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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021205X
         (625 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)                      188   3e-48
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)                      151   4e-37
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)             146   1e-35
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)                       143   9e-35
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)                 81   9e-16
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)              54   9e-08
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)              50   2e-06
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)                 42   5e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)                 31   0.76 
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   0.76 
SB_56988| Best HMM Match : DUF229 (HMM E-Value=2e-06)                  30   1.3  
SB_4173| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   1.3  
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)            29   2.3  
SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37)                   29   3.1  
SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9)                   29   3.1  
SB_53143| Best HMM Match : PKD (HMM E-Value=2.7e-18)                   28   7.1  
SB_30440| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.1  

>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
          Length = 1097

 Score =  188 bits (458), Expect = 3e-48
 Identities = 116/205 (56%), Positives = 134/205 (65%), Gaps = 10/205 (4%)
 Frame = +1

Query: 40  MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 219
           MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1   MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60

Query: 220 MNPNNTIFDAKRSSDVSXKMLLCNPT*XTGLSRLSVMEANLRSR*HIRVKTKPFSPRKSV 399
           MNP NT+FDAKR   +  K    +P     ++           R  I+V+ K    +KS 
Sbjct: 61  MNPENTVFDAKRL--IGRK--FDDPVVARDMTHWPFHVIREGERPKIQVEFK--GEKKSF 114

Query: 400 HGAYENXGNCRSLSRQNCAECSYHGSRVLQ*L----------SKTSPKDAGTISGLNVLR 549
               E   +      +  AE +Y G++V   +           + + KDAGTI+GLNVLR
Sbjct: 115 FP--EEISSMVLTKMKETAE-AYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLR 171

Query: 550 IINEPTAAAIAYGLDKKGTGERNVL 624
           IINEPTAAAIAYGLDKK   ERNVL
Sbjct: 172 IINEPTAAAIAYGLDKKVGVERNVL 196



 Score = 74.5 bits (175), Expect = 6e-14
 Identities = 32/64 (50%), Positives = 42/64 (65%)
 Frame = +3

Query: 225 PQQHNIRCQTLIGRKXEDATVQSDMXHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSPW 404
           P+      + LIGRK +D  V  DM HWPF V+ +G +PKI+V +KGE K+FFPEE+S  
Sbjct: 63  PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122

Query: 405 CLRK 416
            L K
Sbjct: 123 VLTK 126



 Score = 70.9 bits (166), Expect = 8e-13
 Identities = 34/51 (66%), Positives = 40/51 (78%)
 Frame = +2

Query: 362 G*RQNLFPRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514
           G +++ FP    SMVLTKM ETAEAYLG  V +AV+TVPAYF+DSQRQA K
Sbjct: 109 GEKKSFFPEEISSMVLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATK 159


>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
          Length = 1327

 Score =  151 bits (366), Expect = 4e-37
 Identities = 96/200 (48%), Positives = 121/200 (60%), Gaps = 11/200 (5%)
 Frame = +1

Query: 58   VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 234
            +GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+  ERLIGD AKNQ+  NP N
Sbjct: 704  IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763

Query: 235  TIFDAKRSSDVSXKMLLCNPT*XTGLSRLSVMEANLRSR*HIRVKTKPFSPRKSVHGAYE 414
            T+FDAKR    S      + +    L        +   + H++VK K     K+   A E
Sbjct: 764  TVFDAKRLIGRSWD----DTSVQNDLKFFPFKVVDKNKKPHVQVKVK--DEIKTF--AAE 815

Query: 415  NXGNCRSLSRQNCAECSYHGSRVLQ*L----------SKTSPKDAGTISGLNVLRIINEP 564
                      +  AE +Y G +V   +           + + KDAG I+GL V+RIINEP
Sbjct: 816  EISAMVLTKMKETAE-AYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGLTVMRIINEP 874

Query: 565  TAAAIAYGLDKKGTGERNVL 624
            TAAAIAYG+DKK  GE+N+L
Sbjct: 875  TAAAIAYGMDKK-EGEKNIL 893



 Score = 66.9 bits (156), Expect = 1e-11
 Identities = 31/39 (79%), Positives = 35/39 (89%)
 Frame = +2

Query: 398 SMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514
           +MVLTKM ETAEAYLGK V +AV+TVPAYFND+QRQA K
Sbjct: 819 AMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATK 857



 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 26/64 (40%), Positives = 37/64 (57%)
 Frame = +3

Query: 225 PQQHNIRCQTLIGRKXEDATVQSDMXHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSPW 404
           P+      + LIGR  +D +VQ+D+  +PF+VV    KP ++V  K E KTF  EE+S  
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820

Query: 405 CLRK 416
            L K
Sbjct: 821 VLTK 824


>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 746

 Score =  146 bits (355), Expect = 1e-35
 Identities = 67/72 (93%), Positives = 69/72 (95%)
 Frame = +1

Query: 40  MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 219
           M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1   MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60

Query: 220 MNPNNTIFDAKR 255
           MNP NTIFDAKR
Sbjct: 61  MNPTNTIFDAKR 72



 Score = 71.7 bits (168), Expect = 4e-13
 Identities = 30/47 (63%), Positives = 37/47 (78%)
 Frame = +3

