Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021185
         (765 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri...   111   5e-25
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast...   109   2e-24
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri...   106   2e-23
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta...    99   4e-21
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident...    90   2e-18
At1g10390.1 68414.m01171 nucleoporin family protein contains Pfa...    28   5.9  
At1g61900.2 68414.m06984 expressed protein contains similarity t...    28   7.8  
At1g61900.1 68414.m06983 expressed protein contains similarity t...    28   7.8  

>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
           putative similar to mitochondrial NAD-dependent malate
           dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score =  111 bits (267), Expect = 5e-25
 Identities = 52/89 (58%), Positives = 73/89 (82%)
 Frame = +1

Query: 256 LVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPG 435
           LV+ L+LYDIA  TPGVAAD+ H+NT ++VSG+ G ++L  A++ AD+V+IPAGVPRKPG
Sbjct: 54  LVSSLSLYDIAN-TPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPG 112

Query: 436 MNRDDLFNTNASIVRDIALSIAQNDPKLL 522
           M RDDLFN NA IV++++++IA+  P+ L
Sbjct: 113 MTRDDLFNINAGIVKNLSIAIAKYCPQAL 141



 Score = 66.5 bits (155), Expect = 2e-11
 Identities = 29/43 (67%), Positives = 37/43 (86%)
 Frame = +3

Query: 510 PKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDVV 638
           P+A+V +I+NPVNSTVPIA+E+ KKAG YD  ++ GVTTLDVV
Sbjct: 138 PQALVNMISNPVNSTVPIAAEIFKKAGTYDEKKLFGVTTLDVV 180



 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 18/36 (50%), Positives = 24/36 (66%)
 Frame = +2

Query: 647 TFVGEINGVDPTSVAVPVIGGHSGITIIPILSQCQP 754
           TF    + V+   V VPV+GGH+GITI+P+ SQ  P
Sbjct: 184 TFYAGKSDVNVAEVNVPVVGGHAGITILPLFSQASP 219


>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
           (MDH) identical to chloroplast NAD-malate dehydrogenase
           [Arabidopsis thaliana] GI:3256066; contains InterPro
           entry IPR001236: Lactate/malate dehydrogenase; contains
           Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
           binding domain  and PF02866: lactate/malate
           dehydrogenase, alpha/beta C-terminal domain
          Length = 403

 Score =  109 bits (262), Expect = 2e-24
 Identities = 54/86 (62%), Positives = 66/86 (76%)
 Frame = +1

Query: 256 LVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPG 435
           LV+ L LYDIA V  GVAADLSH NTP++V    GP EL+  +KD +VVVIPAGVPRKPG
Sbjct: 107 LVSTLHLYDIANVK-GVAADLSHCNTPSQVRDFTGPSELADCLKDVNVVVIPAGVPRKPG 165

Query: 436 MNRDDLFNTNASIVRDIALSIAQNDP 513
           M RDDLFN NA+IV+ +  ++A+N P
Sbjct: 166 MTRDDLFNINANIVKTLVEAVAENCP 191



 Score = 70.9 bits (166), Expect = 8e-13
 Identities = 32/43 (74%), Positives = 37/43 (86%)
 Frame = +3

Query: 510 PKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDVV 638
           P A + II+NPVNSTVPIA+EVLKK GVYDP ++ GVTTLDVV
Sbjct: 191 PNAFIHIISNPVNSTVPIAAEVLKKKGVYDPKKLFGVTTLDVV 233



 Score = 44.4 bits (100), Expect = 8e-05
 Identities = 19/38 (50%), Positives = 28/38 (73%)
 Frame = +2

Query: 647 TFVGEINGVDPTSVAVPVIGGHSGITIIPILSQCQPAL 760
           TFV +   +    V VPVIGGH+GITI+P+LS+ +P++
Sbjct: 237 TFVSQKKNLKLIDVDVPVIGGHAGITILPLLSKTKPSV 274


>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
           identical to mitochondrial NAD-dependent malate
           dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score =  106 bits (254), Expect = 2e-23
 Identities = 51/89 (57%), Positives = 69/89 (77%)
 Frame = +1

Query: 256 LVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPG 435
           LV+ L+LYDIA  TPGVAAD+ H+NT ++V G+ G + L+ A++ AD+V+IPAGVPRKPG
Sbjct: 54  LVSSLSLYDIAN-TPGVAADVGHINTRSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPG 112

Query: 436 MNRDDLFNTNASIVRDIALSIAQNDPKLL 522
           M RDDLFN NA IV+++  +IA+  P  L
Sbjct: 113 MTRDDLFNINAGIVKNLCTAIAKYCPHAL 141



 Score = 65.7 bits (153), Expect = 3e-11
 Identities = 28/43 (65%), Positives = 37/43 (86%)
 Frame = +3

Query: 510 PKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDVV 638
           P A++ +I+NPVNSTVPIA+E+ KKAG+YD  ++ GVTTLDVV
Sbjct: 138 PHALINMISNPVNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVV 180



 Score = 40.7 bits (91), Expect = 0.001
 Identities = 18/35 (51%), Positives = 24/35 (68%)
 Frame = +2

