Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021183
(825 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 127 2e-30
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 73 4e-14
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 73 7e-14
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 69 1e-12
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 35 0.012
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 33 0.065
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 31 0.15
SPCC830.05c |epl1||histone acetyltransferase complex subunit Epl... 29 1.1
SPBC1271.12 |kes1||oxysterol binding protein |Schizosaccharomyce... 29 1.1
SPCC70.07c |tmp1|tmp|thymidylate kinase Tmp1|Schizosaccharomyces... 27 2.4
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 27 3.2
SPAC20G4.01 ||SPAC22F8.13|CCR4-Not complex subunit Caf16|Schizos... 27 3.2
SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosa... 26 5.6
SPCC306.04c |set1||histone lysine methyltransferase Set1|Schizos... 26 7.5
SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha... 26 7.5
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ... 26 7.5
SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces p... 25 9.9
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 127 bits (306), Expect = 2e-30
Identities = 59/80 (73%), Positives = 66/80 (82%)
Frame = +2
Query: 257 MRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCP 436
+RFTTFDLGGHQQARR+WRDYFP V+ IV+LVD CD RL ESKAELD+LL E L+ P
Sbjct: 64 VRFTTFDLGGHQQARRLWRDYFPEVNGIVYLVDCCDFERLSESKAELDALLAMEELARVP 123
Query: 437 VLILGNKIDKPGAASEDELR 496
LILGNKID PGA SEDEL+
Sbjct: 124 FLILGNKIDAPGAISEDELK 143
Score = 95.9 bits (228), Expect = 6e-21
Identities = 45/62 (72%), Positives = 50/62 (80%)
Frame = +3
Query: 69 MFILDWFTGVLGFLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPTLHPTSEELS 248
MFI++WF L LGL K K+LFLGLDNAGKTTLLHMLK+DRLA PTLHPTSEEL+
Sbjct: 1 MFIINWFYDALAMLGLVNKHAKMLFLGLDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELA 60
Query: 249 IG 254
IG
Sbjct: 61 IG 62
Score = 64.9 bits (151), Expect = 1e-11
Identities = 31/43 (72%), Positives = 38/43 (88%), Gaps = 1/43 (2%)
Frame = +1
Query: 517 QTTGKGKVSRSELPG-RPLELFMCSVLKRQGYGEGFRWLAQYI 642
QTTGKG VS+ +PG RP+E+FMCSV+ RQGYGEGF+WLAQY+
Sbjct: 150 QTTGKG-VSKP-VPGIRPIEVFMCSVVLRQGYGEGFKWLAQYV 190
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 73.3 bits (172), Expect = 4e-14
Identities = 33/84 (39%), Positives = 51/84 (60%)
Frame = +2
Query: 257 MRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCP 436
+RFT +D+GG + R W++YF + +AI+++VD+ D RL E + L LL +E L
Sbjct: 60 LRFTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDLRLEECRNTLQELLVEEKLLFTS 119
Query: 437 VLILGNKIDKPGAASEDELRHFFN 508
+L+L NK D GA S +E+ N
Sbjct: 120 ILVLANKSDVSGALSSEEISKILN 143
Score = 33.9 bits (74), Expect = 0.028
Identities = 16/36 (44%), Positives = 25/36 (69%)
Frame = +3
Query: 114 LWKKSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPT 221
L ++ ++L LGLDNAGKTT+L L ++ + + PT
Sbjct: 12 LKEREVRVLLLGLDNAGKTTILKCLLNEDVNEVSPT 47
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 72.5 bits (170), Expect = 7e-14
Identities = 28/81 (34%), Positives = 51/81 (62%)
Frame = +2
Query: 251 RRMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSN 430
+ ++F +D+GG + R +WR YF ++F+VD+ D R+ E++ EL +++D + +
Sbjct: 63 KNIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDSADSNRISEARQELHRIISDREMRD 122
Query: 431 CPVLILGNKIDKPGAASEDEL 493
C +L+L NK D PGA S ++
Sbjct: 123 CLLLVLANKQDLPGALSPAQI 143
Score = 35.1 bits (77), Expect = 0.012
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 123 KSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPTL 224
K ++L LGLD AGKTT+L+ LK ++ +PT+
Sbjct: 20 KEMRILMLGLDAAGKTTILYKLKLNQSVVTIPTV 53
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 68.5 bits (160), Expect = 1e-12
Identities = 30/81 (37%), Positives = 48/81 (59%)
Frame = +2
Query: 251 RRMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSN 430
R + FT +D+GG + R +WR YF I+F+VD+ DR R+ E+ EL +L ++ L +
Sbjct: 59 RNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRERISEAHEELQRMLNEDELRD 118
Query: 431 CPVLILGNKIDKPGAASEDEL 493
+L+ NK D P A + E+
Sbjct: 119 ALLLVFANKQDLPNAMNAAEI 139
Score = 35.9 bits (79), Expect = 0.007
Identities = 15/35 (42%), Positives = 24/35 (68%)
Frame = +3
Query: 120 KKSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPTL 224
K+ ++L +GLD AGKTT+L+ LK + +PT+
Sbjct: 15 KREMRILMVGLDAAGKTTILYKLKLGEIVTTIPTI 49
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 35.1 bits (77), Expect = 0.012
Identities = 23/81 (28%), Positives = 39/81 (48%), Gaps = 12/81 (14%)
Frame = +2
Query: 257 MRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVD------------ACDRPRLPESKAELD 400
