SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021162
         (798 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD) iden...    69   3e-12
At3g51840.1 68416.m05685 short-chain acyl-CoA oxidase identical ...    65   6e-11
At3g06810.1 68416.m00808 acyl-CoA dehydrogenase-related low simi...    47   2e-05
At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to ac...    42   5e-04
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ...    32   0.51 
At2g14050.1 68415.m01563 minichromosome maintenance family prote...    31   0.89 
At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger) fa...    30   1.5  
At3g50590.1 68416.m05533 transducin family protein / WD-40 repea...    30   1.5  
At4g08400.1 68417.m01388 proline-rich extensin-like family prote...    30   2.0  
At4g23440.1 68417.m03379 expressed protein                             29   3.6  
At1g14910.1 68414.m01782 epsin N-terminal homology (ENTH) domain...    29   4.7  
At1g13960.2 68414.m01642 WRKY family transcription factor simila...    29   4.7  
At1g13960.1 68414.m01641 WRKY family transcription factor simila...    29   4.7  
At5g14240.1 68418.m01664 expressed protein                             28   8.3  

>At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD)
           identical to isovaleryl-CoA-dehydrogenase precursor
           [Arabidopsis thaliana] GI:5596622
          Length = 409

 Score = 69.3 bits (162), Expect = 3e-12
 Identities = 35/71 (49%), Positives = 44/71 (61%)
 Frame = +3

Query: 549 GNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRAEKKGDEWILNGPKMWITNGG 728
           G   Q++KYL +LI    V A  ++EP AGSDV G+K +AEK    +ILNG KMW TNG 
Sbjct: 130 GTAAQKEKYLPKLISGEHVGALAMSEPNAGSDVVGMKCKAEKVDGGYILNGNKMWCTNGP 189

Query: 729 VANWYFVLART 761
            A    V A+T
Sbjct: 190 SAETLVVYAKT 200



 Score = 39.5 bits (88), Expect = 0.003
 Identities = 25/75 (33%), Positives = 36/75 (48%), Gaps = 4/75 (5%)
 Frame = +1

Query: 268 DEQKALQELARKFTKDEIXXVAGQYDKTGEYPWPVVKKAWEV-GLMNGH---IPEHCGGM 435
           D Q   +E   KF +D I   A + DKT  +P  V    W++ G  N H    PE  GG+
Sbjct: 33  DTQLQFKESVSKFAQDNIAPHAERIDKTNSFPKDV--NLWKLMGEFNLHGITAPEEYGGL 90

Query: 436 DMGVFDGCLVAEELA 480
            +G    C+  EE++
Sbjct: 91  GLGYLYHCIAMEEIS 105


>At3g51840.1 68416.m05685 short-chain acyl-CoA oxidase identical to
           Short-chain acyl CoA oxidase [Arabidopsis thaliana]
           GI:5478795; contains InterPro entry IPR006089: Acyl-CoA
           dehydrogenase
          Length = 436

 Score = 64.9 bits (151), Expect = 6e-11
 Identities = 32/85 (37%), Positives = 45/85 (52%)
 Frame = +3

Query: 516 SGLGQTPVIIAGNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRAEKKGDEWIL 695
           S LG   + + G++ Q++KYL  L     VA + +TEP  GSD +G+ T A K    W +
Sbjct: 140 SSLGMLTIALCGSEAQKEKYLPSLAQLNTVACWALTEPDNGSDASGLGTTATKVEGGWKI 199

Query: 696 NGPKMWITNGGVANWYFVLARTIRT 770
           NG K WI N   A+   + AR   T
Sbjct: 200 NGQKRWIGNSTFADLLIIFARNTTT 224


>At3g06810.1 68416.m00808 acyl-CoA dehydrogenase-related low
           similarity to acyl-CoA dehydrogenase [Acinetobacter sp.
           NCIMB9871] GI:14587418; contains Pfam profiles PF01636:
           Phosphotransferase enzyme family, PF00441: Acyl-CoA
           dehydrogenase C-terminal domain, PF02770: Acyl-CoA
           dehydrogenase middle domain
          Length = 824

 Score = 46.8 bits (106), Expect = 2e-05
 Identities = 26/75 (34%), Positives = 43/75 (57%), Gaps = 2/75 (2%)
 Frame = +3

