BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021162 (798 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 29 0.58 SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 27 2.4 SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 27 2.4 SPBC1198.02 |dea2||adenine deaminase Dea2|Schizosaccharomyces po... 27 2.4 SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|... 27 3.1 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 26 5.4 SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr 1||... 25 9.5 SPBC19C2.04c |ubp11||ubiquitin C-terminal hydrolase Ubp11|Schizo... 25 9.5 SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 25 9.5 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 29.5 bits (63), Expect = 0.58 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS ++ T YSP SY P + S Sbjct: 1587 PTSPSYSPTSPSYSPTS--PSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS 1642 Score = 29.1 bits (62), Expect = 0.77 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS ++ T YSP SY P + S Sbjct: 1601 PTSPSYSATSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS 1656 Score = 28.7 bits (61), Expect = 1.0 Identities = 18/58 (31%), Positives = 25/58 (43%), Gaps = 1/58 (1%) Frame = -2 Query: 482 YASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMISS 312 Y +S + PS+ S P P PTS ++ T YSP SY P + S+ Sbjct: 1551 YGLTSPSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSA 1608 Score = 28.7 bits (61), Expect = 1.0 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS ++ T YSP SY P + S Sbjct: 1615 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS 1670 Score = 28.7 bits (61), Expect = 1.0 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS ++ T YSP SY P + S Sbjct: 1629 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS 1684 Score = 28.7 bits (61), Expect = 1.0 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS ++ T YSP SY P + S Sbjct: 1643 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS 1698 Score = 28.7 bits (61), Expect = 1.0 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS ++ T YSP SY P + S Sbjct: 1657 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS 1712 Score = 28.7 bits (61), Expect = 1.0 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS ++ T YSP SY P + S Sbjct: 1671 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS 1726 Score = 28.7 bits (61), Expect = 1.0 Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS ++ T YSP SY P + S Sbjct: 1685 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS 1740 Score = 28.3 bits (60), Expect = 1.3 Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 1/54 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 327 P + S + PS +P S P S P PTS ++ T YSP SY P + Sbjct: 1699 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTS 1750 >SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizosaccharomyces pombe|chr 1|||Manual Length = 950 Score = 27.5 bits (58), Expect = 2.4 Identities = 9/27 (33%), Positives = 17/27 (62%) Frame = +3 Query: 525 GQTPVIIAGNKEQQKKYLGRLIDEPLV 605 G + ++ + E+ KKY+GR + PL+ Sbjct: 262 GDRAIAVSPHDERYKKYVGRFVKHPLI 288 >SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schizosaccharomyces pombe|chr 3|||Manual Length = 576 Score = 27.5 bits (58), Expect = 2.4 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = -1 Query: 384 SLLYNGPRVLPRLIILTRHXXDFILRE 304 S + NG RVLP I RH +I+RE Sbjct: 12 STMRNGARVLPPEAISKRHIGPYIIRE 38 >SPBC1198.02 |dea2||adenine deaminase Dea2|Schizosaccharomyces pombe|chr 2|||Manual Length = 367 Score = 27.5 bits (58), Expect = 2.4 Identities = 15/45 (33%), Positives = 20/45 (44%) Frame = -3 Query: 463 QDNHQTHPCPYHRNVLECVRSLGQLPKPSLQRATGTPPSYHIDPP 329 ++N CP L CV S+ +LP A G P S + D P Sbjct: 249 EENIMLTMCPLSNLKLRCVNSIAELPVREFLEA-GVPFSINCDDP 292 >SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1078 Score = 27.1 bits (57), Expect = 3.1 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = +2 Query: 509 GSQWTWPNSRHYCWK*GAAEEISG*TNRRTISRCL 613 G++W+W N CW G+ ISG N R L Sbjct: 487 GTEWSWQNLNTLCWAIGS---ISGAMNEEMEKRFL 518 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 26.2 bits (55), Expect = 5.4 Identities = 18/49 (36%), Positives = 22/49 (44%) Frame = -2 Query: 515 GFLAVIIPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTT 369 G IIPV+P SS+ P T + P SG P T+Q TT Sbjct: 879 GGTTTIIPVNPGNPSSSVSAPPTTSFTPGPGGSGY-PSYSNTTQGMNTT 926 >SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.4 bits (53), Expect = 9.5 Identities = 12/36 (33%), Positives = 19/36 (52%) Frame = -2 Query: 551 SSNNDGSLAKSTGFLAVIIPVHPYASSSATRQPSNT 444 SS+ND + S G L+V + Y S +PS++ Sbjct: 88 SSDNDNKICISQGILSVTLDKDSYLVSGIDAKPSSS 123 >SPBC19C2.04c |ubp11||ubiquitin C-terminal hydrolase Ubp11|Schizosaccharomyces pombe|chr 2|||Manual Length = 350 Score = 25.4 bits (53), Expect = 9.5 Identities = 18/62 (29%), Positives = 27/62 (43%), Gaps = 3/62 (4%) Frame = +3 Query: 522 LGQTPVIIAGNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDV---AGIKTRAEKKGDEWI 692 + TP+ I+ K+ + + G I A Y VT P G + A IK G++ Sbjct: 1 MSTTPLSISRAKKYKTVFKGAAILTTFAALYIVTSPSTGKRLVKNASIKGLYNVSGNDCF 60 Query: 693 LN 698 LN Sbjct: 61 LN 62 >SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosaccharomyces pombe|chr 2|||Manual Length = 2352 Score = 25.4 bits (53), Expect = 9.5 Identities = 18/73 (24%), Positives = 33/73 (45%), Gaps = 2/73 (2%) Frame = +1 Query: 364 WPVVKKAWEVGLMNGHIPEHCGGMDMGVFDGCLVAEELAYGCTGIMTARKPVDLAKLPSL 543 W + +W L + ++ G G+F G L+A + +G + + A P + PS+ Sbjct: 2074 WSAILWSWGDLLSKKDLTQNVGP---GIFAGGLIASFICFGLSYVTFAGLPAFYRQAPSI 2130 Query: 544 LLEIRSS--RRNI 576 + S +RNI Sbjct: 2131 IPAFYRSLGKRNI 2143 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,344,317 Number of Sequences: 5004 Number of extensions: 70927 Number of successful extensions: 207 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 184 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 389395636 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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