Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021274
(734 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 138 6e-34
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 133 2e-32
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 122 4e-29
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 121 1e-28
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 113 3e-26
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 60 4e-10
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 43 4e-05
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 29 0.69
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 29 0.91
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.91
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 26 4.8
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 8.5
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 138 bits (335), Expect = 6e-34
Identities = 63/88 (71%), Positives = 73/88 (82%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P IFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSM
Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
VL KM+ETAEAYLG V +AV+TVPAYF
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYF 148
Score = 136 bits (330), Expect = 2e-33
Identities = 65/76 (85%), Positives = 71/76 (93%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 686
+DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLIFDLGGGTFDVS+L
Sbjct: 149 NDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDVSLL 208
Query: 687 TIEDGIFEVKSTAGDT 734
TIE+GIFEVK+TAGDT
Sbjct: 209 TIEEGIFEVKATAGDT 224
Score = 113 bits (273), Expect = 2e-26
Identities = 51/60 (85%), Positives = 57/60 (95%)
Frame = +2
Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTDRLIGDAAKNQVAMNPNN 253
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDT+RLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 133 bits (322), Expect = 2e-32
Identities = 64/76 (84%), Positives = 70/76 (92%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 686
+DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLIFDLGGGTFDVS+L
Sbjct: 149 NDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDVSLL 208
Query: 687 TIEDGIFEVKSTAGDT 734
TIE+GIFEVK+TAGDT
Sbjct: 209 TIEEGIFEVKATAGDT 224
Score = 131 bits (316), Expect = 1e-31
Identities = 59/88 (67%), Positives = 71/88 (80%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P IFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSM
Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
VL KM+E+AEA+LG V +AV+TVPAYF
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYF 148
Score = 113 bits (273), Expect = 2e-26
Identities = 51/60 (85%), Positives = 57/60 (95%)
Frame = +2
Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTDRLIGDAAKNQVAMNPNN 253
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDT+RLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 122 bits (295), Expect = 4e-29
Identities = 59/75 (78%), Positives = 65/75 (86%)
Frame = +3
Query: 510 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 689
DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLIFDLGGGTFDVS+L
Sbjct: 154 DSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFDVSLLK 213
Query: 690 IEDGIFEVKSTAGDT 734
I+ G+FEV +TAGDT
Sbjct: 214 IQGGVFEVLATAGDT 228
Score = 118 bits (283), Expect = 1e-27
Identities = 53/88 (60%), Positives = 67/88 (76%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+M
Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
VLTKMKE +EA L K V+ AVITVPAYF
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYF 152
Score = 101 bits (242), Expect = 1e-22
Identities = 45/60 (75%), Positives = 52/60 (86%)
Frame = +2
Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTDRLIGDAAKNQVAMNPNN 253
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+T+RL+GDAAKNQ AMNP N
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 121 bits (291), Expect = 1e-28
Identities = 54/76 (71%), Positives = 72/76 (94%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 686
+D+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK T E++++++DLGGGTFDVS+L
Sbjct: 182 NDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYDLGGGTFDVSLL 240
Query: 687 TIEDGIFEVKSTAGDT 734
+I++G+FEV +T+GDT
Sbjct: 241 SIDNGVFEVLATSGDT 256
Score = 113 bits (273), Expect = 2e-26
Identities = 50/88 (56%), Positives = 69/88 (78%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ IFD KRLIGRKF++ T+ D+K +PF +V+D +P ++V G+ K F PEE+S+M
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
+L+KMK+TAEAYLGK V +AV+TVPAYF
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYF 181
Score = 99.5 bits (237), Expect = 4e-22
Identities = 43/59 (72%), Positives = 51/59 (86%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTDRLIGDAAKNQVAMNPNN 253
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ +RL+G+AAKNQ NP N
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPEN 96
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 113 bits (271), Expect = 3e-26
Identities = 53/76 (69%), Positives = 65/76 (85%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 686
+DSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGTFD+SIL
