Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021181
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g05360.2 68418.m00577 expressed protein similar to unknown pr... 33 0.18
At5g05360.1 68418.m00578 expressed protein similar to unknown pr... 33 0.18
At5g51460.3 68418.m06381 trehalose-6-phosphate phosphatase (TPPA... 31 0.54
At5g51460.2 68418.m06380 trehalose-6-phosphate phosphatase (TPPA... 31 0.54
At5g51460.1 68418.m06379 trehalose-6-phosphate phosphatase (TPPA... 31 0.54
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 31 0.71
At3g50060.1 68416.m05473 myb family transcription factor contain... 31 0.71
At2g25730.1 68415.m03084 expressed protein 30 1.2
At3g52160.1 68416.m05726 beta-ketoacyl-CoA synthase family prote... 29 2.9
At1g07540.1 68414.m00807 telomere-binding protein, putative simi... 28 5.0
At4g33590.1 68417.m04772 hypothetical protein 28 6.6
At4g05190.1 68417.m00781 kinesin-like protein A, putative kinesi... 28 6.6
At3g62370.1 68416.m07006 expressed protein 28 6.6
At1g67340.1 68414.m07665 zinc finger (MYND type) family protein ... 28 6.6
At3g47910.1 68416.m05224 expressed protein low similarity to non... 27 8.8
At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /... 27 8.8
>At5g05360.2 68418.m00577 expressed protein similar to unknown
protein (pir||T02500)
Length = 153
Score = 33.1 bits (72), Expect = 0.18
Identities = 24/68 (35%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = -1
Query: 375 PLPPNRVSNETMKVVVFQRRSRETISHLCYTSHVSLQCQTKSSSTGSSFPADSPKP---V 205
P P+R S V ++ +R+T SHL Y++ V L+ + S S + PA P V
Sbjct: 20 PTRPHRPSPSPRNKVFVKKTTRDTTSHLDYSNLVKLE-KAGSHSGSNPAPASGSDPINRV 78
Query: 204 PLAVVSLD 181
PLA V D
Sbjct: 79 PLAQVVED 86
>At5g05360.1 68418.m00578 expressed protein similar to unknown
protein (pir||T02500)
Length = 163
Score = 33.1 bits (72), Expect = 0.18
Identities = 24/68 (35%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = -1
Query: 375 PLPPNRVSNETMKVVVFQRRSRETISHLCYTSHVSLQCQTKSSSTGSSFPADSPKP---V 205
P P+R S V ++ +R+T SHL Y++ V L+ + S S + PA P V
Sbjct: 20 PTRPHRPSPSPRNKVFVKKTTRDTTSHLDYSNLVKLE-KAGSHSGSNPAPASGSDPINRV 78
Query: 204 PLAVVSLD 181
PLA V D
Sbjct: 79 PLAQVVED 86
>At5g51460.3 68418.m06381 trehalose-6-phosphate phosphatase (TPPA)
identical to trehalose-6-phosphate phosphatase (AtTPPA)
[Arabidopsis thaliana] GI:2944178
Length = 385
Score = 31.5 bits (68), Expect = 0.54
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = -1
Query: 561 ELALGHLRYSLTDVPPQSTPRLAVSSNRITREF*TATSVSATSPLCTLGTKHRAPADIID 382
++ GH +TD PP S RL + NR+ AT++S + L L T R I+D
Sbjct: 2 DMKSGHSSPVMTDSPPISNSRLTIRQNRLPYSSAAATAISQNNNL--LLTVPRKKTGILD 59
>At5g51460.2 68418.m06380 trehalose-6-phosphate phosphatase (TPPA)
identical to trehalose-6-phosphate phosphatase (AtTPPA)
[Arabidopsis thaliana] GI:2944178
Length = 384
Score = 31.5 bits (68), Expect = 0.54
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = -1
Query: 561 ELALGHLRYSLTDVPPQSTPRLAVSSNRITREF*TATSVSATSPLCTLGTKHRAPADIID 382
++ GH +TD PP S RL + NR+ AT++S + L L T R I+D
Sbjct: 2 DMKSGHSSPVMTDSPPISNSRLTIRQNRLPYSSAAATAISQNNNL--LLTVPRKKTGILD 59
>At5g51460.1 68418.m06379 trehalose-6-phosphate phosphatase (TPPA)
identical to trehalose-6-phosphate phosphatase (AtTPPA)
[Arabidopsis thaliana] GI:2944178
Length = 385
Score = 31.5 bits (68), Expect = 0.54
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = -1
Query: 561 ELALGHLRYSLTDVPPQSTPRLAVSSNRITREF*TATSVSATSPLCTLGTKHRAPADIID 382
++ GH +TD PP S RL + NR+ AT++S + L L T R I+D
Sbjct: 2 DMKSGHSSPVMTDSPPISNSRLTIRQNRLPYSSAAATAISQNNNL--LLTVPRKKTGILD 59
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 31.1 bits (67), Expect = 0.71
Identities = 17/49 (34%), Positives = 27/49 (55%)
Frame = -1
Query: 420 LGTKHRAPADIIDRAPLPPNRVSNETMKVVVFQRRSRETISHLCYTSHV 274
LG +H PA +I+R P PP +S +++++F E +CY S V
Sbjct: 231 LGLEHVLPA-VIERIPPPPG-ISESPLRMLLFDSFFNEYKGVICYVSVV 277
>At3g50060.1 68416.m05473 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain;
