Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021227
(657 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha... 94 8e-20
At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha... 84 9e-17
At2g47560.1 68415.m05935 zinc finger (C3HC4-type RING finger) fa... 33 0.22
At4g12500.1 68417.m01975 protease inhibitor/seed storage/lipid t... 31 0.89
At3g62690.1 68416.m07042 zinc finger (C3HC4-type RING finger) fa... 31 0.89
At4g17910.1 68417.m02669 zinc finger (C3HC4-type RING finger) fa... 29 2.1
At3g57000.1 68416.m06345 nucleolar essential protein-related con... 29 2.1
At4g12490.1 68417.m01974 protease inhibitor/seed storage/lipid t... 29 2.7
At3g52860.1 68416.m05825 expressed protein 29 3.6
At3g28790.1 68416.m03593 expressed protein 29 3.6
At4g17950.1 68417.m02673 DNA-binding family protein contains Pfa... 28 4.8
At2g17525.1 68415.m02027 pentatricopeptide (PPR) repeat-containi... 28 4.8
At1g63400.1 68414.m07170 pentatricopeptide (PPR) repeat-containi... 28 6.3
At1g62590.1 68414.m07061 pentatricopeptide (PPR) repeat-containi... 28 6.3
At5g20600.1 68418.m02446 expressed protein 27 8.3
At4g11360.1 68417.m01832 zinc finger (C3HC4-type RING finger) fa... 27 8.3
At2g43800.1 68415.m05445 formin homology 2 domain-containing pro... 27 8.3
>At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha,
putative contains Pfam PF00400: WD domain, G-beta repeat;
similar to Coatomer alpha subunit (Alpha-coat protein)
(Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621) [Homo sapiens]
Length = 1216
Score = 93.9 bits (223), Expect = 8e-20
Identities = 44/85 (51%), Positives = 58/85 (68%)
Frame = -2
Query: 509 QMAAYFTHCKLQPVHQILTLRTALNMFFKLKNYRTAASFARRLLELGPRPEVAQQARKIL 330
++AAYFTHC LQ H L L +A+ + +K KN TA++FARRLLE P A+ AR+++
Sbjct: 1078 ELAAYFTHCNLQTPHLRLALLSAMGVCYKAKNLATASNFARRLLETSPVDSQAKMARQVV 1137
Query: 329 QACEKTPTDEHQLLYDEHNPFSVCG 255
QA E+ TDE +L YD NPF VCG
Sbjct: 1138 QAAERNMTDETKLNYDFRNPFVVCG 1162
Score = 57.6 bits (133), Expect = 7e-09
Identities = 24/51 (47%), Positives = 31/51 (60%)
Frame = -1
Query: 252 SYKPIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGLRICPLQ 100
+Y PIYRG+ + C C A F+P +G +C VC +A IG DA GL P Q
Sbjct: 1164 TYVPIYRGQKDVSCPYCTARFVPNQEGNICTVCDLAVIGADASGLLCSPSQ 1214
>At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha,
putative contains Pfam PF00400: WD domain, G-beta repeat;
similar to Coatomer alpha subunit (Alpha-coat protein)
(Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621) [Homo sapiens]
Length = 1218
Score = 83.8 bits (198), Expect = 9e-17
Identities = 42/86 (48%), Positives = 55/86 (63%), Gaps = 1/86 (1%)
Frame = -2
Query: 509 QMAAYFTHCKLQPVHQILTLRTALNMFFKLKNYRTAASFARRLLELGPRPE-VAQQARKI 333
++AAYFTHCKLQ H L +A+ + +K KN TAA FAR LL+ P E A+ AR++
Sbjct: 1079 ELAAYFTHCKLQTPHLRLAYFSAMTVCYKSKNMATAAHFARSLLDTNPTIESQARTARQV 1138
Query: 332 LQACEKTPTDEHQLLYDEHNPFSVCG 255
+QA E+ TD L YD NPF +CG
Sbjct: 1139 MQAAERNMTDATTLNYDFRNPFVICG 1164
Score = 53.6 bits (123), Expect = 1e-07
Identities = 21/45 (46%), Positives = 29/45 (64%)
Frame = -1
Query: 252 SYKPIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGL 118
+Y PIY+G+ + C C A F+P +G +C VC +A IG DA GL
Sbjct: 1166 TYVPIYKGQKDVACPYCTARFVPSQEGNICSVCDLAVIGADASGL 1210
>At2g47560.1 68415.m05935 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 227
Score = 32.7 bits (71), Expect = 0.22
Identities = 14/27 (51%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
Frame = -1
Query: 225 PEEK--CSLCAASFMPEHKGKLCPVCG 151
PEEK CS+C + F E +G+L P CG
Sbjct: 102 PEEKEECSVCLSEFEEEDEGRLLPKCG 128
>At4g12500.1 68417.m01975 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to pEARLI
1 (Accession No. L43080): an Arabidopsis member of a
conserved gene family (PGF95-099), Plant Physiol. 109
(4), 1497 (1995); contains Pfam protease inhibitor/seed
storage/LTP family domain PF00234
Length = 177
Score = 30.7 bits (66), Expect = 0.89
Identities = 18/44 (40%), Positives = 21/44 (47%)
Frame = -1
Query: 636 PRPSCPRPNSYWPSAGTTSWDSPWRPPGKRCRRTPSTNRNVPAD 505
P PS P P+ PS T S SP P RTP ++ N P D
Sbjct: 55 PSPSVPTPSVPTPSVPTPSVPSP-NPTPVTPPRTPGSSGNCPID 97
>At3g62690.1 68416.m07042 zinc finger (C3HC4-type RING finger)
family protein (ATL5) identical to RING-H2 zinc finger
protein ATL5 [Arabidopsis thaliana]
gi|4928401|gb|AAD33583
Length = 257
Score = 30.7 bits (66), Expect = 0.89
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = -1
Query: 225 PEEKCSLCAASFMPEHKGKLCPVCG 151
P E+CS+C + F + +G++ P CG
Sbjct: 109 PLEECSVCLSEFEEDDEGRVLPKCG 133
>At4g17910.1 68417.m02669 zinc finger (C3HC4-type RING finger)
family protein / pentatricopeptide (PPR)
repeat-containing protein contains Pfam domains PF01535:
PPR repeat and PF00097: Zinc finger, C3HC4 type (RING
finger)
Length = 1208
Score = 29.5 bits (63), Expect = 2.1
Identities = 16/61 (26%), Positives = 28/61 (45%)
Frame = -2
Query: 461 ILTLRTALNMFFKLKNYRTAASFARRLLELGPRPEVAQQARKILQACEKTPTDEHQLLYD 282
++T T ++ + + A + RR+ + G RP+VA I A + D L+D
Sbjct: 48 VVTYNTLISGYCRFVGIEEAYAVTRRMRDAGIRPDVATYNSLIAGAARRLMLDHVLYLFD 107
Query: 281 E 279
E
Sbjct: 108 E 108
>At3g57000.1 68416.m06345 nucleolar essential protein-related
contains weak similarity to Nucleolar essential protein
1 (Essential for mitotic growth 1) (Swiss-Prot:Q06287)
[Saccharomyces cerevisiae]
Length = 298
Score = 29.5 bits (63), Expect = 2.1
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 5/64 (7%)
Frame = -2
Query: 452 LRTALNMFFKLKNY----RTAASFARRLLELGPRPEV-AQQARKILQACEKTPTDEHQLL 288
+RT + F++K + RT FA +L+L + + A +R+ L C K P +EH L
Sbjct: 159 VRTEKGVLFEVKPHVRIPRTFKRFAGIMLQLLQKLSITAVNSREKLLRCVKNPIEEHHLP 218
Query: 287 YDEH 276
+ H
Sbjct: 219 VNSH 222
>At4g12490.1 68417.m01974 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to pEARLI
1 (Accession No. L43080): an Arabidopsis member of a
conserved gene family (PGF95-099), Plant Physiol. 109
(4), 1497 (1995); contains Pfam protease inhibitor/seed
storage/LTP family domain PF00234
Length = 182
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/46 (36%), Positives = 22/46 (47%)
Frame = -1
Query: 642 WTPRPSCPRPNSYWPSAGTTSWDSPWRPPGKRCRRTPSTNRNVPAD 505
+ P PS P P+ PS + S SP P RTP ++ N P D
Sbjct: 58 YVPTPSVPSPSVPTPSVPSPSVPSP-NPTPVIPPRTPGSSGNCPID 102
>At3g52860.1 68416.m05825 expressed protein
Length = 156
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = -1
Query: 600 PSAGTTSWDSPWRPPGKRCRRTPSTNRN 517
P + S P RPPG R TPS N+N
Sbjct: 8 PQSSDPSPSPPDRPPGIRSPETPSNNQN 35
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/41 (36%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = -1
