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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021189
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g12980.1 68414.m01507 AP2 domain-containing transcription fac...    31   0.88 
At2g38970.1 68415.m04789 zinc finger (C3HC4-type RING finger) fa...    29   3.6  
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P...    28   4.7  
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P...    28   4.7  
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF...    28   4.7  
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF...    28   4.7  
At1g15670.1 68414.m01881 kelch repeat-containing F-box family pr...    28   4.7  
At5g44470.1 68418.m05449 hypothetical protein                          28   6.2  
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id...    28   6.2  
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id...    28   6.2  
At4g01810.1 68417.m00238 protein transport protein-related relat...    27   8.2  
At3g46740.1 68416.m05074 chloroplast outer envelope protein, put...    27   8.2  

>At1g12980.1 68414.m01507 AP2 domain-containing transcription
           factor, putative / enhancer of shoot regeneration (ESR1)
           similar to gb|D38124 EREBP-3 from Nicotiana tabacum and
           contains PF|00847 AP2 domain; identical to cDNA enhancer
           of shoot regeneration ESR1 GI:18028939, enhancer of
           shoot regeneration ESR1 [Arabidopsis thaliana]
           GI:18028940
          Length = 328

 Score = 30.7 bits (66), Expect = 0.88
 Identities = 22/72 (30%), Positives = 31/72 (43%), Gaps = 2/72 (2%)
 Frame = -2

Query: 286 PSAAAPFPCVPLSRLSIQFI*RSASIRTVDSDSRMLACLKCRVRLTTILES--GGDSRSY 113
           PSA  P P +PL   +  F    A+ R  ++ S  L    C  R TT   +   G S SY
Sbjct: 134 PSARCPLPSLPLDSSTQNFYGAPAAQRIYNTQSIFLRDASCSSRKTTPYNNSFNGSSSSY 193

Query: 112 VARSESIMRDSD 77
            A   + +  S+
Sbjct: 194 SASKTACVSYSE 205


>At2g38970.1 68415.m04789 zinc finger (C3HC4-type RING finger)
           family protein very low similarity to SP|P97280
           Inter-alpha-trypsin inhibitor heavy chain H3 precursor
           {Mesocricetus auratus}; contains Pfam profiles PF00097:
           Zinc finger, C3HC4 type (RING finger), PF00092: von
           Willebrand factor type A domain
          Length = 692

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = -1

Query: 350 RWLQPRLAVPRQASTSVPRTPAKCCSPIPLRSTQSFVDPIHLKICQYP 207
           RW + +LA+     T +PRT  +  SP PL ST+   D   L    +P
Sbjct: 4   RWRRMKLALGLNLCTYLPRTLEE--SPTPLNSTERLSDVALLSPLNWP 49


>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
           (PRCFC) identical to 20S proteasome beta subunit PBB2
           [Arabidopsis thaliana] GI:3421104, cDNA proteasome
           subunit prcfc GI:2511575
          Length = 274

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 16/53 (30%), Positives = 27/53 (50%)
 Frame = +3

Query: 18  LLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 176
           + P +  C   +AA+ E  T    M+ S L  +  ++  DS++V  LTL  +H
Sbjct: 76  MAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVVTALTLLKKH 125


>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
           (PRCFC) identical to 20S proteasome beta subunit PBB2
           [Arabidopsis thaliana] GI:3421104, cDNA proteasome
           subunit prcfc GI:2511575
          Length = 274

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 16/53 (30%), Positives = 27/53 (50%)
 Frame = +3

Query: 18  LLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 176
           + P +  C   +AA+ E  T    M+ S L  +  ++  DS++V  LTL  +H
Sbjct: 76  MAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVVTALTLLKKH 125


>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
           subunit region (PF03178); contains weak WD-40 repeat
           (PF00400); similar to Splicing factor 3B subunit 3
           (SF3b130)/spliceosomal protein/Splicing factor 3B
           subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
           EMBL:HSAJ1443_1
          Length = 1214

