Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021241
(836 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ... 63 4e-11
SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pom... 58 2e-09
SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity ... 43 5e-05
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 42 1e-04
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 33 0.038
SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 31 0.27
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 30 0.35
SPBC776.07 |||mitochondrial Mam33 family protein|Schizosaccharom... 29 0.62
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 28 1.4
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 28 1.4
SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 27 2.5
SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces pomb... 27 2.5
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 27 3.3
SPBC30B4.07c |tfb4||transcription factor TFIIH complex subunit T... 27 3.3
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 27 4.4
SPAC4D7.08c |ade4|min13, aza1|amidophosphoribosyltransferase |Sc... 27 4.4
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi... 27 4.4
SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe... 26 7.6
SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pomb... 26 7.6
>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 63.3 bits (147), Expect = 4e-11
Identities = 31/76 (40%), Positives = 46/76 (60%), Gaps = 2/76 (2%)
Frame = +2
Query: 260 NTRVFNLGEYRR-HATSAYTSHEFFRADNKEAMAIRQQCALDALQDVCEWL-TKGGEVAV 433
N + FN+G +RR HA+ ++ FF N+EA +R+ A+D L + +W + G V +
Sbjct: 26 NCKAFNVGNFRRKHASHSHDDASFFDPSNEEASKLRESFAMDTLDALLQWFEEEDGVVGI 85
Query: 434 FDATNSTLDRRRMIRD 481
FDATNST RR+ I D
Sbjct: 86 FDATNSTSKRRKAIVD 101
Score = 56.8 bits (131), Expect = 4e-09
Identities = 32/98 (32%), Positives = 54/98 (55%), Gaps = 1/98 (1%)
Frame = +1
Query: 514 FVESICDDPRIIEQNIMEVKVSSPDYTNIQNTDNVLNDFMLRIEHYQEKYEPLDESLES- 690
F+ESIC+D ++I N M +K+ PDY ++ N + L DF R+ Y+ KYEPL +S E
Sbjct: 113 FIESICNDEQLIAAN-MRMKLVGPDYKDL-NPEQSLRDFQERVRMYERKYEPLGKSEEDL 170
Query: 691 EYSFMEDIRYRREGGRSQARRAHTSRIVYYLMTYILYP 804
+++ I ++ R + V++L+ + L P
Sbjct: 171 NLRYIKVINVGKKVVAFNIRGFLAGQAVFFLLNFNLSP 208
Score = 42.3 bits (95), Expect = 8e-05
Identities = 17/24 (70%), Positives = 20/24 (83%)
Frame = +3
Query: 186 MVGLPARGKTYISKKLSRYLNWIE 257
MVGLPARGK+YI KL RY NW++
Sbjct: 1 MVGLPARGKSYIVNKLVRYYNWLQ 24
>SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 592
Score = 57.6 bits (133), Expect = 2e-09
Identities = 29/81 (35%), Positives = 48/81 (59%), Gaps = 3/81 (3%)
Frame = +2
Query: 254 RINTRVFNLGEYRRH-ATSAYT-SHEFFRADNKEAMAIRQQCALDALQDVCEWLT-KGGE 424
+ N R F++G+ RR S Y+ S +FF N+E R+ AL+ L+D+ W+ + G
Sbjct: 171 KYNCRFFSVGDKRREEGASTYSMSADFFDIKNEETFKFRENVALETLEDLLHWMIHENGV 230
Query: 425 VAVFDATNSTLDRRRMIRDLV 487
+ + DATNST +RR+ + D +
Sbjct: 231 IGILDATNSTHERRKHLYDRI 251
Score = 53.6 bits (123), Expect = 3e-08
Identities = 25/77 (32%), Positives = 48/77 (62%)
Frame = +1
Query: 487 SPQDGLQVFFVESICDDPRIIEQNIMEVKVSSPDYTNIQNTDNVLNDFMLRIEHYQEKYE 666
S + + + F+ES+C D + E+NI ++K+ PDY +T++ L D R++ Y++ YE
Sbjct: 252 SKEADIGIMFLESLCTDDILFEENI-KLKIKGPDYEGY-DTESALKDLRERVDLYKKYYE 309
Query: 667 PLDESLESEYSFMEDIR 717
PLDE + + F++ ++
Sbjct: 310 PLDER-DEQLPFLQYVK 325
Score = 44.4 bits (100), Expect = 2e-05
Identities = 19/47 (40%), Positives = 31/47 (65%), Gaps = 1/47 (2%)
Frame = +3
Query: 120 LNKQFFGDGERSNY-VNIPHVIAMVGLPARGKTYISKKLSRYLNWIE 257
++K+ D ++Y + +I +VG+PA GK+YI KLSRY NW++
Sbjct: 125 VSKRTENDSNNASYGIREKLIIILVGIPATGKSYIGSKLSRYYNWLK 171
>SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 408
Score = 43.2 bits (97), Expect = 5e-05
Identities = 27/92 (29%), Positives = 48/92 (52%)
Frame = +1
Query: 508 VFFVESICDDPRIIEQNIMEVKVSSPDYTNIQNTDNVLNDFMLRIEHYQEKYEPLDESLE 687
+ FVE +C + +II++NI ++ SP + + ++ N + I Y++ Y PL E+
Sbjct: 111 LMFVEVVCTNQKIIDENITDMCQHSPYFKSFPFEES-KNKILDSIHEYEKHYTPLSEA-- 167
Query: 688 SEYSFMEDIRYRREGGRSQARRAHTSRIVYYL 783
E +F+ + + E + SRIVYYL
Sbjct: 168 EECTFVRIVDFGAELIVHKLENYLESRIVYYL 199
Score = 36.7 bits (81), Expect = 0.004
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = +3
Query: 180 IAMVGLPARGKTYISKKLSRYLNWIESIQEYL 275
+ VGLPA GKT + KLSRYL W+ S+ +L
Sbjct: 12 VCFVGLPASGKTSSAMKLSRYLTWM-SVSTHL 42
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 41.9 bits (94), Expect = 1e-04
Identities = 27/101 (26%), Positives = 52/101 (51%), Gaps = 1/101 (0%)
Frame = +1
Query: 499 GLQVFFVESICDDPRIIEQNIMEVKVSSPDYTNIQNTDNVLNDFMLRIEHYQEKYEPLDE 678
G ++ F+ES+C+ +I NI E S ++ N + + ++ RI+ + YE +D
Sbjct: 162 GFKILFIESLCNKEDVINANIQEAIHVSEEFRN-WDLEMAEKEYCRRIDILKCHYETID- 219
Query: 679 SLESEYSFMEDIRYRREGGRSQARRAH-TSRIVYYLMTYIL 798
E +YSF++ I + +++ + SRI++ LM L
Sbjct: 220 --EKDYSFVKMINFAETIIANKSNEGYLLSRILFLLMNMTL 258
Score = 37.5 bits (83), Expect = 0.002
Identities = 31/116 (26%), Positives = 52/116 (44%), Gaps = 1/116 (0%)
Frame = +2
Query: 260 NTRVFNLGEYRRHATSAYTSHEFFRADNKEAMAIRQQCALDALQDVCEWLTKG-GEVAVF 436
N R ++L +Y T TS A ++ + R L D+ +L K G+VA++
Sbjct: 82 NCRFYSLAKYIDERTREMTSSPVKSAASENHVFSRNDTIERCLADLEIFLLKEKGQVAIY 141
Query: 437 DATNSTLDRRRMIRDLVVHKMGFKFSLLNLYVMTLGLLNKTLWK*K*AVRTTQTFR 604
DATN T RR++ D GFK + ++N + + A+ ++ FR
Sbjct: 142 DATNGTRRTRRILYDR-FKNCGFKILFIESLCNKEDVINANIQE---AIHVSEEFR 193
Score = 36.3 bits (80), Expect = 0.005
Identities = 15/26 (57%), Positives = 19/26 (73%)
Frame = +3
Query: 177 VIAMVGLPARGKTYISKKLSRYLNWI 254
VI M GLPARGK+ I+ + RYL W+
Sbjct: 54 VIIMSGLPARGKSNIAVSIDRYLRWL 79
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 33.5 bits (73), Expect = 0.038
Identities = 18/43 (41%), Positives = 24/43 (55%)
Frame = +2
Query: 365 QQCALDALQDVCEWLTKGGEVAVFDATNSTLDRRRMIRDLVVH 493
+Q LDA++DV EWL GE DA+ + +R D VVH
Sbjct: 596 KQAVLDAVEDVAEWLEIHGE----DASKEEFEDQRQKLDAVVH 634
>SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 815
Score = 30.7 bits (66), Expect = 0.27
Identities = 21/72 (29%), Positives = 35/72 (48%), Gaps = 6/72 (8%)
Frame = -3
Query: 591 VVRTAHFYFHNVLFNNPRVITYRFNKENLKPILWTTRSLII------RRRSKVEFVASKT 430
+VR H F+ VL+ N R + + N + +W+ S + R + ++ +ASK
Sbjct: 561 IVREIHHAFYKVLYANARFPSMSVSMANWESDVWSLASSTLIDFVQHRNQHDMKKLASKM 620
Query: 429 ATSPPLVSHSHT 394
TS P+ S S T
Sbjct: 621 TTSLPVFSTSIT 632
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1811
Score = 30.3 bits (65), Expect = 0.35
Identities = 18/76 (23%), Positives = 35/76 (46%)
Frame = +2
Query: 287 YRRHATSAYTSHEFFRADNKEAMAIRQQCALDALQDVCEWLTKGGEVAVFDATNSTLDRR 466
+R H S + S F++ +I +C L L C W E+ D + + +R
Sbjct: 1591 FRPHEISRFNSQSVFKSKKHHLKSIVVKCTLQLLMLNCLW-----ELFHSDNMLTNIPKR 1645
Query: 467 RMIRDLVVHKMGFKFS 514
+M++ L + K ++F+
Sbjct: 1646 KMVKLLDILKQSWEFA 1661
>SPBC776.07 |||mitochondrial Mam33 family
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 29.5 bits (63), Expect = 0.62
Identities = 8/19 (42%), Positives = 17/19 (89%)
Frame = +3
Query: 219 ISKKLSRYLNWIESIQEYL 275
++K+L Y+NW+ES++++L
Sbjct: 250 LTKELKEYINWLESVRQFL 268
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 28.3 bits (60), Expect = 1.4
Identities = 26/87 (29%), Positives = 37/87 (42%), Gaps = 1/87 (1%)
Frame = +1
Query: 526 ICDDPRIIEQNIMEVKVSSPDYTNIQNTDNVLNDFMLRI-EHYQEKYEPLDESLESEYSF 702
+ D P ++ N + S DYT + N N + LR+ E E L +L S S
Sbjct: 689 VFDRPLLLLLNSLLEDESGRDYTAMMNWINASSSLQLRVLELMTENLLSLSPNLTSVNS- 747
Query: 703 MEDIRYRREGGRSQARRAHTSRIVYYL 783
+E R Q R + SR VY+L
Sbjct: 748 LETAMTRYNINEDQ-RNSEASRCVYFL 773
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 28.3 bits (60), Expect = 1.4
Identities = 15/49 (30%), Positives = 28/49 (57%), Gaps = 1/49 (2%)
Frame = +3
Query: 228 KLSRYLNWIESIQEYLI*ANTEDMLHPHTQAMSSSGP-TTKKPWL*DSS 371
KLS Y+NW +++ ++I + + L+P + + P TK P L D++
Sbjct: 1296 KLSEYVNWRIALERFVINESHDSALYPLLHFVLDNLPANTKAPELDDTN 1344
>SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 27.5 bits (58), Expect = 2.5
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -1
Query: 764 LVCALRACERPPSLRYRISSMKLYSDSRLSS 672
+VC LRA PP +R R + K++S ++ S
Sbjct: 447 IVCTLRAAYDPPKVRTRSTLGKVFSTNKRKS 477
>SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 827
Score = 27.5 bits (58), Expect = 2.5
Identities = 13/36 (36%), Positives = 22/36 (61%), Gaps = 2/36 (5%)
Frame = +1
Query: 643 EHYQEKYEPLDESLESEYSFMEDIR--YRREGGRSQ 744
E Y+++ E LDE +E SF+ D + R+ G ++Q
Sbjct: 228 ERYEDEREKLDEQMEDLQSFLSDYKKASRKSGIKTQ 263
>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 27.1 bits (57), Expect = 3.3
Identities = 10/37 (27%), Positives = 22/37 (59%)
Frame = -3