Query: 639 TPRPSCPRPNSYWPSAGTTSWDSPWRP-PGKRCRRTPSTNR 520
TP PS P P++ PS T S +P P P TP+ +
Sbjct: 280 TPTPSTPTPSTPTPSTPTPSTPTPSTPTPSTPAPSTPAAGK 320
>At4g17950.1 68417.m02673 DNA-binding family protein contains Pfam
PF03479: Domain of unknown function (DUF296); contains
Pfam PF02178: AT hook motif;
Length = 439
Score = 28.3 bits (60), Expect = 4.8
Identities = 20/66 (30%), Positives = 27/66 (40%)
Frame = -2
Query: 656 AAAAGGLQGRAVRGPTVIGRLQGLPRGTRHGDRQESDAEEHRRRTETYLQMAAYFTHCKL 477
+A A L V GP R QG + + ES++ HRR F +
Sbjct: 360 SAPANMLSFGGVGGPGS-PRSQGQQHSSESSEENESNSPLHRRSNNNNSNNHGIFGNSTP 418
Query: 476 QPVHQI 459
QP+HQI
Sbjct: 419 QPLHQI 424
>At2g17525.1 68415.m02027 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat; gene
structure supported by cDNA sequence and Brassica genome
sequence alignments.
Length = 626
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/51 (27%), Positives = 26/51 (50%)
Frame = -2
Query: 431 FFKLKNYRTAASFARRLLELGPRPEVAQQARKILQACEKTPTDEHQLLYDE 279
F+K + A F ++ +L PR ++ K++ CEK D+ + YD+
Sbjct: 435 FYKENRWEDALEFLLKMEKLFPR--AVDRSFKLISLCEKGGMDDLKTAYDQ 483
>At1g63400.1 68414.m07170 pentatricopeptide (PPR) repeat-containing
protein contains multiple PPR-repeats Pfam profile:
PF01535
Length = 577
Score = 27.9 bits (59), Expect = 6.3
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = -2
Query: 530 RRTETYLQMAAYFTHCKLQPVHQILTLRTALNMFFKLKNYRTAASFARRLLELGPRPE 357
RR++ L +A KL I+TL + LN + K A + +++E+G RP+
Sbjct: 132 RRSQISLALALLGKMMKLGYEPSIVTLSSLLNGYCHGKRISDAVALVDQMVEMGYRPD 189
>At1g62590.1 68414.m07061 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 634
Score = 27.9 bits (59), Expect = 6.3
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = -2
Query: 530 RRTETYLQMAAYFTHCKLQPVHQILTLRTALNMFFKLKNYRTAASFARRLLELGPRPE 357
RR++ L +A KL I+TL + LN + K A + +++E+G RP+
Sbjct: 132 RRSQISLALALLGKMMKLGYEPSIVTLSSLLNGYCHGKRISDAVALVDQMVEMGYRPD 189
>At5g20600.1 68418.m02446 expressed protein
Length = 532
Score = 27.5 bits (58), Expect = 8.3
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -2
Query: 395 FARRLLELGPRPEVAQQARKILQACEK 315
FA+R ELG PE Q RK++ A K
Sbjct: 261 FAKRFYELGSSPECHQGNRKLIFALHK 287
>At4g11360.1 68417.m01832 zinc finger (C3HC4-type RING finger)
family protein (RHA1b) identical to RING-H2 finger
protein RHA1b [Arabidopsis thaliana] GI:3790567
Length = 157
Score = 27.5 bits (58), Expect = 8.3
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = -1
Query: 225 PEEKCSLCAASFMPEHKGKLCPVCG 151
PE+ C++C + F+ + K + P CG
Sbjct: 81 PEDCCTVCLSDFVSDDKIRQLPKCG 105
>At2g43800.1 68415.m05445 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 894
Score = 27.5 bits (58), Expect = 8.3
Identities = 14/29 (48%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Frame = -1
Query: 636 PRPSCPRPNSYWPSAGTTSWDS--PWRPP 556
P PS P PN ++PS+ TS S P PP
Sbjct: 90 PPPSPPHPNPFFPSSDPTSTASHPPPAPP 118
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,958,112
Number of Sequences: 28952
Number of extensions: 205534
Number of successful extensions: 798
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 771
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 792
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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