Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021230
(772 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 151 1e-37
SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p... 36 0.006
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 32 0.079
SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces p... 32 0.10
SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 30 0.42
SPAC144.02 |||transcription factor |Schizosaccharomyces pombe|ch... 29 0.56
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 28 1.3
SPBC646.13 |sds23|psp1, moc1|inducer of sexual development Sds23... 28 1.3
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 27 2.2
SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|c... 27 2.2
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 27 3.0
SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr ... 27 3.0
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 27 3.0
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 27 3.9
SPAC9.12c |atp12||F1-ATPase chaperone Atp12 |Schizosaccharomyces... 27 3.9
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 26 5.2
SPBC725.16 |res1|sct1|MBF transcription factor complex subunit R... 26 6.9
SPAC4G9.05 |mpf1||meiotic PUF family protein 1|Schizosaccharomyc... 26 6.9
SPAC15A10.02 |taf12||transcription factor TFIID complex subunit ... 26 6.9
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 25 9.1
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 25 9.1
SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosacc... 25 9.1
>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 503
Score = 151 bits (366), Expect = 1e-37
Identities = 79/112 (70%), Positives = 85/112 (75%), Gaps = 1/112 (0%)
Frame = +1
Query: 427 PNL-GRRLLGHPGSAHQPMVTYLPRGDD*TGISAIDVMNSIARGQKIPIFSAAGLPHNEI 603
PNL L GS P P TGIS+ID +NSIARGQKIPIFSAAGLPHNEI
Sbjct: 120 PNLLAEDYLDINGSPINPYARIYPEEMIQTGISSIDGLNSIARGQKIPIFSAAGLPHNEI 179
Query: 604 AAQICRQAGLVKVPGKSVLDDHEDNFAIVFAAMGVNMETARFFKQDFEENGS 759
AAQICRQAGLVK P K V D HEDNF+IVFAAMGVN+ETARFF++DFEENGS
Sbjct: 180 AAQICRQAGLVKRPTKDVHDGHEDNFSIVFAAMGVNLETARFFQRDFEENGS 231
Score = 140 bits (339), Expect = 2e-34
Identities = 63/86 (73%), Positives = 74/86 (86%)
Frame = +2
Query: 254 VLEVSGSKAVVQVFEGTSGIDAKNTLCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPPI 433
VLEV+G KA+VQVFEGTSG+D + T +FTG +R PVSEDMLGRVFNGSG PIDKGP +
Sbjct: 63 VLEVAGHKAIVQVFEGTSGVDVRKTTIDFTGHSMRIPVSEDMLGRVFNGSGLPIDKGPNL 122
Query: 434 LAEDFLDIQGQPINPWSRIYPEEMIK 511
LAED+LDI G PINP++RIYPEEMI+
Sbjct: 123 LAEDYLDINGSPINPYARIYPEEMIQ 148
Score = 64.5 bits (150), Expect = 2e-11
Identities = 27/52 (51%), Positives = 40/52 (76%)
Frame = +3
Query: 99 AVSRDFISQPRLTYKTVSGVNGPLVILDEVKFPKFSEIVQLKLADGTLRSGQ 254
A +D+ +PRL+Y TV+ + GPLVILD ++ P+++EIV L L DG++RSGQ
Sbjct: 11 AAVKDYSIKPRLSYNTVNSITGPLVILDNIRRPQYNEIVNLNLPDGSVRSGQ 62
