Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021148
(725 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60600.1 68416.m06781 vesicle-associated membrane protein, pu... 52 4e-07
At2g45140.1 68415.m05618 vesicle-associated membrane protein, pu... 51 7e-07
At1g08820.1 68414.m00982 vesicle-associated membrane family prot... 49 4e-06
At5g47180.2 68418.m05818 vesicle-associated membrane family prot... 48 6e-06
At5g47180.1 68418.m05817 vesicle-associated membrane family prot... 48 6e-06
At2g23830.1 68415.m02847 vesicle-associated membrane protein, pu... 44 1e-04
At4g00170.1 68417.m00018 vesicle-associated membrane family prot... 44 1e-04
At1g51270.1 68414.m05766 vesicle-associated membrane protein, pu... 40 0.002
At1g79710.1 68414.m09296 integral membrane transporter family pr... 31 0.59
At3g57710.1 68416.m06429 protein kinase family protein contains ... 29 3.1
At1g06320.1 68414.m00668 hypothetical protein 29 3.1
At2g25440.1 68415.m03047 leucine-rich repeat family protein cont... 29 4.1
At5g11820.1 68418.m01380 expressed protein self-incompatibility ... 28 7.2
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 28 7.2
At4g21450.2 68417.m03102 vesicle-associated membrane family prot... 27 9.6
At4g21450.1 68417.m03103 vesicle-associated membrane family prot... 27 9.6
>At3g60600.1 68416.m06781 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 256
Score = 52.0 bits (119), Expect = 4e-07
Identities = 27/57 (47%), Positives = 38/57 (66%)
Frame = +2
Query: 83 NQVLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPN 253
+++LT+EP +L+F + C+ Y LTN T++ V FK+KTT PKKYCVRPN
Sbjct: 20 SELLTVEPL-DLQFPFELKK--QISCSLY--LTNKTDNNVAFKVKTTNPKKYCVRPN 71
Score = 36.7 bits (81), Expect = 0.016
Identities = 28/86 (32%), Positives = 43/86 (50%), Gaps = 1/86 (1%)
Frame = +1
Query: 163 NLHETNEPDKRYSAIQDQNNSTKEILRASQFGVLAPNSKQEIAITPQPVYLDPNENH-KH 339
+L+ TN+ D + N K +R + GV+ P S E+ +T Q P++ K
Sbjct: 43 SLYLTNKTDNNVAFKVKTTNPKKYCVRPNT-GVVLPRSTCEVLVTMQAQKEAPSDMQCKD 101
Query: 340 KFMVQSVIAPEGKTNIDQVWKEISPE 417
KF++Q VIA G T KE++PE
Sbjct: 102 KFLLQGVIASPGVTA-----KEVTPE 122
>At2g45140.1 68415.m05618 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 239
Score = 51.2 bits (117), Expect = 7e-07
Identities = 28/59 (47%), Positives = 38/59 (64%)
Frame = +2
Query: 77 MPNQVLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPN 253
M N++LTI+P +L+F + C+ Y L N T++ V FK+KTT PKKYCVRPN
Sbjct: 1 MSNELLTIDPV-DLQFPFELKK--QISCSLY--LGNKTDNYVAFKVKTTNPKKYCVRPN 54
>At1g08820.1 68414.m00982 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 386
Score = 48.8 bits (111), Expect = 4e-06
Identities = 26/56 (46%), Positives = 38/56 (67%)
Frame = +2
Query: 89 VLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPNL 256
+L I+P+ L+F V+ + C ++LTN T+ V FK+KTT+PKKYCVRPN+
Sbjct: 5 LLDIQPRT-LQFAVDLKK--QTSCV--VQLTNTTHHYVAFKVKTTSPKKYCVRPNV 55
>At5g47180.2 68418.m05818 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 48.0 bits (109), Expect = 6e-06
Identities = 28/59 (47%), Positives = 40/59 (67%), Gaps = 2/59 (3%)
Frame = +2
Query: 83 NQVLTIEPQNELKFKVNFSGLFEHGCTTY--MRLTNPTNDTVLFKIKTTAPKKYCVRPN 253
NQ+++I+P +ELKF LFE +Y +++ N T + V FK+KTT+PKKY VRPN
Sbjct: 7 NQLISIQP-DELKF------LFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPN 58
>At5g47180.1 68418.m05817 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 48.0 bits (109), Expect = 6e-06
Identities = 28/59 (47%), Positives = 40/59 (67%), Gaps = 2/59 (3%)
Frame = +2
Query: 83 NQVLTIEPQNELKFKVNFSGLFEHGCTTY--MRLTNPTNDTVLFKIKTTAPKKYCVRPN 253
NQ+++I+P +ELKF LFE +Y +++ N T + V FK+KTT+PKKY VRPN
Sbjct: 7 NQLISIQP-DELKF------LFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPN 58
>At2g23830.1 68415.m02847 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 149
Score = 44.0 bits (99), Expect = 1e-04
Identities = 25/57 (43%), Positives = 33/57 (57%)
Frame = +2
Query: 83 NQVLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPN 253
N++L IEP L+F + C+ Y LTN T++ V FK+KTT YCVRPN
Sbjct: 4 NELLEIEPMY-LQFPFELKK--QMSCSLY--LTNKTDNNVAFKVKTTNRNNYCVRPN 55
Score = 35.5 bits (78), Expect = 0.036
Identities = 27/111 (24%), Positives = 53/111 (47%), Gaps = 4/111 (3%)
Frame = +1
Query: 163 NLHETNEPDKRYSAIQDQNNSTKEILRASQFGVLAPNSKQEIAITPQPVYLDPNENHK-H 339
+L+ TN+ D + N +R + +G++ P S ++ +T Q P++
Sbjct: 27 SLYLTNKTDNNVAFKVKTTNRNNYCVRPN-YGLILPKSTCKVLVTMQAQKEVPSDMQSFE 85
Query: 340 KFMVQSVIAPEGKTNID---QVWKEISPEQLMDYKLKCVFETPRGTNLNDS 483
KFM+QSV+A G T + +++ + S + + KL+ + TN+ S
Sbjct: 86 KFMIQSVLASPGVTAKEVTREMFSKESGHVVEETKLRVTYVCSTTTNITSS 136
>At4g00170.1 68417.m00018 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 239
Score = 43.6 bits (98), Expect = 1e-04
Identities = 26/56 (46%), Positives = 33/56 (58%), Gaps = 1/56 (1%)
Frame = +2
Query: 89 VLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTN-DTVLFKIKTTAPKKYCVRPN 253
++ I P ELKF + C+ M+LTN T V FK+KTT P+KYCVRPN
Sbjct: 6 LVNIHP-TELKFPFELKK--QSSCS--MQLTNKTTTQCVAFKVKTTNPRKYCVRPN 56
>At1g51270.1 68414.m05766 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 289
Score = 39.9 bits (89), Expect = 0.002
Identities = 23/61 (37%), Positives = 38/61 (62%)
Frame = +2
Query: 74 KMPNQVLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPN 253
+M +++L I+P +++F + + + C+ + LTN T + V FK KTT KKY VRPN
Sbjct: 123 RMSDELLIIDPV-DVQFPIELNK--KVSCS--LNLTNKTENYVAFKAKTTNAKKYYVRPN 177
Query: 254 L 256
+
Sbjct: 178 V 178
>At1g79710.1 68414.m09296 integral membrane transporter family
protein similar to high affinity folic acid/methotrexate
transporter 5 (GI:21898554) [Leishmania tarentolae];
Interpro IPR001991/ PR00173 Sodium:dicarboxylater
symporter family
Length = 497
Score = 31.5 bits (68), Expect = 0.59
Identities = 30/123 (24%), Positives = 53/123 (43%), Gaps = 4/123 (3%)
Frame = -3
Query: 384 VGFSFWSYYTLDHKFMLVVFIGVQIYRLRSNCDFLFRIWRQNSKLGRTQYFFGAVVLILN 205
VG + + + F VVF + + L D + + R N KLG YFF V ++
Sbjct: 317 VGILLYQNFLKNFPFRNVVFWALSLSVLSGFLDLIL-VLRINLKLGLPDYFFIVVDEFVS 375
Query: 204 STVSFVGFVSLM*VVQP-CSKSPEK---LTLNFNSFCGSIVSTWLGILLYYKHSISLAKF 37
+S + ++ L+ + C E L G +VS+W G +L + ++ +F
Sbjct: 376 HMISRIKWLPLLVLSSKLCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHALKVTRTQF 435
Query: 36 NRL 28
+ L
Sbjct: 436 DNL 438
