Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021261
(705 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to ... 70 1e-12
At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to ... 70 1e-12
At1g03445.1 68414.m00325 kelch repeat-containing protein / serin... 34 0.11
At4g18640.1 68417.m02759 leucine-rich repeat transmembrane prote... 30 1.7
At5g49430.1 68418.m06116 transducin family protein / WD-40 repea... 29 2.3
At5g33200.1 68418.m03919 hypothetical protein predicted replicat... 29 4.0
At5g22920.1 68418.m02680 zinc finger (C3HC4-type RING finger) fa... 28 5.2
At5g35210.2 68418.m04175 peptidase M50 family protein / sterol-r... 27 9.2
At5g35210.1 68418.m04174 peptidase M50 family protein / sterol-r... 27 9.2
At5g08160.2 68418.m00953 serine/threonine protein kinase, putati... 27 9.2
At5g08160.1 68418.m00952 serine/threonine protein kinase, putati... 27 9.2
>At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to
nuclear protein ZAP, Mus musculus, EMBL:AB033168 this
cDNA provides a truncated ORF likely due to a skipped
exon. An alternative ORF is provided.
Length = 383
Score = 70.1 bits (164), Expect = 1e-12
Identities = 41/85 (48%), Positives = 60/85 (70%), Gaps = 8/85 (9%)
Frame = +2
Query: 2 LIRDKEAEHGGTA-RIMSIDDYFMQEGE-VEERDPTT---GKT---IKKPTLKYEFDKDC 157
L+RD E E+GG+A RI S+DDYFM E E VEE D T+ G++ I K ++Y ++ +
Sbjct: 295 LLRDVEVENGGSAPRIHSMDDYFMTEVEKVEESDSTSLSSGRSKRPIVKTVMEYCYEPEM 354
Query: 158 EESYVNSLKRAFKRSITDGYFSFLI 232
EE+Y +S+ +AFKR++ DG FSF+I
Sbjct: 355 EEAYRSSMLKAFKRTLEDGAFSFVI 379
>At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to
nuclear protein ZAP, Mus musculus, EMBL:AB033168 this
cDNA provides a truncated ORF likely due to a skipped
exon. An alternative ORF is provided.
Length = 661
Score = 70.1 bits (164), Expect = 1e-12
Identities = 41/85 (48%), Positives = 60/85 (70%), Gaps = 8/85 (9%)
Frame = +2
Query: 2 LIRDKEAEHGGTA-RIMSIDDYFMQEGE-VEERDPTT---GKT---IKKPTLKYEFDKDC 157
L+RD E E+GG+A RI S+DDYFM E E VEE D T+ G++ I K ++Y ++ +
Sbjct: 295 LLRDVEVENGGSAPRIHSMDDYFMTEVEKVEESDSTSLSSGRSKRPIVKTVMEYCYEPEM 354
Query: 158 EESYVNSLKRAFKRSITDGYFSFLI 232
EE+Y +S+ +AFKR++ DG FSF+I
Sbjct: 355 EEAYRSSMLKAFKRTLEDGAFSFVI 379
Score = 40.7 bits (91), Expect = 0.001
Identities = 16/59 (27%), Positives = 33/59 (55%), Gaps = 1/59 (1%)
Frame = +1
Query: 262 YADIWNFARQNGFQVYVCTMEL-DPQVCYKRNIHNRTLEDIEIICTRFFPTPSHHIQLD 435
+ W A+++G++ Y+ DP C RN+H T++ ++ + ++ PS ++QLD
Sbjct: 407 FTQFWATAKRSGYEAYILEATYKDPTGCAARNVHGITVDQVQQMAEQWEEAPSLYMQLD 465
>At1g03445.1 68414.m00325 kelch repeat-containing protein /
serine/threonine phosphoesterase family protein contains
Pfam profiles: PF00149 calcineurin-like phosphoesterase,
PF01344: kelch motif
Length = 795
Score = 33.9 bits (74), Expect = 0.11
Identities = 18/67 (26%), Positives = 29/67 (43%)
Frame = +2
Query: 17 EAEHGGTARIMSIDDYFMQEGEVEERDPTTGKTIKKPTLKYEFDKDCEESYVNSLKRAFK 196
E E M +DDY M E + +P I + T + D+ E + +++ AF
Sbjct: 407 EPEEDNPDNYMLLDDYLMDEPKPLSSEPEASSFIMRSTSEIAMDRLAEAHNLPTIENAFY 466
Query: 197 RSITDGY 217
S +GY
Sbjct: 467 DSAIEGY 473
>At4g18640.1 68417.m02759 leucine-rich repeat transmembrane protein
kinase, putative
Length = 686
Score = 29.9 bits (64), Expect = 1.7
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 541 TSKPLRPSQLSMEDYL-QLDDWTPNKATPXKKTVRWADIE 657
T + RPS + + L Q+ + TP KATP + WA++E
Sbjct: 640 TEQRQRPSMKDVAEQLKQVINITPEKATPRSSPLWWAELE 679
>At5g49430.1 68418.m06116 transducin family protein / WD-40 repeat
