BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021162 (798 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD) iden... 69 3e-12 At3g51840.1 68416.m05685 short-chain acyl-CoA oxidase identical ... 65 6e-11 At3g06810.1 68416.m00808 acyl-CoA dehydrogenase-related low simi... 47 2e-05 At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to ac... 42 5e-04 At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 32 0.51 At2g14050.1 68415.m01563 minichromosome maintenance family prote... 31 0.89 At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger) fa... 30 1.5 At3g50590.1 68416.m05533 transducin family protein / WD-40 repea... 30 1.5 At4g08400.1 68417.m01388 proline-rich extensin-like family prote... 30 2.0 At4g23440.1 68417.m03379 expressed protein 29 3.6 At1g14910.1 68414.m01782 epsin N-terminal homology (ENTH) domain... 29 4.7 At1g13960.2 68414.m01642 WRKY family transcription factor simila... 29 4.7 At1g13960.1 68414.m01641 WRKY family transcription factor simila... 29 4.7 At5g14240.1 68418.m01664 expressed protein 28 8.3 >At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD) identical to isovaleryl-CoA-dehydrogenase precursor [Arabidopsis thaliana] GI:5596622 Length = 409 Score = 69.3 bits (162), Expect = 3e-12 Identities = 35/71 (49%), Positives = 44/71 (61%) Frame = +3 Query: 549 GNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRAEKKGDEWILNGPKMWITNGG 728 G Q++KYL +LI V A ++EP AGSDV G+K +AEK +ILNG KMW TNG Sbjct: 130 GTAAQKEKYLPKLISGEHVGALAMSEPNAGSDVVGMKCKAEKVDGGYILNGNKMWCTNGP 189 Query: 729 VANWYFVLART 761 A V A+T Sbjct: 190 SAETLVVYAKT 200 Score = 39.5 bits (88), Expect = 0.003 Identities = 25/75 (33%), Positives = 36/75 (48%), Gaps = 4/75 (5%) Frame = +1 Query: 268 DEQKALQELARKFTKDEIXXVAGQYDKTGEYPWPVVKKAWEV-GLMNGH---IPEHCGGM 435 D Q +E KF +D I A + DKT +P V W++ G N H PE GG+ Sbjct: 33 DTQLQFKESVSKFAQDNIAPHAERIDKTNSFPKDV--NLWKLMGEFNLHGITAPEEYGGL 90 Query: 436 DMGVFDGCLVAEELA 480 +G C+ EE++ Sbjct: 91 GLGYLYHCIAMEEIS 105 >At3g51840.1 68416.m05685 short-chain acyl-CoA oxidase identical to Short-chain acyl CoA oxidase [Arabidopsis thaliana] GI:5478795; contains InterPro entry IPR006089: Acyl-CoA dehydrogenase Length = 436 Score = 64.9 bits (151), Expect = 6e-11 Identities = 32/85 (37%), Positives = 45/85 (52%) Frame = +3 Query: 516 SGLGQTPVIIAGNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRAEKKGDEWIL 695 S LG + + G++ Q++KYL L VA + +TEP GSD +G+ T A K W + Sbjct: 140 SSLGMLTIALCGSEAQKEKYLPSLAQLNTVACWALTEPDNGSDASGLGTTATKVEGGWKI 199 Query: 696 NGPKMWITNGGVANWYFVLARTIRT 770 NG K WI N A+ + AR T Sbjct: 200 NGQKRWIGNSTFADLLIIFARNTTT 224 >At3g06810.1 68416.m00808 acyl-CoA dehydrogenase-related low similarity to acyl-CoA dehydrogenase [Acinetobacter sp. NCIMB9871] GI:14587418; contains Pfam profiles PF01636: Phosphotransferase enzyme family, PF00441: Acyl-CoA dehydrogenase C-terminal domain, PF02770: Acyl-CoA dehydrogenase middle domain Length = 824 Score = 46.8 bits (106), Expect = 2e-05 Identities = 26/75 (34%), Positives = 43/75 (57%), Gaps = 2/75 (2%) Frame = +3 Query: 513 ASGLGQTPVIIA-GNKEQQKKYLGRLIDEPLVAAYGVTEPG-AGSDVAGIKTRAEKKGDE 686 A G VI+ GNKEQ ++L L++ + + + +TEP A SD I+ ++GD