Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021257
(844 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g16270.1 68418.m01900 Rad21/Rec8-like family protein weak sim... 34 0.10
At3g14400.1 68416.m01822 ubiquitin-specific protease 25 (UBP25) ... 30 1.7
At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kina... 30 1.7
At5g27240.1 68418.m03249 DNAJ heat shock N-terminal domain-conta... 30 2.2
At3g11020.1 68416.m01330 DRE-binding protein (DREB2B) identical ... 29 3.9
At3g13225.1 68416.m01660 WW domain-containing protein contains P... 29 5.1
At2g22890.1 68415.m02717 expressed protein ; expression support... 29 5.1
At1g71740.1 68414.m08292 hypothetical protein 29 5.1
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 29 5.1
At1g50770.1 68414.m05710 hypothetical protein 28 6.8
At5g04950.1 68418.m00524 nicotianamine synthase, putative simila... 28 8.9
At1g68790.1 68414.m07863 expressed protein 28 8.9
>At5g16270.1 68418.m01900 Rad21/Rec8-like family protein weak
similarity to cohesion family protein SYN2 [Arabidopsis
thaliana] GI:12006360; contains Pfam profiles PF04824:
Conserved region of Rad21 / Rec8 like protein, PF04825:
N terminus of Rad21 / Rec8 like protein; supporting cDNA
gi|18157648|gb|AF400129.1|AF400129
Length = 1031
Score = 34.3 bits (75), Expect = 0.10
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = +3
Query: 294 PEDTHRVKIEPHSQPSGEDSASDLGVDGIKTEIDGLMDSDGKSD 425
PE K EPH++ S E+++ +DG T DGLM + + D
Sbjct: 867 PETESYNKYEPHNEMSNEEASMQNALDGEHTSRDGLMGDNDEMD 910
>At3g14400.1 68416.m01822 ubiquitin-specific protease 25 (UBP25)
similar to GI:11993490
Length = 661
Score = 30.3 bits (65), Expect = 1.7
Identities = 19/82 (23%), Positives = 36/82 (43%), Gaps = 3/82 (3%)
Frame = +3
Query: 306 HRVKIEPHSQPSGEDSASDLGVDGIKTEIDGLMDSDGKSDPTKSPSCELGGPGSLKSERL 485
H+V + PH + +G + D D + S+G + T P E G + +S+ L
Sbjct: 501 HKVALHPHERSNGSSNGGDHHKDNLHP-----CGSNGSQNGTAHPETERNGVSTTQSKGL 555
Query: 486 SSDSND---ILIHRQASEARQI 542
S + + IL+ + S ++
Sbjct: 556 CSSTKEDPCILLRKDESSRNEL 577
>At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 769
Score = 30.3 bits (65), Expect = 1.7
Identities = 18/44 (40%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = -1
Query: 517 LWIKMSFESEDSLSDFNEP-GPPSSQEGDFVGSLLPSLSMRPSI 389
L + + F + D L+D EP S QE D VGS+ +L PSI
Sbjct: 587 LLLGLHFRAPDPLTDILEPPNEMSDQESDSVGSVDVNLPREPSI 630
>At5g27240.1 68418.m03249 DNAJ heat shock N-terminal
domain-containing protein
Length = 1104
Score = 29.9 bits (64), Expect = 2.2
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +3
Query: 285 KKEPEDTHRVKIEPHSQPSGEDSASDLGVDGIKTEIDGLMDSDGKSDP-TKSPS 443
+K P+D H+ + ++S S DG+ T+++G M S S+P T+ PS
Sbjct: 834 RKTPQDIHKPTVNVKKHGRNDESLSQSRGDGLLTQLNGNMKS---SEPETRVPS 884
>At3g11020.1 68416.m01330 DRE-binding protein (DREB2B) identical to
DREB2B GI:3738232 from [Arabidopsis thaliana]; supported
by cDNA:gi_3738231_dbj_AB007791.1_AB007791
Length = 330
Score = 29.1 bits (62), Expect = 3.9
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -1
Query: 499 FESEDSLSDFNEPGPPSSQEGDFVGS 422
F+ + L D NEPGP SQ+ + V S
Sbjct: 279 FDINELLGDLNEPGPHQSQDQNHVNS 304
>At3g13225.1 68416.m01660 WW domain-containing protein contains Pfam
profile PF00397: WW domain
Length = 863
Score = 28.7 bits (61), Expect = 5.1
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +3
Query: 309 RVKIEPHSQPSGEDSASDLGVDGIKTEIDGLMDSDGKSDPTKSPS 443
RVK++ ++PSG+ + + L + G ++ + DSD K+D T + S
Sbjct: 18 RVKLDLFAEPSGQKTENPLLLLGQYSDDEVEEDSDKKADDTSADS 62
>At2g22890.1 68415.m02717 expressed protein ; expression supported
by MPSS
Length = 279
Score = 28.7 bits (61), Expect = 5.1
Identities = 17/37 (45%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -1
Query: 607 STRTHVIW-AIWITTVWVSILKIICRASEACLWIKMS 500
ST TH +W A TTV+VS K I A + LW++ S
Sbjct: 62 STWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPS 98
>At1g71740.1 68414.m08292 hypothetical protein
Length = 130
Score = 28.7 bits (61), Expect = 5.1
Identities = 18/42 (42%), Positives = 27/42 (64%), Gaps = 3/42 (7%)
Frame = +2
Query: 197 PASRTSPVTL--LSLATVPVRL-GVAAHSGGAEERTRRHASC 313
P SRT P+T+ + LA+ VRL +A+HS + + RR A+C
Sbjct: 6 PPSRTKPLTIFKMGLASASVRLVNLASHS---KRKLRRWAAC 44
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 28.7 bits (61), Expect = 5.1
Identities = 20/48 (41%), Positives = 24/48 (50%)
Frame = -1
Query: 484 SLSDFNEPGPPSSQEGDFVGSLLPSLSMRPSISVLMPSTPRSDAESSP 341
SLS + P PP S + SL PSLS P S + P S + SSP
Sbjct: 62 SLSPSSPPPPPPSSSP--LSSLSPSLSPSPPSSSPSSAPPSSLSPSSP 107
>At1g50770.1 68414.m05710 hypothetical protein
Length = 632
Score = 28.3 bits (60), Expect = 6.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3
Query: 420 SDPTKSPSCELGGPGSLKSERLSSDSN 500
+D +K P C L GS+ E+ SSD N
Sbjct: 496 NDGSKEPDCLLHEDGSIAGEKTSSDEN 522
>At5g04950.1 68418.m00524 nicotianamine synthase, putative similar
to nicotianamine synthase [Lycopersicon
esculentum][GI:4753801], nicotianamine synthase 2
[Hordeum vulgare][GI:4894912]
Length = 320
Score = 27.9 bits (59), Expect = 8.9
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -3
Query: 623 LHLPPFYTNPCYLGHLDYDSLGFH 552
LH+ P+Y+N LG L++D L H
Sbjct: 97 LHIFPYYSNYLKLGKLEFDLLSQH 120
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 27.9 bits (59), Expect = 8.9
Identities = 13/48 (27%), Positives = 25/48 (52%)
Frame = +3
Query: 378 IKTEIDGLMDSDGKSDPTKSPSCELGGPGSLKSERLSSDSNDILIHRQ 521
++TE +++ GK+ P + P E+GG G ++ D N +I +
Sbjct: 1011 VETETVFKVNNTGKN-PVEDPQLEVGGSGEIREHGEEDDENISMIEEE 1057
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,716,477
Number of Sequences: 28952
Number of extensions: 392904
Number of successful extensions: 1104
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1061
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1104
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1950880000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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