identical to cDNA MYB-related protein (1107 bp)
GI:1263096
Length = 301
Score = 31.1 bits (67), Expect = 0.71
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = +3
Query: 450 SSPFKTPA*SGSRTLPGGELTGAVHLSKNNAGVLRPAQRGQKPRVEQKGK 599
SS + P S S +LPG E T + H + NNA + + K VE++G+
Sbjct: 195 SSSSEDPPTSLSLSLPGAENTSSSHNNNNNALMFPRFESQMKINVEERGE 244
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 30.3 bits (65), Expect = 1.2
Identities = 29/112 (25%), Positives = 47/112 (41%), Gaps = 1/112 (0%)
Frame = -1
Query: 534 SLTDVPPQSTPRLAVSSNRITREF*TATSVSATSPLCTLGTKHRAPADIIDRAPLPPNRV 355
S+T +PP PR+ VS++R+ ++ +V A S +L R A P
Sbjct: 1626 SVTIIPPPREPRITVSASRLRQKSRAGPAVKA-SFTSSLSNFQREARRAFSWA---PRNA 1681
Query: 354 SNETMKVVVFQRRSRETISHLCYTSHVSLQCQTK-SSSTGSSFPADSPKPVP 202
N T V+++R S L + + + T GSS+ AD +P
Sbjct: 1682 ENRTTSKDVYRKRKN---SGLGASERAAWEAMTGIQEDQGSSYSADGQDRLP 1730
>At3g52160.1 68416.m05726 beta-ketoacyl-CoA synthase family protein
beta-ketoacyl-CoA synthase - Simmondsia
chinensis,PID:g1045614
Length = 451
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +1
Query: 559 LSEDRNLAWSKRAKSGLIQMFSTHRDCESTAYRSFSIK 672
LS R W RAK L+Q+ TH+ E T+Y+S ++
Sbjct: 282 LSSRRIDRW--RAKYQLMQLVRTHKGMEDTSYKSIELR 317
>At1g07540.1 68414.m00807 telomere-binding protein, putative similar
to telomere binding protein TBP1 [Nicotiana glutinosa]
gi|23664357|gb|AAN39330
Length = 622
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = -1
Query: 534 SLTDVPPQSTPRLAVSSNRITREF*TATSVSATSPLCTLGT 412
+LT VPP+ T R V+ RI R F A + + LGT
Sbjct: 500 ALTVVPPRKTKRSEVAQRRIRRPFSVAEVEALVQAVERLGT 540
>At4g33590.1 68417.m04772 hypothetical protein
Length = 466
Score = 27.9 bits (59), Expect = 6.6
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = -1
Query: 261 QTKSSSTGSSFPADSPKPVPLAVVSLDSR*GQWES 157
QTK + FP+DS K L + +D G W S
Sbjct: 109 QTKGEAQYQEFPSDSSKGRLLCLKGVDEHDGSWNS 143
>At4g05190.1 68417.m00781 kinesin-like protein A, putative kinesin
like protein A, Arabidopsis thaliana, gb:Q07970
Length = 790
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -1
Query: 384 DRAPLPPNRVSNETMKVVVFQRRSRET 304
+RAPLP V E + + F +R +ET
Sbjct: 7 NRAPLPSPNVKKEALSSIPFDKRRKET 33
>At3g62370.1 68416.m07006 expressed protein
Length = 361
Score = 27.9 bits (59), Expect = 6.6
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -3
Query: 508 NSPPGSVL--EPDHAGVLNGDERFRHVTTLHAWN 413
N+ PG + P G NG +RF H+ ++AWN
Sbjct: 160 NAIPGRLYGGNPIDNGEGNGGDRFGHLVDIYAWN 193
>At1g67340.1 68414.m07665 zinc finger (MYND type) family protein /
F-box family protein
Length = 379
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = -1
Query: 435 SPLCTLGTKHRAPADIIDRAPLPPNRVSNETMKVVVFQRRSRETIS 298
S LC LG+ R PAD I+ L R+ M +V R S + I+
Sbjct: 54 SILCKLGSTSRCPADFIN-VLLTCKRLKGLAMNPIVLSRLSPKAIA 98
>At3g47910.1 68416.m05224 expressed protein low similarity to
nonmuscle myosin heavy chain (NMHC) [Homo sapiens]
GI:189036; contains Pfam profiles PF04780: Protein of
unknown function (DUF629), PF04781: Protein of unknown
function (DUF627)
Length = 1290
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -1
Query: 423 TLGTKHRAPADIIDRAPLPPNRVSNETMKVVV 328
T+G+ HR+ AD ++ +PLP V + + +V
Sbjct: 972 TIGSDHRSNADSVEHSPLPVAPVGDHSEADIV 1003
>At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /
CAF identical to RNA helicase/RNAseIII CAF protein
GB:AAF03534 GI:6102610 from [Arabidopsis thaliana]
Length = 1909
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/44 (29%), Positives = 22/44 (50%)
Frame = +3
Query: 105 GSQMPRHLISDAHEWINEIPTVPIYYLAKPQPRERAWENQRGKK 236
G +P + +SD + INE+ P +AK +E + GK+
Sbjct: 70 GGGLPNNGVSDTNSQINEVTVTP-QVIAKETVKENGLQKNGGKR 112
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,462,971
Number of Sequences: 28952
Number of extensions: 333979
Number of successful extensions: 885
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 885
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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