Query: 513 ASGLGQTPVIIA-GNKEQQKKYLGRLIDEPLVAAYGVTEPG-AGSDVAGIKTRAEKKGDE 686
           A   G   VI+  GNKEQ  ++L  L++  + + + +TEP  A SD   I+    ++GD 
Sbjct: 521 APDTGNMEVILRYGNKEQISEWLIPLLEGRIRSGFAMTEPQVASSDATNIECSIRRQGDS 580

Query: 687 WILNGPKMWITNGGV 731
           +++NG K W T+G +
Sbjct: 581 YVINGTKWW-TSGAM 594


>At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to
           acyl-CoA oxidase [Arabidopsis thaliana] GI:3044214
          Length = 675

 Score = 41.9 bits (94), Expect = 5e-04
 Identities = 21/54 (38%), Positives = 33/54 (61%), Gaps = 2/54 (3%)
 Frame = +3

Query: 549 GNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRA--EKKGDEWILNGP 704
           G +EQQKK+L       ++  Y  TE G GS+V G++T A  + K DE++++ P
Sbjct: 114 GTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHTP 167


>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
            subunit (RPB205) (RPII) (RPB1) nearly identical to
            P|P18616 DNA-directed RNA polymerase II largest subunit
            (EC 2.7.7.6) {Arabidopsis thaliana}
          Length = 1840

 Score = 31.9 bits (69), Expect = 0.51
 Identities = 21/54 (38%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
 Frame = -2

Query: 485  PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 327
            P + S +   PS +P S  P  S   P   PTS A+  T  GYSP   SY P +
Sbjct: 1666 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTS 1717



 Score = 29.9 bits (64), Expect = 2.0
 Identities = 21/58 (36%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
 Frame = -2

Query: 485  PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PATXMIS 315
            P + S +   PS +P S  P  S   P   PTS A+  T   YSP   SY P +   S
Sbjct: 1617 PTSPSYSPTSPSYSPTS--PSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSPTSPSYS 1672



 Score = 28.7 bits (61), Expect = 4.7
 Identities = 17/45 (37%), Positives = 21/45 (46%)
 Frame = -2

Query: 485  PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 351
            P + S +   PS +P S  P  S   P   PTS A+  T   YSP
Sbjct: 1610 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPAYSPTSPAYSP 1652



 Score = 28.3 bits (60), Expect = 6.2
 Identities = 23/79 (29%), Positives = 31/79 (39%), Gaps = 1/79 (1%)
 Frame = -2

Query: 548  SNNDGSLAKSTGFLAVIIPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTT 369
            S++ G    S G+        P +   +   P  +P S  P  S   P   PTS A+  T
Sbjct: 1540 SSSPGYSPSSPGYSPTSPGYSPTSPGYSPTSPGYSPTS--PTYSPSSPGYSPTSPAYSPT 1597

Query: 368  GHGYSPVL-SY*PATXMIS 315
               YSP   SY P +   S
Sbjct: 1598 SPSYSPTSPSYSPTSPSYS 1616


>At2g14050.1 68415.m01563 minichromosome maintenance family protein
           / MCM family protein low similarity to SP|P49736 DNA
           replication licensing factor MCM2 {Homo sapiens};
           contains Pfam profile PF00493: MCM2/3/5 family
          Length = 653

 Score = 31.1 bits (67), Expect = 0.89
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +3

Query: 618 VTEPGAGSDVAGIKTRAEKKGDEWILNGPKMWITNGGV 731
           V   G GS  AG+   A K G EW+L    + + +GG+
Sbjct: 379 VITTGLGSTSAGLTVTAVKDGGEWMLEAGALVLADGGL 416


>At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger)
           family protein similar to Pfam domain, PF00097: Zinc
           finger, C3HC4 type (RING finger)
          Length = 691

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
 Frame = -2

Query: 497 IPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFF-TTGHGYSPV 348
           + + P  S   +R P++TP+SIPP    M P  R T  +    T +  +PV
Sbjct: 309 LDLRPGQSFVVSRNPNSTPVSIPPGSRTMLPPFRWTGSSLVGGTSNSTAPV 359


>At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat
            family protein contains 3 WD-40 repeats (PF00400); some
            similarity to s-tomosyn isoform (GI:4689231)[Rattus
            norvegicus]; contains non-consensus AT-AC splice sites at
            intron 18
          Length = 1606

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 15/50 (30%), Positives = 24/50 (48%)
 Frame = -2

