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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021238
         (743 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosacchar...   118   7e-28
SPBC713.06 |adl1|lig3|DNA ligase |Schizosaccharomyces pombe|chr ...    67   2e-12
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch...    60   3e-10
SPBC1348.05 |||membrane transporter|Schizosaccharomyces pombe|ch...    25   8.6  
SPAC1786.01c ||SPAC31G5.20c|triacylglycerol lipase|Schizosacchar...    25   8.6  
SPBPB2B2.16c |||MFS family membrane transporter |Schizosaccharom...    25   8.6  
SPAC750.02c |||membrane transporter|Schizosaccharomyces pombe|ch...    25   8.6  

>SPAC20G8.01 |cdc17||ATP-dependent DNA ligase
           Cdc17|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 768

 Score =  118 bits (285), Expect = 7e-28
 Identities = 50/76 (65%), Positives = 64/76 (84%)
 Frame = -3

Query: 738 FLEEAVKESCEGLMVKMLTGDNSKYDIARRSHNWLKLKQDYLEGVGDTVDAVIIGAYRGK 559
           FLEE+VK+SCEGLMVKML G +S Y+ ++RS +WLK+K+DYL GVGD++D ++IGAY GK
Sbjct: 558 FLEESVKDSCEGLMVKMLEGPDSHYEPSKRSRHWLKVKKDYLSGVGDSLDLIVIGAYYGK 617

Query: 558 GKRAGVYGGFLVACYD 511
           GKR  VYG FL+ CYD
Sbjct: 618 GKRTSVYGAFLLGCYD 633



 Score = 67.3 bits (157), Expect = 2e-12
 Identities = 33/91 (36%), Positives = 51/91 (56%), Gaps = 2/91 (2%)
 Frame = -2

Query: 511 PESEQFQSLCKLGTGFSDEDLQALSDMLKEHIIDSPRNYYNYDS--SHSADVWTSAAVVV 338
           P++E  QS+CKLGTGFS+E L+   + LK+ +I   +++Y +    +H  DVW     + 
Sbjct: 634 PDTETVQSICKLGTGFSEEHLETFYNQLKDIVISKKKDFYAHSDVPAHQPDVWFEPKYLW 693

Query: 337 EVPAQTCPSRSAHRAAIARLHPHKGVSLASP 245
           EV A        ++AAI  +   KG+SL  P
Sbjct: 694 EVLAADLSLSPVYKAAIGYVQEDKGISLRFP 724


>SPBC713.06 |adl1|lig3|DNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 774

 Score = 67.3 bits (157), Expect = 2e-12
 Identities = 34/80 (42%), Positives = 50/80 (62%), Gaps = 4/80 (5%)
 Frame = -3

Query: 738 FLEEAVKESCEGLMVKMLTGDNSK----YDIARRSHNWLKLKQDYLEGVGDTVDAVIIGA 571
           F   A+   CEGLMVK+L G NSK    Y+  +R   W+K+KQDY +   +++D V IGA
Sbjct: 574 FFSLAITNKCEGLMVKVLNGTNSKFPSTYEPDKRGEGWIKVKQDY-DDEFESLDLVPIGA 632

Query: 570 YRGKGKRAGVYGGFLVACYD 511
           + G G++AG +   L+A Y+
Sbjct: 633 WYGNGRKAGWFSPILLAVYN 652



 Score = 33.9 bits (74), Expect = 0.025
 Identities = 21/92 (22%), Positives = 45/92 (48%), Gaps = 3/92 (3%)
 Frame = -2

Query: 511 PESEQFQSLCKLGTGFSDEDLQALSDMLK-EHIIDSPRNYYNY--DSSHSADVWTSAAVV 341
           P++  ++++CK  +GFSD+  + L+     E    S +  YN+      +  ++ +   V
Sbjct: 653 PDTGAYEAVCKCMSGFSDQFYKELTQKYSLESGNSSLKPIYNFCETGKVTPQIYFAPQEV 712

Query: 340 VEVPAQTCPSRSAHRAAIARLHPHKGVSLASP 245
            E+      S  A++AA+  +   +G+S+  P
Sbjct: 713 WEIKGAQITSSPAYKAALGLIQDDRGLSIRFP 744


>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 923

 Score = 60.1 bits (139), Expect = 3e-10
 Identities = 26/70 (37%), Positives = 43/70 (61%)
 Frame = -3

Query: 735 LEEAVKESCEGLMVKMLTGDNSKYDIARRSHNWLKLKQDYLEGVGDTVDAVIIGAYRGKG 556
           L   ++E  EGL++K  +G    Y +  R  +W+K+K  YL+G G+ +D +I+G Y G+G
Sbjct: 443 LRNVIQEGSEGLVIKKPSGS---YHLGERMDDWIKVKPYYLQGFGEDLDCLILGGYFGRG 499

Query: 555 KRAGVYGGFL 526
           K++G    FL
Sbjct: 500 KQSGKINSFL 509


>SPBC1348.05 |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 485

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 12/22 (54%), Positives = 13/22 (59%)
 Frame = +3

Query: 441 KACRSSSEKPVPSLHSDWNCSD 506
           K C SSS K VPS   + NC D
Sbjct: 8   KKCDSSSIKHVPSTPLETNCPD 29


>SPAC1786.01c ||SPAC31G5.20c|triacylglycerol
           lipase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 630

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 14/49 (28%), Positives = 20/49 (40%)
 Frame = -2

Query: 415 IDSPRNYYNYDSSHSADVWTSAAVVVEVPAQTCPSRSAHRAAIARLHPH 269
           + SP    NY +S    +W++      VP    P     R+   RL PH
Sbjct: 373 VHSPPKLINYLTSPDTVIWSAVIASCAVPGILNPIPLMTRSQSHRLIPH 421


>SPBPB2B2.16c |||MFS family membrane transporter
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 485

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 12/22 (54%), Positives = 13/22 (59%)
 Frame = +3

Query: 441 KACRSSSEKPVPSLHSDWNCSD 506
           K C SSS K VPS   + NC D
Sbjct: 8   KKCDSSSIKHVPSTPLETNCPD 29


>SPAC750.02c |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 485

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 12/22 (54%), Positives = 13/22 (59%)
 Frame = +3

Query: 441 KACRSSSEKPVPSLHSDWNCSD 506
           K C SSS K VPS   + NC D
Sbjct: 8   KKCDSSSIKHVPSTPLETNCPD 29


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,038,610
Number of Sequences: 5004
Number of extensions: 28928
Number of successful extensions: 108
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 353266144
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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