BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021157 (626 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 105 2e-23 At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 101 3e-22 At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 101 5e-22 At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 101 5e-22 At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 101 5e-22 At4g26340.1 68417.m03787 F-box family protein contains F-box dom... 27 7.7 >At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, AdoMet synthetase 2) [Catharanthus roseus] SWISS-PROT:Q96552 Length = 393 Score = 105 bits (253), Expect = 2e-23 Identities = 57/125 (45%), Positives = 75/125 (60%), Gaps = 2/125 (1%) Frame = +1 Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435 TKT MV++ GEIT+KANVDY+++VR+T + IG+ + G D C V++ ++QQSP+IA Sbjct: 46 TKTNMVMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQ 105 Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609 GVH + + EEVGA DQG MFGYAT L T KL + W R Sbjct: 106 GVHGHLTKKPEEVGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLR 165 Query: 610 PDSKT 624 PD KT Sbjct: 166 PDGKT 170 Score = 81.4 bits (192), Expect = 4e-16 Identities = 42/70 (60%), Positives = 49/70 (70%), Gaps = 4/70 (5%) Frame = +2 Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACE--TKLKPVWCF--CVAKSHP 298 FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACE TK V F K++ Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63 Query: 299 KLTWIIKKLC 328 I++K C Sbjct: 64 DYEQIVRKTC 73 >At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 3 (Methionine adenosyltransferase 3, AdoMet synthetase 3) [Lycopersicon esculentum] SWISS-PROT:P43282 Length = 390 Score = 101 bits (243), Expect = 3e-22 Identities = 54/125 (43%), Positives = 74/125 (59%), Gaps = 2/125 (1%) Frame = +1 Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435 TKT MV++ GEIT+ A VDY+K+VR T + IG+ + G D C+V++ ++QQSP+IA Sbjct: 46 TKTNMVMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQ 105 Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609 GVH + + E++GA DQG MFGYAT L T KL + + W R Sbjct: 106 GVHGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLR 165 Query: 610 PDSKT 624 PD KT Sbjct: 166 PDGKT 170 Score = 79.8 bits (188), Expect = 1e-15 Identities = 36/44 (81%), Positives = 39/44 (88%) Frame = +2 Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262 FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET K Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTK 47 >At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2) identical to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, AdoMet synthetase 2) [Arabidopsis thaliana] SWISS-PROT:P17562 Length = 393 Score = 101 bits (241), Expect = 5e-22 Identities = 53/125 (42%), Positives = 73/125 (58%), Gaps = 2/125 (1%) Frame = +1 Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435 TKT MV++ GEIT+KA +DY+K+VR+T + IG+ G D C V++ ++QQSP+IA Sbjct: 46 TKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQ 105 Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609 GVH + + E++GA DQG MFGYAT L T +L + W R Sbjct: 106 GVHGHFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKIGARLTEVRKNGTCRWLR 165 Query: 610 PDSKT 624 PD KT Sbjct: 166 PDGKT 170 Score = 81.0 bits (191), Expect = 6e-16 Identities = 36/44 (81%), Positives = 39/44 (88%) Frame = +2 Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET K Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47 >At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT:P23686 Length = 393 Score = 101 bits (241), Expect = 5e-22 Identities = 55/125 (44%), Positives = 73/125 (58%), Gaps = 2/125 (1%) Frame = +1 Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435 TKT MV++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA Sbjct: 46 TKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQ 105 Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609 GVH + + EE+GA DQG MFGYAT L T +L + W R Sbjct: 106 GVHGHFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLR 165 Query: 610 PDSKT 624 PD KT Sbjct: 166 PDGKT 170 Score = 81.0 bits (191), Expect = 6e-16 Identities = 36/44 (81%), Positives = 39/44 (88%) Frame = +2 Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET K Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47 >At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT:P23686 Length = 393 Score = 101 bits (241), Expect = 5e-22 Identities = 55/125 (44%), Positives = 73/125 (58%), Gaps = 2/125 (1%) Frame = +1 Query: 256 TKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAA 435 TKT MV++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA Sbjct: 46 TKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQ 105 Query: 436 GVHEN--RNDEEVGARDQGLMFGYATVRQKNACR*L*CLHTNSIRKLQSSGEMENFWWAR 609 GVH + + EE+GA DQG MFGYAT L T +L + W R Sbjct: 106 GVHGHFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLR 165 Query: 610 PDSKT 624 PD KT Sbjct: 166 PDGKT 170 Score = 81.0 bits (191), Expect = 6e-16 Identities = 36/44 (81%), Positives = 39/44 (88%) Frame = +2 Query: 131 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETKLK 262 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET K Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47 >At4g26340.1 68417.m03787 F-box family protein contains F-box domain Pfam:PF00646 Length = 419 Score = 27.5 bits (58), Expect = 7.7 Identities = 20/61 (32%), Positives = 27/61 (44%), Gaps = 1/61 (1%) Frame = +1 Query: 337 VKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHENRN-DEEVGARDQGLMFGYATVR 513 V + YDDS DYK+ S + S N H + N + A D GL G+A R Sbjct: 41 VSELEYDDSYHTGDYKSFSQFVYRSLLSNNAPVIKHLHLNLGPDCPAIDIGLWIGFALTR 100 Query: 514 Q 516 + Sbjct: 101 R 101 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,338,980 Number of Sequences: 28952 Number of extensions: 341904 Number of successful extensions: 937 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 900 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 926 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1275599520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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