>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 525
Score = 35.9 bits (79), Expect = 0.006
Identities = 25/72 (34%), Positives = 36/72 (50%), Gaps = 4/72 (5%)
Frame = +2
Query: 254 VLEVS---GSKAVVQV-FEGTSGIDAKNTLCEFTGDILRTPVSEDMLGRVFNGSGKPIDK 421
VLEV+ G V + +GT G+ + T TG + PV LGR+ N G+P+D+
Sbjct: 93 VLEVAQHVGENTVRTIAMDGTEGL-VRGTAVIDTGSPISIPVGPGTLGRIMNVIGEPVDE 151
Query: 422 GPPILAEDFLDI 457
PI A + I
Sbjct: 152 RGPIKAVKYSPI 163
Score = 29.1 bits (62), Expect = 0.74
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 511 TGISAIDVMNSIARGQKIPIFSAAGL 588
TGI +D++ ARG KI +F AG+
Sbjct: 182 TGIKVVDLLAPYARGGKIGLFGGAGV 207
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 32.3 bits (70), Expect = 0.079
Identities = 19/66 (28%), Positives = 35/66 (53%)
Frame = -2
Query: 396 LNTRPNMSSETGVRRMSPVNSQSVFFASIPDVPSNTWTTALEPLTSSTDQNEGFHLQALA 217
LN+ ++S + + +P+ S SV +S P + S+T + P+TSST N + + +
Sbjct: 1230 LNSSTPITSSSILNSSTPITSSSVLNSSTP-ITSSTVVNSSTPITSSTALNTSIPITSSS 1288
Query: 216 VQSQRT 199
V + T
Sbjct: 1289 VLNSST 1294
Score = 31.5 bits (68), Expect = 0.14
Identities = 18/66 (27%), Positives = 35/66 (53%)
Frame = -2
Query: 396 LNTRPNMSSETGVRRMSPVNSQSVFFASIPDVPSNTWTTALEPLTSSTDQNEGFHLQALA 217
LN+ ++S + + +P+ S S+ +S P + S+T + P+TSST N + + +
Sbjct: 3114 LNSSTAITSSSILNSSTPITSSSILNSSTP-ITSSTVVNSSTPITSSTTLNTSTPITSSS 3172
Query: 216 VQSQRT 199
V + T
Sbjct: 3173 VLNSST 3178
Score = 29.5 bits (63), Expect = 0.56
Identities = 18/66 (27%), Positives = 32/66 (48%)
Frame = -2
Query: 396 LNTRPNMSSETGVRRMSPVNSQSVFFASIPDVPSNTWTTALEPLTSSTDQNEGFHLQALA 217
LN+ ++S T + +P+ S + S P + S+T + P+TSST N + +
Sbjct: 1626 LNSSTPITSSTVLNSSTPITSSTALNTS-PPITSSTVVNSSTPITSSTVVNTSTPITSST 1684
Query: 216 VQSQRT 199
V + T
Sbjct: 1685 VVNSST 1690
>SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 536
Score = 31.9 bits (69), Expect = 0.10
Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 290 VFEGTSGIDAKNTLCEFTGDILRTPVSEDMLGRVFNGSGKPID-KGP 427
V G + + + + T I+ PV E +LGRV + G PID KGP
Sbjct: 102 VLFGNDRLVREGEVVKRTRHIVDVPVGEALLGRVVDALGNPIDGKGP 148
Score = 26.6 bits (56), Expect = 3.9
Identities = 23/85 (27%), Positives = 32/85 (37%)
Frame = +1
Query: 511 TGISAIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKVPGKSVLDDHEDNFAIV 690
TG+ AID M I RGQ+ I IA ++ + D V
Sbjct: 176 TGLKAIDSMVPIGRGQRELIIGDRQTGKTAIALDT-----ILNHKRWNNSSDESKKLYCV 230
Query: 691 FAAMGVNMETARFFKQDFEENGSMR 765
+ A+G T Q EEN S++
Sbjct: 231 YVAVGQKRSTVAQLVQKLEENDSLK 255
>SPAC11E3.11c |||guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 942
Score = 29.9 bits (64), Expect = 0.42
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +2
Query: 647 ASQSWTTTRTTSPLCSPLWVSTWKRPDSSSR 739
+ +S T+TR++ P L+ STW + +SSSR
Sbjct: 28 SQRSKTSTRSSKPFLQRLFPSTWFKNESSSR 58
>SPAC144.02 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 29.5 bits (63), Expect = 0.56
Identities = 17/32 (53%), Positives = 20/32 (62%), Gaps = 3/32 (9%)