Query: 537 VITYRFNKENLKPILWTTRSLIIRRRSKVEFVASKTA 427
+++YR + N++P++W I+ S++EF+ A
Sbjct: 264 LMSYRMSS-NVRPLIWVECESIVHSGSRIEFMVKAKA 299
>SPBC30B4.07c |tfb4||transcription factor TFIIH complex subunit Tfb4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 27.1 bits (57), Expect = 3.3
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
Frame = -3
Query: 267 LVLILSSSGSVTVSWKCMSSRVPGDPPLR*HAVCLHNLIVLHH-RRTAYLNTSSLQLGFR 91
LV+IL ++ + SW +S +VP L V L+ + HH R A L + S ++ +
Sbjct: 26 LVVILDANPA---SWYSLSKKVPVSKVLADITVFLNAHLAFHHDNRVAVLASHSDKVEYL 82
Query: 90 HPS 82
+PS
Sbjct: 83 YPS 85
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 26.6 bits (56), Expect = 4.4
Identities = 18/74 (24%), Positives = 30/74 (40%)
Frame = +3
Query: 111 WKYLNKQFFGDGERSNYVNIPHVIAMVGLPARGKTYISKKLSRYLNWIESIQEYLI*ANT 290
WK +FG E+ N+P + V L TY+ + L I ++Q L A +
Sbjct: 706 WK--QPAYFGKLEQEQKTNLPFFVKFVALMLNDATYLLDEALLKLTEIHNLQSLLADAIS 763
Query: 291 EDMLHPHTQAMSSS 332
+ + Q S+
Sbjct: 764 NSNSNQNVQESQSN 777
>SPAC4D7.08c |ade4|min13, aza1|amidophosphoribosyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 533
Score = 26.6 bits (56), Expect = 4.4
Identities = 18/80 (22%), Positives = 35/80 (43%)
Frame = +1
Query: 454 FGPTADDKRPCSPQDGLQVFFVESICDDPRIIEQNIMEVKVSSPDYTNIQNTDNVLNDFM 633
+G T D+ DG+ +E + D R E EV + + +YT + + +++
Sbjct: 435 YGKTEDEVAEAISADGVIYQTLEDLLDSCRTAELTEFEVGLFTGEYTTGASKEYLVHLEQ 494
Query: 634 LRIEHYQEKYEPLDESLESE 693
+RI + + + E E E
Sbjct: 495 MRIANNRARKHSFAEDEERE 514
>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
reductase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1053
Score = 26.6 bits (56), Expect = 4.4
Identities = 19/51 (37%), Positives = 27/51 (52%)
Frame = -3
Query: 348 SLLSALKNSWLVYADVACLLYSPRLNTLVLILSSSGSVTVSWKCMSSRVPG 196
SL SAL + LV + + L LNT + S+ T + K ++SRVPG
Sbjct: 1004 SLCSALASGHLVKSHIG--LNRSALNTPAMDSSAKKPATDALKSVNSRVPG 1052
>SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 270
Score = 25.8 bits (54), Expect = 7.6
Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = -3
Query: 363 LIAMASL-LSALKNSWLVYADVACLLY 286
LI + SL + K +W++YAD+ CL Y
Sbjct: 130 LINLQSLCIFEKKAAWVLYADIICLNY 156
>SPBC1685.07c |||amino acid transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 420
Score = 25.8 bits (54), Expect = 7.6
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +1
Query: 40 IANNILSAVLLGIPRWMTKTELKAGSI*ISSSS 138
+AN IL A +L +P TKT L G + I S+
Sbjct: 28 LANTILGAGILSLPNAFTKTGLLFGCLTIVFSA 60
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,726,955
Number of Sequences: 5004
Number of extensions: 81918
Number of successful extensions: 304
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 276
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 300
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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