BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021205X (625 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 166 3e-42 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 165 6e-42 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 145 4e-36 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 116 3e-27 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 83 3e-17 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 61 1e-10 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 46 6e-06 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.025 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.72 SPBC13A2.04c |||PTR family peptide transporter|Schizosaccharomyc... 27 2.2 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 6.7 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 6.7 SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 25 8.9 SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 8.9 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 166 bits (403), Expect = 3e-42 Identities = 99/195 (50%), Positives = 121/195 (62%), Gaps = 5/195 (2%) Frame = +1 Query: 55 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 234 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 235 TIFDAKRSSDVSXKMLLCNPT*XTGLSR--LSVMEANLRSR*HIRVK--TKPFSPRK-SV 399 TIFDAKR + K +P + + V+ + + + K TK F+P + S Sbjct: 64 TIFDAKRL--IGRK--FDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119 Query: 400 HGAYENXGNCRSLSRQNCAECSYHGSRVLQ*LSKTSPKDAGTISGLNVLRIINEPTAAAI 579 + + + + + KDAG I+GLNVLRIINEPTAAAI Sbjct: 120 MVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAI 179 Query: 580 AYGLDKKGTGERNVL 624 AYGLD+ GE NVL Sbjct: 180 AYGLDRSNQGESNVL 194 Score = 77.0 bits (181), Expect = 2e-15 Identities = 34/54 (62%), Positives = 40/54 (74%) Frame = +3 Query: 255 LIGRKXEDATVQSDMXHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSPWCLRK 416 LIGRK +D VQSDM HWPF+V+S GKP ++V YKGE KTF PEE+S L K Sbjct: 71 LIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMK 124 Score = 65.3 bits (152), Expect = 7e-12 Identities = 32/44 (72%), Positives = 34/44 (77%) Frame = +2 Query: 383 PRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514 P SMVL KM ETAEAYLG V +AV+TVPAYFNDSQRQA K Sbjct: 114 PEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATK 157 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 165 bits (400), Expect = 6e-42 Identities = 98/195 (50%), Positives = 122/195 (62%), Gaps = 5/195 (2%) Frame = +1 Query: 55 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 234 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 235 TIFDAKRSSDVSXKMLLCNPT*XTGLSR--LSVMEANLRSR*HIRVK--TKPFSPRK-SV 399 TIFDAKR + + +P + + V+E + + + K TK F+P + S Sbjct: 64 TIFDAKRL--IGRR--FNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119 Query: 400 HGAYENXGNCRSLSRQNCAECSYHGSRVLQ*LSKTSPKDAGTISGLNVLRIINEPTAAAI 579 + + + + + + KDAG I+GLNVLRIINEPTAAAI Sbjct: 120 MVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAI 179 Query: 580 AYGLDKKGTGERNVL 624 AYGLD+ E NVL Sbjct: 180 AYGLDRSNQHETNVL 194 Score = 72.1 bits (169), Expect = 6e-14 Identities = 32/54 (59%), Positives = 38/54 (70%) Frame = +3 Query: 255 LIGRKXEDATVQSDMXHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSPWCLRK 416 LIGR+ D VQSDM HWPF+V+ GKP I+V +KGE KTF PEE+S L K Sbjct: 71 LIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLK 124 Score = 62.1 bits (144), Expect = 6e-11 Identities = 30/44 (68%), Positives = 34/44 (77%) Frame = +2 Query: 383 PRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514 P SMVL KM E+AEA+LG V +AV+TVPAYFNDSQRQA K Sbjct: 114 PEEISSMVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATK 157 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 145 bits (352), Expect = 4e-36 Identities = 88/194 (45%), Positives = 114/194 (58%), Gaps = 5/194 (2%) Frame = +1 Query: 58 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 237 