Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021167
(718 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 126 2e-30
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 73 3e-14
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 73 3e-14
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 55 1e-08
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 51 1e-07
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 39 6e-04
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 39 6e-04
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 33 0.041
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 31 0.22
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.66
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 27 2.7
SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosacchar... 27 3.5
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 26 6.2
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 26 6.2
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 6.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 126 bits (305), Expect = 2e-30
Identities = 59/70 (84%), Positives = 61/70 (87%)
Frame = -2
Query: 717 ASSSSLEKSYELPDGQVITIGNERFRCPEALFHPSFLGMEACGIHETTYNSIMKCDVDIR 538
A SSSLEKSYELPDGQVITIGNERFR PEALF PS LG+E GIHE TYNSIMKCDVDIR
Sbjct: 231 AQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIR 290
Query: 537 KDLYANTVLS 508
KDLY N V+S
Sbjct: 291 KDLYGNVVMS 300
Score = 111 bits (267), Expect = 1e-25
Identities = 51/55 (92%), Positives = 54/55 (98%)
Frame = -1
Query: 508 GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQM 344
GGTTMYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQM
Sbjct: 301 GGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQM 355
Score = 41.5 bits (93), Expect = 1e-04
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = -3
Query: 341 ISKQEYDESGPSIVHRKCF 285
ISKQEYDESGP IV+RKCF
Sbjct: 357 ISKQEYDESGPGIVYRKCF 375
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 73.3 bits (172), Expect = 3e-14
Identities = 31/62 (50%), Positives = 42/62 (67%)
Frame = -2
Query: 693 SYELPDGQVITIGNERFRCPEALFHPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTV 514
+Y+LPD Q IT+G E F PE LF+P +G EA G+H + SI+ D+D+R LY+N V
Sbjct: 244 TYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIV 303
Query: 513 LS 508
LS
Sbjct: 304 LS 305
Score = 59.3 bits (137), Expect = 5e-10
Identities = 25/55 (45%), Positives = 39/55 (70%)
Frame = -1
Query: 508 GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQM 344
GG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++
Sbjct: 306 GGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRL 360
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 73.3 bits (172), Expect = 3e-14
Identities = 33/65 (50%), Positives = 44/65 (67%)
Frame = -2
Query: 702 LEKSYELPDGQVITIGNERFRCPEALFHPSFLGMEACGIHETTYNSIMKCDVDIRKDLYA 523
L ++Y LPDG+VI +G+ER+ CPE LF P +G E G+ E +++I DVDIRK LY
Sbjct: 236 LMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYR 295
Query: 522 NTVLS 508
VLS
Sbjct: 296 AIVLS 300
Score = 39.5 bits (88), Expect = 5e-04
Identities = 24/68 (35%), Positives = 40/68 (58%), Gaps = 11/68 (16%)
Frame = -1
Query: 532 LVRQHRIVGGTTMYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWI 386
L R + GG++MY G+ R++KEI L P+ + K+KI P R+++V+I
Sbjct: 293 LYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFI 352
Query: 385 GGSILASL 362
GG++LA +
Sbjct: 353 GGAVLADI 360
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 54.8 bits (126), Expect = 1e-08
Identities = 30/78 (38%), Positives = 40/78 (51%), Gaps = 9/78 (11%)
Frame = -2
Query: 714 SSSSLEKSYELPDGQVITIGNERFRCPEALFHPSFLGMEAC---------GIHETTYNSI 562
+SS K +E PDG G ERFR E LF+PSF + G+HE Y SI
Sbjct: 273 ASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSI 332
Query: 561 MKCDVDIRKDLYANTVLS 508
+ CD ++R L N V++
Sbjct: 333 LACDSELRSPLLNNIVVT 350
Score = 46.4 bits (105), Expect = 4e-06
Identities = 23/59 (38%), Positives = 35/59 (59%), Gaps = 2/59 (3%)
Frame = -1
Query: 514 IVGGTTMYPGIADRMQKEITALAP-STMKIKII-APPERKYSVWIGGSILASLSTFQQM 344
+ GGT++ PG+++R+Q E+ LA S + + +VW GGSILASL FQ +
Sbjct: 349 VTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHL 407
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 51.2 bits (117), Expect = 1e-07
Identities = 26/60 (43%), Positives = 40/60 (66%), Gaps = 3/60 (5%)
Frame = -1
Query: 514 IVGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASLSTFQQM 344
+ GGT++ G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL TF Q+
Sbjct: 352 VCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSLGTFHQL 410
Score = 32.3 bits (70), Expect(2) = 2e-04