>At3g57710.1 68416.m06429 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 351
Score = 29.1 bits (62), Expect = 3.1
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +1
Query: 292 ITPQPVYLDPNENHKHKFMVQSVIAPEGKTNIDQVW 399
+ P V+LD N K + S+ PEGK+ I+ W
Sbjct: 192 VKPMHVFLDKNWTAKLSDLSFSISLPEGKSRIEAEW 227
>At1g06320.1 68414.m00668 hypothetical protein
Length = 180
Score = 29.1 bits (62), Expect = 3.1
Identities = 15/47 (31%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = +1
Query: 403 EIS-PEQLMDYKLKCVFETPRGTNLNDSGDNAAQNDAPRKELQSQKT 540
EIS P+Q + + K + TN D DN P K+++++KT
Sbjct: 100 EISKPKQKSNKRKKATMKKSLNTNFGDENDNTMMMMLPTKKMRNKKT 146
>At2g25440.1 68415.m03047 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to NL0E
[Lycopersicon esculentum] gi|4235643|gb|AAD13303
Length = 671
Score = 28.7 bits (61), Expect = 4.1
Identities = 21/72 (29%), Positives = 31/72 (43%), Gaps = 2/72 (2%)
Frame = -3
Query: 348 HKFMLVVFIGVQIYRLRSNCDFLFR-IWRQNSKLGRTQYFFGAV-VLILNSTVSFVGFVS 175
HKF + + R N F IW NSK+ Y +G V V + N TV+ F+
Sbjct: 47 HKFQALTQFKNEFDTRRCNHSNYFNGIWCDNSKVRSQSYDYGTVSVELSNQTVASSSFII 106
Query: 174 LM*VVQPCSKSP 139
++ + SP
Sbjct: 107 FATLISLTTTSP 118
>At5g11820.1 68418.m01380 expressed protein self-incompatibility
protein S3 precursor, Papaver rhoeas cv., PIR:S69186
Length = 175
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -1
Query: 329 FSLGSKYTGCGVIAISCFEFGARTPNWDARN 237
F L + +T C +IA +CF + A ++ RN
Sbjct: 11 FRLATAFTWCLLIASACFAYTASAARFEVRN 41
>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
(Alpha-tocopherol associated protein) (TAP) (bTAP)
(Fragment) (SP:P58875) {Bos taurus}
Length = 683
Score = 27.9 bits (59), Expect = 7.2
Identities = 29/128 (22%), Positives = 50/128 (39%), Gaps = 5/128 (3%)
Frame = +1
Query: 172 ETNEPDKRYSAIQDQNNSTKEILRASQFGVLAPNSKQEIAITPQPVYLDP-----NENHK 336
ET E +K SA + KE + A+ ++A K+E + P PV P E K
Sbjct: 154 ETKEEEK--SAAPATVETKKEEILAAPAPIVAETKKEETPVAPAPVETKPAAPVVAETKK 211
Query: 337 HKFMVQSVIAPEGKTNIDQVWKEISPEQLMDYKLKCVFETPRGTNLNDSGDNAAQNDAPR 516
+ + + + E K V E +P + + K + T + AA + +
Sbjct: 212 EEILPAAPVTTETKVEEKVVPVETTPAAPVTTETKEEEKAAPVTTETKEEEKAAPGETKK 271
Query: 517 KELQSQKT 540
+E + T
Sbjct: 272 EEKATAST 279
>At4g21450.2 68417.m03102 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 212
Score = 27.5 bits (58), Expect = 9.6
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 170 MRLTNPTNDTVLFKIKTTAPKKYCVRP 250
+++ N + V FK +TTAPK +RP
Sbjct: 130 IKIKNTSKSHVAFKFQTTAPKSCFMRP 156
>At4g21450.1 68417.m03103 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 295
Score = 27.5 bits (58), Expect = 9.6
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 170 MRLTNPTNDTVLFKIKTTAPKKYCVRP 250
+++ N + V FK +TTAPK +RP
Sbjct: 130 IKIKNTSKSHVAFKFQTTAPKSCFMRP 156
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,343,965
Number of Sequences: 28952
Number of extensions: 312666
Number of successful extensions: 940
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 908
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 940
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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