Query: 650 FVGEINGVDPTSVAVPVIGGHSGITIIPILSQCQP 754
           + G+ N V    V VPVIGGH+G+TI+P+ SQ  P
Sbjct: 186 YAGKAN-VPVAEVNVPVIGGHAGVTILPLFSQATP 219


>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
           putative strong similarity to glyoxysomal malate
           dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
           lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
           GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
           {Arabidopsis thaliana}, SP|P37228 {Glycine max};
           contains InterPro entry IPR001236: Lactate/malate
           dehydrogenase
          Length = 354

 Score = 98.7 bits (235), Expect = 4e-21
 Identities = 50/89 (56%), Positives = 63/89 (70%)
 Frame = +1

Query: 256 LVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPG 435
           LV+ L LYD+A   PGV AD+SHM+T A V G  G  +L  A+   D+V+IPAGVPRKPG
Sbjct: 67  LVSVLHLYDVANA-PGVTADISHMDTSAVVRGFLGQPQLEEALTGMDLVIIPAGVPRKPG 125

Query: 436 MNRDDLFNTNASIVRDIALSIAQNDPKLL 522
           M RDDLFN NA IVR ++ +IA+  PK +
Sbjct: 126 MTRDDLFNINAGIVRTLSEAIAKCCPKAI 154



 Score = 70.5 bits (165), Expect = 1e-12
 Identities = 32/43 (74%), Positives = 38/43 (88%)
 Frame = +3

Query: 510 PKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDVV 638
           PKAIV II+NPVNSTVPIA+EV KKAG +DP +++GVT LDVV
Sbjct: 151 PKAIVNIISNPVNSTVPIAAEVFKKAGTFDPKKLMGVTMLDVV 193



 Score = 54.4 bits (125), Expect = 8e-08
 Identities = 21/36 (58%), Positives = 29/36 (80%)
 Frame = +2

Query: 647 TFVGEINGVDPTSVAVPVIGGHSGITIIPILSQCQP 754
           TFV E+  +DP  V VPV+GGH+G+TI+P+LSQ +P
Sbjct: 197 TFVAEVMSLDPREVEVPVVGGHAGVTILPLLSQVKP 232


>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
           identical to SP|Q9ZP05; identical to cDNA microbody
           NAD-dependent malate dehydrogenase GI:3929650
          Length = 354

 Score = 89.8 bits (213), Expect = 2e-18
 Identities = 44/89 (49%), Positives = 59/89 (66%)
 Frame = +1

Query: 256 LVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPG 435
           LV+ L LYD+    PGV AD+SHM+T A V G  G ++L  A+   D+V+IPAG+PRKPG
Sbjct: 67  LVSLLHLYDVVNA-PGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPAGIPRKPG 125

Query: 436 MNRDDLFNTNASIVRDIALSIAQNDPKLL 522
           M RDDLF  NA IV+ +   +A+  P  +
Sbjct: 126 MTRDDLFKINAGIVKTLCEGVAKCCPNAI 154



 Score = 70.9 bits (166), Expect = 8e-13
 Identities = 32/42 (76%), Positives = 37/42 (88%)
 Frame = +3

Query: 510 PKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDV 635
           P AIV +I+NPVNSTVPIA+EV KKAG YDP ++LGVTTLDV
Sbjct: 151 PNAIVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTTLDV 192



 Score = 56.0 bits (129), Expect = 3e-08
 Identities = 22/36 (61%), Positives = 30/36 (83%)
 Frame = +2

Query: 647 TFVGEINGVDPTSVAVPVIGGHSGITIIPILSQCQP 754
           TFV E+ G+DP  V VPV+GGH+G+TI+P+LSQ +P
Sbjct: 197 TFVAEVLGLDPREVDVPVVGGHAGVTILPLLSQVKP 232


>At1g10390.1 68414.m01171 nucleoporin family protein contains Pfam
           profiles: PF04096 nucleoporin autopeptidase, PF03093
           nucleoporin FG repeat family
          Length = 1041

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 21/53 (39%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
 Frame = -3

Query: 646 GSATTSRVVTPSTRFG-SYTPAFLSTSEAIGTVELTGFVMMATIALGHSEQWT 491
           GS++     TPS  FG S TP F S+S   G+    G    AT A G+S+  T
Sbjct: 492 GSSSAFGTTTPSPLFGSSSTPGFGSSSSIFGSAPGQG----ATPAFGNSQPST 540


>At1g61900.2 68414.m06984 expressed protein contains similarity to
           glutamic acid/alanine-rich protein GI:6707830 from
           [Trypanosoma congolense]
          Length = 413

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = -2

Query: 608 AVRVVHSRLLEHFRGNRHCRVNRVC 534
           A ++  SR+ E  RG  +C++NRVC
Sbjct: 238 ATKLDPSRVKETLRGLANCKINRVC 262


>At1g61900.1 68414.m06983 expressed protein contains similarity to
           glutamic acid/alanine-rich protein GI:6707830 from
           [Trypanosoma congolense]
          Length = 433

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = -2

Query: 608 AVRVVHSRLLEHFRGNRHCRVNRVC 534
           A ++  SR+ E  RG  +C++NRVC
Sbjct: 238 ATKLDPSRVKETLRGLANCKINRVC 262


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,211,690
Number of Sequences: 28952
Number of extensions: 357451
Number of successful extensions: 971
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 928
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 971
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)