Query: 554  ISSNNDGSLAKSTGFLAVIIPVHPYASSSATRQPSNTPMSIPPQCSGMCP 405
            +S  +  ++A +T   +   PV P+A SS T+QP       PP  +   P
Sbjct: 1197 LSQPSSSTVATTTAPSSASAPVDPFAMSSWTQQPQPVSQPAPPGVAAPIP 1246


>At4g08400.1 68417.m01388 proline-rich extensin-like family protein
           contains proline-rich extensin domains,
           INTERPRO:IPR002965
          Length = 513

 Score = 29.9 bits (64), Expect = 2.0
 Identities = 16/56 (28%), Positives = 27/56 (48%)
 Frame = -3

Query: 475 APQPQDNHQTHPCPYHRNVLECVRSLGQLPKPSLQRATGTPPSYHIDPPQX*FHPS 308
           +P P+ N+++ P PY+R+      S    P P +   +  PP  +  PP   + PS
Sbjct: 248 SPSPKVNYKSPPPPYYRSPPPPYYS----PSPKVDYKSPPPPYVYSSPPPPYYSPS 299


>At4g23440.1 68417.m03379 expressed protein 
          Length = 964

 Score = 29.1 bits (62), Expect = 3.6
 Identities = 9/26 (34%), Positives = 17/26 (65%)
 Frame = +3

Query: 654 IKTRAEKKGDEWILNGPKMWITNGGV 731
           ++   EK+GD W  +G ++W+  GG+
Sbjct: 231 VRDIVEKRGDLWEKHGGELWVLYGGI 256


>At1g14910.1 68414.m01782 epsin N-terminal homology (ENTH)
           domain-containing protein contains Pfam PF01417: ENTH
           domain. ENTH (Epsin N-terminal homology) domain; similar
           to Chain A, Calm-N N-Terminal Domain Of Clathrin
           Assembly Lymphoid Myeloid Leukaemia Protein, Pi(4,5)p2
           Complex (GP:13399999) {Homo sapiens}
          Length = 692

 Score = 28.7 bits (61), Expect = 4.7
 Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
 Frame = -2

Query: 527 AKSTGFLAV-IIPVHPYASSSATRQPSNTPMSIPPQCSGMCPF-IRPTSQAFFTTGHGYS 354
           + +TGF  + ++PV    +++A  +    P+S P   +G     + P S+   TTG G  
Sbjct: 560 SNTTGFGEIPVVPVTEPPNTTAFGEFPVVPVSEPQNITGFGALPVTPASEPSNTTGFGEF 619

Query: 353 PVLS 342
           PV+S
Sbjct: 620 PVVS 623


>At1g13960.2 68414.m01642 WRKY family transcription factor similar
           to WKRY DNA-binding protein GB:AAD17441
          Length = 487

 Score = 28.7 bits (61), Expect = 4.7
 Identities = 16/43 (37%), Positives = 23/43 (53%)
 Frame = -2

Query: 479 ASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 351
           ++S +T  PS   +S+PP         RP S+ FF  G G+SP
Sbjct: 9   STSKSTGAPSRPTLSLPP---------RPFSEMFFNGGVGFSP 42


>At1g13960.1 68414.m01641 WRKY family transcription factor similar
           to WKRY DNA-binding protein GB:AAD17441
          Length = 514

 Score = 28.7 bits (61), Expect = 4.7
 Identities = 16/43 (37%), Positives = 23/43 (53%)
 Frame = -2

Query: 479 ASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 351
           ++S +T  PS   +S+PP         RP S+ FF  G G+SP
Sbjct: 9   STSKSTGAPSRPTLSLPP---------RPFSEMFFNGGVGFSP 42


>At5g14240.1 68418.m01664 expressed protein
          Length = 256

 Score = 27.9 bits (59), Expect = 8.3
 Identities = 15/47 (31%), Positives = 24/47 (51%)
 Frame = +3

Query: 555 KEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRAEKKGDEWIL 695
           +E +KK L  L +   V  YG   P +GSD     T+A    ++W++
Sbjct: 80  EEYRKKRLSELREAAKVKRYGTVTPISGSDFVREVTQA--SAEDWVV 124


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,623,250
Number of Sequences: 28952
Number of extensions: 396008
Number of successful extensions: 1267
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1046
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1224
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1804564000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -