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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021154
         (632 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g19900.1 68415.m02326 malate oxidoreductase, putative similar...   127   5e-30
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar...   122   2e-28
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar...   120   8e-28
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar...   117   6e-27
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar...    98   5e-21
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar...    93   2e-19
At3g19895.1 68416.m02519 expressed protein                             29   1.9  
At1g15240.1 68414.m01824 phox (PX) domain-containing protein wea...    29   2.6  
At4g08160.1 68417.m01347 glycosyl hydrolase family 10 protein / ...    29   3.4  
At3g07780.1 68416.m00949 expressed protein                             27   7.8  

>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP:P51615) {Vitis vinifera}
          Length = 581

 Score =  127 bits (307), Expect = 5e-30
 Identities = 55/91 (60%), Positives = 72/91 (79%)
 Frame = +2

Query: 236 RQIRKSINKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG 415
           RQ +  + KY+ L  L +RNE LFY+ + DNV E++PIVYTPTVG ACQKFG ++RRP+G
Sbjct: 90  RQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQG 149

Query: 416 LFITIHDKGHVYDVLKNWPETDVRAIVVTDG 508
           LFI++ DKG + DVLKNWPE +++ IVVTDG
Sbjct: 150 LFISLKDKGKILDVLKNWPERNIQVIVVTDG 180



 Score = 74.1 bits (174), Expect = 7e-14
 Identities = 32/41 (78%), Positives = 37/41 (90%)
 Frame = +1

Query: 508 KRILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 630
           +RILGLGDLG  GMGIPVGKLALY+ALGG++P  CLP+TID
Sbjct: 181 ERILGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTID 221


>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP:P12628) {Phaseolus vulgaris}
          Length = 588

 Score =  122 bits (294), Expect = 2e-28
 Identities = 50/91 (54%), Positives = 71/91 (78%)
 Frame = +2

Query: 236 RQIRKSINKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG 415
           RQ    + +Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G +YRRP+G
Sbjct: 97  RQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQG 156

Query: 416 LFITIHDKGHVYDVLKNWPETDVRAIVVTDG 508
           L+I++ +KG + +VLKNWP+  ++ IVVTDG
Sbjct: 157 LYISLKEKGKILEVLKNWPQRGIQVIVVTDG 187



 Score = 75.4 bits (177), Expect = 3e-14
 Identities = 34/41 (82%), Positives = 37/41 (90%)
 Frame = +1

Query: 508 KRILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 630
           +RILGLGDLG  GMGIPVGKL+LYTALGGI+P  CLPITID
Sbjct: 188 ERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITID 228



 Score = 30.3 bits (65), Expect = 1.1
 Identities = 15/33 (45%), Positives = 20/33 (60%)
 Frame = +1

Query: 79  TSGLSGLDHLKHPGLNKGMAFTIEERHSWAYTG 177
           TS  SG   ++ P  NKG+AFT +ER +   TG
Sbjct: 44  TSVASGYTLMRDPRYNKGLAFTDKERDAHYITG 76


>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP|P12628) {Phaseolus vulgaris}
          Length = 588

 Score =  120 bits (289), Expect = 8e-28
 Identities = 48/91 (52%), Positives = 72/91 (79%)
 Frame = +2

Query: 236 RQIRKSINKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG 415
           RQ    + +Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+G
Sbjct: 97  RQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQG 156

Query: 416 LFITIHDKGHVYDVLKNWPETDVRAIVVTDG 508
           L+I++++KG + +VLKNWP+  ++ IVVTDG
Sbjct: 157 LYISLNEKGKILEVLKNWPQRGIQVIVVTDG 187



 Score = 75.4 bits (177), Expect = 3e-14
 Identities = 34/41 (82%), Positives = 37/41 (90%)
 Frame = +1

Query: 508 KRILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 630
           +RILGLGDLG  GMGIPVGKL+LYTALGGI+P  CLPITID
Sbjct: 188 ERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITID 228



 Score = 30.3 bits (65), Expect = 1.1
 Identities = 15/33 (45%), Positives = 20/33 (60%)
 Frame = +1

Query: 79  TSGLSGLDHLKHPGLNKGMAFTIEERHSWAYTG 177
           TS  SG   ++ P  NKG+AFT +ER +   TG
Sbjct: 44  TSVASGYTLMRDPRYNKGLAFTDKERDAHYLTG 76


>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
           malate oxidoreductase (NADP-dependent malic enzyme)
           GB:P34105 (Populus balsamifera subsp. trichocarpa)
          Length = 646

 Score =  117 bits (282), Expect = 6e-27
 Identities = 46/91 (50%), Positives = 72/91 (79%)
 Frame = +2

Query: 236 RQIRKSINKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG 415
           RQ +  + KY+ +M L + NE LFY+ + D+V E++P++YTPTVG ACQK+G ++ RP+G
Sbjct: 155 RQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQG 214

Query: 416 LFITIHDKGHVYDVLKNWPETDVRAIVVTDG 508
           LFI++ +KG +++VL+NWPE +++ IVVTDG
Sbjct: 215 LFISLKEKGKIHEVLRNWPEKNIQVIVVTDG 245



 Score = 74.5 bits (175), Expect = 5e-14
 Identities = 32/41 (78%), Positives = 37/41 (90%)
 Frame = +1

Query: 508 KRILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 630
           +RILGLGDLG  GMGIPVGKL+LYTALGG++P  CLP+TID
Sbjct: 246 ERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTID 286



 Score = 31.9 bits (69), Expect = 0.36
 Identities = 16/43 (37%), Positives = 24/43 (55%)
 Frame = +3

Query: 129 GHGFYHRRASLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 257
           G  F HR      + GLLPP V +Q+ QV+    ++ +Y+ PL
Sbjct: 119 GLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPL 161