Query: 255 LIGRKXEDATVQSDMXHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV 395
           LIGR+ +D  V+ DM HW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 73  LIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEV 119



 Score = 68.5 bits (160), Expect = 4e-12
 Identities = 34/42 (80%), Positives = 36/42 (85%)
 Frame = +1

Query: 499 KTSPKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 624
           + + KDAG ISGLNVLRIINEPTAAAIAYGLDKK   ERNVL
Sbjct: 248 RQATKDAGVISGLNVLRIINEPTAAAIAYGLDKKVGQERNVL 289



 Score = 65.3 bits (152), Expect = 4e-11
 Identities = 33/51 (64%), Positives = 36/51 (70%)
 Frame = +2

Query: 362 G*RQNLFPRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514
           G  +  F     SMVL KM ETAEAYLG  V +AV+TVPAYFNDSQRQA K
Sbjct: 134 GETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATK 184



 Score = 64.1 bits (149), Expect = 9e-11
 Identities = 31/39 (79%), Positives = 33/39 (84%)
 Frame = +2

Query: 398 SMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514
           SMVL KM ETAEAYLG  V +AV+TVPAYFNDSQRQA K
Sbjct: 214 SMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATK 252


>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
          Length = 640

 Score =  143 bits (347), Expect = 9e-35
 Identities = 65/72 (90%), Positives = 69/72 (95%)
 Frame = +1

Query: 40  MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 219
           M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1   MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60

Query: 220 MNPNNTIFDAKR 255
           +NP NTIFDAKR
Sbjct: 61  LNPENTIFDAKR 72



 Score = 76.6 bits (180), Expect = 2e-14
 Identities = 35/64 (54%), Positives = 44/64 (68%)
 Frame = +3

Query: 225 PQQHNIRCQTLIGRKXEDATVQSDMXHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSPW 404
           P+      + LIGR+ +DATVQSDM  WPF+++SD  KPKI+V YKGE KTF  EE+S  
Sbjct: 63  PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122

Query: 405 CLRK 416
            L K
Sbjct: 123 VLGK 126



 Score = 68.9 bits (161), Expect = 3e-12
 Identities = 31/42 (73%), Positives = 38/42 (90%)
 Frame = +1

Query: 499 KTSPKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 624
           + + KDAGTI+GLNVLR+INEPTAAA+AYGLDK  +GE+NVL
Sbjct: 155 RQATKDAGTIAGLNVLRVINEPTAAALAYGLDKSLSGEKNVL 196



 Score = 65.7 bits (153), Expect = 3e-11
 Identities = 34/51 (66%), Positives = 37/51 (72%)
 Frame = +2

Query: 362 G*RQNLFPRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514
           G R+        SMVL KM ETAEAYLG+ V +AVITVPAYFNDSQRQA K
Sbjct: 109 GERKTFAAEEISSMVLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATK 159


>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
          Length = 437

 Score = 80.6 bits (190), Expect = 9e-16
 Identities = 36/72 (50%), Positives = 51/72 (70%), Gaps = 2/72 (2%)
 Frame = +1

Query: 46  KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 219
           K   +GIDLGTTYSCVG +Q   G+++I +++  ++  PS VAFTD   L+G+ A +Q  
Sbjct: 30  KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89

Query: 220 MNPNNTIFDAKR 255
           +NP NTI+DAKR
Sbjct: 90  INPKNTIYDAKR 101



 Score = 46.8 bits (106), Expect = 1e-05
 Identities = 22/36 (61%), Positives = 29/36 (80%)
 Frame = +1

Query: 517 AGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 624
           A +++G+ VLR+INEPTAAA+AYGL KK  G  NV+
Sbjct: 191 AASLAGITVLRVINEPTAAALAYGLHKK-EGVANVM 225


>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
          Length = 179

 Score = 54.0 bits (124), Expect = 9e-08
 Identities = 25/51 (49%), Positives = 37/51 (72%)
 Frame = +2

Query: 362 G*RQNLFPRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 514
           G +++  P    ++VL ++   AEA+LG+ V+ AVITVPA+FND+QRQA K
Sbjct: 8   GEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATK 58



 Score = 44.0 bits (99), Expect = 1e-04
 Identities = 23/28 (82%), Positives = 23/28 (82%), Gaps = 2/28 (7%)
 Frame = +1

Query: 541 VLRIINEPTAAAIAYGLDKK--GTGERN 618
           VLRIINEPTAAAIAYGLDK   G  ERN
Sbjct: 92  VLRIINEPTAAAIAYGLDKARGGKAERN 119


>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1068

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 22/52 (42%), Positives = 33/52 (63%)
 Frame = +1

Query: 58  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 213
           VG D+G     + V + G +E +AN+  +R TPS+V+  D +RLIG + KNQ
Sbjct: 4   VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55



 Score = 38.3 bits (85), Expect = 0.005
 Identities = 19/47 (40%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
 Frame = +3

Query: 258 IGRKXEDATVQSDMXHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEV 395
           IGRK  D  VQ ++ H P++VV        I+V Y G+ + F PE+V
Sbjct: 130 IGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQV 176



 Score = 37.1 bits (82), Expect = 0.012
 Identities = 18/47 (38%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
 Frame = +2

Query: 368 RQNLF-PRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQ 505
           +Q +F P    +M+ T++  TAE  L   V + VI+VP+Y+ D QR+
Sbjct: 167 KQEVFSPEQVMAMLFTRLKTTAEIALKTKVTDCVISVPSYYTDRQRR 213



 Score = 35.5 bits (78), Expect = 0.035
 Identities = 14/29 (48%), Positives = 22/29 (75%)
 Frame = +1

Query: 514 DAGTISGLNVLRIINEPTAAAIAYGLDKK 600
           DA   +GLN LR++N+ TA ++AYG+ K+
Sbjct: 217 DASATAGLNCLRLMNDTTAVSLAYGIYKQ 245


>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
          Length = 592

 Score = 41.5 bits (93), Expect = 5e-04
 Identities = 18/28 (64%), Positives = 22/28 (78%)
 Frame = +1

Query: 109 GKVEIIANDQGNRTTPSYVAFTDTERLI 192
           G   IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83  GNTTIIANDAGDRVTPAMVAFSDTEKLI 110


>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
          Length = 212

 Score = 31.1 bits (67), Expect = 0.76
 Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
 Frame = +1

Query: 118 EIIANDQGNRTTPSYVAFT-DTERL 189
           ++I N +G+RTTPS VAFT + ERL
Sbjct: 7   KVIENAEGSRTTPSVVAFTPEGERL 31


>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1019

 Score = 31.1 bits (67), Expect = 0.76
 Identities = 14/25 (56%), Positives = 18/25 (72%)
 Frame = +1

Query: 517 AGTISGLNVLRIINEPTAAAIAYGL 591
           A  + GLNVL+I+N  TA A+ YGL
Sbjct: 127 AAELVGLNVLQIMNSNTAVALNYGL 151



 Score = 30.3 bits (65), Expect = 1.3
 Identities = 12/42 (28%), Positives = 22/42 (52%)
 Frame = +2

Query: 383 PRGSQSMVLTKMXETAEAYLGKTVQNAVITVPAYFNDSQRQA 508
           P     M+L       E +    +++ V+TVP +FN ++R+A
Sbjct: 82  PEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRA 123


>SB_56988| Best HMM Match : DUF229 (HMM E-Value=2e-06)
          Length = 286

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
 Frame = +3

Query: 438 ISAKLCRM-QLSRFPRTSMTLKDKPKR-CRYHLRLERSPNH 554
           +SAKLC +  LS+  R   T     KR C Y +++E SP H
Sbjct: 198 LSAKLCHVVTLSKIVRALQTHSHLHKRQCSYQIQIETSPAH 238


>SB_4173| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 743

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
 Frame = +3

Query: 438 ISAKLCRM-QLSRFPRTSMTLKDKPKR-CRYHLRLERSPNH 554
           +SAKLC +  LS+  R   T     KR C Y +++E SP H
Sbjct: 655 LSAKLCHVITLSKIVRALQTHSHLHKRQCSYQIQIETSPAH 695


>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
          Length = 350

 Score = 29.5 bits (63), Expect = 2.3
 Identities = 16/33 (48%), Positives = 19/33 (57%)
 Frame = -1

Query: 226 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 128
           GS PPG W   R +AR   T  +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236


>SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37)
          Length = 420

 Score = 29.1 bits (62), Expect = 3.1
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = -1

Query: 244 RILCCWGSSPPGSWRH 197
           R++CC G SP   WRH
Sbjct: 395 RVVCCQGDSPSPRWRH 410


>SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9)
          Length = 340

 Score = 29.1 bits (62), Expect = 3.1
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = -1

Query: 244 RILCCWGSSPPGSWRH 197
           R++CC G SP   WRH
Sbjct: 170 RVVCCQGDSPSPRWRH 185


>SB_53143| Best HMM Match : PKD (HMM E-Value=2.7e-18)
          Length = 2111

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 16/64 (25%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
 Frame = +1

Query: 106 HGKVEIIAN-DQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNTIFDAKRSSDVSXKML 282
           H +VE+ A+ D G+R T  +  F D   L  + +++  A+N +N  +  + +     + +
Sbjct: 587 HSEVEMFASADTGSRVT-MFWDFLDGNNLTQELSRDSFALNGHNVTYIYRSNGTFLVEAM 645

Query: 283 LCNP 294
             NP
Sbjct: 646 ASNP 649


>SB_30440| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 123

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 13/50 (26%), Positives = 24/50 (48%)
 Frame = -1

Query: 460 ILHSFAEISFGSFXHFRKHHGLTSSGKKVLSSPLYATLILGLPPSLTTSK 311
           I ++  ++  G     +KHHG   + +K    P+    IL + P L+ S+
Sbjct: 3   IHYNSTQLENGEIKSEKKHHGFIGTFEKRPPDPILQAAILKIEPCLSVSR 52


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,000,274
Number of Sequences: 59808
Number of extensions: 467909
Number of successful extensions: 1263
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1261
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1548368000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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