Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021216
(688 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 38 0.001
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 37 0.003
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 36 0.004
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 34 0.022
SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 30 0.27
SPBC16H5.11c |skb1|rmt5|type II protein arginine N-methyltransfe... 30 0.36
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 27 1.9
SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|ch... 27 2.5
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 27 2.5
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 27 3.4
SPAPJ696.02 |||actin cortical patch component Lsb4 |Schizosaccha... 26 5.9
SPAC1039.01 |||amino acid permease, unknown 5|Schizosaccharomyce... 26 5.9
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 7.8
SPAC1B3.07c |vps28||ESCRT I complex subunit Vps28|Schizosaccharo... 25 7.8
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 25 7.8
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 37.9 bits (84), Expect = 0.001
Identities = 13/25 (52%), Positives = 20/25 (80%)
Frame = +2
Query: 293 NWYRAELDGKEGLIPSNYIQMKNHS 367
+W+R EL+GKEG+ PSNY+++ S
Sbjct: 176 DWWRGELNGKEGIFPSNYVRLLEDS 200
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 36.7 bits (81), Expect = 0.003
Identities = 12/37 (32%), Positives = 24/37 (64%)
Frame = +2
Query: 251 KNQVLKILNMEDDMNWYRAELDGKEGLIPSNYIQMKN 361
+ Q +L +D W R ++DG +GLIP++Y+++ +
Sbjct: 538 EGQEFTLLEPDDGSGWVRVKIDGTDGLIPASYVKLND 574
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 36.3 bits (80), Expect = 0.004
Identities = 13/33 (39%), Positives = 23/33 (69%)
Frame = +2
Query: 254 NQVLKILNMEDDMNWYRAELDGKEGLIPSNYIQ 352
N + I+N +W++ +DGKEG++PSN+I+
Sbjct: 532 NMRVVIVNDTASSDWWKCSVDGKEGVVPSNFIK 564
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 33.9 bits (74), Expect = 0.022
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +2
Query: 251 KNQVLKILNMEDDMNWYRAELDGKEGLIPSNYIQM 355
+N+ ++IL DD W G++GL PSNY+++
Sbjct: 748 ENEQIQILEFVDDGWWLGENSKGQQGLFPSNYVEI 782
Score = 32.3 bits (70), Expect = 0.068
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2
Query: 287 DMNWYRAELDGKEGLIPSNYIQ 352
D NW+ E G GL PSNY++
Sbjct: 834 DPNWWEGECHGHRGLFPSNYVE 855
>SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 466
Score = 30.3 bits (65), Expect = 0.27
Identities = 10/33 (30%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = +2
Query: 263 LKILNMEDDMN-WYRAELDGKEGLIPSNYIQMK 358
+++++ D+ N W+ L+G +G P NY+Q++
Sbjct: 434 IEVVSRTDNQNEWWIGRLNGAQGQFPGNYVQLE 466
>SPBC16H5.11c |skb1|rmt5|type II protein arginine
N-methyltransferase Skb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 645
Score = 29.9 bits (64), Expect = 0.36
Identities = 20/54 (37%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +2
Query: 353 MKNHSWYYGRITRADAEKLLANKPEGGFLIRISE--SSPGDFSLSVKCPDGVQH 508
M+N + G++T + N P+ I +SE S GD LS +C DGVQH
Sbjct: 390 MRNRQDWAGKVTLVFGDMRTWN-PDYKIDILVSELLGSMGDNELSPECLDGVQH 442
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 27.5 bits (58), Expect = 1.9
Identities = 14/29 (48%), Positives = 19/29 (65%), Gaps = 2/29 (6%)
Frame = +1
Query: 574 ELVDYHRTASVSRL--QDVKLRDVVPEKC 654
EL D + +S+ L QD KLR++V EKC
Sbjct: 535 ELYDNDKLSSIDALLIQDSKLREMVSEKC 563
>SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 979
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/43 (30%), Positives = 21/43 (48%)
Frame = +2
Query: 44 NASFLFNIVRQYVHYDYFTSLTLDILESLCTLRVIFKNSVTFQ 172
N L +I + Y + F L D+L+ LCT+ + +FQ
Sbjct: 676 NEPILLDIPQNYDNMSSFKPLAHDVLKKLCTIILFHAGFESFQ 718
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 27.1 bits (57), Expect = 2.5
Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = +1
Query: 433 VLDKDQRIKPW*LL--LISQMSGRCPALKVLRDASSKFFL 546
VLDK PW L L Q R P+L +LRD S+ F+
Sbjct: 1598 VLDKSVHQFPWESLPCLNRQSVSRVPSLSILRDILSQSFV 1637
>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
Msa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 533
Score = 26.6 bits (56), Expect = 3.4
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = -2
Query: 621 VLKATDRRGTMIIN*FVERIELDDPKEELARGVS*YFECWTPSGHL 484
VL + RG + FV + +EEL V YF+ W P H+
Sbjct: 67 VLNPSSVRGKPVACLFVASLNSSRSEEELTATVKDYFQQWGPLLHV 112
>SPAPJ696.02 |||actin cortical patch component Lsb4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 25.8 bits (54), Expect = 5.9
Identities = 9/38 (23%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +2
Query: 245 F*KNQVLKILNMEDDMN-WYRAELDGKEGLIPSNYIQM 355
F K ++ I+ + W+ + +EG+ P+NY+++
Sbjct: 392 FQKGDIIDIVERSGSHDDWWTGRIGYREGIFPANYVKL 429
>SPAC1039.01 |||amino acid permease, unknown 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 567
Score = 25.8 bits (54), Expect = 5.9
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +1
Query: 235 MSSVLEKSSIKNSKHGGRYE 294
MSS++EK+ + +SKH +E
Sbjct: 1 MSSIMEKTDVSSSKHSFTFE 20
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.4 bits (53), Expect = 7.8
Identities = 19/60 (31%), Positives = 29/60 (48%)
Frame = -3
Query: 281 PCLEFLILDFSKTELIICGCSKIVFC*SLHDQIRHFTEMSPNS*K*LLKYTNFPKCLMSR 102
P L L+ FSK + I + + +D IRHF ++ K + +TN P C +SR
Sbjct: 2311 PSLWNLLNQFSKFLIEIASANSNIVYKLSYDVIRHFLKLVV-LWKNIYVWTNVPDCDISR 2369
>SPAC1B3.07c |vps28||ESCRT I complex subunit
Vps28|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 25.4 bits (53), Expect = 7.8
Identities = 14/44 (31%), Positives = 22/44 (50%)
Frame = +1
Query: 442 KDQRIKPW*LLLISQMSGRCPALKVLRDASSKFFLWVVKFNSLN 573
KDQ + P LI M +LK+ +K W++K N++N
Sbjct: 178 KDQ-LHPLLSELIVSMDDLTESLKIQVSCRNKLVQWLIKINNMN 220
>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1254
Score = 25.4 bits (53), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 492 RTVSSTQSIKRRLEQVLPLGRQVQFAQRTS*LSSY 596
RT + ++R L QVL G V A TS LS+Y
Sbjct: 468 RTQKEEKVVQRGLTQVLTSGTLVDEAMLTSDLSTY 502
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,669,393
Number of Sequences: 5004
Number of extensions: 51221
Number of successful extensions: 146
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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