>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
           NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
           precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
           tuberosum}
          Length = 607

 Score = 97.9 bits (233), Expect = 5e-21
 Identities = 41/87 (47%), Positives = 59/87 (67%)
 Frame = +2

Query: 251 SINKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITI 430
           ++ K+  L  L DRNE L+YR + DN+ +  PI+YTPTVGL CQ +  +YRRPRG++ + 
Sbjct: 102 ALAKWRMLNRLHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSA 161

Query: 431 HDKGHVYDVLKNWPETDVRAIVVTDGN 511
            DKG +  ++ NWP   V  IV+TDG+
Sbjct: 162 KDKGEMMSMIYNWPAPQVDMIVITDGS 188



 Score = 57.6 bits (133), Expect = 6e-09
 Identities = 25/40 (62%), Positives = 30/40 (75%)
 Frame = +1

Query: 511 RILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 630
           RILGLGDLG  G+GIP+GKL +Y A  GI P + LPI +D
Sbjct: 189 RILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLD 228



 Score = 28.7 bits (61), Expect = 3.4
 Identities = 17/39 (43%), Positives = 19/39 (48%)
 Frame = +3

Query: 135 GFYHRRASLLGIHGLLPPRVKTQEEQVELCKLSIDRYEN 251
           GF       LGI GLLPPRV T  +Q +    S    EN
Sbjct: 53  GFPLTERDRLGIRGLLPPRVMTCVQQCDRFIESFRSLEN 91


>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
           NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
           precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
           tuberosum}
          Length = 623

 Score = 92.7 bits (220), Expect = 2e-19
 Identities = 39/87 (44%), Positives = 59/87 (67%)
 Frame = +2

Query: 251 SINKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITI 430
           ++ K+  L  L DRNE ++Y+ + +N+ E  PIVYTPTVGL CQ +  ++RRPRG++ + 
Sbjct: 109 ALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA 168

Query: 431 HDKGHVYDVLKNWPETDVRAIVVTDGN 511
            D+G +  ++ NWP   V  IVVTDG+
Sbjct: 169 EDRGEMMSMVYNWPAEQVDMIVVTDGS 195



 Score = 56.0 bits (129), Expect = 2e-08
 Identities = 26/40 (65%), Positives = 29/40 (72%)
 Frame = +1

Query: 511 RILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 630
           RILGLGDLG  G+GI VGKL LY A  GI P + LP+ ID
Sbjct: 196 RILGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMID 235



 Score = 29.1 bits (62), Expect = 2.6
 Identities = 13/22 (59%), Positives = 15/22 (68%)
 Frame = +1

Query: 94  GLDHLKHPGLNKGMAFTIEERH 159
           GLD L  P  NKG AFT+ ER+
Sbjct: 46  GLDILHDPWFNKGTAFTMTERN 67


>At3g19895.1 68416.m02519 expressed protein
          Length = 554

 Score = 29.5 bits (63), Expect = 1.9
 Identities = 15/42 (35%), Positives = 23/42 (54%)
 Frame = -1

Query: 596 MPPSAV*SASLPTGMPIPHAPKSPRPRIRFRRLQQWHGRPSP 471
           +P S + SA+ P+G PI     +P  R+R   +  + G PSP
Sbjct: 37  IPVSPI-SAAYPSGDPILMMSGTPLARVRLSEILPYEGAPSP 77


>At1g15240.1 68414.m01824 phox (PX) domain-containing protein weak
           similarity to SP|Q9Y5W8 Sorting nexin 13 {Homo sapiens};
           contains Pfam profiles PF00787: PX domain, PF02194: PXA
           domain
          Length = 706

 Score = 29.1 bits (62), Expect = 2.6
 Identities = 22/69 (31%), Positives = 30/69 (43%), Gaps = 4/69 (5%)
 Frame = +1

Query: 25  SEMERDRIGLXGSG----DGQPTSGLSGLDHLKHPGLNKGMAFTIEERHSWAYTGCYHHE 192
           SE E    GL   G    DG+    L G+  + HP  N G +     +    Y     H+
Sbjct: 490 SEDEETVTGLNSPGTRVWDGRTKKNL-GVSRIHHPLENSGRSLKKTSKGHERYQQVPGHQ 548

Query: 193 SRRKRSRLN 219
           S RKRSR++
Sbjct: 549 SGRKRSRIS 557


>At4g08160.1 68417.m01347 glycosyl hydrolase family 10 protein /
           carbohydrate-binding domain-containing protein  ;
           contains Pfam profiles PF00331: Glycosyl hydrolase
           family 10, PF02018: Carbohydrate binding domain
          Length = 752

 Score = 28.7 bits (61), Expect = 3.4
 Identities = 14/50 (28%), Positives = 23/50 (46%)
 Frame = -3

Query: 558 WDAHPACAQVAKTQNTFPSVTTMARTSVSGQFLRTSYTCPLSWIVMKRPL 409
           +D  P   +V +T N+FP  T + RT +  +     +T   +W V    L
Sbjct: 394 FDLFPYIVKVKQTYNSFPVGTCINRTDIDNEDFVDFFTKNFNWAVFGNEL 443


>At3g07780.1 68416.m00949 expressed protein
          Length = 566

 Score = 27.5 bits (58), Expect = 7.8
 Identities = 12/26 (46%), Positives = 12/26 (46%)
 Frame = +1

Query: 448 IRCS*ELAGDGRPCHCCNRRKRILGL 525
           I C  EL  DG PC  C  RK    L
Sbjct: 200 IACQNELPADGCPCEICANRKGFCNL 225


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,314,873
Number of Sequences: 28952
Number of extensions: 354637
Number of successful extensions: 1079
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1022
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1078
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1295224128
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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