Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021152X
(538 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-rel... 32 0.21
At3g06180.1 68416.m00710 expressed protein 31 0.49
At5g12080.2 68418.m01415 mechanosensitive ion channel domain-con... 30 0.85
At5g12080.1 68418.m01414 mechanosensitive ion channel domain-con... 30 0.85
At5g08410.1 68418.m00990 ferredoxin-thioredoxin reductase, putat... 30 0.85
At3g56100.1 68416.m06235 leucine-rich repeat transmembrane prote... 29 1.5
At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein heli... 29 1.5
At2g25470.1 68415.m03050 leucine-rich repeat family protein cont... 29 2.0
At2g34930.1 68415.m04288 disease resistance family protein conta... 28 4.5
At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger) fa... 28 4.5
At1g12460.1 68414.m01440 leucine-rich repeat transmembrane prote... 28 4.5
At2g25730.1 68415.m03084 expressed protein 27 6.0
At5g57930.1 68418.m07246 expressed protein 27 7.9
At3g13270.1 68416.m01670 hypothetical protein contains similarit... 27 7.9
At2g31900.1 68415.m03897 myosin family protein contains Pfam pro... 27 7.9
>At4g02660.1 68417.m00361 WD-40 repeat family protein /
beige-related contains Pfam PF00400: WD domain, G-beta
repeat; similar to BEIGE (GI:3928547) [Rattus
norvegicus]; lysosomal trafficking regulator - Bos
taurus, EMBL: AF114785
Length = 3471
Score = 32.3 bits (70), Expect = 0.21
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = +2
Query: 425 LLDDTSREKAKLEIDLKRLYEE 490
LL TSR+K KLE+D KR +EE
Sbjct: 57 LLSPTSRDKLKLELDFKRYWEE 78
>At3g06180.1 68416.m00710 expressed protein
Length = 241
Score = 31.1 bits (67), Expect = 0.49
Identities = 22/62 (35%), Positives = 31/62 (50%), Gaps = 4/62 (6%)
Frame = -2
Query: 519 LV*AFFRSSFSSYNL----FKSISSLAFSRDVSSNSIPGILEFMLVHSFNVGNLAGDHLL 352
L+ ++F F S++L F S+SSL FS D +SIP F F ++A L
Sbjct: 68 LLSSYFSYLFHSFSLLSSHFPSLSSLIFSDDEDLSSIPPASYFFAFAVFFAASIAFLDLC 127
Query: 351 CG 346
CG
Sbjct: 128 CG 129
>At5g12080.2 68418.m01415 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein contains Pfam profile PF00924:
Mechanosensitive ion channel
Length = 734
Score = 30.3 bits (65), Expect = 0.85
Identities = 23/84 (27%), Positives = 39/84 (46%)
Frame = +2
Query: 227 RQSRLQEKDDYKISTTGWLPISIKYASWRVRIRDYVVKYKPHRRWSPARFPTLKECTSMN 406
R + E ++ IS + P+S K A + RI +Y+ + H W+P +KE +MN
Sbjct: 625 RSPNMGETVEFSISFS--TPVS-KIAHLKERIAEYLEQNPQH--WAPVHSVVVKEIENMN 679
Query: 407 SKMPGILLDDTSREKAKLEIDLKR 478
+ D T + E +L+R
Sbjct: 680 KLKMALYSDHTITFQENRERNLRR 703
>At5g12080.1 68418.m01414 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein contains Pfam profile PF00924:
Mechanosensitive ion channel
Length = 734
Score = 30.3 bits (65), Expect = 0.85
Identities = 23/84 (27%), Positives = 39/84 (46%)
Frame = +2
Query: 227 RQSRLQEKDDYKISTTGWLPISIKYASWRVRIRDYVVKYKPHRRWSPARFPTLKECTSMN 406
R + E ++ IS + P+S K A + RI +Y+ + H W+P +KE +MN
Sbjct: 625 RSPNMGETVEFSISFS--TPVS-KIAHLKERIAEYLEQNPQH--WAPVHSVVVKEIENMN 679
Query: 407 SKMPGILLDDTSREKAKLEIDLKR 478
+ D T + E +L+R
Sbjct: 680 KLKMALYSDHTITFQENRERNLRR 703
>At5g08410.1 68418.m00990 ferredoxin-thioredoxin reductase, putative
similar to ferredoxin-thioredoxin reductase, variable
chain (FTR-V, Ferredoxin- thioredoxin reductase subunit
A, FTR-A) [Zea mays] SWISS-PROT:P80680
Length = 184
Score = 30.3 bits (65), Expect = 0.85
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = -3
Query: 413 SWSSCSYIPLMLETSRVTTSCVVCISRRNPEFSL 312
S SS ++P+ L TS + C ISR+NP+ ++
Sbjct: 37 SSSSSIFVPISLSTSYGRSKCAFSISRKNPKSTI 70
>At3g56100.1 68416.m06235 leucine-rich repeat transmembrane protein
kinase, putative hypothetical proteins - Arabidopsis
thaliana
Length = 719
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = -2
Query: 465 ISSLAFSRDVSSNSIPGILEFMLVHSFNVGNLAGDHLLCGLYFTT 331
+ SL F +VS N++ G + +L FN + G+ LLCG +T
Sbjct: 304 LESLNFF-NVSYNNLSGPVPTLLSQKFNSSSFVGNSLLCGYSVST 347
>At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein
helicase, putative similar to SP|O75643 U5 small nuclear
ribonucleoprotein 200 kDa helicase {Homo sapiens};
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF02889:
Sec63 domain
Length = 2171
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/35 (45%), Positives = 22/35 (62%)
Frame = +1
Query: 262 NLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVT 366
NL++RL Y VR+L + S REI+ TQ +VT
Sbjct: 578 NLSNRLKDYGVIVRELSGDQSLTGREIEETQIIVT 612
>At2g25470.1 68415.m03050 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to disease
resistance protein [Lycopersicon esculentum]
gi|3894383|gb|AAC78591
Length = 910
Score = 29.1 bits (62), Expect = 2.0
Identities = 18/45 (40%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = -2
Query: 477 LFKSISSLAFSRDVSSNSIPGIL-EFMLVHSFNVGNLAGDHLLCG 346
L S++SLA DVSSN++ GI+ + ++F + G+ LLCG
Sbjct: 788 LLSSLTSLAVF-DVSSNNLSGIIPQGRQFNTFEEESYLGNPLLCG 831
>At2g34930.1 68415.m04288 disease resistance family protein contains
leucine rich-repeat domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.1 [Lycopersicon
pimpinellifolium] gi|1184075|gb|AAC15779
Length = 905
Score = 27.9 bits (59), Expect = 4.5
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = -2
Query: 228 LVGLSGLLGRPAELEGRLPTGVLDCSGLCSDD 133
L LS LL LEG++P + +CSGL + D
Sbjct: 662 LPSLSVLLLNQNSLEGKIPESLRNCSGLTNID 693
>At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 383
Score = 27.9 bits (59), Expect = 4.5
Identities = 25/76 (32%), Positives = 35/76 (46%), Gaps = 2/76 (2%)
Frame = +2
Query: 281 LPISIKYASWRVRIRDYVVKYKPHRRWSPARFPTLKECTSMNSKMPGILL--DDTSREKA 454
L IS++ W V D K R A + KE ++ + I L DD REK
Sbjct: 236 LVISLETTKWEVADAD-----KEFRWLKSAVSSSEKEYEQISRRTDDIKLELDDERREKK 290
Query: 455 KLEIDLKRLYEENDDL 502
KLE +L L +E ++L
Sbjct: 291 KLEEELMELNKELEEL 306
>At1g12460.1 68414.m01440 leucine-rich repeat transmembrane protein
kinase, putative
Length = 882
Score = 27.9 bits (59), Expect = 4.5
Identities = 13/29 (44%), Positives = 15/29 (51%)
Frame = -2
Query: 231 CLVGLSGLLGRPAELEGRLPTGVLDCSGL 145
C L L EL GR+PTGV+ C L
Sbjct: 282 CSESLEFLDASSNELTGRIPTGVMGCKSL 310
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 27.5 bits (58), Expect = 6.0
Identities = 16/58 (27%), Positives = 28/58 (48%)
Frame = +1
Query: 256 LQNLNDRLAAYIDKVRQLESENSGLRREIQTTQEVVTREVSNIKGMYEHELQDARNTV 429
+Q L DR+ I + + E ENSGL ++ Q V+ V +K + + + N +
Sbjct: 103 VQVLIDRVTERIKEDEESEDENSGLGNCLRVLQGVLELGVERLKFVVDTSSSEGSNKI 160
>At5g57930.1 68418.m07246 expressed protein
Length = 440
Score = 27.1 bits (57), Expect = 7.9
Identities = 25/91 (27%), Positives = 50/91 (54%), Gaps = 2/91 (2%)
Frame = +2
Query: 263 ISTTGWLPISIKYASWRVRIRDYVVKYKPHR-RWSPA-RFPTLKECTSMNSKMPGILLDD 436
IS +G+ P S+ +A V R ++ +R +SP+ + +++ +NS++ +L
Sbjct: 8 ISFSGFSPKSVPFAIHSVTRRQFLNPNTFYRFGFSPSLQGSSIEFSLQLNSRV--VL--- 62
Query: 437 TSREKAKLEIDLKRLYEENDDLKKA*TRRQK 529
S+E+ L + ++ +N+DL K TRR+K
Sbjct: 63 -SKERRSLPLVVRNDRPQNEDLPKQYTRREK 92
>At3g13270.1 68416.m01670 hypothetical protein contains similarity
to replication protein A1
Length = 570
Score = 27.1 bits (57), Expect = 7.9
Identities = 15/29 (51%), Positives = 18/29 (62%)
Frame = -3
Query: 416 ASWSSCSYIPLMLETSRVTTSCVVCISRR 330
AS S IPLMLE S+ TT V +S+R
Sbjct: 441 ASIVSGDQIPLMLEYSQETTDTVTPVSKR 469
>At2g31900.1 68415.m03897 myosin family protein contains Pfam
profiles: PF00063 myosin head (motor domain), PF01843 DIL
domain, PF00612 IQ calmodulin-binding motif, PF02736
myosin N-terminal SH3-like domain
Length = 1556
Score = 27.1 bits (57), Expect = 7.9
Identities = 16/48 (33%), Positives = 29/48 (60%), Gaps = 3/48 (6%)
Frame = +1
Query: 292 DKVRQLESENSGLRREIQTTQEVV--TREVSNIKGM-YEHELQDARNT 426
DKV LESEN LRR+ ++ ++ + R ++ K + EH+ ++ + T
Sbjct: 1067 DKVAILESENETLRRQTESAEKTMPPARVFASEKNLENEHQTKEIQAT 1114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,525,036
Number of Sequences: 28952
Number of extensions: 206363
Number of successful extensions: 621
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 604
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 621
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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