Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021244
(708 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g27880.1 68415.m03380 argonaute protein, putative / AGO, puta... 66 2e-11
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 63 2e-10
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 63 2e-10
At5g43810.1 68418.m05357 pinhead protein (PINHEAD) / zwille prot... 56 3e-08
At2g27040.1 68415.m03249 PAZ domain-containing protein / piwi do... 46 2e-05
At2g32940.1 68415.m04038 PAZ domain-containing protein / piwi do... 46 2e-05
At1g69440.1 68414.m07979 PAZ domain-containing protein / piwi do... 43 2e-04
At5g21150.1 68418.m02526 PAZ domain-containing protein / piwi do... 40 0.001
At5g21030.1 68418.m02501 PAZ domain-containing protein / piwi do... 40 0.001
At1g31280.1 68414.m03828 PAZ domain-containing protein / piwi do... 40 0.001
At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi do... 39 0.004
At1g31730.1 68414.m03893 epsilon-adaptin, putative similar to SP... 29 4.0
At5g60740.1 68418.m07621 ABC transporter family protein similar ... 28 7.0
>At2g27880.1 68415.m03380 argonaute protein, putative / AGO,
putative similar to SP|O04379 Argonaute protein (AGO1)
{Arabidopsis thaliana}; contains Pfam profiles PF02170:
PAZ domain, PF02171: Piwi domain
Length = 997
Score = 66.1 bits (154), Expect = 2e-11
Identities = 28/82 (34%), Positives = 49/82 (59%)
Frame = +1
Query: 10 TVYEYFMKEKKYRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTMVR 189
TV +YF ++ YR+KY L + G + +YLPMEL ++ GQ K+LN++Q++ +++
Sbjct: 430 TVVQYFAEKYNYRVKYQALPAIQTGSDTRPVYLPMELCQIDEGQRYTKRLNEKQVTALLK 489
Query: 190 EAATPPDVRKRKIEEVIQKMNY 255
P R+ I+ ++ K NY
Sbjct: 490 ATCQRPPDRENSIKNLVVKNNY 511
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 62.9 bits (146), Expect = 2e-10
Identities = 29/86 (33%), Positives = 53/86 (61%)
Frame = +1
Query: 10 TVYEYFMKEKKYRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTMVR 189
+V EYF + +RI++ L CL VG ++ YLPME+ ++ GQ +K+LN+RQ++ +++
Sbjct: 462 SVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEVCKIVEGQRYSKRLNERQITALLK 521
Query: 190 EAATPPDVRKRKIEEVIQKMNYRRIN 267
P R++ I + +Q +Y + N
Sbjct: 522 VTCQRPIDREKDILQTVQLNDYAKDN 547
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 62.9 bits (146), Expect = 2e-10
Identities = 29/86 (33%), Positives = 53/86 (61%)
Frame = +1
Query: 10 TVYEYFMKEKKYRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTMVR 189
+V EYF + +RI++ L CL VG ++ YLPME+ ++ GQ +K+LN+RQ++ +++
Sbjct: 460 SVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEVCKIVEGQRYSKRLNERQITALLK 519
Query: 190 EAATPPDVRKRKIEEVIQKMNYRRIN 267
P R++ I + +Q +Y + N
Sbjct: 520 VTCQRPIDREKDILQTVQLNDYAKDN 545
>At5g43810.1 68418.m05357 pinhead protein (PINHEAD) / zwille protein
(ZWILLE) identical to SP|Q9XGW1 PINHEAD protein (ZWILLE
protein) {Arabidopsis thaliana}; contains Pfam profiles
PF02171: Piwi domain, PF02170: PAZ domain
Length = 988
Score = 55.6 bits (128), Expect = 3e-08
Identities = 27/82 (32%), Positives = 45/82 (54%)
Frame = +1
Query: 10 TVYEYFMKEKKYRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTMVR 189
+V EYF + + I++ L CL VG + K YLPME ++ GQ K+LN++Q++ +++
Sbjct: 410 SVIEYFQEMYGFTIQHTHLPCLQVGNQKKASYLPMEACKIVEGQRYTKRLNEKQITALLK 469
Query: 190 EAATPPDVRKRKIEEVIQKMNY 255
P R+ I +Q Y
Sbjct: 470 VTCQRPRDRENDILRTVQHNAY 491
>At2g27040.1 68415.m03249 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|Q9QZ81