family protein similar to WD-repeat protein 9 (SP:Q9NSI6)
{Homo sapiens}; contains Pfam PF00400: WD domain, G-beta
repeat (4 copies)
Length = 1677
Score = 29.5 bits (63), Expect = 2.3
Identities = 19/79 (24%), Positives = 35/79 (44%), Gaps = 11/79 (13%)
Frame = +3
Query: 375 RHRNYMY--TVLPDAESPHTTGSDHVTAERGHYGCSHGRRADEV---------IAEIHEK 521
+H+N + L SP + DH + + H G S+G E+ + I +K
Sbjct: 1261 KHKNNLDRDAALTKEVSPGSCSQDHGSGSKSHEGASNGFHGTELNGLEKSEGSLTHIQDK 1320
Query: 522 LSETRRNQQTASAITTLHG 578
+S++R NQ + + + G
Sbjct: 1321 ISDSRGNQNSQEELRSASG 1339
>At5g33200.1 68418.m03919 hypothetical protein predicted replication
A1 proteins - Arabidopsis thaliana
Length = 426
Score = 28.7 bits (61), Expect = 4.0
Identities = 15/54 (27%), Positives = 30/54 (55%), Gaps = 3/54 (5%)
Frame = +3
Query: 480 GRRADEVIAEIHEKLSETR---RNQQTASAITTLHGRLPTARRLDAEQGDAXKE 632
G DEV+ ++ ++ R+Q+T+ ++T L R+ T + D+EQ + K+
Sbjct: 346 GLCGDEVLEDLEYSVNPASIISRDQETSDSVTPLSKRVYTTKLSDSEQSSSSKK 399
>At5g22920.1 68418.m02680 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profiles:PF05495 CHY zinc
finger, PF00097 zinc finger, C3HC4 type (RING finger)
Length = 291
Score = 28.3 bits (60), Expect = 5.2
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 459 GHYGCSHGRRADEVIAEIHEKLSETR 536
GHYGCSH RR ++ A +++ + R
Sbjct: 23 GHYGCSHYRRRCKIRAPCCDEIFDCR 48
>At5g35210.2 68418.m04175 peptidase M50 family protein /
sterol-regulatory element binding protein (SREBP) site 2
protease family protein contains PFam PF02163:
sterol-regulatory element binding protein (SREBP) site 2
protease
Length = 1409
Score = 27.5 bits (58), Expect = 9.2
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +1
Query: 259 HYADIWNFARQNGFQVYVCTMELD 330
H ++ F R FQ+Y+C EL+
Sbjct: 197 HLLSVYGFLRSFSFQLYICPFELN 220
>At5g35210.1 68418.m04174 peptidase M50 family protein /
sterol-regulatory element binding protein (SREBP) site 2
protease family protein contains PFam PF02163:
sterol-regulatory element binding protein (SREBP) site 2
protease
Length = 1576
Score = 27.5 bits (58), Expect = 9.2
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +1
Query: 259 HYADIWNFARQNGFQVYVCTMELD 330
H ++ F R FQ+Y+C EL+
Sbjct: 197 HLLSVYGFLRSFSFQLYICPFELN 220
>At5g08160.2 68418.m00953 serine/threonine protein kinase, putative
identical to serine/threonine protein kinase
[Arabidopsis thaliana] gi|2109293|gb|AAB69123
Length = 311
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 6/48 (12%)
Frame = +1
Query: 538 GTSKPLRPSQLSMEDYLQLDDWTPNK-ATPXKKTVRW-----ADIEER 663
G+++P R S ++ LQL +WT + P + W ADI+ER
Sbjct: 175 GSARPSRKQIRSRQEALQLQEWTSEHCSAPFRAPELWDCPSHADIDER 222
>At5g08160.1 68418.m00952 serine/threonine protein kinase, putative
identical to serine/threonine protein kinase
[Arabidopsis thaliana] gi|2109293|gb|AAB69123
Length = 347
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 6/48 (12%)
Frame = +1
Query: 538 GTSKPLRPSQLSMEDYLQLDDWTPNK-ATPXKKTVRW-----ADIEER 663
G+++P R S ++ LQL +WT + P + W ADI+ER
Sbjct: 211 GSARPSRKQIRSRQEALQLQEWTSEHCSAPFRAPELWDCPSHADIDER 258
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,856,478
Number of Sequences: 28952
Number of extensions: 299994
Number of successful extensions: 884
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 846
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 880
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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