Frame = -2
Query: 564 DLLTTGNRVHHINSGDTS---LIISSG*IRDH 478
DLLT N VHHI++G TS LI + I DH
Sbjct: 34 DLLTLDNLVHHIHNGTTSNLRLISNINSILDH 65
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 28.3 bits (60), Expect = 1.3
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = -2
Query: 624 LSTDLGGNFIVRQTGGREDGDLLTTGNRVHHINSGDTSLIISSG 493
LST + FI R + ++ L +GN HH + D + + G
Sbjct: 312 LSTVINEPFISRSSSLTDERGLADSGNSYHHFSDSDDESLFNDG 355
>SPBC646.13 |sds23|psp1, moc1|inducer of sexual development
Sds23/Moc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 408
Score = 28.3 bits (60), Expect = 1.3
Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 3/41 (7%)
Frame = -2
Query: 399 PLNTRPNMSSETGVRRMSPVNSQSVFFASIP---DVPSNTW 286
PL+T+ + S + R+ VNS S F A P ++PSN W
Sbjct: 2 PLSTQSSDSLSSAGRQSFGVNSVSDFLAQFPIPTNLPSNKW 42
>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 619
Score = 27.5 bits (58), Expect = 2.2
Identities = 13/54 (24%), Positives = 28/54 (51%)
Frame = +2
Query: 254 VLEVSGSKAVVQVFEGTSGIDAKNTLCEFTGDILRTPVSEDMLGRVFNGSGKPI 415
V+ + K +QV+E TSG+ + + + TG L + + +++G +P+
Sbjct: 61 VIRIHQDKCTIQVYEETSGLTVGDPV-QRTGKPLSVELGPGLAETIYDGIQRPL 113
>SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|chr
3|||Manual
Length = 594
Score = 27.5 bits (58), Expect = 2.2
Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 2/70 (2%)
Frame = -2
Query: 462 PWMSKKSSAKIGGPLSMGLPEPLNTRPNMSSETGVRRMSPVNSQSV--FFASIPDVPSNT 289
P +S+++S+ G P S P+N N +G + SP ++ + FF S P S+
Sbjct: 378 PNLSRRNSSDFG-PNS-----PVNIHSNPVPISGQQPSSPASNSNANFFFGSSPQSMSSE 431
Query: 288 WTTALEPLTS 259
T + P+TS
Sbjct: 432 QTDMMSPITS 441
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 27.1 bits (57), Expect = 3.0
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -2
Query: 285 TTALEPLTSSTDQNEGFHLQ 226
TT + LT+ST QN+ HLQ
Sbjct: 591 TTIVNALTNSTSQNKSMHLQ 610
>SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 384
Score = 27.1 bits (57), Expect = 3.0
Identities = 32/89 (35%), Positives = 40/89 (44%), Gaps = 6/89 (6%)
Frame = +2
Query: 269 GSKAVVQV-FEGTSGIDAKNTLCE--FT-GDILRTPVSEDMLGRVFNGSGKPIDKG-PPI 433
G AVV + G S +D E FT GDI+ LG N GK I K P
Sbjct: 31 GPTAVVMMNMGGPSNLDEVGPFLERLFTDGDIIP-------LGYFQNSLGKFIAKRRTPK 83
Query: 434 LAEDFLDIQG-QPINPWSRIYPEEMIKLV 517
+ + DI G PI W+RI EM K++
Sbjct: 84 VQNHYSDIGGGSPILHWTRIQGSEMCKIL 112
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 27.1 bits (57), Expect = 3.0
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1
Query: 463 TLDVQEVFGQDWGTLVDGFAGTVE 392
T ++ +FG+DW DGF VE
Sbjct: 652 TKNINIIFGEDWNNFPDGFELKVE 675
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 26.6 bits (56), Expect = 3.9
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -2
Query: 330 SVFFASIPDVPSNTWTTALEPLTSSTDQNEG 238
+ FF+S+PD PS+ EP+TS+ N G
Sbjct: 299 NAFFSSMPDRPSSN-----EPMTSNLTSNSG 324
