BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021222 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity fac... 28 1.1 SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 28 1.5 SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosacchar... 27 2.6 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 4.5 SPCC576.14 |||diphthine synthase |Schizosaccharomyces pombe|chr ... 26 5.9 SPBC19C7.09c |uve1|uvde|endonuclease Uve1 |Schizosaccharomyces p... 26 5.9 SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.9 SPCC622.14 |||GTPase activating protein |Schizosaccharomyces pom... 25 7.9 >SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity factor complex subunit, Fip1 homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +2 Query: 380 GPSHAKGAHRTPLEAHQNGAETTRGQPAPPAHH 478 G +HA+ H P ++ NGA T PP++H Sbjct: 277 GMTHAQPTHN-PTSSYGNGASTNYNASRPPSNH 308 >SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 27.9 bits (59), Expect = 1.5 Identities = 14/47 (29%), Positives = 23/47 (48%) Frame = +2 Query: 158 ISRTPHYTRRRDVGDAETATLLPLRLAPHAVGMLHEFSEAVCRGCVN 298 ISR P + + E A L L+ P + L E+ + +CR C++ Sbjct: 254 ISRCPLNACKIAGQETELADLFSLQKVPACIYCLREWKKPICRYCID 300 >SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosaccharomyces pombe|chr 1|||Manual Length = 768 Score = 27.1 bits (57), Expect = 2.6 Identities = 15/54 (27%), Positives = 24/54 (44%), Gaps = 3/54 (5%) Frame = +3 Query: 12 LLCSGNKQLSSAKSNVKFCVFICDYHGDSVFSRRH---NKSVTIFKRNEENRSL 164 +L KQ+S + F C+Y D ++ H + +F RN EN S+ Sbjct: 391 MLAKPTKQISEVLNTFDQAAFTCEYKYDGERAQVHFTEDGKFYVFSRNSENMSV 444 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 26.2 bits (55), Expect = 4.5 Identities = 19/79 (24%), Positives = 29/79 (36%), Gaps = 1/79 (1%) Frame = +2 Query: 251 GMLHEFSEAVCRGCVNYEGADRIEVVLETARQLKRAHGFQEGRGPSHAK-GAHRTPLEAH 427 G H+ A +G ++ + V + R ++ P+H + G H P H Sbjct: 154 GKHHKGKHAKGKGKKSHPKPEDDSVFFDDERPKHHEFDDEDREFPAHHEPGEHMPPPPMH 213 Query: 428 QNGAETTRGQPAPPAHHTP 484 E P PP HH P Sbjct: 214 HKPGEH---MPPPPMHHEP 229 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = +2 Query: 389 HAKGAHRTPLEAHQNGAETTRGQPAPPAHHTP 484 H G H P H E P PP HH P Sbjct: 214 HKPGEHMPPPPMHHEPGEH---MPPPPMHHEP 242 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = +2 Query: 389 HAKGAHRTPLEAHQNGAETTRGQPAPPAHHTP 484 H G H P H E P PP HH P Sbjct: 227 HEPGEHMPPPPMHHEPGEH---MPPPPMHHEP 255 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = +2 Query: 389 HAKGAHRTPLEAHQNGAETTRGQPAPPAHHTP 484 H G H P H E P PP HH P Sbjct: 240 HEPGEHMPPPPMHHEPGEH---MPPPPMHHEP 268 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = +2 Query: 389 HAKGAHRTPLEAHQNGAETTRGQPAPPAHHTP 484 H G H P H E P PP HH P Sbjct: 253 HEPGEHMPPPPMHHEPGEH---MPPPPMHHEP 281 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = +2 Query: 389 HAKGAHRTPLEAHQNGAETTRGQPAPPAHHTP 484 H G H P H E P PP HH P Sbjct: 266 HEPGEHMPPPPMHHEPGEH---MPPPPMHHEP 294 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = +2 Query: 389 HAKGAHRTPLEAHQNGAETTRGQPAPPAHHTP 484 H G H P H E P PP HH P Sbjct: 279 HEPGEHMPPPPMHHEPGEH---MPPPPMHHEP 307 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = +2 Query: 389 HAKGAHRTPLEAHQNGAETTRGQPAPPAHHTP 484 H G H P H E P PP HH P Sbjct: 292 HEPGEHMPPPPMHHEPGEH---MPPPPMHHEP 320 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = +2 Query: 389 HAKGAHRTPLEAHQNGAETTRGQPAPPAHHTP 484 H G H P H E P PP HH P Sbjct: 305 HEPGEHMPPPPMHHEPGEH---MPPPPMHHEP 333 >SPCC576.14 |||diphthine synthase |Schizosaccharomyces pombe|chr 3|||Manual Length = 283 Score = 25.8 bits (54), Expect = 5.9 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = +2 Query: 464 PPAHHTPTVGFTLHHHDLRLVQAFWLNME 550 PP H VG +H +L ++A+ +N+E Sbjct: 243 PPLHSVVLVGRDVHDLELEFLRAYAVNLE 271 >SPBC19C7.09c |uve1|uvde|endonuclease Uve1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 599 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/33 (39%), Positives = 15/33 (45%) Frame = -3 Query: 475 VSWRCRLSSCCFCSILMGF*GRTVCSFCMRRTT 377 + WR RL C +IL R CS R TT Sbjct: 244 IPWRGRLGYACLNTILRSMKERVFCSRTCRITT 276 >SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1379 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/62 (24%), Positives = 29/62 (46%) Frame = +3 Query: 27 NKQLSSAKSNVKFCVFICDYHGDSVFSRRHNKSVTIFKRNEENRSLALRTTPGGEMSVMQ 206 ++Q SA + +CD H S FS ++ + + +E S+ L +PG +S+ Sbjct: 541 SQQTESASKSSSNISEMCDSHPPSNFSISASQQASKDRFSEATSSIDLDLSPGRSLSLAS 600 Query: 207 KR 212 + Sbjct: 601 SK 602 >SPCC622.14 |||GTPase activating protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 309 Score = 25.4 bits (53), Expect = 7.9 Identities = 7/24 (29%), Positives = 14/24 (58%) Frame = +3 Query: 189 EMSVMQKRQHCYLCDLPRMPWACC 260 +++ + + + C+ CD P WA C Sbjct: 7 QLTRLPENKKCFDCDAPNPQWASC 30 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,551,565 Number of Sequences: 5004 Number of extensions: 49019 Number of successful extensions: 142 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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