BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021227 (657 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 69 8e-13 SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 27 3.1 SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.2 SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4 |Schi... 26 5.5 SPAC13A11.05 |||peptidase family M17|Schizosaccharomyces pombe|c... 26 5.5 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 9.6 SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 25 9.6 >SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1207 Score = 68.5 bits (160), Expect = 8e-13 Identities = 30/89 (33%), Positives = 50/89 (56%) Frame = -2 Query: 524 TETYLQMAAYFTHCKLQPVHQILTLRTALNMFFKLKNYRTAASFARRLLELGPRPEVAQQ 345 T+ L+++ YF LQP+H I+ LR A+N KLKNY++A+ +LL+L A+ Sbjct: 1068 TKRALELSYYFASADLQPMHSIIALRLAINASHKLKNYKSASFLGNKLLQLAESGPAAEA 1127 Query: 344 ARKILQACEKTPTDEHQLLYDEHNPFSVC 258 A + + ++ P D ++ YD H +C Sbjct: 1128 ANRAITLGDRNPHDAFEIEYDPHVEMRIC 1156 Score = 37.1 bits (82), Expect = 0.002 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = -1 Query: 243 PIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGLR 115 P+Y G + CS+C A + + ++C VC V IG+ G R Sbjct: 1162 PVYSGDDFDVCSVCGAVYHKGYVNEVCTVCDVGGIGQKGTGRR 1204 >SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 26.6 bits (56), Expect = 3.1 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = -2 Query: 344 ARKILQACEKTPTDEHQLLYDEHNPFSVC 258 +R +L C++ D +Q+L E +P S+C Sbjct: 369 SRGVLSFCDQAIRDLYQILEVEFHPLSIC 397 >SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 224 Score = 26.2 bits (55), Expect = 4.2 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = -1 Query: 633 RPSCPRPNSYWPSAGTTSWDSPWRPP 556 RP+ RPNS+ P +T + SP PP Sbjct: 76 RPTSQRPNSWQPGNASTMYASP--PP 99 >SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 377 Score = 25.8 bits (54), Expect = 5.5 Identities = 23/66 (34%), Positives = 35/66 (53%), Gaps = 4/66 (6%) Frame = -2 Query: 461 ILTLRTALNMFFKLKNYRTAASF----ARRLLELGPRPEVAQQARKILQACEKTPTDEHQ 294 ILT+RT++ + FK+ N + AR L E G E A+ ++LQ K P +E Sbjct: 155 ILTVRTSIPLAFKVSNDQLLMELQLCNARALDETGQFLEAAKCYYRVLQ--YKVPGNE-- 210 Query: 293 LLYDEH 276 L+Y E+ Sbjct: 211 LIYREN 216 >SPAC13A11.05 |||peptidase family M17|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 25.8 bits (54), Expect = 5.5 Identities = 13/26 (50%), Positives = 15/26 (57%) Frame = +1 Query: 64 DTRF*RASLSVEL*GTDTEAERVLAD 141 D F R LSVE+ TD E +LAD Sbjct: 346 DVIFMRNGLSVEIDNTDAEGRLILAD 371 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.0 bits (52), Expect = 9.6 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = -2 Query: 575 TRHGDRQESDAEEHRRRTETYLQMAAYFTHCKLQPVHQILT 453 ++HGD +E D+E+ R ++ +AA + P H + T Sbjct: 1650 SQHGDEEEEDSEDDIRSSKDAAALAAKL-FGGMAPAHPVST 1689 >SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1842 Score = 25.0 bits (52), Expect = 9.6 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -2 Query: 392 ARRLLELGPRPEVAQQARKILQ 327 A R++E+GP P +A A++ L+ Sbjct: 42 AERIVEIGPSPTLAGMAKRTLK 63 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,962,038 Number of Sequences: 5004 Number of extensions: 33828 Number of successful extensions: 132 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 126 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 131 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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