Sbjct: 195 NDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFDLGGGTFDISIL 252
Query: 687 TIEDGIFEVKSTAGDT 734
+ +G+FEV+ST GDT
Sbjct: 253 ELNNGVFEVRSTNGDT 268
Score = 82.6 bits (195), Expect = 5e-17
Identities = 38/88 (43%), Positives = 55/88 (62%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ F KRLIGR+F++ VQ D+K P+++V + KT+ P ++
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
+L+KM+ETA YLGK V+NAV+TVPAYF
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYF 194
Score = 74.9 bits (176), Expect = 1e-14
Identities = 34/66 (51%), Positives = 46/66 (69%), Gaps = 1/66 (1%)
Frame = +2
Query: 65 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTDRLIGDAAKNQVAM 241
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D +RL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 242 NPNNKY 259
NP N +
Sbjct: 109 NPENTF 114
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 59.7 bits (138), Expect = 4e-10
Identities = 33/78 (42%), Positives = 44/78 (56%), Gaps = 4/78 (5%)
Frame = +3
Query: 510 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLGGGTFDV 677
D QR+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G + V
Sbjct: 154 DIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNYSV 213
Query: 678 SILTIEDGIFEVKSTAGD 731
SI+ G F +KST D
Sbjct: 214 SIVEFSRGQFHIKSTVCD 231
Score = 52.0 bits (119), Expect = 9e-08
Identities = 22/64 (34%), Positives = 40/64 (62%)
Frame = +2
Query: 62 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTDRLIGDAAKNQVAM 241
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62
Query: 242 NPNN 253
N N
Sbjct: 63 NFRN 66
Score = 41.5 bits (93), Expect = 1e-04
Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 1/85 (1%)
Frame = +1
Query: 268 KRLIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 444
KRL GR ++D ++ + ++ G KV Y E+ F ++ + TK+K
Sbjct: 72 KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131
Query: 445 TAEAYLGKTVQNAVITVPAYFMTLK 519
AEA L +V + VI++PA+F ++
Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQ 156
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 43.2 bits (97), Expect = 4e-05
Identities = 18/59 (30%), Positives = 32/59 (54%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTDRLIGDAAKNQVAMNPNN 253
+GI G S + + GK +++AN++GNR PS +++ G A+ Q+ N +N
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADN 85
Score = 33.1 bits (72), Expect = 0.042
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = +1
Query: 385 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDK 525
++K E S L ++ E+AE +LG V V++VP YF + K
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRK 180
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 29.1 bits (62), Expect = 0.69
Identities = 32/154 (20%), Positives = 64/154 (41%), Gaps = 11/154 (7%)
Frame = -1
Query: 665 TAAEVKDKYISFSSTLFVKTVSNRSSSGFIDDSENVQA*DGTCIFCGLSLRVMKYAGTVI 486
+++ + + S SS L T S S+S I S + F S + I
Sbjct: 565 SSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSST------SSTFSSASTSSPSSISSSI 618
Query: 485 TAFCTVLPR*ASAVSFIFVSTMELTSSGKKVLSSPL-----------YATLILGLPPSLT 339
++ T+L + S + +S+ + S +LSS + Y++ ++ +L
Sbjct: 619 SSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSISTIPISSSLSTYSSSVIPSSSTLV 678
Query: 338 TSKGQCFMSACTVASSNLRPMRRLASNICCWGSS 237
+S +S+ VASS+ P+ +S + + +S
Sbjct: 679 SSSSSLIVSSSPVASSSSSPIPSSSSLVSTYSAS 712
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 28.7 bits (61), Expect = 0.91
Identities = 20/67 (29%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTDRLIGDAAKNQVAMNPNN 253
+ ID GT ++ + + G +EI+ R S VAF +R+ G A N P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82
Query: 254 KYSMPNV 274
+S+ NV
Sbjct: 83 AHSIRNV 89
Score = 26.2 bits (55), Expect = 4.8
Identities = 19/94 (20%), Positives = 41/94 (43%), Gaps = 2/94 (2%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRK-FEDATVQADMKHWPFEVVSDGGKPKIKVAYK-GEDKTFFPEEVS 417
P I + K L+ E VQ +P + + + +++ +++ + EE+
Sbjct: 82 PAHSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVISDEENYSLEEII 141
Query: 418 SMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLK 519
+M + AE + + + V+TVP +F L+
Sbjct: 142 AMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQ 175
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.91
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3
Query: 69 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 164
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 26.2 bits (55), Expect = 4.8
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +2
Query: 173 PSYVAFTDTDRLIGDAAKNQVAMNPNNKYSMPNVSSDVSSKMLLC 307
P +A T +R++ AAK A+N Y++ ++ D K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.4 bits (53), Expect = 8.5
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -1
Query: 155 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 51
G W SP S + H ++++ +F +RVL C+
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,169,556
Number of Sequences: 5004
Number of extensions: 67335
Number of successful extensions: 265
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 237
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 260
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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