Sbjct: 521 APDTGNMEVILRYGNKEQISEWLIPLLEGRIRSGFAMTEPQVASSDATNIECSIRRQGDS 580 Query: 687 WILNGPKMWITNGGV 731 +++NG K W T+G + Sbjct: 581 YVINGTKWW-TSGAM 594 >At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to acyl-CoA oxidase [Arabidopsis thaliana] GI:3044214 Length = 675 Score = 41.9 bits (94), Expect = 5e-04 Identities = 21/54 (38%), Positives = 33/54 (61%), Gaps = 2/54 (3%) Frame = +3 Query: 549 GNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRA--EKKGDEWILNGP 704 G +EQQKK+L ++ Y TE G GS+V G++T A + K DE++++ P Sbjct: 114 GTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHTP 167 >At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest subunit (RPB205) (RPII) (RPB1) nearly identical to P|P18616 DNA-directed RNA polymerase II largest subunit (EC 2.7.7.6) {Arabidopsis thaliana} Length = 1840 Score = 31.9 bits (69), Expect = 0.51 Identities = 21/54 (38%), Positives = 26/54 (48%), Gaps = 1/54 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 327 P + S + PS +P S P S P PTS A+ T GYSP SY P + Sbjct: 1666 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTS 1717 Score = 29.9 bits (64), Expect = 2.0 Identities = 21/58 (36%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315 P + S + PS +P S P S P PTS A+ T YSP SY P + S Sbjct: 1617 PTSPSYSPTSPSYSPTS--PSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSPTSPSYS 1672 Score = 28.7 bits (61), Expect = 4.7 Identities = 17/45 (37%), Positives = 21/45 (46%) Frame = -2 Query: 485 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 351 P + S + PS +P S P S P PTS A+ T YSP Sbjct: 1610 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPAYSPTSPAYSP 1652 Score = 28.3 bits (60), Expect = 6.2 Identities = 23/79 (29%), Positives = 31/79 (39%), Gaps = 1/79 (1%) Frame = -2 Query: 548 SNNDGSLAKSTGFLAVIIPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTT 369 S++ G S G+ P + + P +P S P S P PTS A+ T Sbjct: 1540 SSSPGYSPSSPGYSPTSPGYSPTSPGYSPTSPGYSPTS--PTYSPSSPGYSPTSPAYSPT 1597 Query: 368 GHGYSPVL-SY*PATXMIS 315 YSP SY P + S Sbjct: 1598 SPSYSPTSPSYSPTSPSYS 1616 >At2g14050.1 68415.m01563 minichromosome maintenance family protein / MCM family protein low similarity to SP|P49736 DNA replication licensing factor MCM2 {Homo sapiens}; contains Pfam profile PF00493: MCM2/3/5 family Length = 653 Score = 31.1 bits (67), Expect = 0.89 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +3 Query: 618 VTEPGAGSDVAGIKTRAEKKGDEWILNGPKMWITNGGV 731 V G GS AG+ A K G EW+L + + +GG+ Sbjct: 379 VITTGLGSTSAGLTVTAVKDGGEWMLEAGALVLADGGL 416 >At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger) family protein similar to Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) Length = 691 Score = 30.3 bits (65), Expect = 1.5 Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%) Frame = -2 Query: 497 IPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFF-TTGHGYSPV 348 + + P S +R P++TP+SIPP M P R T + T + +PV Sbjct: 309 LDLRPGQSFVVSRNPNSTPVSIPPGSRTMLPPFRWTGSSLVGGTSNSTAPV 359 >At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat family protein contains 3 WD-40 repeats (PF00400); some similarity to s-tomosyn isoform (GI:4689231)[Rattus norvegicus]; contains non-consensus AT-AC