Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021252X
(543 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g56440.1 68416.m06277 WD-40 repeat protein family contains 4 ... 38 0.004
At2g40810.2 68415.m05035 WD-40 repeat protein family similar to ... 36 0.023
At2g40810.1 68415.m05034 WD-40 repeat protein family similar to ... 36 0.023
At3g62770.2 68416.m07051 transport protein-related weak similari... 34 0.071
At3g62770.1 68416.m07052 transport protein-related weak similari... 34 0.071
At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p... 31 0.38
At3g11960.2 68416.m01476 cleavage and polyadenylation specificit... 30 0.87
At3g11960.1 68416.m01475 cleavage and polyadenylation specificit... 30 0.87
At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p... 30 0.87
At5g23430.2 68418.m02749 transducin family protein / WD-40 repea... 29 2.0
At5g23430.1 68418.m02748 transducin family protein / WD-40 repea... 29 2.0
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 28 3.5
At5g37370.2 68418.m04490 pre-mRNA splicing factor PRP38 family p... 28 4.6
At5g37370.1 68418.m04489 pre-mRNA splicing factor PRP38 family p... 28 4.6
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea... 28 4.6
At3g01720.1 68416.m00107 expressed protein 27 6.1
At4g35920.3 68417.m05107 expressed protein contains Pfam profile... 27 8.1
At4g35920.2 68417.m05106 expressed protein contains Pfam profile... 27 8.1
At4g35920.1 68417.m05105 expressed protein contains Pfam profile... 27 8.1
>At3g56440.1 68416.m06277 WD-40 repeat protein family contains 4
WD-40 repeats (PF00400) (2 weak); PS00778 Histidine acid
phosphatases active site signature; similar to Gsa12p
(GI:18307769) {Pichia pastoris}similar to
uncharacterized protein JM5 (GP:3114828) [Homo sapiens]
Length = 391
Score = 37.9 bits (84), Expect = 0.004
Identities = 16/43 (37%), Positives = 21/43 (48%)
Frame = +3
Query: 81 GVEDMARRKSSGITSLSFNQDQGCFTCCLKTGLRVYNVEPLVE 209
G D + + S+S+NQD CF G R+YN EP E
Sbjct: 24 GPPDFGESDEAELVSVSWNQDYSCFAAGTSHGFRIYNCEPFKE 66
Score = 32.3 bits (70), Expect = 0.22
Identities = 19/55 (34%), Positives = 35/55 (63%), Gaps = 5/55 (9%)
Frame = +2
Query: 251 MQMVFRTNWLLVV----KARKPYSLLLL-DDQQRAFKAEVVFKSPIRALRARKDK 400
++M+FR+N L +V ++ P + +L+ DD Q +E F+S IRA++ R+D+
Sbjct: 80 VEMLFRSNILALVGGGPNSQYPSNKVLIWDDHQGRCISEFTFRSEIRAVKLRRDR 134
>At2g40810.2 68415.m05035 WD-40 repeat protein family similar to
Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam
PF00400: WD domain, G-beta repeats
Length = 393
Score = 35.5 bits (78), Expect = 0.023
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 99 RRKSSGITSLSFNQDQGCFTCCLKTGLRVYNVEPLVE 209
+++ + + S+ +NQD CF G R+YN EP E
Sbjct: 26 KKEEAELVSVCWNQDSSCFAAGTSHGFRIYNCEPFKE 62
Score = 33.9 bits (74), Expect = 0.071
Identities = 20/55 (36%), Positives = 35/55 (63%), Gaps = 5/55 (9%)
Frame = +2
Query: 251 MQMVFRTNWLLVV----KARKPYSLLLL-DDQQRAFKAEVVFKSPIRALRARKDK 400
++M+FR+N L +V ++ P S +L+ DD Q +E F+S IRA++ R+D+
Sbjct: 76 VEMLFRSNILALVGGGPNSQYPSSKVLIWDDHQSRCISEFAFRSEIRAVKLRRDR 130
>At2g40810.1 68415.m05034 WD-40 repeat protein family similar to
Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam
PF00400: WD domain, G-beta repeats
Length = 393
Score = 35.5 bits (78), Expect = 0.023
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 99 RRKSSGITSLSFNQDQGCFTCCLKTGLRVYNVEPLVE 209
+++ + + S+ +NQD CF G R+YN EP E
Sbjct: 26 KKEEAELVSVCWNQDSSCFAAGTSHGFRIYNCEPFKE 62
Score = 33.9 bits (74), Expect = 0.071
Identities = 20/55 (36%), Positives = 35/55 (63%), Gaps = 5/55 (9%)
Frame = +2
Query: 251 MQMVFRTNWLLVV----KARKPYSLLLL-DDQQRAFKAEVVFKSPIRALRARKDK 400
++M+FR+N L +V ++ P S +L+ DD Q +E F+S IRA++ R+D+
