
 Frame = +2

Query: 548 IVEKNEHADEWGGYFVIKGHERLARMLLVTRRNYPVAIKRSGWKMRGNLFS 700
           +++  E   E GGYF++ G ER+ R ++  +RN+P ++ R+ ++ R   +S
Sbjct: 93  LLKCKESTSEMGGYFILNGIERVFRCVIAPKRNHPTSMIRNSFRDRKEGYS 143


>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
           shock protein 83 (HSP83) nearly identical to SP|P27323
           Heat shock protein 81-1 (HSP81-1) (Heat shock protein
           83) {Arabidopsis thaliana}; contains Pfam profiles
           PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
           HSP90-like domain protein, PF00183: Hsp90 protein
          Length = 705

 Score = 31.1 bits (67), Expect = 0.74
 Identities = 14/36 (38%), Positives = 18/36 (50%), Gaps = 2/36 (5%)
 Frame = -3

Query: 387 WEDHLRFNPYCIHGNIGFSECCFID--CHFDLFATR 286
           WEDHL    + + G + F    F+     FDLF TR
Sbjct: 297 WEDHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTR 332


>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
           identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
           {Arabidopsis thaliana}
          Length = 699

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 12/36 (33%), Positives = 18/36 (50%), Gaps = 2/36 (5%)
 Frame = -3

Query: 387 WEDHLRFNPYCIHGNIGFSECCFID--CHFDLFATR 286
           WE+HL    + + G + F    F+     FDLF T+
Sbjct: 291 WEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTK 326


>At5g56010.1 68418.m06989 heat shock protein, putative strong
           similarity to SP|P55737 Heat shock protein 81-2
           (HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
           PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
           HSP90-like domain protein, PF00183: Hsp90 protein
          Length = 699

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 12/36 (33%), Positives = 18/36 (50%), Gaps = 2/36 (5%)
 Frame = -3

Query: 387 WEDHLRFNPYCIHGNIGFSECCFID--CHFDLFATR 286
           WE+HL    + + G + F    F+     FDLF T+
Sbjct: 291 WEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTK 326


>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
           identical to heat shock protein hsp81.4 [Arabidopsis
           thaliana] GI:1906828; contains Pfam profiles PF02518:
           ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
           domain protein, PF00183: Hsp90 protein
          Length = 699

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 12/36 (33%), Positives = 18/36 (50%), Gaps = 2/36 (5%)
 Frame = -3

Query: 387 WEDHLRFNPYCIHGNIGFSECCFID--CHFDLFATR 286
           WE+HL    + + G + F    F+     FDLF T+
Sbjct: 291 WEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTK 326


>At5g04170.1 68418.m00405 calcium-binding EF hand family protein low
           similarity to peflin [Homo sapiens] GI:6015440; contains
           INTERPRO:IPR002048 calcium-binding EF-hand domain
          Length = 354

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 16/32 (50%), Positives = 16/32 (50%), Gaps = 1/32 (3%)
 Frame = +2

Query: 113 AQNPSLAYTSHP-DYRKPPKIANPYLQSLGAP 205
           A  PS  Y   P DY KPPK   PY    GAP
Sbjct: 72  APPPSAPYAPSPGDYNKPPK-EKPYGGGYGAP 102


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,829,198
Number of Sequences: 28952
Number of extensions: 335711
Number of successful extensions: 780
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 780
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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