BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021280 (701 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g29940.1 68414.m03658 DNA-directed RNA polymerase family prot... 40 0.001 At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 31 0.74 At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 28 5.2 At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 28 5.2 At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 28 5.2 At5g04170.1 68418.m00405 calcium-binding EF hand family protein ... 28 5.2 >At1g29940.1 68414.m03658 DNA-directed RNA polymerase family protein similar to SP|P22138 DNA-directed RNA polymerase I 135 kDa polypeptide (EC 2.7.7.6) (RNA polymerase I subunit 2) {Saccharomyces cerevisiae}; contains Pfam profiles PF04563; RNA polymerase beta subunit, PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2 domain 3, PF00562: RNA polymerase Rpb2 domain 6 Length = 1114 Score = 40.3 bits (90), Expect = 0.001 Identities = 16/51 (31%), Positives = 32/51 (62%) Frame = +2 Query: 548 IVEKNEHADEWGGYFVIKGHERLARMLLVTRRNYPVAIKRSGWKMRGNLFS 700 +++ E E GGYF++ G ER+ R ++ +RN+P ++ R+ ++ R +S Sbjct: 93 LLKCKESTSEMGGYFILNGIERVFRCVIAPKRNHPTSMIRNSFRDRKEGYS 143 >At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat shock protein 83 (HSP83) nearly identical to SP|P27323 Heat shock protein 81-1 (HSP81-1) (Heat shock protein 83) {Arabidopsis thaliana}; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 705 Score = 31.1 bits (67), Expect = 0.74 Identities = 14/36 (38%), Positives = 18/36 (50%), Gaps = 2/36 (5%) Frame = -3 Query: 387 WEDHLRFNPYCIHGNIGFSECCFID--CHFDLFATR 286 WEDHL + + G + F F+ FDLF TR Sbjct: 297 WEDHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTR 332 >At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly identical to SP|P55737 Heat shock protein 81-2 (HSP81-2) {Arabidopsis thaliana} Length = 699 Score = 28.3 bits (60), Expect = 5.2 Identities = 12/36 (33%), Positives = 18/36 (50%), Gaps = 2/36 (5%) Frame = -3 Query: 387 WEDHLRFNPYCIHGNIGFSECCFID--CHFDLFATR 286 WE+HL + + G + F F+ FDLF T+ Sbjct: 291 WEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTK 326 >At5g56010.1 68418.m06989 heat shock protein, putative strong similarity to SP|P55737 Heat shock protein 81-2 (HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 699 Score = 28.3 bits (60), Expect = 5.2 Identities = 12/36 (33%), Positives = 18/36 (50%), Gaps = 2/36 (5%) Frame = -3 Query: 387 WEDHLRFNPYCIHGNIGFSECCFID--CHFDLFATR 286 WE+HL + + G + F F+ FDLF T+ Sbjct: 291 WEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTK 326 >At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly identical to heat shock protein hsp81.4 [Arabidopsis thaliana] GI:1906828; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 699 Score = 28.3 bits (60), Expect = 5.2 Identities = 12/36 (33%), Positives = 18/36 (50%), Gaps = 2/36 (5%) Frame = -3 Query: 387 WEDHLRFNPYCIHGNIGFSECCFID--CHFDLFATR 286 WE+HL + + G + F F+ FDLF T+ Sbjct: 291 WEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTK 326 >At5g04170.1 68418.m00405 calcium-binding EF hand family protein low similarity to peflin [Homo sapiens] GI:6015440; contains INTERPRO:IPR002048 calcium-binding EF-hand domain Length = 354 Score = 28.3 bits (60), Expect = 5.2 Identities = 16/32 (50%), Positives = 16/32 (50%), Gaps = 1/32 (3%) Frame = +2 Query: 113 AQNPSLAYTSHP-DYRKPPKIANPYLQSLGAP 205 A PS Y P DY KPPK PY GAP Sbjct: 72 APPPSAPYAPSPGDYNKPPK-EKPYGGGYGAP 102 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,829,198 Number of Sequences: 28952 Number of extensions: 335711 Number of successful extensions: 780 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 759 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 780 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1506636208 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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