splice sites at intron 18 Length = 1606 Score = 30.3 bits (65), Expect = 1.5 Identities = 15/50 (30%), Positives = 24/50 (48%) Frame = -2 Query: 554 ISSNNDGSLAKSTGFLAVIIPVHPYASSSATRQPSNTPMSIPPQCSGMCP 405 +S + ++A +T + PV P+A SS T+QP PP + P Sbjct: 1197 LSQPSSSTVATTTAPSSASAPVDPFAMSSWTQQPQPVSQPAPPGVAAPIP 1246 >At4g08400.1 68417.m01388 proline-rich extensin-like family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 513 Score = 29.9 bits (64), Expect = 2.0 Identities = 16/56 (28%), Positives = 27/56 (48%) Frame = -3 Query: 475 APQPQDNHQTHPCPYHRNVLECVRSLGQLPKPSLQRATGTPPSYHIDPPQX*FHPS 308 +P P+ N+++ P PY+R+ S P P + + PP + PP + PS Sbjct: 248 SPSPKVNYKSPPPPYYRSPPPPYYS----PSPKVDYKSPPPPYVYSSPPPPYYSPS 299 >At4g23440.1 68417.m03379 expressed protein Length = 964 Score = 29.1 bits (62), Expect = 3.6 Identities = 9/26 (34%), Positives = 17/26 (65%) Frame = +3 Query: 654 IKTRAEKKGDEWILNGPKMWITNGGV 731 ++ EK+GD W +G ++W+ GG+ Sbjct: 231 VRDIVEKRGDLWEKHGGELWVLYGGI 256 >At1g14910.1 68414.m01782 epsin N-terminal homology (ENTH) domain-containing protein contains Pfam PF01417: ENTH domain. ENTH (Epsin N-terminal homology) domain; similar to Chain A, Calm-N N-Terminal Domain Of Clathrin Assembly Lymphoid Myeloid Leukaemia Protein, Pi(4,5)p2 Complex (GP:13399999) {Homo sapiens} Length = 692 Score = 28.7 bits (61), Expect = 4.7 Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 2/64 (3%) Frame = -2 Query: 527 AKSTGFLAV-IIPVHPYASSSATRQPSNTPMSIPPQCSGMCPF-IRPTSQAFFTTGHGYS 354 + +TGF + ++PV +++A + P+S P +G + P S+ TTG G Sbjct: 560 SNTTGFGEIPVVPVTEPPNTTAFGEFPVVPVSEPQNITGFGALPVTPASEPSNTTGFGEF 619 Query: 353 PVLS 342 PV+S Sbjct: 620 PVVS 623 >At1g13960.2 68414.m01642 WRKY family transcription factor similar to WKRY DNA-binding protein GB:AAD17441 Length = 487 Score = 28.7 bits (61), Expect = 4.7 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = -2 Query: 479 ASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 351 ++S +T PS +S+PP RP S+ FF G G+SP Sbjct: 9 STSKSTGAPSRPTLSLPP---------RPFSEMFFNGGVGFSP 42 >At1g13960.1 68414.m01641 WRKY family transcription factor similar to WKRY DNA-binding protein GB:AAD17441 Length = 514 Score = 28.7 bits (61), Expect = 4.7 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = -2 Query: 479 ASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 351 ++S +T PS +S+PP RP S+ FF G G+SP Sbjct: 9 STSKSTGAPSRPTLSLPP---------RPFSEMFFNGGVGFSP 42 >At5g14240.1 68418.m01664 expressed protein Length = 256 Score = 27.9 bits (59), Expect = 8.3 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +3 Query: 555 KEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRAEKKGDEWIL 695 +E +KK L L + V YG P +GSD T+A ++W++ Sbjct: 80 EEYRKKRLSELREAAKVKRYGTVTPISGSDFVREVTQA--SAEDWVV 124 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,623,250 Number of Sequences: 28952 Number of extensions: 396008 Number of successful extensions: 1267 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 1046 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1224 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1804564000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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