Eukaryotic translation initiation factor 2C 2 {Rattus
norvegicus}; contains Pfam profiles PF02171: Piwi
domain, PF02170: PAZ domain
Length = 924
Score = 46.4 bits (105), Expect = 2e-05
Identities = 24/80 (30%), Positives = 41/80 (51%), Gaps = 2/80 (2%)
Frame = +3
Query: 273 KTYGLEIANEFYQVEAKILEAPTLEVGPRQFTVPKKGVWQANC--LLKPEALNSWGFIAI 446
++ G+ I++ F QVE ++L AP L++G T P+ G W N ++P + W + +
Sbjct: 455 RSCGISISSNFTQVEGRVLPAPKLKMGCGSETFPRNGRWNFNNKEFVEPTKIQRW--VVV 512
Query: 447 ELDPRGCNYEDIVSKLMNTG 506
R CN +V L+ G
Sbjct: 513 NFSAR-CNVRQVVDDLIKIG 531
>At2g32940.1 68415.m04038 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}, SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 878
Score = 46.0 bits (104), Expect = 2e-05
Identities = 26/87 (29%), Positives = 41/87 (47%), Gaps = 2/87 (2%)
Frame = +3
Query: 258 KNQFFKTYGLEIANEFYQVEAKILEAPTLEVGPRQFTVPKKGVWQAN--CLLKPEALNSW 431
K+ F G+ I E QVE ++L+ P L+ G + P G W N LL+P A+ SW
Sbjct: 414 KDPFLAGCGISIEKEMTQVEGRVLKPPMLKFGKNEDFQPCNGRWNFNNKMLLEPRAIKSW 473
Query: 432 GFIAIELDPRGCNYEDIVSKLMNTGRK 512
+ C+ I +L++ G +
Sbjct: 474 AIVNFSFP---CDSSHISRELISCGMR 497
Score = 39.9 bits (89), Expect = 0.002
Identities = 24/83 (28%), Positives = 37/83 (44%)
Frame = +1
Query: 7 MTVYEYFMKEKKYRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTMV 186
+TVY+YF + I CL VG D+ YLP+E + Q K L+ RQ +V
Sbjct: 330 ITVYDYFKQTYTEPISSAYFPCLDVGKPDRPNYLPLEFCNLVSLQRYTKPLSGRQRVLLV 389
Query: 187 REAATPPDVRKRKIEEVIQKMNY 255
+ P R + + + + Y
Sbjct: 390 ESSRQKPLERIKTLNDAMHTYCY 412
>At1g69440.1 68414.m07979 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 990
Score = 43.2 bits (97), Expect = 2e-04
Identities = 23/78 (29%), Positives = 42/78 (53%)
Frame = +1
Query: 7 MTVYEYFMKEKKYRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTMV 186
+ + YF Y I++ +L CL + + + YLPMEL + GQ +L+D Q + ++
Sbjct: 443 LRLMSYFKDHYGYEIQFKNLPCLQIS-RARPCYLPMELCMICEGQKFLGKLSDDQAAKIM 501
Query: 187 REAATPPDVRKRKIEEVI 240
+ P+ RK I++V+
Sbjct: 502 KMGCQKPNERKAIIDKVM 519
Score = 29.5 bits (63), Expect = 2.3
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = +2
Query: 614 LVVVVSGRGRDYYHKLKQIAELKVGILTQC 703
L++ V + Y LK+I+E ++G++TQC
Sbjct: 649 LIICVMEKKHKGYGDLKRISETRIGVVTQC 678
>At5g21150.1 68418.m02526 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}; contains Pfam
profile: PF02170 PAZ (Piwi Argonaut and Zwille), PF02171
Piwi domain
Length = 896
Score = 40.3 bits (90), Expect = 0.001
Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +3
Query: 282 GLEIANEFYQVEAKILEAPTLEVGPRQFTVPKKGVW--QANCLLKPEALNSWGFIAIELD 455
G+ I F QVE +IL P L+VG + P KG W L +P + W +
Sbjct: 430 GVSIITNFTQVEGRILPTPMLKVGKGENLSPIKGKWNFMRKTLAEPTTVTRWA--VVNFS 487
Query: 456 PRGCNYEDIVSKLMNTGRK 512
R C+ ++ L+ GR+
Sbjct: 488 AR-CDTNTLIRDLIKCGRE 505
Score = 27.9 bits (59), Expect = 7.0
Identities = 15/52 (28%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +2
Query: 554 IRINDLHKSMLHALEKQVNFLVVVVSGR-GRDYYHKLKQIAELKVGILTQCI 706
+R+ ++ + + L K FL+ +++ R D Y K+ + +GI+TQCI
Sbjct: 531 VRVENMFEQIKSKLPKPPLFLLCILAERKNSDVYGPWKKKNLVDLGIVTQCI 582
>At5g21030.1 68418.m02501 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}, SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 850
Score = 40.3 bits (90), Expect = 0.001
Identities = 23/76 (30%), Positives = 35/76 (46%)
Frame = +3
Query: 282 GLEIANEFYQVEAKILEAPTLEVGPRQFTVPKKGVWQANCLLKPEALNSWGFIAIELDPR 461
G+ I ++F QVE ++L P L+ G Q P G W N KP + W + R
Sbjct: 411 GVRIGSDFTQVEGRVLPTPKLKAGKEQDIYPINGSW--NFKNKPATVTRWA--VVNFSAR 466
Query: 462 GCNYEDIVSKLMNTGR 509
C+ + I+ L G+
Sbjct: 467 -CDPQKIIDDLTRCGK 481
>At1g31280.1 68414.m03828 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}, SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 1013
Score = 40.3 bits (90), Expect = 0.001
Identities = 21/84 (25%), Positives = 44/84 (52%), Gaps = 1/84 (1%)
Frame = +1
Query: 4 RMTVYEYFMKEKKYRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTM 183
+ ++ EYF + I + D+ CL +G + ++PME ++ GQ K D+ +
Sbjct: 438 KTSIVEYFRIKYGRHIVHKDIPCLDLGKNGRQNFVPMEFCDLVEGQIYPKDNLDKDSALW 497
Query: 184 VRE-AATPPDVRKRKIEEVIQKMN 252
+++ + P R+R I+++I+ N
Sbjct: 498 LKKLSLVNPQQRQRNIDKMIKARN 521
Score = 31.9 bits (69), Expect = 0.43
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +2
Query: 614 LVVVVSGRGRDYYHKLKQIAELKVGILTQC 703
LV+ R D Y LK IAE K+G++TQC
Sbjct: 665 LVLCAMSRKDDGYKTLKWIAETKLGLVTQC 694
>At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi
domain-containing protein contains Pfam profiles
PF02170: PAZ domain, PF02171: Piwi domain
Length = 1194
Score = 38.7 bits (86), Expect = 0.004
Identities = 21/81 (25%), Positives = 42/81 (51%), Gaps = 1/81 (1%)
Frame = +1
Query: 4 RMTVYEYFMKEKKYRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTM 183
++++ EYF ++ I + D+ CL +G K + ++PME + GQ K+ R +
Sbjct: 613 KISIVEYFKEKYGRDIDHKDIPCLNLGKKGRENFVPMEFCNLVEGQIFPKEKLYRDSAAW 672
Query: 184 VRE-AATPPDVRKRKIEEVIQ 243
++E + P R I ++I+
Sbjct: 673 LKELSLVTPQQRLENINKMIK 693
Score = 29.1 bits (62), Expect = 3.0
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 614 LVVVVSGRGRDYYHKLKQIAELKVGILTQC 703
LV+ D Y LK IAE K+G++TQC
Sbjct: 841 LVLCAMTGKHDGYKTLKWIAETKLGLVTQC 870
>At1g31730.1 68414.m03893 epsilon-adaptin, putative similar to
SP|Q9UPM8 Adapter-related protein complex 4 epsilon 1
subunit (Epsilon subunit of AP-4) (AP-4 adapter complex
epsilon subunit) {Homo sapiens}; contains Pfam profile:
PF01602 Adaptin N terminal region
Length = 938
Score = 28.7 bits (61), Expect = 4.0
Identities = 21/61 (34%), Positives = 35/61 (57%), Gaps = 3/61 (4%)
Frame = +1
Query: 112 MELVEVAYGQARNKQLNDRQLSTMV---REAATPPDVRKRKIEEVIQKMNYRRINSLKHT 282
++LV+ + G+AR+K DR + + V + PD+ KRK++E I ++ Y I L H
Sbjct: 26 LDLVK-SIGEARSKAEEDRIVLSEVDILKRRLLEPDIPKRKMKEYIIRLVY--IEMLGHD 82
Query: 283 A 285
A
Sbjct: 83 A 83
>At5g60740.1 68418.m07621 ABC transporter family protein similar to
ATP-binding cassette, sub-family G, member 2
(Placenta-specific ATP- binding cassette transporter)
(Breast cancer resistance protein) SP:Q9UNQ0 from [Homo
sapiens]
Length = 1061
Score = 27.9 bits (59), Expect = 7.0
Identities = 19/72 (26%), Positives = 31/72 (43%), Gaps = 4/72 (5%)
Frame = +1
Query: 43 YRIKYPDLNCLWVGPKDKNIYLPMELVEVAYGQARNKQLNDRQLSTM----VREAATPPD 210
+ ++ D N PK K ++ ++ AYGQ ++ Q + V A D
Sbjct: 431 FNLEIGDKNIKKHAPKGKALHTQSQMFRYAYGQIEKEKAMQEQNKNLTFSGVISMANDID 490
Query: 211 VRKRKIEEVIQK 246
+RKR + EV K
Sbjct: 491 IRKRPMIEVAFK 502
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,050,497
Number of Sequences: 28952
Number of extensions: 383384
Number of successful extensions: 871
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 839
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 871
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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