Sbjct: 76 VEMLFRSNILALVGGGPNSQYPSSKVLIWDDHQSRCISEFAFRSEIRAVKLRRDR 130
>At3g62770.2 68416.m07051 transport protein-related weak similarity
to Gsa12p [Pichia pastoris] GI:18307769; contains 1
WD-40 repeat (PF00400); putative proteins - different
species
Length = 432
Score = 33.9 bits (74), Expect = 0.071
Identities = 21/63 (33%), Positives = 35/63 (55%), Gaps = 5/63 (7%)
Frame = +2
Query: 227 RGAGRGVPMQMVFRTNWLLVVKARK-----PYSLLLLDDQQRAFKAEVVFKSPIRALRAR 391
RG G V ++M+FR N L +V P +++ DD Q E+ F+S +R++R R
Sbjct: 116 RGGGVAV-VEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQGRCIGELSFRSDVRSVRLR 174
Query: 392 KDK 400
+D+
Sbjct: 175 RDR 177
Score = 31.9 bits (69), Expect = 0.28
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +3
Query: 117 ITSLSFNQDQGCFTCCLKTGLRVYNVEPLVE 209
+ LSFNQD CF G R+ N +P E
Sbjct: 78 VLHLSFNQDHACFAVGTDRGFRILNCDPFRE 108
>At3g62770.1 68416.m07052 transport protein-related weak similarity
to Gsa12p [Pichia pastoris] GI:18307769; contains 1
WD-40 repeat (PF00400); putative proteins - different
species
Length = 307
Score = 33.9 bits (74), Expect = 0.071
Identities = 21/63 (33%), Positives = 35/63 (55%), Gaps = 5/63 (7%)
Frame = +2
Query: 227 RGAGRGVPMQMVFRTNWLLVVKARK-----PYSLLLLDDQQRAFKAEVVFKSPIRALRAR 391
RG G V ++M+FR N L +V P +++ DD Q E+ F+S +R++R R
Sbjct: 116 RGGGVAV-VEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQGRCIGELSFRSDVRSVRLR 174
Query: 392 KDK 400
+D+
Sbjct: 175 RDR 177
Score = 31.9 bits (69), Expect = 0.28
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +3
Query: 117 ITSLSFNQDQGCFTCCLKTGLRVYNVEPLVE 209
+ LSFNQD CF G R+ N +P E
Sbjct: 78 VLHLSFNQDHACFAVGTDRGFRILNCDPFRE 108
>At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p80
subunit, putative similar to contains 6 WD-40 repeats
(PF00400); katanin p80 subunit (GI:3005601)
[Strongylocentrotus purpuratus]
Length = 974
Score = 31.5 bits (68), Expect = 0.38
Identities = 12/36 (33%), Positives = 24/36 (66%)
Frame = +3
Query: 99 RRKSSGITSLSFNQDQGCFTCCLKTGLRVYNVEPLV 206
R +++G+ +++F+ D C L GL+VY+ EP++
Sbjct: 172 RPEATGVRAIAFHPDGQTLFCGLDDGLKVYSWEPVI 207
>At3g11960.2 68416.m01476 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1329
Score = 30.3 bits (65), Expect = 0.87
Identities = 17/51 (33%), Positives = 21/51 (41%)
Frame = +3
Query: 135 NQDQGCFTCCLKTGLRVYNVEPLVEKAHYSKEELGEVSLCRWCSAPTGYSW 287
N D G T C K P + Y+ E L SL CS+P YS+
Sbjct: 952 NSDSGSMTICSKACSSSQRTSPFHDVVGYTTENLSSSSL---CSSPDDYSY 999
>At3g11960.1 68416.m01475 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1379
Score = 30.3 bits (65), Expect = 0.87
Identities = 17/51 (33%), Positives = 21/51 (41%)
Frame = +3
Query: 135 NQDQGCFTCCLKTGLRVYNVEPLVEKAHYSKEELGEVSLCRWCSAPTGYSW 287
N D G T C K P + Y+ E L SL CS+P YS+
Sbjct: 1010 NSDSGSMTICSKACSSSQRTSPFHDVVGYTTENLSSSSL---CSSPDDYSY 1057
>At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p80
subunit, putative contains 5 WD-40 repeats (PF00400);
similar to katanin p80 subunit (GI:3005601)
[Strongylocentrotus purpuratus]
Length = 1180
Score = 30.3 bits (65), Expect = 0.87
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = +3
Query: 99 RRKSSGITSLSFNQDQGCFTCCLKTGLRVYNVEPLV 206
R +++G+ S+ F+ D C L L+VY+ EP+V
Sbjct: 223 RPEATGVRSIKFHPDGRTLFCGLDDSLKVYSWEPVV 258
>At5g23430.2 68418.m02749 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400);
katanin p80 subunit (GI:3005601) [Strongylocentrotus
purpuratus]
Length = 836
Score = 29.1 bits (62), Expect = 2.0
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +3
Query: 105 KSSGITSLSFNQDQGCFTCCLKTGLRVYNVEPL 203
+++G+ LSFN D C L+ L++++ EP+
Sbjct: 226 ETAGVRCLSFNPDGKTVLCGLQESLKIFSWEPI 258
>At5g23430.1 68418.m02748 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400);
