BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021207 (537 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 177 1e-45 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 120 1e-28 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 120 1e-28 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 96 3e-21 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.8 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.3 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 3.1 SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 3.1 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 4.1 SPCC11E10.07c |||translation initiation factor eIF2B alpha subun... 26 4.1 SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 5.4 SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 25 5.4 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 5.4 SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3 |Schi... 25 7.2 SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosacc... 25 9.5 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 177 bits (430), Expect = 1e-45 Identities = 78/94 (82%), Positives = 87/94 (92%) Frame = +1 Query: 256 GKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSV 435 GKYVPRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+V Sbjct: 57 GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116 Query: 436 LDVVRKEAESCDCLQGFQLTHSLGVGTGSGMGTL 537 LDVVR+EAE+CD LQGFQLTHSLG GTGSGMGTL Sbjct: 117 LDVVRREAEACDALQGFQLTHSLGGGTGSGMGTL 150 Score = 93.1 bits (221), Expect = 2e-20 Identities = 38/53 (71%), Positives = 44/53 (83%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNE 247 MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NE Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNE 53 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 120 bits (290), Expect = 1e-28 Identities = 51/95 (53%), Positives = 71/95 (74%) Frame = +1 Query: 253 RGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDS 432 +GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DS Sbjct: 62 QGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDS 121 Query: 433 VLDVVRKEAESCDCLQGFQLTHSLGVGTGSGMGTL 537 VL+ +R+ A++C LQGF + HS G GTGSG+G L Sbjct: 122 VLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGAL 156 Score = 42.7 bits (96), Expect = 3e-05 Identities = 21/53 (39%), Positives = 30/53 (56%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNE 247 MRE++ + GQ G QIG WE+ EHGI P G +S ++ + N Y N+ Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENS--EVHKNNSYLND 51 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 120 bits (289), Expect = 1e-28 Identities = 52/95 (54%), Positives = 68/95 (71%) Frame = +1 Query: 253 RGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDS 432 +GKYVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD Sbjct: 58 QGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDE 117 Query: 433 VLDVVRKEAESCDCLQGFQLTHSLGVGTGSGMGTL 537 V D +R+ A++C LQGF + HS G GTGSG G L Sbjct: 118 VTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGAL 152 Score = 44.4 bits (100), Expect = 1e-05 Identities = 19/34 (55%), Positives = 21/34 (61%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG 190 MREI+ I GQ G QIG WE+ EHGI P G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 95.9 bits (228), Expect = 3e-21 Identities = 39/94 (41%), Positives = 66/94 (70%), Gaps = 2/94 (2%) Frame = +1 Query: 259 KYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSG--AGNNWAKGHYTEGAELVDS 432 +Y+PRAIL+DLEP ++++ S +G ++ P+N + ++G AGNNWA G Y+ + + Sbjct: 59 RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFED 117 Query: 433 VLDVVRKEAESCDCLQGFQLTHSLGVGTGSGMGT 534 ++D++ +EA+ D L+GF L HS+ GTGSG+G+ Sbjct: 118 IMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGS 151 Score = 56.0 bits (129), Expect = 3e-09 Identities = 23/50 (46%), Positives = 35/50 (70%) Frame = +2 Query: 92 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYY 241 REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFF 52 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 1.8 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 128 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 229 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 2.3 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +1 Query: 322 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 423 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 26.2 bits (55), Expect = 3.1 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +1 Query: 391 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 480 A+GH G ELV + D +RK++E+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 26.2 bits (55), Expect = 3.1 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = -2 Query: 464 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 327 ++A+ RTTS T+ +PS L P P + S R+ CPK Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.8 bits (54), Expect = 4.1 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +1 Query: 343 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 474 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 >SPCC11E10.07c |||translation initiation factor eIF2B alpha subunit|Schizosaccharomyces pombe|chr 3|||Manual Length = 341 Score = 25.8 bits (54), Expect = 4.1 Identities = 8/13 (61%), Positives = 11/13 (84%) Frame = -2 Query: 530 PIPEPVPTPSECV 492 P PEP+PTPS+ + Sbjct: 279 PEPEPIPTPSDAI 291 >SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 867 Score = 25.4 bits (53), Expect = 5.4 Identities = 14/32 (43%), Positives = 15/32 (46%) Frame = -2 Query: 347 GRKICPKGPERTESMVPGSKSTRMARGTYLPR 252 G I PER + KS RMAR LPR Sbjct: 106 GNNINYPTPERLQKSSASRKSGRMARSQELPR 137 >SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schizosaccharomyces pombe|chr 2|||Manual Length = 766 Score = 25.4 bits (53), Expect = 5.4 Identities = 15/46 (32%), Positives = 27/46 (58%) Frame = -3 Query: 241 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 104 ++Y+DA L +++ + G A V DLP L +D V++L S+ + Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 5.4 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -2 Query: 380 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 285 P+ PK L R I P GPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 25.0 bits (52), Expect = 7.2 Identities = 17/39 (43%), Positives = 22/39 (56%) Frame = -1 Query: 429 IDELSTLRVVSLGPVVTGAGLSEDEVVRTEDLSERSRAD 313 +D +LR VSL T AGLS DEV + S+ S A+ Sbjct: 369 LDFCDSLRRVSLHVDPTLAGLSPDEVENFYNKSKNSLAN 407 >SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosaccharomyces pombe|chr 3|||Manual Length = 1014 Score = 24.6 bits (51), Expect = 9.5 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -1 Query: 372 GLSEDEVVRTEDLSERSRADRVHGAGLQVDEDG 274 G+S E+ R +D S+ R +RVH DEDG Sbjct: 240 GISGFEINRQKDTSDMKRNNRVH----MDDEDG 268 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,202,823 Number of Sequences: 5004 Number of extensions: 42283 Number of successful extensions: 144 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 222442660 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -