BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021146 (654 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g60900.1 68414.m06856 U2 snRNP auxiliary factor large subunit... 31 0.67 At3g08670.1 68416.m01007 expressed protein 30 1.2 At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 30 1.5 At5g49400.1 68418.m06113 zinc knuckle (CCHC-type) family protein... 29 2.7 At1g65670.1 68414.m07452 cytochrome P450 family protein similar ... 29 3.6 At1g79690.1 68414.m09294 MutT/nudix family protein contains Pfam... 28 6.2 At5g59120.1 68418.m07409 subtilase family protein contains simil... 27 8.2 At2g44190.1 68415.m05497 expressed protein contains Pfam profil... 27 8.2 >At1g60900.1 68414.m06856 U2 snRNP auxiliary factor large subunit, putative similar to U2 snRNP auxiliary factor, large subunit GB:CAA77136 from [Nicotiana plumbaginifolia] Length = 589 Score = 31.1 bits (67), Expect = 0.67 Identities = 25/74 (33%), Positives = 32/74 (43%), Gaps = 11/74 (14%) Frame = -3 Query: 352 QSREQGHQDHHRGPAPRHSEK-------HSASEADRAERFCRERSETPRHRNKP---SRP 203 + R++ H+D HR + SEK H DR R R+R RHR + SR Sbjct: 98 RQRDRHHRDRHRDRSRERSEKRDDLDDDHHRRSRDRDRRRSRDRDREVRHRRRSRSRSRS 157 Query: 202 VWSRRS-PHHFHCS 164 RRS H H S Sbjct: 158 RSERRSRSEHRHKS 171 >At3g08670.1 68416.m01007 expressed protein Length = 567 Score = 30.3 bits (65), Expect = 1.2 Identities = 14/44 (31%), Positives = 23/44 (52%) Frame = -3 Query: 337 GHQDHHRGPAPRHSEKHSASEADRAERFCRERSETPRHRNKPSR 206 G+ H AP+ + AS A +A R +SE+ H ++P+R Sbjct: 110 GNDSHSSLAAPKIASSARASSASKASRLSVSQSESGYHSSRPAR 153 >At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to RNA helicase [Rattus norvegicus] GI:897915; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1166 Score = 29.9 bits (64), Expect = 1.5 Identities = 16/55 (29%), Positives = 24/55 (43%), Gaps = 3/55 (5%) Frame = -3 Query: 361 RAVQSREQGHQDHHRG---PAPRHSEKHSASEADRAERFCRERSETPRHRNKPSR 206 R V +E+GH++H R R E+ + +R RER E R + R Sbjct: 114 RRVNEKERGHREHERDRGKDRKRDREREERKDKEREREKDRERREREREEREKER 168 >At5g49400.1 68418.m06113 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 275 Score = 29.1 bits (62), Expect = 2.7 Identities = 20/83 (24%), Positives = 32/83 (38%) Frame = -3 Query: 463 ELELDHSRALSKSRLSSWXXXXXXXXXXXXXVTLRAVQSREQGHQDHHRGPAPRHSEKHS 284 E + D S S S SS + Q + + + + + SE S Sbjct: 191 ETDSDGSSGESSSEYSSSSDSEDERRRRRKAKKSKKKQKQRKERRRRYSSSSSESSESES 250 Query: 283 ASEADRAERFCRERSETPRHRNK 215 AS++D E R + ++ RH NK Sbjct: 251 ASDSDSDEDRSRRKKKSKRHSNK 273 >At1g65670.1 68414.m07452 cytochrome P450 family protein similar to Cytochrome P450 90A1 (SP:Q42569) [Arabidopsis thaliana] Length = 482 Score = 28.7 bits (61), Expect = 3.6 Identities = 14/30 (46%), Positives = 17/30 (56%) Frame = +2 Query: 293 LAVPGRRASVVVLMSLLTALHRPEGDRRGC 382 L G + SV+ + LLT H EG RRGC Sbjct: 137 LGSQGLKLSVMQDIDLLTRTHMEEGARRGC 166 >At1g79690.1 68414.m09294 MutT/nudix family protein contains Pfam NUDIX domain [PF00293]; very low similarity to Chain A and Chain B of Escherichia coli isopentenyl diphosphate:dimethylallyl diphosphate isomerase [gi:15826361] [gi:15826360] Length = 772 Score = 27.9 bits (59), Expect = 6.2 Identities = 8/22 (36%), Positives = 14/22 (63%) Frame = -1 Query: 486 DSRRCWPKSWSLTIAGHLASRD 421 D + WP W ++ AGH+++ D Sbjct: 54 DDKDSWPGQWDISSAGHISAGD 75 >At5g59120.1 68418.m07409 subtilase family protein contains similarity to prepro-cucumisin GI:807698 from [Cucumis melo]; non-consensus AA acceptor site at exon 6 Length = 732 Score = 27.5 bits (58), Expect = 8.2 Identities = 18/55 (32%), Positives = 24/55 (43%), Gaps = 2/55 (3%) Frame = +1 Query: 172 GNGVDSVETKQVEK--VYSGDGASLSAPGRNAQLCRLRTPSVSRCAWAPGLCGGP 330 G V++ E K + VY AS + +A LC L SR +CGGP Sbjct: 340 GKSVNAYEMKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGP 394 >At2g44190.1 68415.m05497 expressed protein contains Pfam profile: PF04484 family of unknown function (DUF566) Length = 474 Score = 27.5 bits (58), Expect = 8.2 Identities = 13/43 (30%), Positives = 19/43 (44%) Frame = -3 Query: 310 APRHSEKHSASEADRAERFCRERSETPRHRNKPSRPVWSRRSP 182 +PRH +H + A+R R+ N+PS S SP Sbjct: 66 SPRHHHQHQNQRSTSAQRMRRQLKMQEGDENRPSETARSLDSP 108 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,782,603 Number of Sequences: 28952 Number of extensions: 228006 Number of successful extensions: 754 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 726 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 753 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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