Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021145
(767 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9JI78 Cluster: Peptide-N(4)-(N-acetyl-beta-glucosaminy... 56 1e-06
UniRef50_Q96IV0 Cluster: Peptide-N(4)-(N-acetyl-beta-glucosaminy... 55 2e-06
UniRef50_Q7KRR5 Cluster: Peptide-N(4)-(N-acetyl-beta-glucosaminy... 52 1e-05
UniRef50_UPI0000D569DB Cluster: PREDICTED: similar to N-glycanas... 52 2e-05
UniRef50_A7SSF4 Cluster: Predicted protein; n=1; Nematostella ve... 50 5e-05
UniRef50_Q16G66 Cluster: Peptide n-glycanase; n=2; Culicidae|Rep... 48 3e-04
UniRef50_UPI0000660009 Cluster: Peptide-N(4)-(N-acetyl-beta-gluc... 46 0.001
UniRef50_UPI00015B6364 Cluster: PREDICTED: hypothetical protein;... 40 0.068
UniRef50_Q1JTJ3 Cluster: SET-domain protein, putative; n=1; Toxo... 40 0.068
UniRef50_Q9TW67 Cluster: Peptide-N(4)-(N-acetyl-beta-glucosaminy... 40 0.068
UniRef50_UPI000051A79D Cluster: PREDICTED: similar to N-glycanas... 39 0.12
UniRef50_UPI00006D0DD4 Cluster: hypothetical protein TTHERM_0013... 35 2.6
UniRef50_A7RLQ4 Cluster: Predicted protein; n=1; Nematostella ve... 35 2.6
UniRef50_UPI00004D7437 Cluster: KARP-1-binding protein; n=4; Xen... 34 4.5
UniRef50_Q0V9Q8 Cluster: LOC779465 protein; n=1; Xenopus tropica... 34 4.5
UniRef50_Q7VRY7 Cluster: Acetyl-CoA synthetase; n=3; Bordetella|... 34 4.5
UniRef50_A4GK26 Cluster: Glycerol-3-phosphate acyltransferase; n... 33 7.8
UniRef50_Q9FW41 Cluster: Putative uncharacterized protein P0036D... 33 7.8
>UniRef50_Q9JI78 Cluster:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase; n=23; Eukaryota|Rep:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase - Mus musculus (Mouse)
Length = 651
Score = 55.6 bits (128), Expect = 1e-06
Identities = 32/89 (35%), Positives = 42/89 (47%)
Frame = +3
Query: 15 MRYYCGADRWRRACDGAETAPXSGWHAAALHATNVFRKVEPDWLQSYIAREEGEDFGSIS 194
+RY DR+ R D SGW ++FRKVE DW Y+AR+EG F IS
Sbjct: 492 LRYDIVRDRYIRVSDN--NINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYIS 549
Query: 195 WAFAASEELTCASLSIKVRTRCTRAAAST 281
W F C S +KV T R ++ +
Sbjct: 550 WKF------ECGSAGLKVDTVSIRTSSQS 572
Score = 47.6 bits (108), Expect = 3e-04
Identities = 27/62 (43%), Positives = 33/62 (53%), Gaps = 3/62 (4%)
Frame = +2
Query: 260 YESGRIDWTVKFDDENPTTVTLSDKPTKFARKFR---KVIIKAELSGGDGPVRWQHAQLF 430
+ESG + W K E L DK + F +V ++AELS GDG V WQH QLF
Sbjct: 573 FESGSVRW--KLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLF 630
Query: 431 RQ 436
RQ
Sbjct: 631 RQ 632
>UniRef50_Q96IV0 Cluster:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase; n=17; Tetrapoda|Rep:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase - Homo sapiens (Human)
Length = 654
Score = 55.2 bits (127), Expect = 2e-06
Identities = 30/82 (36%), Positives = 42/82 (51%)
Frame = +3
Query: 36 DRWRRACDGAETAPXSGWHAAALHATNVFRKVEPDWLQSYIAREEGEDFGSISWAFAASE 215
DR+ R + +T SGW ++FRKVE DW Y+AR+EG F ISW F
Sbjct: 502 DRYVRVSNNNQTI--SGWENGVWKMESIFRKVETDWHMVYLARKEGSSFAYISWKF---- 555
Query: 216 ELTCASLSIKVRTRCTRAAAST 281
C S+ +KV + R ++ T
Sbjct: 556 --ECGSVGLKVDSISIRTSSQT 575
Score = 44.8 bits (101), Expect = 0.002
Identities = 23/61 (37%), Positives = 36/61 (59%), Gaps = 2/61 (3%)
Frame = +2
Query: 260 YESGRIDWTVKFDDENPTTVTLSDKPTKFA--RKFRKVIIKAELSGGDGPVRWQHAQLFR 433
+++G ++W ++ D +T + +A +VI++AELS GDG V WQH QLFR
Sbjct: 576 FQTGTVEWKLRSDTAQ-VELTGDNSLHSYADFSGATEVILEAELSRGDGDVAWQHTQLFR 634
Query: 434 Q 436
Q
Sbjct: 635 Q 635
>UniRef50_Q7KRR5 Cluster:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase; n=3; Sophophora|Rep:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase - Drosophila melanogaster (Fruit fly)
Length = 631
Score = 52.4 bits (120), Expect = 1e-05
Identities = 27/71 (38%), Positives = 36/71 (50%), Gaps = 4/71 (5%)
Frame = +3
Query: 15 MRYYCGADRWRR-ACDGAETAPXSG---WHAAALHATNVFRKVEPDWLQSYIAREEGEDF 182
+RY C D + R A +G W A + N+FRKVE DW +Y+AR E D
Sbjct: 481 VRYSCATDTYERYAKEGEHITILDSYKTWQKAQFSSKNIFRKVERDWKMAYLARLEDTDC 540
Query: 183 GSISWAFAASE 215
G I+W F S+
Sbjct: 541 GEIAWTFDFSK 551
Score = 37.5 bits (83), Expect = 0.36
Identities = 17/31 (54%), Positives = 20/31 (64%)
Frame = +2
Query: 371 IKAELSGGDGPVRWQHAQLFRQHTYSKRSSF 463
I A+L+GG G V WQH QLFRQ S+ F
Sbjct: 594 IVAKLTGGKGDVAWQHTQLFRQSLNSRDYPF 624
>UniRef50_UPI0000D569DB Cluster: PREDICTED: similar to N-glycanase
1; n=1; Tribolium castaneum|Rep: PREDICTED: similar to
N-glycanase 1 - Tribolium castaneum
Length = 591
Score = 52.0 bits (119), Expect = 2e-05
Identities = 24/63 (38%), Positives = 31/63 (49%)
Frame = +3
Query: 15 MRYYCGADRWRRACDGAETAPXSGWHAAALHATNVFRKVEPDWLQSYIAREEGEDFGSIS 194
++Y D++ +GW +NVFRK E DW Y+AR EG D GSIS
Sbjct: 435 IKYSTAFDKYEFVKGDNSVLGVNGWQYGVYDFSNVFRKEEKDWKTVYLARNEGTDQGSIS 494
Query: 195 WAF 203
W F
Sbjct: 495 WRF 497
Score = 50.0 bits (114), Expect = 6e-05
Identities = 30/74 (40%), Positives = 41/74 (55%)
Frame = +2
Query: 251 HALYESGRIDWTVKFDDENPTTVTLSDKPTKFARKFRKVIIKAELSGGDGPVRWQHAQLF 430
+ LYESG + + DD++ + T+ R IKA LSGG G V WQHAQLF
Sbjct: 516 YQLYESGVVQIQLISDDKSEGALE-----TQAFSGVRHFTIKATLSGGKGNVAWQHAQLF 570
Query: 431 RQHTYSKRSSFIVT 472
RQ + SK F+++
Sbjct: 571 RQSSDSKDFPFVLS 584
>UniRef50_A7SSF4 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 636
Score = 50.4 bits (115), Expect = 5e-05
Identities = 23/63 (36%), Positives = 37/63 (58%), Gaps = 4/63 (6%)
Frame = +2
Query: 260 YESGRIDWTVKFDDENPTTVTLSDKPTKFA----RKFRKVIIKAELSGGDGPVRWQHAQL 427
+E+G++DW++ DD+ + L +K R + + + A++SGG G WQHAQL
Sbjct: 557 FENGKVDWSIHGDDKEKQFIKLEGGESKQTLTRLRGAKTLTLTAQMSGGQGDNAWQHAQL 616
Query: 428 FRQ 436
FRQ
Sbjct: 617 FRQ 619
Score = 48.0 bits (109), Expect = 3e-04
Identities = 25/71 (35%), Positives = 36/71 (50%), Gaps = 4/71 (5%)
Frame = +3
Query: 15 MRYYCGADRWRRACDG---AETAPX-SGWHAAALHATNVFRKVEPDWLQSYIAREEGEDF 182
+RY D + R D + ++P W + ++FRKVE DW +Y+AR EG
Sbjct: 470 IRYSIAIDNYYRLTDDNLPSHSSPALEDWKSGTNGVESIFRKVEHDWKMAYLARNEGCQT 529
Query: 183 GSISWAFAASE 215
GS+SW SE
Sbjct: 530 GSVSWKVDVSE 540
>UniRef50_Q16G66 Cluster: Peptide n-glycanase; n=2; Culicidae|Rep:
Peptide n-glycanase - Aedes aegypti (Yellowfever
mosquito)
Length = 633
Score = 48.0 bits (109), Expect = 3e-04
Identities = 28/91 (30%), Positives = 41/91 (45%), Gaps = 5/91 (5%)
Frame = +3
Query: 15 MRYYCGADRWRRACDGAE----TAPXSGWHAAALHATNVFRKVEPDWLQSYIAREEGEDF 182
+RY C D + R G W + + NVFRK E DW Y+AR EG
Sbjct: 481 VRYSCAKDIYERFLKGPAGPLTIETTMDWKSRQYTSENVFRKEEHDWKMVYLARTEGTAV 540
Query: 183 GSISWAFAAS-EELTCASLSIKVRTRCTRAA 272
G++SW F S + + + +K+ T+ A
Sbjct: 541 GTVSWKFDFSIQGMRVRDVQVKMGTQTYEGA 571
>UniRef50_UPI0000660009 Cluster:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase (EC 3.5.1.52) (PNGase) (hPNGase)
(Peptide:N-glycanase) (N-glycanase 1).; n=1; Takifugu
rubripes|Rep:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase (EC 3.5.1.52) (PNGase) (hPNGase)
(Peptide:N-glycanase) (N-glycanase 1). - Takifugu
rubripes
Length = 664
Score = 46.0 bits (104), Expect = 0.001
Identities = 24/63 (38%), Positives = 30/63 (47%)
Frame = +3
Query: 15 MRYYCGADRWRRACDGAETAPXSGWHAAALHATNVFRKVEPDWLQSYIAREEGEDFGSIS 194
+RY D + R + +E W +VFRK+E DW YIAR EG G IS
Sbjct: 506 VRYSSVKDEYCRVSNNSEVIQT--WDRCLWKKESVFRKIEHDWEMVYIARTEGSSEGRIS 563
Query: 195 WAF 203
W F
Sbjct: 564 WKF 566
Score = 45.2 bits (102), Expect = 0.002
Identities = 29/77 (37%), Positives = 41/77 (53%), Gaps = 7/77 (9%)
Frame = +2
Query: 260 YESGRIDWTVKFDDENPTTVTLSDKPTKFARKFRKV------IIKAELSGGDGPVRWQHA 421
++SGR+ W ++ PTT S + F+ V +I AEL GGDG WQH+
Sbjct: 587 FQSGRVCWRLQ---AGPTTTEFSGDGN--VQSFQNVAGSPELVITAELGGGDGDASWQHS 641
Query: 422 QLFRQ-HTYSKRSSFIV 469
QLFRQ +++ SF V
Sbjct: 642 QLFRQSQKHTEEYSFEV 658
>UniRef50_UPI00015B6364 Cluster: PREDICTED: hypothetical protein;
n=1; Nasonia vitripennis|Rep: PREDICTED: hypothetical
protein - Nasonia vitripennis
Length = 645
Score = 39.9 bits (89), Expect = 0.068
Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 9/70 (12%)
Frame = +2
Query: 254 ALYESGRIDWTVK--FDDENPT----TVTLSDKP---TKFARKFRKVIIKAELSGGDGPV 406
A + G + W V+ + +EN V++ + TK + K+ + A LSGG+G
Sbjct: 555 ATFHGGSVKWQVEGIYSNENTKGESKVVSILESENFETKDLKGAIKINVTAVLSGGEGES 614
Query: 407 RWQHAQLFRQ 436
WQHAQLFRQ
Sbjct: 615 AWQHAQLFRQ 624
Score = 37.5 bits (83), Expect = 0.36
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +3
Query: 117 VFRKVEPDWLQSYIAREEGEDFGSISWAFAASEELTCASLSIKVRTR 257
+F K E DW Y+A+ G G I+WAF + S+K++ +
Sbjct: 507 IFTKKEEDWKMIYLAKSPGSIVGKITWAFVVEDNENMYINSVKLQAK 553
>UniRef50_Q1JTJ3 Cluster: SET-domain protein, putative; n=1;
Toxoplasma gondii RH|Rep: SET-domain protein, putative -
Toxoplasma gondii RH
Length = 4382
Score = 39.9 bits (89), Expect = 0.068
Identities = 19/46 (41%), Positives = 30/46 (65%), Gaps = 4/46 (8%)
Frame = +3
Query: 378 PSCLAATAPCAGNTPSC----SGNTRTPREAPLSSQSWSSKSNVAS 503
PSC A++ CA ++PSC S ++ T A L+S SW+S S+V++
Sbjct: 1261 PSCAASSPSCAASSPSCAALSSSSSSTSASASLTSSSWTSFSSVSA 1306
>UniRef50_Q9TW67 Cluster:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase; n=2; Caenorhabditis|Rep:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
amidase - Caenorhabditis elegans
Length = 606
Score = 39.9 bits (89), Expect = 0.068
Identities = 18/43 (41%), Positives = 24/43 (55%)
Frame = +3
Query: 75 PXSGWHAAALHATNVFRKVEPDWLQSYIAREEGEDFGSISWAF 203
P G+ A A NV R E DW +Y+ R+ G+ G+ISW F
Sbjct: 462 PEIGFSAQAFELENVQRVEETDWNMTYLCRKRGDAPGNISWHF 504
>UniRef50_UPI000051A79D Cluster: PREDICTED: similar to N-glycanase
1; n=1; Apis mellifera|Rep: PREDICTED: similar to
N-glycanase 1 - Apis mellifera
Length = 589
Score = 39.1 bits (87), Expect = 0.12
Identities = 16/25 (64%), Positives = 19/25 (76%)
Frame = +2
Query: 362 KVIIKAELSGGDGPVRWQHAQLFRQ 436
K+I+ A +SGG G WQHAQLFRQ
Sbjct: 547 KLILTATVSGGQGDCAWQHAQLFRQ 571
Score = 33.1 bits (72), Expect = 7.8
Identities = 18/50 (36%), Positives = 20/50 (40%), Gaps = 1/50 (2%)
Frame = +3
Query: 57 DGAETAPXSGWHAAALHATN-VFRKVEPDWLQSYIAREEGEDFGSISWAF 203
DG SGW +FRKVE DW Y+ R G I W F
Sbjct: 467 DGKILTNISGWENGTNEIEGGMFRKVEHDWKIVYLCRSTTAISGKIRWCF 516
>UniRef50_UPI00006D0DD4 Cluster: hypothetical protein TTHERM_00138480;
n=1; Tetrahymena thermophila SB210|Rep: hypothetical
protein TTHERM_00138480 - Tetrahymena thermophila SB210
Length = 4016
Score = 34.7 bits (76), Expect = 2.6
Identities = 15/48 (31%), Positives = 28/48 (58%)
Frame = +2
Query: 617 FFYKRQIIPQQVMENFYFYNRTSKVIVLLIDTYINVRQNRTRTIMSEK 760
F KR I+ + + +F T+K+++ LIDT IN++Q + +E+
Sbjct: 3759 FAIKRMIMMDESVYKCFFEYSTNKIVIFLIDTLINIKQQFDKNDSNEE 3806
>UniRef50_A7RLQ4 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 638
Score = 34.7 bits (76), Expect = 2.6
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = -2
Query: 364 LPKLSCELRWFIAQRDRGRVFIVELHRPVDAAALVQRVRTFIDKLAHVSSSLAANAHEIE 185
L K ++RW A++ R VFI + P++ + +RTF KL H+ + N E+E
Sbjct: 30 LHKCETQIRWRSARKGRSGVFIGN-NTPLNEMGMHDCIRTF--KLKHIKGNHLVNFSEVE 86
Query: 184 PKS 176
KS
Sbjct: 87 IKS 89
>UniRef50_UPI00004D7437 Cluster: KARP-1-binding protein; n=4;
Xenopus tropicalis|Rep: KARP-1-binding protein - Xenopus
tropicalis
Length = 1505
Score = 33.9 bits (74), Expect = 4.5
Identities = 15/38 (39%), Positives = 24/38 (63%)
Frame = +2
Query: 251 HALYESGRIDWTVKFDDENPTTVTLSDKPTKFARKFRK 364
HAL +G +T++FD+ +P VT+ D TKF +R+
Sbjct: 266 HAL-GAGHASFTIEFDENSPGKVTIKDHVTKFTPDYRQ 302
>UniRef50_Q0V9Q8 Cluster: LOC779465 protein; n=1; Xenopus
tropicalis|Rep: LOC779465 protein - Xenopus tropicalis
(Western clawed frog) (Silurana tropicalis)
Length = 928
Score = 33.9 bits (74), Expect = 4.5
Identities = 15/38 (39%), Positives = 24/38 (63%)
Frame = +2
Query: 251 HALYESGRIDWTVKFDDENPTTVTLSDKPTKFARKFRK 364
HAL +G +T++FD+ +P VT+ D TKF +R+
Sbjct: 245 HAL-GAGHASFTIEFDENSPGKVTIKDHVTKFTPDYRQ 281
>UniRef50_Q7VRY7 Cluster: Acetyl-CoA synthetase; n=3;
Bordetella|Rep: Acetyl-CoA synthetase - Bordetella
pertussis
Length = 729
Score = 33.9 bits (74), Expect = 4.5
Identities = 18/63 (28%), Positives = 32/63 (50%)
Frame = -2
Query: 529 RIPDIRFSLLATLDLLDHDCDDKGASLGVRVLPEQLGVLPAHGAVAARQLGFDDDLPKLS 350
R+PD ++ + + + S+G++ LPE++ PA A A +LGF L +S
Sbjct: 481 RLPDTAHAVTPGMQYSETEAKRLVRSIGIQTLPERIAQDPAQAAAIATELGFPVALKIVS 540
Query: 349 CEL 341
E+
Sbjct: 541 PEI 543
>UniRef50_A4GK26 Cluster: Glycerol-3-phosphate acyltransferase; n=3;
environmental samples|Rep: Glycerol-3-phosphate
acyltransferase - uncultured marine bacterium
HF130_81H07
Length = 689
Score = 33.1 bits (72), Expect = 7.8
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 593 SKI*KNIMFFYKRQIIPQQVMENFYFYNRTSKVI 694
SK KN+M FYK I+ ++E++ FY K++
Sbjct: 577 SKAEKNLMEFYKNNILHLLILESYIFYKSRKKIV 610
>UniRef50_Q9FW41 Cluster: Putative uncharacterized protein
P0036D10.2; n=1; Oryza sativa (japonica
cultivar-group)|Rep: Putative uncharacterized protein
P0036D10.2 - Oryza sativa subsp. japonica (Rice)
Length = 105
Score = 33.1 bits (72), Expect = 7.8
Identities = 20/54 (37%), Positives = 29/54 (53%)
Frame = +3
Query: 375 KPSCLAATAPCAGNTPSCSGNTRTPREAPLSSQSWSSKSNVASREKRISGIRPR 536
+PS +A T+ +TPSC PR+ P SS++W +S + S IS R R
Sbjct: 46 RPSSMATTSSSLASTPSC------PRDLPSSSRTW-RRSLLTSWRPSISSTRSR 92
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 704,384,496
Number of Sequences: 1657284
Number of extensions: 14573590
Number of successful extensions: 46319
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 44167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46296
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 64204279620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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