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = -2
Query: 699 EKSYELPDGQVITIGNERFRCPEALFHP 616
E +E PDG + G ER++ PE LF P
Sbjct: 261 ESEFEFPDGSRMMFGAERYQIPEHLFVP 288
Score = 27.9 bits (59), Expect(2) = 2e-04
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 591 GIHETTYNSIMKCDVDIRKDLYANTVL 511
GI + N I +CDVDIR L N ++
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIV 352
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 39.1 bits (87), Expect = 6e-04
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = -2
Query: 672 QVITIGNERFRCPEALFHPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTV 514
QV+ + NERF PE LF PS + + GI E S+ +I+ L N V
Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVV 324
Score = 33.9 bits (74), Expect = 0.023
Identities = 11/35 (31%), Positives = 21/35 (60%)
Frame = -1
Query: 511 VGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPE 407
+GG +PG R+ E+ +LAP+ ++K+ P +
Sbjct: 326 IGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSD 360
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 39.1 bits (87), Expect = 6e-04
Identities = 20/54 (37%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = -2
Query: 666 ITIGNERFRCPEALFHPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLS 508
I +G ERF PE F+P + + E N + +D+RK LY N VLS
Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLS 333
Score = 28.7 bits (61), Expect = 0.88
Identities = 16/75 (21%), Positives = 35/75 (46%), Gaps = 14/75 (18%)
Frame = -1
Query: 535 GLVRQHRIVGGTTMYPGIADRMQKEITALAPSTMK--------------IKIIAPPERKY 398
GL + + GG+T++ +R+Q+++ + + + +I+ ++
Sbjct: 325 GLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRN 384
Query: 397 SVWIGGSILASLSTF 353
+VW GGS+LA F
Sbjct: 385 AVWFGGSLLAQTPEF 399
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 33.1 bits (72), Expect = 0.041
Identities = 13/48 (27%), Positives = 29/48 (60%)
Frame = -2
Query: 651 ERFRCPEALFHPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLS 508
E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLIT 641
Score = 31.1 bits (67), Expect = 0.16
Identities = 15/52 (28%), Positives = 23/52 (44%)
Frame = -1
Query: 532 LVRQHRIVGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGS 377
LV I GG PG+ R+++E+T++ P I + W G S
Sbjct: 634 LVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGAS 685
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 30.7 bits (66), Expect = 0.22
Identities = 16/64 (25%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = -1
Query: 514 IVGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASLSTFQQM 344
IVGG +PG A +++ I + + I +I PP + ++ W G I + ++
Sbjct: 535 IVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRIRIVSEL 594
Query: 343 *SRN 332
+N
Sbjct: 595 WIKN 598
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 29.1 bits (62), Expect = 0.66
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = -1
Query: 334 NRSTTSLAPPLYTGSASKRTARRCLQHPRPAAQFRLVISVNLIVRKI 194
NRS +SL+ Y+ SK+T+ L R + Q +++ I+R++
Sbjct: 241 NRSGSSLSSNFYSSRLSKKTSLASLNKSRASLQHKIMSLSRNIIRRV 287
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.1 bits (57), Expect = 2.7
Identities = 21/66 (31%), Positives = 30/66 (45%)
Frame = -3
Query: 713 PAAPSRSLTNFPTVRSSLSETKDSVAQRLSSTLRSWVWKLAASTRPHITPS*SATWTSVR 534
P+ PS S T P++ SS+ T SV+ + S L S + H+TP S
Sbjct: 120 PSIPSSSST--PSI-SSIPHTTSSVSNDIPSVLGSSDHPIDLDNPEHLTPPSSFITAKQL 176
Query: 533 TCTPTP 516
+ PTP
Sbjct: 177 SRLPTP 182
>SPAC3A12.03c |mug145||ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +2
Query: 479 GDSRVHGGTTDNTVLAYKSLRMSTSHFMM 565
GDSR H TD ++L+ S+R S +++
Sbjct: 277 GDSRTHSAETDRSLLSAMSVRNSRMPYIV 305
>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 879
Score = 25.8 bits (54), Expect = 6.2
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 2/50 (4%)
Frame = +1
Query: 505 HRQYGVGVQVLTDVHVALHDGVICGLVDAASFHTQ--ERRVEESLWATES 648
HR GV+ T V +L+ + G +AA T+ E+ VE +W T S
Sbjct: 516 HRYLPPGVEPGTPVRDSLNSVLNVGATNAAGVSTENVEQEVETPVWETFS 565
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 25.8 bits (54), Expect = 6.2
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = -3
Query: 701 SRSLTNFPTVRSSLSETKDSVAQRLSSTLRSWV 603
S S + VRSSLS+TKD+ S LR W+
Sbjct: 1513 SSSSFEYQLVRSSLSDTKDTFR---SCFLRPWI 1542
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 6.2
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -1
Query: 466 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 356
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,061,082
Number of Sequences: 5004
Number of extensions: 65960
Number of successful extensions: 215
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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