>SPAC9.12c |atp12||F1-ATPase chaperone Atp12 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 287
Score = 26.6 bits (56), Expect = 3.9
Identities = 14/44 (31%), Positives = 23/44 (52%)
Frame = +3
Query: 366 SLKTCWAVYSTVPANPSTRVPQSWPKTSWTSRVSPSTHGHVSTQ 497
S KTC++ YST ++P +PQ + W + + +G V Q
Sbjct: 17 SFKTCYSFYSTKASSP---LPQPSFRRFWKNTATKIQNGEVLIQ 57
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 26.2 bits (55), Expect = 5.2
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = -2
Query: 528 NSGDTSLIISSG*IRDHGLMG*PWMSKKSSAKIGGPLSM 412
NS T + I ++G PW+++KSS G P +M
Sbjct: 246 NSQQTIYVWLDALINYISVIGYPWLNEKSSLSAGWPANM 284
>SPBC725.16 |res1|sct1|MBF transcription factor complex subunit
Res1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 637
Score = 25.8 bits (54), Expect = 6.9
Identities = 15/54 (27%), Positives = 27/54 (50%)
Frame = -2
Query: 417 SMGLPEPLNTRPNMSSETGVRRMSPVNSQSVFFASIPDVPSNTWTTALEPLTSS 256
S L P + NM+S + + + S F PD+PS+++T +L + +S
Sbjct: 140 SHSLLYPYTSSNNMTSTSRMSGIHDALSLQSDFTRSPDMPSDSFTGSLHDIKAS 193
>SPAC4G9.05 |mpf1||meiotic PUF family protein 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 25.8 bits (54), Expect = 6.9
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = -1
Query: 418 VDGFAGTVEYTAQHVFRDRCTKNVTRELAECV 323
+D F V + ++ +R T N+ EL EC+
Sbjct: 337 IDAFGSHVLQKSLEIYPERFTNNLIEELIECL 368
>SPAC15A10.02 |taf12||transcription factor TFIID complex subunit A
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 25.8 bits (54), Expect = 6.9
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = -2
Query: 426 GPLSMGLPEPLNTRP 382
G L G PEP NTRP
Sbjct: 93 GALQQGTPEPSNTRP 107
>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1944
Score = 25.4 bits (53), Expect = 9.1
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +2
Query: 587 CLTMKLPPRSVDRPVLSRF 643
CL KLPP+ VD P S F
Sbjct: 524 CLIDKLPPKKVDIPFKSDF 542
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 25.4 bits (53), Expect = 9.1
Identities = 18/56 (32%), Positives = 25/56 (44%)
Frame = +3
Query: 81 TKEHVLAVSRDFISQPRLTYKTVSGVNGPLVILDEVKFPKFSEIVQLKLADGTLRS 248
TKE + F SQ L+ K + + V+ + +KF QL DG LRS
Sbjct: 422 TKETLTTKVSAFGSQCTLSDKFLKAIKKSSVVEEVLKFATAKADQQLSKGDGGLRS 477
>SPAC12B10.03 |||WD repeat protein, human WDR20
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 543
Score = 25.4 bits (53), Expect = 9.1
Identities = 11/37 (29%), Positives = 17/37 (45%)
Frame = +2
Query: 401 SGKPIDKGPPILAEDFLDIQGQPINPWSRIYPEEMIK 511
+G P+D P+ F+ I + W YP E I+
Sbjct: 41 TGSPLDPNTPVKRLFFMRINIAQFSRWDYPYPREYIE 77
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,500,076
Number of Sequences: 5004
Number of extensions: 77250
Number of successful extensions: 277
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 256
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 276
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -