Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021136
(805 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 122 6e-29
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 105 6e-24
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 29 0.77
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 29 0.77
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 27 2.4
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 27 3.1
SPAC1786.01c ||SPAC31G5.20c|triacylglycerol lipase|Schizosacchar... 27 3.1
SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomy... 27 4.1
SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 27 4.1
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 26 5.5
SPAC17C9.06 |sam50||SAM complex subunit Sam50 |Schizosaccharomyc... 26 7.2
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 26 7.2
SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 9.5
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 25 9.5
SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyce... 25 9.5
SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|... 25 9.5
SPAC1039.01 |||amino acid permease, unknown 5|Schizosaccharomyce... 25 9.5
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 122 bits (294), Expect = 6e-29
Identities = 79/195 (40%), Positives = 110/195 (56%), Gaps = 10/195 (5%)
Frame = +2
Query: 251 GSRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV---NPTLSAPLEDVVNS 421
G + TLIPGDG+G E AV E+FK A++P++FE + + N + L + + S
Sbjct: 18 GGKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQS 77
Query: 422 IAVNKICIKGILATPDFSHTGELQTLNMKSVMPWIFTLTWYSEVIAQREVQAPGRGLHHH 601
+ NK+ +KGIL TP F G + N+ L Y+ ++ + + PG H
Sbjct: 78 LKRNKVGLKGILFTP-FEKGGHT-SFNVALRKE----LDIYASLVLIKNI--PGFKTRHD 129
Query: 602 -------QRTDRREYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRR 760
+ EYS LEH+SVPGVVE LKIIT KS+RIA+FAFD+A+++G +
Sbjct: 130 NVDFAIIRENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNGRKSVTCI 189
Query: 761 PKANIMKLGDGLFLR 805
KANIMKL DGLF R
Sbjct: 190 HKANIMKLADGLFRR 204
Score = 32.3 bits (70), Expect = 0.083
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +3
Query: 546 VKSLPNVKCRHQDVDCIIIREQTE 617
+K++P K RH +VD IIRE TE
Sbjct: 118 IKNIPGFKTRHDNVDFAIIRENTE 141
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 105 bits (253), Expect = 6e-24
Identities = 62/188 (32%), Positives = 97/188 (51%), Gaps = 2/188 (1%)
Frame = +2
Query: 245 NKGSRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV--NPTLSAPLEDVVN 418
N T+I GDG+GPE+ +V+ +FKAA +P+++E + N T + P +D
Sbjct: 41 NANGNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGTTTIP-DDAKE 99
Query: 419 SIAVNKICIKGILATPDFSHTGELQTLNMKSVMPWIFTLTWYSEVIAQREVQAPGRGLHH 598
S+ NK+ +KG LATP L ++ + + + +
Sbjct: 100 SVRKNKVALKGPLATP-IGKGHVSMNLTLRRTFGLFANVRPCVSITGYKTPYDNVNTVLI 158
Query: 599 HQRTDRREYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRRPKANIM 778
+ T+ EYS +EHE +PGVV+ +K+IT A SER+ ++AF YA + G KA IM
Sbjct: 159 RENTEG-EYSGIEHEVIPGVVQSIKLITRAASERVIRYAFQYARQTGKNNITVVHKATIM 217
Query: 779 KLGDGLFL 802
++ DGLFL
Sbjct: 218 RMADGLFL 225
>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
Leu1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 371
Score = 29.1 bits (62), Expect = 0.77
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2
Query: 263 KCTLIPGDGVGPELVYAVQEVFK 331
K ++PGD +GPE+V + EV K
Sbjct: 5 KIVVLPGDHIGPEIVASALEVLK 27
>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
Spt6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1365
Score = 29.1 bits (62), Expect = 0.77
Identities = 22/93 (23%), Positives = 44/93 (47%), Gaps = 12/93 (12%)
Frame = -1
Query: 442 TNLVNSDRVYDVFEWCTKCW-IHFRKEKGFKVHWNAG----------RLENFLYSVDKLR 296
T L++ + ++++F CTK W +H +K+ K++ + G + L ++D L
Sbjct: 355 TPLLSQNDLWNIFFLCTKFWSLHSKKQDILKLYSDLGINDDLVVPFCEAASSLDAIDDLN 414
Query: 295 TYT-VPRNQGAFDTTSFVALGCRAPNGA*HTFF 200
Y ++ D + G R P G+ ++FF
Sbjct: 415 DYIHFTYSEQIRDRALLMGTGLRRPQGSKYSFF 447
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 27.5 bits (58), Expect = 2.4
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = -1
Query: 439 NLVNSDRVYDVFEWCTKCWIHFRKEKGFKVHW 344
N VN+ VY +F WI+ R FK+ W
Sbjct: 269 NYVNTVIVYPIFVIFVFVWIYMRHYLNFKIMW 300
>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1098
Score = 27.1 bits (57), Expect = 3.1
Identities = 15/56 (26%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
Frame = +2
Query: 371 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKS-VMPWIFTL 535
S++N A LED N +C++ +A S LQ + +S +M + + L
Sbjct: 541 SQLNVFTKASLEDKQKKEKYNLLCVRSCIAIDSISWISSLQGVKFRSKLMAYFYPL 596
>SPAC1786.01c ||SPAC31G5.20c|triacylglycerol
lipase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 630
Score = 27.1 bits (57), Expect = 3.1
Identities = 16/64 (25%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = +2
Query: 566 EVQAPGRGLHHHQRTDRREYSALEHE-SVPGVVECLKIITAAKSERIAKFAFDYAVKDGP 742
+V +P + +++ D +SA+ +VPG++ + ++T ++S R+ F KDG
Sbjct: 372 DVHSPPKLINYLTSPDTVIWSAVIASCAVPGILNPIPLMTRSQSHRLIPHNFGNRFKDGS 431
Query: 743 QEGD 754
D
Sbjct: 432 LRTD 435
>SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 794
Score = 26.6 bits (56), Expect = 4.1
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +2
Query: 596 HHQRTDRREYSALEHESVPGVVECLKIITAAKSER 700
H DRR Y + + VPGV E + +T K E+
Sbjct: 62 HEGIPDRRAYVIVLEKEVPGVFEGIVNLTTGKIEK 96
>SPAC19G12.02c |pms1||MutL family mismatch-repair protein
Pms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 794
Score = 26.6 bits (56), Expect = 4.1
Identities = 21/70 (30%), Positives = 34/70 (48%)
Frame = +2
Query: 296 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 475
P A+Q++ S P+D + F ++N +LS +D+V S A+ K K I D S
Sbjct: 533 PSKTAALQKLKFFQSRPLDGLNKFSKKINISLSGVQKDIVRSDALLKFSNK-IGVVHDIS 591
Query: 476 HTGELQTLNM 505
+ LN+
Sbjct: 592 DENQEDHLNL 601
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 26.2 bits (55), Expect = 5.5
Identities = 22/85 (25%), Positives = 32/85 (37%), Gaps = 9/85 (10%)
Frame = +2
Query: 224 WSPATEGNKGSRIKCTLIPGDGVGPELVYAVQEVFKAASIPVD-------FESFFFSEVN 382
W T G G DG GP L + E K PVD F+ S+++
Sbjct: 395 WDDFTNGRFGDPRSPAYGEIDGYGPTLHFPPSEALKLWGYPVDESDITSLFQKHIMSDIS 454
Query: 383 --PTLSAPLEDVVNSIAVNKICIKG 451
P + P+E +IA + + G
Sbjct: 455 AIPWIDEPVEVETKTIAKYLLKLNG 479
>SPAC17C9.06 |sam50||SAM complex subunit Sam50 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 475
Score = 25.8 bits (54), Expect = 7.2
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -3
Query: 764 WDGGHLLAAHLSRRSRKRISQYARISLR 681
W G HLL+ +L R +++YA S+R
Sbjct: 228 WSGEHLLSQNLLWRQVTHLTEYASPSVR 255
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 25.8 bits (54), Expect = 7.2
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = -1
Query: 289 TVPRNQGAFDTTSFVALGCRAPNGA*HTFFSVVTE 185
T P AF T+ FV+ A NG + ++V +E
Sbjct: 154 TTPATTNAFGTSGFVSSNANAVNGTANPPYAVTSE 188
>SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 867
Score = 25.4 bits (53), Expect = 9.5
Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = +2
Query: 365 FFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTG-ELQTLNMKSVMPWIFTLTW 541
F S NPT+ L + A++K I LAT +H G + +++ + ++ +F W
Sbjct: 487 FSSMWNPTIVLLLGGFTIAAALSKYHIAKRLATSILAHAGRKPRSVLLTNMFVAMFASMW 546
Query: 542 YSEVIA 559
S V A
Sbjct: 547 ISNVAA 552
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 25.4 bits (53), Expect = 9.5
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2
Query: 257 RIKCTLIPGDGVGPELVYAVQEVFK 331
RI LIP DG+G E+V A + + +
Sbjct: 6 RIVLGLIPADGIGKEVVPAARRLME 30
>SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 415
Score = 25.4 bits (53), Expect = 9.5
Identities = 17/48 (35%), Positives = 24/48 (50%), Gaps = 3/48 (6%)
Frame = +3
Query: 636 NMNPFPAWWSV*RSSPQRNPSVLR---NSLSTTP*KMGRKKVTAVPRP 770
N P P S SSP R PS+++ S S P + ++ + VPRP
Sbjct: 353 NSEPNPKLDSQPNSSPVRRPSLIKVKTASTSVLPTSILQRPPSIVPRP 400
>SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit
Alp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 25.4 bits (53), Expect = 9.5
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = -2
Query: 549 SLYHVSVKIQGITDFMFRV 493
S+YHVS+K +G++ F R+
Sbjct: 27 SVYHVSLKSEGVSPFSDRI 45
>SPAC1039.01 |||amino acid permease, unknown 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 567
Score = 25.4 bits (53), Expect = 9.5
Identities = 31/138 (22%), Positives = 60/138 (43%), Gaps = 12/138 (8%)
Frame = +2
Query: 299 ELVYAVQEV--FKAASIPVDFESFFFSEVN----PTLSAPLEDVVNSIAVNKICIK-GIL 457
+ V++V V F A ++P+ FF + + P + +AV+ + + IL
Sbjct: 429 DAVFSVGAVAAFIAFTVPIAIRVFFTKDADFRRGPWNLGKFSRPIGLLAVSFVALMIPIL 488
Query: 458 ATPDFSHTGELQTLNMKSVM---PWIFTLTWYSEVIAQREVQAPGRGLHHHQRTDRREYS 628
P + Q +N ++ P +FTL WY+ + A++ + P + H++R
Sbjct: 489 CFPSVKNP-TAQEMNWTCLVYGGPMLFTLVWYA-ISARKWFKGP-KASAHYKRPGEESSD 545
Query: 629 ALE--HESVPGVVECLKI 676
+E +P + LKI
Sbjct: 546 IVEGVQADIPSSSDQLKI 563
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,420,685
Number of Sequences: 5004
Number of extensions: 74755
Number of successful extensions: 221
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -