BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021141 (733 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|ch... 109 4e-25 SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 54 3e-08 SPAC23H3.05c |swd1||COMPASS complex subunit Swd1|Schizosaccharom... 28 1.2 SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei... 27 2.1 SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 27 2.8 SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 26 6.4 SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 26 6.4 SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 26 6.4 >SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 341 Score = 109 bits (262), Expect = 4e-25 Identities = 53/97 (54%), Positives = 68/97 (70%), Gaps = 1/97 (1%) Frame = +3 Query: 240 PRRRSDLSHMNTPAKVSGHKGPEE-LSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASI 416 P +D+ H+NT + V G+ ++ L A+ ADVV+IPAGVPRKPGMTRDDLF TNASI Sbjct: 66 PGVAADIGHINTTSNVVGYAPDDKGLEKALNGADVVIIPAGVPRKPGMTRDDLFATNASI 125 Query: 417 VRDIALSIAQNAPKAIVAIITNPVNSTVPIALKCSRR 527 VRD+A + + P+A ++TNPVNSTVPI K R Sbjct: 126 VRDLAFAAGETCPEAKYLVVTNPVNSTVPIFKKALER 162 Score = 52.0 bits (119), Expect = 8e-08 Identities = 25/59 (42%), Positives = 35/59 (59%), Gaps = 1/59 (1%) Frame = +2 Query: 512 EVLKKAGVYDPNRVLGVTTLDVSRCHLRRRDQWRGPNSVA-VPVIGGHSGITIIPILSQ 685 + L++ GV+ P + GVTTLD R G + +PV+GGHSG TI+P+LSQ Sbjct: 158 KALERVGVHQPKHLFGVTTLDSVRASRFTSQVTNGKAELLHIPVVGGHSGATIVPLLSQ 216 Score = 50.8 bits (116), Expect = 2e-07 Identities = 28/53 (52%), Positives = 34/53 (64%) Frame = +1 Query: 97 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAA 255 ++FSTTS R FK IGQPL++LLK N V+ LAL+DI PGVAA Sbjct: 19 RSFSTTSSRAFKVAVLGAGGGIGQPLSMLLKLNDKVSELALFDIRG-APGVAA 70 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 53.6 bits (123), Expect = 3e-08 Identities = 34/97 (35%), Positives = 49/97 (50%) Frame = +3 Query: 327 KDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPI 506 KDA VVI AG +KPG TR DL N SI ++I + + AI+ + TNPV+ Sbjct: 86 KDATAVVITAGKNQKPGETRMDLLKANISIFKEILREVTKYTKDAILLVATNPVDVLTYA 145 Query: 507 ALKCSRRRECTTRTACSASPLWTYRAATFVGEINGVD 617 LK + R S + + T R +G++ G+D Sbjct: 146 TLKLTGFP--AERVIGSGTIIDTARFQYLIGKLYGLD 180 >SPAC23H3.05c |swd1||COMPASS complex subunit Swd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 398 Score = 28.3 bits (60), Expect = 1.2 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = -3 Query: 446 LSNGQSNVTDNGSISVE*VITGHSRFTGYSCW 351 L NG + D + SV V+TGH+R CW Sbjct: 43 LVNGSVVIWDLSTFSVSRVLTGHTRAIQSVCW 74 >SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1462 Score = 27.5 bits (58), Expect = 2.1 Identities = 16/63 (25%), Positives = 28/63 (44%) Frame = -1 Query: 307 SGPLWPLTLAGVFMWERSLRRRGSQALCRIKPAWSPEDSASVEGPKAGRYHRRHQRPPL* 128 SG L+ L FM++ L R ++ ++ +WS +D+ + H P L Sbjct: 794 SGLLFSLRAHDTFMFDGLLDRLNEESRTKLVSSWSKQDAFDYSKSSTHQEHLSKNLPTLN 853 Query: 127 SSS 119 +SS Sbjct: 854 TSS 856 >SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase|Schizosaccharomyces pombe|chr 2|||Manual Length = 362 Score = 27.1 bits (57), Expect = 2.8 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +3 Query: 75 PCCTKWCKELFHHITEEL*SGGRWCR 152 P C WC ++ IT+ + G WCR Sbjct: 319 PDCKSWCGIIYQFITDS--NVGMWCR 342 >SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1233 Score = 25.8 bits (54), Expect = 6.4 Identities = 15/44 (34%), Positives = 22/44 (50%) Frame = -3 Query: 296 VAADLGWGVHVGKVAATPGVTGAMSYKASLVTRGFCFSRRAKGW 165 VA DL + + A T + G + +K L+T G +R AK W Sbjct: 631 VARDLSYNKRLN--AKTVTLEGTVIHKTGLITGGSSNNRSAKHW 672 >SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharomyces pombe|chr 3|||Manual Length = 350 Score = 25.8 bits (54), Expect = 6.4 Identities = 11/33 (33%), Positives = 17/33 (51%) Frame = +2 Query: 617 PNSVAVPVIGGHSGITIIPILSQCQPALKLSDR 715 P +P+IGGH G ++ + LK+ DR Sbjct: 58 PLPAKMPLIGGHEGAGVVVKVGAGVTRLKIGDR 90 >SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 489 Score = 25.8 bits (54), Expect = 6.4 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = +3 Query: 63 KTCRPCCTKWCKELFHHITEEL*SGGRWCRRWY 161 K C+ C + C+ +HHI E+L CRR Y Sbjct: 33 KPCQ-CGYRVCRFCWHHIKEDLNGRCPACRRLY 64 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,949,774 Number of Sequences: 5004 Number of extensions: 62292 Number of successful extensions: 173 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 345237368 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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