katanin p80 subunit (GI:3005601) [Strongylocentrotus
purpuratus]
Length = 837
Score = 29.1 bits (62), Expect = 2.0
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +3
Query: 105 KSSGITSLSFNQDQGCFTCCLKTGLRVYNVEPL 203
+++G+ LSFN D C L+ L++++ EP+
Sbjct: 226 ETAGVRCLSFNPDGKTVLCGLQESLKIFSWEPI 258
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 28.3 bits (60), Expect = 3.5
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -1
Query: 243 PRPAPLYCNAPSRPGAPHY 187
P PAP Y ++P P PHY
Sbjct: 703 PPPAPYYYSSPQPPPPPHY 721
>At5g37370.2 68418.m04490 pre-mRNA splicing factor PRP38 family
protein (SRL1) contains Pfam profile PF03371: PRP38
family
Length = 385
Score = 27.9 bits (59), Expect = 4.6
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +1
Query: 205 SRRRITVKRSWARCPYADGVPHQLATRGESSEAVQPPPAG 324
+RR +VK S + + PH+ +TRG SS +PPP G
Sbjct: 186 ARRPPSVKASLS-VSFGQRAPHRASTRG-SSPVRRPPPTG 223
>At5g37370.1 68418.m04489 pre-mRNA splicing factor PRP38 family
protein (SRL1) contains Pfam profile PF03371: PRP38
family
Length = 393
Score = 27.9 bits (59), Expect = 4.6
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +1
Query: 205 SRRRITVKRSWARCPYADGVPHQLATRGESSEAVQPPPAG 324
+RR +VK S + + PH+ +TRG SS +PPP G
Sbjct: 194 ARRPPSVKASLS-VSFGQRAPHRASTRG-SSPVRRPPPTG 231
>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
family protein similar to katanin p80 subunit
[Strongylocentrotus purpuratus] GI:3005601; contains
Pfam profile PF00400: WD domain, G-beta repeat
Length = 871
Score = 27.9 bits (59), Expect = 4.6
Identities = 10/33 (30%), Positives = 21/33 (63%)
Frame = +3
Query: 105 KSSGITSLSFNQDQGCFTCCLKTGLRVYNVEPL 203
+++G+ L+FN D C L+ L++++ EP+
Sbjct: 319 ETTGVRCLTFNPDGKSVLCGLQESLKIFSWEPI 351
>At3g01720.1 68416.m00107 expressed protein
Length = 802
Score = 27.5 bits (58), Expect = 6.1
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = +3
Query: 195 EPLVEKAHYSKEELGEVSLCRWCSAPTGYSW 287
E +K HY KE G++ W S GYS+
Sbjct: 565 EVRADKEHYGKELTGDIYESGWISEMYGYSF 595
>At4g35920.3 68417.m05107 expressed protein contains Pfam profile
PF04749: Protein of unknown function, DUF614; isoform
contains AT-acceptor splice site at intron 8
Length = 421
Score = 27.1 bits (57), Expect = 8.1
Identities = 11/36 (30%), Positives = 17/36 (47%)
Frame = +2
Query: 20 SENVQSENIQNIKSMFMIISRRRRHGAPEEQWHNEL 127
S S + N+ S ++SRR E+WH +L
Sbjct: 269 STRAASRSTSNVSSGHDLLSRRASQAQHHEEWHTDL 304
>At4g35920.2 68417.m05106 expressed protein contains Pfam profile
PF04749: Protein of unknown function, DUF614; isoform
contains AT-acceptor splice site at intron 8
Length = 421
Score = 27.1 bits (57), Expect = 8.1
Identities = 11/36 (30%), Positives = 17/36 (47%)
Frame = +2
Query: 20 SENVQSENIQNIKSMFMIISRRRRHGAPEEQWHNEL 127
S S + N+ S ++SRR E+WH +L
Sbjct: 269 STRAASRSTSNVSSGHDLLSRRASQAQHHEEWHTDL 304
>At4g35920.1 68417.m05105 expressed protein contains Pfam profile
PF04749: Protein of unknown function, DUF614; isoform
contains AT-acceptor splice site at intron 8
Length = 421
Score = 27.1 bits (57), Expect = 8.1
Identities = 11/36 (30%), Positives = 17/36 (47%)
Frame = +2
Query: 20 SENVQSENIQNIKSMFMIISRRRRHGAPEEQWHNEL 127
S S + N+ S ++SRR E+WH +L
Sbjct: 269 STRAASRSTSNVSSGHDLLSRRASQAQHHEEWHTDL 304
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,566,153
Number of Sequences: 28952
Number of extensions: 259250
Number of successful extensions: 749
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 719
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 749
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1013649368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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