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP NT Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97 Query: 238 IFDAKRSSDVSXKMLLCNPT*XTGLSRLSVMEANLRSR*HIRV----KTKPFSPRK-SVH 402 IFD KR + K T + N ++R + V K K F+P + S Sbjct: 98 IFDIKRL--IGRK--FDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153 Query: 403 GAYENXGNCRSLSRQNCAECSYHGSRVLQ*LSKTSPKDAGTISGLNVLRIINEPTAAAIA 582 + + + + + KDAGTI+GLNV+RI+NEPTAAAIA Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIA 213 Query: 583 YGLDKKGTGERNVL 624 YGLDK T E++++ Sbjct: 214 YGLDKTDT-EKHIV 226 Score = 66.1 bits (154), Expect = 4e-12 Identities = 30/51 (58%), Positives = 39/51 (76%) Frame = +2 Query: 362 G*RQNLFPRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514 G ++ P +M+L+KM +TAEAYLGK V +AV+TVPAYFND+QRQA K Sbjct: 140 GKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190 Score = 50.8 bits (116), Expect = 2e-07 Identities = 23/64 (35%), Positives = 37/64 (57%) Frame = +3 Query: 225 PQQHNIRCQTLIGRKXEDATVQSDMXHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSPW 404 P+ + LIGRK ++ T+ D+ +PF +V+D +P ++V G+ K F PEE+S Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153 Query: 405 CLRK 416 L K Sbjct: 154 ILSK 157 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 116 bits (279), Expect = 3e-27 Identities = 52/67 (77%), Positives = 59/67 (88%) Frame = +1 Query: 55 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 234 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67 Query: 235 TIFDAKR 255 T+FDAKR Sbjct: 68 TVFDAKR 74 Score = 63.3 bits (147), Expect = 3e-11 Identities = 30/42 (71%), Positives = 34/42 (80%) Frame = +1 Query: 499 KTSPKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 624 + + KDAG I+GLNVLRIINEPTAAAIAYGLD K +NVL Sbjct: 157 RAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVL 198 Score = 60.5 bits (140), Expect = 2e-10 Identities = 27/68 (39%), Positives = 39/68 (57%) Frame = +3 Query: 225 PQQHNIRCQTLIGRKXEDATVQSDMXHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSPW 404 P+ + LIGR+ ED Q D+ HWPF+V+ + G P I+V Y GE K F +E+S Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124 Query: 405 CLRKXXKL 428 L K ++ Sbjct: 125 VLTKMKEI 132 Score = 55.2 bits (127), Expect = 7e-09 Identities = 27/39 (69%), Positives = 31/39 (79%) Frame = +2 Query: 398 SMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514 +MVLTKM E +EA L K V+ AVITVPAYF+DSQR A K Sbjct: 123 AMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATK 161 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 83.0 bits (196), Expect = 3e-17 Identities = 39/71 (54%), Positives = 49/71 (69%), Gaps = 1/71 (1%) Frame = +1 Query: 46 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 222 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q + Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 223 NPNNTIFDAKR 255 NP NT F KR Sbjct: 109 NPENTFFATKR 119 Score = 62.9 bits (146), Expect = 4e-11 Identities = 28/37 (75%), Positives = 32/37 (86%) Frame = +2 Query: 404 VLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514 +L+KM ETA YLGK V+NAV+TVPAYFNDSQRQA K Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATK 203 Score = 55.6 bits (128), Expect = 5e-09 Identities = 24/34 (70%), Positives = 30/34 (88%) Frame = +1 Query: 499 KTSPKDAGTISGLNVLRIINEPTAAAIAYGLDKK 600 + + K AG I+GLNVLR++NEPTAAA+AYGLDKK Sbjct: 199 RQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK 232 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 61.3 bits (142), Expect = 1e-10 Identities = 52/196 (26%), Positives = 91/196 (46%), Gaps = 11/196 (5%) Frame = +1 Query: 43 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 222 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62 Query: 223 NPNNTI----------FDAKRSSDVSXKMLLCNPT*XTGLSRLSVMEANLRSR*-HIRVK 369 N NT+ +D D+ + T G V N + +I++ Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122 Query: 370 TKPFSPRKSVHGAYENXGNCRSLSRQNCAECSYHGSRVLQ*LSKTSPKDAGTISGLNVLR 549 F+ K++ A E