++ FD+GG + RR W F V++I+F V A +R RL ES + D
Sbjct: 192 LQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYDKKLYERAAPERNRLVESISLFD 251
Query: 401 SLLTDETLSNCPVLILGNKID 463
S++ + + +++ NK D
Sbjct: 252 SIINSQWFMHSSIILFLNKFD 272
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 32.7 bits (71), Expect = 0.065
Identities = 23/80 (28%), Positives = 36/80 (45%), Gaps = 10/80 (12%)
Frame = +2
Query: 254 RMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLV-----DAC-----DRPRLPESKAELDS 403
R + FD+GG + RR W F V A++FLV D C ++ E+ DS
Sbjct: 233 RHHYRFFDVGGQRSERRKWIHCFENVTALLFLVSLAGYDQCLVEDNSGNQMQEALLLWDS 292
Query: 404 LLTDETLSNCPVLILGNKID 463
+ S +++ NK+D
Sbjct: 293 ICNSSWFSESAMILFLNKLD 312
>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2493
Score = 31.5 bits (68), Expect = 0.15
Identities = 24/78 (30%), Positives = 38/78 (48%), Gaps = 4/78 (5%)
Frame = +2
Query: 353 DACDRPRLPESKAELDSLLTDETLSNCPVL--ILGNKIDKPGAASEDELRHFFNCISRPL 526
D+ +P LP+ + SL++DE+ + +L +L N + P + L HF CI
Sbjct: 401 DSFIKPSLPKF---ISSLVSDESQKSLAILESVLDNSVPVPVDVASVSLNHFVYCI---- 453
Query: 527 EREKYQGPSYP--VGRWS 574
ER + PSY + WS
Sbjct: 454 ERLQIVSPSYEDIISLWS 471
>SPCC830.05c |epl1||histone acetyltransferase complex subunit Epl1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 28.7 bits (61), Expect = 1.1
Identities = 21/52 (40%), Positives = 26/52 (50%)
Frame = -3
Query: 256 TPIDSSSDVGCKVGTC*ASLSSFNMCKSVVFPALSSPRNKSLPDFFHRPRNP 101
TP SD G T +SLS+FN C S+ F + +S S H PRNP
Sbjct: 435 TPSTPLSDNGPTYSTPHSSLSNFNTCDSLSFSSNNSLYGYS--TLLH-PRNP 483
>SPBC1271.12 |kes1||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 388
Score = 28.7 bits (61), Expect = 1.1
Identities = 11/38 (28%), Positives = 24/38 (63%)
Frame = +2
Query: 284 GHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAEL 397
G ++RRVW+++ A+DA + + ++ ++ E + EL
Sbjct: 306 GEYESRRVWKNFAVALDAGDYAAASQEKSKIEEGQREL 343
>SPCC70.07c |tmp1|tmp|thymidylate kinase Tmp1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 210
Score = 27.5 bits (58), Expect = 2.4
Identities = 12/29 (41%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
Frame = +3
Query: 129 GKLLFL-GLDNAGKTTLLHMLKDDRLAQH 212
G+L+ + GLD +GK+T +L D ++QH
Sbjct: 7 GRLIVIEGLDRSGKSTQCQLLVDKLISQH 35
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 27.1 bits (57), Expect = 3.2
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +1
Query: 61 IQKCLSSTGSLECWDFLVYGKSQASSCFW 147
I++ L + GSL D L+ G S A FW
Sbjct: 658 IKRVLENVGSLNHEDILLIGSSNAKYSFW 686
>SPAC20G4.01 ||SPAC22F8.13|CCR4-Not complex subunit
Caf16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 280
Score = 27.1 bits (57), Expect = 3.2
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 123 KSGKLLFLGLDNAGKTTLLHMLKDDRLAQ 209
K + L +G + AGK+TLL +L LA+
Sbjct: 29 KGSRTLLVGANGAGKSTLLKLLSGKSLAK 57
>SPBC1289.12 |usp109||U1 snRNP-associated protein
Usp109|Schizosaccharomyces pombe|chr 2|||Manual
Length = 352
Score = 26.2 bits (55), Expect = 5.6
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -3
Query: 178 KSVVFPALSSPRNKSLPDFFHRPRNPNTPVN 86
+S +PAL++P S+ D + P P +P+N
Sbjct: 256 QSTYWPALAAPVYPSMKDVPNNPFTPFSPIN 286
>SPCC306.04c |set1||histone lysine methyltransferase
Set1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 920
Score = 25.8 bits (54), Expect = 7.5
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +2
Query: 386 KAELDSLLTDETLSNCPVLILGNKIDKPGAASEDEL 493
K E+D + ET S+ +L I K G+A +DEL
Sbjct: 556 KMEVDYTSSSETESDASILSPSAAIPKSGSAIKDEL 591
>SPBC660.15 |||mRNA cleavage factor complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 474
Score = 25.8 bits (54), Expect = 7.5
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 467 PGAASEDELRHFFNCISRPLE 529
PG +E+E R+FFN R L+
Sbjct: 255 PGDCTEEEFRNFFNQFGRVLD 275
>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 2|||Manual
Length = 827
Score = 25.8 bits (54), Expect = 7.5
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -3
Query: 160 ALSSPRNKSLPDFFHRPRNPNTPVNQS 80
A S N LP F P + ++PVNQS
Sbjct: 73 AEESDPNLKLPSFLAPPNDKDSPVNQS 99
>SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 200
Score = 25.4 bits (53), Expect = 9.9
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 3/74 (4%)
Frame = +2
Query: 251 RRMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETL-- 424
R + +D G + R+ +P D+ V L+ C PES + E L
Sbjct: 52 RHIELALWDTAGQEDYDRLRPLSYP--DSHVVLI--CFSVDAPESLDNVQEKWISEVLHF 107
Query: 425 -SNCPVLILGNKID 463
SN P+L++G K+D
Sbjct: 108 CSNLPILLVGCKVD 121
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,165,603
Number of Sequences: 5004
Number of extensions: 63147
Number of successful extensions: 228
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 215
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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