BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021227
(657 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 69 8e-13
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 27 3.1
SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.2
SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4 |Schi... 26 5.5
SPAC13A11.05 |||peptidase family M17|Schizosaccharomyces pombe|c... 26 5.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 9.6
SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 25 9.6
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 68.5 bits (160), Expect = 8e-13
Identities = 30/89 (33%), Positives = 50/89 (56%)
Frame = -2
Query: 524 TETYLQMAAYFTHCKLQPVHQILTLRTALNMFFKLKNYRTAASFARRLLELGPRPEVAQQ 345
T+ L+++ YF LQP+H I+ LR A+N KLKNY++A+ +LL+L A+
Sbjct: 1068 TKRALELSYYFASADLQPMHSIIALRLAINASHKLKNYKSASFLGNKLLQLAESGPAAEA 1127
Query: 344 ARKILQACEKTPTDEHQLLYDEHNPFSVC 258
A + + ++ P D ++ YD H +C
Sbjct: 1128 ANRAITLGDRNPHDAFEIEYDPHVEMRIC 1156
Score = 37.1 bits (82), Expect = 0.002
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = -1
Query: 243 PIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGLR 115
P+Y G + CS+C A + + ++C VC V IG+ G R
Sbjct: 1162 PVYSGDDFDVCSVCGAVYHKGYVNEVCTVCDVGGIGQKGTGRR 1204
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 26.6 bits (56), Expect = 3.1
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -2
Query: 344 ARKILQACEKTPTDEHQLLYDEHNPFSVC 258
+R +L C++ D +Q+L E +P S+C
Sbjct: 369 SRGVLSFCDQAIRDLYQILEVEFHPLSIC 397
>SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 224
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -1
Query: 633 RPSCPRPNSYWPSAGTTSWDSPWRPP 556
RP+ RPNS+ P +T + SP PP
Sbjct: 76 RPTSQRPNSWQPGNASTMYASP--PP 99
>SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 377
Score = 25.8 bits (54), Expect = 5.5
Identities = 23/66 (34%), Positives = 35/66 (53%), Gaps = 4/66 (6%)
Frame = -2
Query: 461 ILTLRTALNMFFKLKNYRTAASF----ARRLLELGPRPEVAQQARKILQACEKTPTDEHQ 294
ILT+RT++ + FK+ N + AR L E G E A+ ++LQ K P +E
Sbjct: 155 ILTVRTSIPLAFKVSNDQLLMELQLCNARALDETGQFLEAAKCYYRVLQ--YKVPGNE-- 210
Query: 293 LLYDEH 276
L+Y E+
Sbjct: 211 LIYREN 216
>SPAC13A11.05 |||peptidase family M17|Schizosaccharomyces pombe|chr
1|||Manual
Length = 513
Score = 25.8 bits (54), Expect = 5.5
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +1
Query: 64 DTRF*RASLSVEL*GTDTEAERVLAD 141
D F R LSVE+ TD E +LAD
Sbjct: 346 DVIFMRNGLSVEIDNTDAEGRLILAD 371
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.0 bits (52), Expect = 9.6
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -2
Query: 575 TRHGDRQESDAEEHRRRTETYLQMAAYFTHCKLQPVHQILT 453
++HGD +E D+E+ R ++ +AA + P H + T
Sbjct: 1650 SQHGDEEEEDSEDDIRSSKDAAALAAKL-FGGMAPAHPVST 1689
>SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1842
Score = 25.0 bits (52), Expect = 9.6
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -2
Query: 392 ARRLLELGPRPEVAQQARKILQ 327
A R++E+GP P +A A++ L+
Sbjct: 42 AERIVEIGPSPTLAGMAKRTLK 63
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,962,038
Number of Sequences: 5004
Number of extensions: 33828
Number of successful extensions: 132
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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