G+ + A + R L +A I+GLN LR Sbjct: 123 AAYFTKIKAIAEA-ELIGSVSDVVISIPAWFTDIQRRALL--------EAANIAGLNPLR 173 Query: 550 IINEPTAAAIAYGLDK 597 ++N+ AAA+ YG+ K Sbjct: 174 LMNDNAAAALTYGITK 189 Score = 33.5 bits (73), Expect = 0.025 Identities = 16/33 (48%), Positives = 23/33 (69%) Frame = +2 Query: 410 TKMXETAEAYLGKTVQNAVITVPAYFNDSQRQA 508 TK+ AEA L +V + VI++PA+F D QR+A Sbjct: 127 TKIKAIAEAELIGSVSDVVISIPAWFTDIQRRA 159 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 45.6 bits (103), Expect = 6e-06 Identities = 18/61 (29%), Positives = 35/61 (57%) Frame = +1 Query: 58 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 237 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N +N+ Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86 Query: 238 I 240 + Sbjct: 87 V 87 Score = 38.7 bits (86), Expect = 7e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 407 LTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQA 508 L ++ E+AE +LG V V++VP YF D+QR+A Sbjct: 148 LRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKA 181 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 33.5 bits (73), Expect = 0.025 Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 1/72 (1%) Frame = +1 Query: 58 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 234 + ID GT ++ + + G +EI+ R S VAF ER+ G A N P + Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPAH 84 Query: 235 TIFDAKRSSDVS 270 +I + K D + Sbjct: 85 SIRNVKELLDTA 96 Score = 25.8 bits (54), Expect = 5.1 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = +2 Query: 398 SMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQR 502 +M + AE + + + V+TVP +FN+ QR Sbjct: 142 AMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQR 176 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.72 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +2 Query: 50 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 145 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPBC13A2.04c |||PTR family peptide transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 27.1 bits (57), Expect = 2.2 Identities = 17/52 (32%), Positives = 25/52 (48%), Gaps = 1/52 (1%) Frame = -1 Query: 400 GLTSSGKKVLSSPLYATLILGLPPSLTTSK-GQXIMSDCTVASSXLRPMSVW 248 GL + G K SPL A + +PP + T K G ++ D V +S + W Sbjct: 201 GLGTGGIKSNVSPLMAEQLPKIPPYVKTKKNGSKVIVDPVVTTSRAYMIFYW 252 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 25.4 bits (53), Expect = 6.7 Identities = 17/66 (25%), Positives = 31/66 (46%) Frame = +3 Query: 36 QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 215 +NGK T +R +S + W P++E R G D + V R + + +EP Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531 Query: 216 GDEPQQ 233 ++P++ Sbjct: 532 -EKPEE 536 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.4 bits (53), Expect = 6.7 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = -1 Query: 136 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 32 G W SP S + H ++++ +F +RVL C+ Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310 >SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 25.0 bits (52), Expect = 8.9 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +1 Query: 196 DAAKNQVAMNPNNTIFDAKRSSDVSXKMLLCNPT*XTGLSRLSV 327 D + + +NP I++A + S + L+C +GL RL V Sbjct: 387 DTEEPEAQLNPKRKIWEAIQPGFTSGEDLICGYKDESGLHRLFV 430 >SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 475 Score = 25.0 bits (52), Expect = 8.9 Identities = 9/25 (36%), Positives = 14/25 (56%) Frame = +3 Query: 360 KGEDKTFFPEEVSPWCLRKXXKLPK 434 K + + + P V+ W LRK +PK Sbjct: 67 KSKSEDYQPSNVNVWALRKEKMIPK 91 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,686,519 Number of Sequences: 5004 Number of extensions: 55544 Number of successful extensions: 182 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 181 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 275671126 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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