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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021157
         (626 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati...   105   2e-23
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati...   101   3e-22
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2...   101   5e-22
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1...   101   5e-22
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1...   101   5e-22
At4g26340.1 68417.m03787 F-box family protein contains F-box dom...    27   7.7  

>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 2 (Methionine
           adenosyltransferase 2, AdoMet synthetase 2)
           [Catharanthus roseus] SWISS-PROT:Q96552
          Length = 393

 Score =  105 bits (253), Expect = 2e-23
 Identities = 57/125 (45%), Positives = 75/125 (60%), Gaps = 2/125 (1%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+KANVDY+++VR+T + IG+  +  G D   C V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609
           GVH +  +  EEVGA DQG MFGYAT            L T    KL    +     W R
Sbjct: 106 GVHGHLTKKPEEVGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLR 165

Query: 610 PDSKT 624
           PD KT
Sbjct: 166 PDGKT 170



 Score = 81.4 bits (192), Expect = 4e-16
 Identities = 42/70 (60%), Positives = 49/70 (70%), Gaps = 4/70 (5%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACE--TKLKPVWCF--CVAKSHP 298
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACE  TK   V  F     K++ 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63

Query: 299 KLTWIIKKLC 328
               I++K C
Sbjct: 64  DYEQIVRKTC 73


>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 3 (Methionine
           adenosyltransferase 3, AdoMet synthetase 3)
           [Lycopersicon esculentum] SWISS-PROT:P43282
          Length = 390

 Score =  101 bits (243), Expect = 3e-22
 Identities = 54/125 (43%), Positives = 74/125 (59%), Gaps = 2/125 (1%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+ A VDY+K+VR T + IG+  +  G D   C+V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609
           GVH +  +  E++GA DQG MFGYAT            L T    KL    + +   W R
Sbjct: 106 GVHGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLR 165

Query: 610 PDSKT 624
           PD KT
Sbjct: 166 PDGKT 170



 Score = 79.8 bits (188), Expect = 1e-15
 Identities = 36/44 (81%), Positives = 39/44 (88%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET  K
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTK 47


>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
           identical to S-adenosylmethionine synthetase 2
           (Methionine adenosyltransferase 2, AdoMet synthetase 2)
           [Arabidopsis thaliana] SWISS-PROT:P17562
          Length = 393

 Score =  101 bits (241), Expect = 5e-22
 Identities = 53/125 (42%), Positives = 73/125 (58%), Gaps = 2/125 (1%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+KA +DY+K+VR+T + IG+     G D   C V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609
           GVH +  +  E++GA DQG MFGYAT            L T    +L    +     W R
Sbjct: 106 GVHGHFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKIGARLTEVRKNGTCRWLR 165

Query: 610 PDSKT 624
           PD KT
Sbjct: 166 PDGKT 170



 Score = 81.0 bits (191), Expect = 6e-16
 Identities = 36/44 (81%), Positives = 39/44 (88%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET  K
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47


>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =  101 bits (241), Expect = 5e-22
 Identities = 55/125 (44%), Positives = 73/125 (58%), Gaps = 2/125 (1%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+KA VDY+K+VR+T + IG+     G D   C V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609
           GVH +  +  EE+GA DQG MFGYAT            L T    +L    +     W R
Sbjct: 106 GVHGHFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLR 165

Query: 610 PDSKT 624
           PD KT
Sbjct: 166 PDGKT 170



 Score = 81.0 bits (191), Expect = 6e-16
 Identities = 36/44 (81%), Positives = 39/44 (88%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET  K
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47


>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =  101 bits (241), Expect = 5e-22
 Identities = 55/125 (44%), Positives = 73/125 (58%), Gaps = 2/125 (1%)
 Frame = +1

Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435
           TKT MV++ GEIT+KA VDY+K+VR+T + IG+     G D   C V++ ++QQSP+IA 
Sbjct: 46  TKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQ 105

Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609
           GVH +  +  EE+GA DQG MFGYAT            L T    +L    +     W R
Sbjct: 106 GVHGHFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLR 165

Query: 610 PDSKT 624
           PD KT
Sbjct: 166 PDGKT 170



 Score = 81.0 bits (191), Expect = 6e-16
 Identities = 36/44 (81%), Positives = 39/44 (88%)
 Frame = +2

Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET  K
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47


>At4g26340.1 68417.m03787 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 419

 Score = 27.5 bits (58), Expect = 7.7
 Identities = 20/61 (32%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
 Frame = +1

Query: 337 VKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHENRN-DEEVGARDQGLMFGYATVR 513
           V  + YDDS    DYK+ S  +     S N     H + N   +  A D GL  G+A  R
Sbjct: 41  VSELEYDDSYHTGDYKSFSQFVYRSLLSNNAPVIKHLHLNLGPDCPAIDIGLWIGFALTR 100

Query: 514 Q 516
           +
Sbjct: 101 R 101


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,338,980
Number of Sequences: 28952
Number of extensions: 341904
Number of successful extensions: 937
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 926
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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