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -1

Query: 374 GRGERSPRRWLQPRLAVPRQASTSVPRTPA 285
           GRG RS  R L+P LA+   A + +P  P+
Sbjct: 426 GRGPRSSLRILRPGLAITEMAVSQLPGQPS 455


>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
           subunit region (PF03178); contains weak WD-40 repeat
           (PF00400); similar to Splicing factor 3B subunit 3
           (SF3b130)/spliceosomal protein/Splicing factor 3B
           subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
           EMBL:HSAJ1443_1
          Length = 1214

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -1

Query: 374 GRGERSPRRWLQPRLAVPRQASTSVPRTPA 285
           GRG RS  R L+P LA+   A + +P  P+
Sbjct: 426 GRGPRSSLRILRPGLAITEMAVSQLPGQPS 455


>At1g15670.1 68414.m01881 kelch repeat-containing F-box family
           protein similar to SP|Q9ER30 Kelch-related protein 1
           (Sarcosin) {Rattus norvegicus}; contains Pfam profiles
           PF01344: Kelch motif, PF00646: F-box domain
          Length = 359

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +3

Query: 546 YVRVRSVGCDGSVQCGAEPGPWRSGSS 626
           + R+ SVG D  V CG +P  WR+  S
Sbjct: 110 FCRLVSVGSDLVVLCGLDPVTWRTSDS 136


>At5g44470.1 68418.m05449 hypothetical protein
          Length = 108

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 13/43 (30%), Positives = 22/43 (51%)
 Frame = +3

Query: 183 IRESESTVRILADLQMNWIDKRLSGTQGNGAAALGWCPRNACG 311
           I  +E+  + + D +  WI+   +  QG   + LGW  RN+ G
Sbjct: 2   IEVTEAVEQAVIDTR-EWIENIATSEQGTSGSGLGWIIRNSHG 43


>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
           identical to 20S proteasome beta subunit PBB1 (PBB1)
           GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
           677-692 (1998)); contains Pfam profile: PF00227
           proteasome A-type and B-type;
          Length = 273

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 15/53 (28%), Positives = 27/53 (50%)
 Frame = +3

Query: 18  LLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 176
           + P +  C   +AA+ E  T    M+ S L  +  ++  DS+++  LTL  +H
Sbjct: 76  MAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVITALTLLKKH 125


>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
           identical to 20S proteasome beta subunit PBB1 (PBB1)
           GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
           677-692 (1998)); contains Pfam profile: PF00227
           proteasome A-type and B-type;
          Length = 267

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 15/53 (28%), Positives = 27/53 (50%)
 Frame = +3

Query: 18  LLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 176
           + P +  C   +AA+ E  T    M+ S L  +  ++  DS+++  LTL  +H
Sbjct: 76  MAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVITALTLLKKH 125


>At4g01810.1 68417.m00238 protein transport protein-related related
           to Sec23 protein [Homo sapiens] gi|1296664|emb|CAA65774
          Length = 880

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/37 (37%), Positives = 17/37 (45%)
 Frame = -1

Query: 344 LQPRLAVPRQASTSVPRTPAKCCSPIPLRSTQSFVDP 234
           L+P    P+   T VP +P    SPIP R  Q    P
Sbjct: 18  LEPNRPSPQPDRTPVPHSPPVVASPIPPRFPQPSFRP 54


>At3g46740.1 68416.m05074 chloroplast outer envelope protein,
           putative similar to chloroplastic outer envelope
           membrane protein (OEP75) [Pisum sativum] GI:633607;
           contains Pfam profile PF01103: outer membrane protein,
           OMP85 family
          Length = 818

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
 Frame = -1

Query: 359 SPRRWLQPRLAVPRQASTSVPRTPA-KCCSPIPLRSTQS 246
           S R++L P  +   + ST  PR P+ KC   +P R T++
Sbjct: 25  SRRKFLSPSSSRLPRISTQSPRVPSIKCSKSLPNRDTET 63


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,798,046
Number of Sequences: 28952
Number of extensions: 352373
Number of successful extensions: 1093
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1038
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1093
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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