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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021255
         (760 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual        38   0.002
SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineur...    32   0.10 
SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc...    27   3.8  
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac...    27   3.8  
SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein Klp6|S...    27   3.8  
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom...    26   6.7  
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo...    25   8.9  
SPBC776.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    25   8.9  

>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1279

 Score = 37.9 bits (84), Expect = 0.002
 Identities = 13/50 (26%), Positives = 30/50 (60%)
 Frame = +3

Query: 519  QILTHFQQQASKQISIPKEHYRWILGKQGQKLKELEKVTATKINVPGISD 668
            +I+   + Q  ++I +P+     I+G+ G   +++E+ T+T +N+P + D
Sbjct: 1030 EIVEELKNQVEEKIEVPQRCISSIIGRMGSTRRDIERKTSTMLNIPNVLD 1079



 Score = 34.7 bits (76), Expect = 0.014
 Identities = 20/63 (31%), Positives = 30/63 (47%)
 Frame = +3

Query: 468 SNFPYHWKAERCSRARRQILTHFQQQASKQISIPKEHYRWILGKQGQKLKELEKVTATKI 647
           + F    K      ARRQIL    ++ +K +  P      I+G  GQ LK +   T+T+I
Sbjct: 199 TTFLIQGKTSAVKAARRQILKLIGRRETKTMPCPVFVVGAIIGTNGQNLKSIMDRTSTRI 258

Query: 648 NVP 656
            +P
Sbjct: 259 QIP 261



 Score = 27.9 bits (59), Expect = 1.7
 Identities = 11/33 (33%), Positives = 19/33 (57%)
 Frame = +3

Query: 558  ISIPKEHYRWILGKQGQKLKELEKVTATKINVP 656
            + IP   +R I+G  G  + ++ K+   KI+VP
Sbjct: 1212 LGIPTNLHRRIIGSGGSIINKIRKIAQVKIDVP 1244



 Score = 27.5 bits (58), Expect = 2.2
 Identities = 15/32 (46%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
 Frame = +1

Query: 412 SITKDTGAHIEIST-SKDGSLTFLITGKQSAV 504
           ++   T   I +ST SK  + TFLI GK SAV
Sbjct: 179 TVMHQTSTRINVSTASKTKNTTFLIQGKTSAV 210


>SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineurin
           deletion Rnc1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 398

 Score = 31.9 bits (69), Expect = 0.10
 Identities = 12/39 (30%), Positives = 25/39 (64%)
 Frame = +3

Query: 537 QQQASKQISIPKEHYRWILGKQGQKLKELEKVTATKINV 653
           Q + ++ ISIP +    I+G+ G K+ E+ + + +KI++
Sbjct: 318 QPKVTQNISIPADMVGCIIGRGGSKISEIRRTSGSKISI 356


>SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit
           Apc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1458

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 8/20 (40%), Positives = 16/20 (80%)
 Frame = +3

Query: 582 RWILGKQGQKLKELEKVTAT 641
           +W+  KQ Q++KE++ +T+T
Sbjct: 657 KWVFNKQDQEVKEIKALTST 676


>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
            Spp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2363

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 11/33 (33%), Positives = 17/33 (51%)
 Frame = -3

Query: 182  QHAWVHQDERVSHLQPSWIADASFLSPPAVLVR 84
            Q+ W   D R  HL PSW+  +    PP ++ +
Sbjct: 947  QYLWFEADRR--HLFPSWVKPSDSEPPPLLVYK 977


>SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein
           Klp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 784

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 11/24 (45%), Positives = 13/24 (54%)
 Frame = +1

Query: 337 HVPYEERKLDNANTFGEGESLRTC 408
           HVPY + KL     F  G + RTC
Sbjct: 338 HVPYRDSKLTRLLKFSLGGNCRTC 361


>SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1462

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 12/42 (28%), Positives = 22/42 (52%)
 Frame = +2

Query: 113  MMHQQSMMVGDVIPVHPDVPMHVEEMNNVGYENNVSFAYDDL 238
            ++H    + G  I V P++P H+ +  N  + NN+   Y +L
Sbjct: 1396 ILHGAGALAGPTIEVDPEIPDHLIKTWNTTW-NNLFIYYPEL 1436


>SPCC63.04 |mok14||alpha-1,3-glucan synthase
           Mok14|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1369

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
 Frame = +2

Query: 137 VGDVI-PVHPDVPMHVEEMNNVGYENNVSFAYDDLFPALPLPSPL 268
           VGD+  PV    P  V ++ N  YE  V + Y D    + L SP+
Sbjct: 301 VGDITYPVAETAPSLVVKVVNQDYEVKVFYHYKDNIKYVLLDSPI 345


>SPBC776.16 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 253

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 9/24 (37%), Positives = 15/24 (62%)
 Frame = +2

Query: 188 MNNVGYENNVSFAYDDLFPALPLP 259
           +NN   E+ +   ++ LF +LPLP
Sbjct: 11  INNAPKEDRIQVKFEQLFESLPLP 34


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,015,298
Number of Sequences: 5004
Number of extensions: 63383
Number of successful extensions: 179
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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